- Genetic linkage map construction and quantitative trait loci mapping of agronomic traits in Gloeostereum incarnatum
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Wan-Zhu Jiang , Fang-Jie Yao , Li-Xin Lu , Ming Fang , Peng Wang , You-Min Zhang , Jing-Jing Meng , Jia Lu , Xiao-Xu Ma , Qi He , Kai-Sheng Shao
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J. Microbiol. 2021;59(1):41-50. Published online November 17, 2020
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DOI: https://doi.org/10.1007/s12275-021-0242-5
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Gloeostereum incarnatum is an edible medicinal mushroom
widely grown in China. Using the whole genome of G. incarnatum,
simple sequence repeat (SSR) markers were developed
and synthetic primers were designed to construct its
first genetic linkage map. The 1,048.6 cm map is composed of
10 linkage groups and contains 183 SSR markers. In total,
112 genome assembly sequences were anchored, representing
16.43 Mb and covering 46.41% of the genome. Selfing
populations were used for quantitative trait loci (QTL) targeting,
and the composite interval mapping method was used
to co-localize the mycelium growth rate (potato dextrose agar
and sawdust), growth period, yield and fruiting body length,
and width and thickness. The 14 QTLs of agronomic traits
had LOD values of 3.20–6.51 and contribution rates of 2.22–
13.18%. No linkage relationship was found between the mycelium
growth rate and the growth period, but a linkage relationship
was observed among the length, width and thickness
of the fruiting bodies. Using NCBI’s BLAST alignment,
the genomic sequences corresponding to the QTL regions
were compared, and a TPR-like protein candidate gene was
selected. Using whole-genome data, 138 candidate genes were
found in four sequence fragments of two SSR markers located
in the same scaffold. The genetic map and QTLs established
in this study will aid in developing selective markers
for agronomic traits and identifying corresponding genes,
thereby providing a scientific basis for the further gene mapping
of quantitative traits and the marker-assisted selection
of functional genes in G. incarnatum breeding programs.
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Citations
Citations to this article as recorded by  - Dual genetic loci and flavonoid metabolism orchestrate fruiting body coloration in Flammulina filiformis: a multi-omic roadmap for fungal pigmentation
Zhi-Wen Lv, Jin-Xiang Zhang, Meng-Han Nie, Chuan-Zheng Wei, Tuo Zhang, Fei Liu, Zhi-Lin Ling, Xin-Yue Chen, Xiao-Ping Wu, Yong-Xin Tao, Bao-Gui Xie, Rui-Lin Zhao
Journal of Advanced Research.2026; 87: 309. CrossRef - Genetic dissection of seed quality traits in mungbean through QTL-seq of recombinant inbred line populations
Chunxiu Ren, Jiaqi Qiao, Lipan Qiao, Bin Li, Xiaopeng Hao, Xin Guo, Fan Yang, Dongao Huo
Journal of Plant Physiology.2026; 322: 154795. CrossRef - Biochemistry and Chemoinformatics Guided Classification of Hirsutane Sesquiterpenes Isolated from Mushroom
Yaping Liu, Jumpei Nishishita, Chengwei Liu, Hideaki Oikawa, Atsushi Minami
JACS Au.2025; 5(2): 740. CrossRef - Growth-Rate Related Quantitative Trait Locus Analysis of Monokaryotic Isolates of Grifola albicans f. huishuhua (Maitake)
Panpan Zhang, Junling Wang, Guojie Li, Shangshang Xiao, Lei Sun, Xiao Li, Jinghua Tian, Ming Li, Shoumian Li
Journal of Fungi.2025; 11(12): 865. CrossRef - Dicarboxylic Amino Acid Permease 7219 Regulates Fruiting Body Type of Auricularia heimuer
Jia Lu, Lixin Lu, Fangjie Yao, Ming Fang, Xiaoxu Ma, Jingjing Meng, Kaisheng Shao
Journal of Fungi.2023; 9(9): 876. CrossRef - Identification of quantitative trait loci for growth traits in red swamp crayfish (Procambarus clarkii)
Junxiao Sun, Cuirong Luo, Bo Peng, Guohui Peng, Yunfei Tan, Xufeng Bai
Aquaculture and Fisheries.2023; 8(6): 727. CrossRef - Improved Genetic Map and Localization of Quantitative Trait Loci for Quality Traits in Auricularia heimuer
Jia Lu, Ming Fang, Fangjie Yao, Lixin Lu, Xiaoxu Ma, Jingjing Meng, Kaisheng Shao
Horticulturae.2023; 9(7): 763. CrossRef - Assemblies of the genomes of parasitic wasps using meta-assembly and scaffolding with genetic linkage
Kameron T Wittmeyer, Sara J Oppenheim, Keith R Hopper, J Hesselberth
G3 Genes|Genomes|Genetics.2022;[Epub] CrossRef - EST-SSR Primer Development and Genetic Structure Analysis of Psathyrostachys juncea Nevski
Zhen Li, Lan Yun, Zhiqi Gao, Tian Wang, Xiaomin Ren, Yan Zhao
Frontiers in Plant Science.2022;[Epub] CrossRef - Analysis of the Genome Sequence of Strain GiC-126 of Gloeostereum incarnatum with Genetic Linkage Map
Wan-Zhu Jiang, Fang-Jie Yao, Ming Fang, Li-Xin Lu, You-Min Zhang, Peng Wang, Jing-Jing Meng, Jia Lu, Xiao-Xu Ma, Qi He, Kai-Sheng Shao, Asif Ali Khan, Yun-Hui Wei
Mycobiology.2021; 49(4): 406. CrossRef
- Construction of a genetic linkage map and QTL mapping of agronomic traits in Auricularia auricula-judae
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Li-Xin Lu , Fang-Jie Yao , Peng Wang , Ming Fang , You-Min Zhang , Wei-Tong Zhang , Xiang-Hui Kong , Jia Lu
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J. Microbiol. 2017;55(10):792-799. Published online September 28, 2017
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DOI: https://doi.org/10.1007/s12275-017-7241-6
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1,031
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16
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Abstract
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Auricularia auricula-judae is a traditional edible fungus that
is cultivated widely in China. In this study, a genetic linkage
map for A. auricula-judae was constructed using a mapping
population consisting of 138 monokaryons derived from a
hybrid strain (A119-5). The monokaryotic parent strains
A14-5 and A18-119 were derived from two cultivated varieties,
A14 (Qihei No. 1) and A18 (Qihei No. 2), respectively.
In total, 130 simple sequence repeat markers were mapped.
These markers were developed using the whole genome sequence
of A. auricula-judae and amplified in A14-5, A18-
119, and the mapping population. The map consisted of 11
linkage groups (LGs) spanning 854 cM, with an average interval
length of 6.57 cM. A testcross population was derived
from crossing between the monokaryon A184-57 (from the
wild strain A184 as a tester strain) and the mapping population.
Important agronomic trait-related QTLs, including
mycelium growth rate on potato dextrose agar for the mapping
population, mycelium growth rate on potato dextrose
agar and sawdust for the testcross population, growth period
(days from inoculation to fruiting body harvesting), and yield
for the testcross population, were identified using the composite
interval mapping method. Six mycelium growth raterelated
QTLs were identified on LG1 and LG4, two growth
period-related QTLs were identified on LG2, and three yieldrelated
QTLs were identified on LG2 and LG6. The results
showed no linkage relationship between mycelium growth
rate and growth period. The present study provides a foundation
for locating genes for important agronomic characteristics
in A. auricula-judae in the future.
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Citations
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Food Bioscience.2026; 78: 108565. CrossRef - Identification of the major QTL related to stipe length based on QTL mapping and BSA‐Seq in oyster mushroom
Zeyin Wu, Chenyang Huang, Yanjiao Ma, Fangjie Yao, Mengran Zhao, Lijiao Zhang
Journal of the Science of Food and Agriculture.2026; 106(7): 3995. CrossRef - Growth-Rate Related Quantitative Trait Locus Analysis of Monokaryotic Isolates of Grifola albicans f. huishuhua (Maitake)
Panpan Zhang, Junling Wang, Guojie Li, Shangshang Xiao, Lei Sun, Xiao Li, Jinghua Tian, Ming Li, Shoumian Li
Journal of Fungi.2025; 11(12): 865. CrossRef - RETRACTED: Exploring the potential of black fungus, Auricularia auricula, as a feed additive in African catfish, Clarias gariepinus, farming
Lee Seong Wei, Alvin Amos Adrian Susin, Albaris B. Tahiluddin, Liew Vui Kien, Wendy Wee
Heliyon.2024; 10(13): e33810. CrossRef - Evaluation of Drought Tolerance and Trehalose Response in Auricularia heimuer
Jian Sun, Fangjie Yao, Lixin Lu, Youmin Zhang, Ming Fang, Xiaoxu Ma, Kaisheng Shao, Xu Sun
Horticulturae.2024; 10(12): 1312. CrossRef -
Medicinal Value, Genetic Diversity, and Genetic Relationship Analysis of Auricularia cornea (Agaricomycetes) Based on ITS, ISSR, and SRAP Markers
Ping Du, Hong-Yan He, Ni-Ya Wu, Tianxu Cao, Bao-Kai Cui
International Journal of Medicinal Mushrooms.2024; 26(5): 43. CrossRef - Velvet Family Members Regulate Pigment Synthesis of the Fruiting Bodies of Auricularia cornea
Xiaoxu Ma, Lixin Lu, Youmin Zhang, Ming Fang, Kaisheng Shao, Xu Sun, Fangjie Yao, Peng Wang
Journal of Fungi.2023; 9(4): 412. CrossRef - The polysaccharides from Auricularia auricula alleviate non-alcoholic fatty liver disease via modulating gut microbiota and bile acids metabolism
Yifan Shu, Yujie Huang, Wei Dong, Xia Fan, Yi Sun, Guijie Chen, Xiaoxiong Zeng, Hong Ye
International Journal of Biological Macromolecules.2023; 246: 125662. CrossRef - Detection of quantitative trait loci underlying fruiting body and yield-related traits in Hericium erinaceus
Wenbing Gong, Xiaoya Song, Chunliang Xie, Yingjun Zhou, Zuohua Zhu, Chao Xu, Yuande Peng
Scientia Horticulturae.2022; 293: 110729. CrossRef - Analysis of the Genome Sequence of Strain GiC-126 of Gloeostereum incarnatum with Genetic Linkage Map
Wan-Zhu Jiang, Fang-Jie Yao, Ming Fang, Li-Xin Lu, You-Min Zhang, Peng Wang, Jing-Jing Meng, Jia Lu, Xiao-Xu Ma, Qi He, Kai-Sheng Shao, Asif Ali Khan, Yun-Hui Wei
Mycobiology.2021; 49(4): 406. CrossRef - SNP-Based Genetic Linkage Map and Quantitative Trait Locus Mapping Associated with the Agronomically Important Traits of Hypsizygus marmoreus
Youn-Lee Oh, In-Geol Choi, Kab-Yeul Jang, Min-Seek Kim, Min ji Oh, Ji-Hoon Im
Mycobiology.2021; 49(6): 589. CrossRef - Genetic linkage map construction and quantitative trait loci mapping of agronomic traits in Gloeostereum incarnatum
Wan-Zhu Jiang, Fang-Jie Yao, Li-Xin Lu, Ming Fang, Peng Wang, You-Min Zhang, Jing-Jing Meng, Jia Lu, Xiao-Xu Ma, Qi He, Kai-Sheng Shao
Journal of Microbiology.2021; 59(1): 41. CrossRef - Polysaccharides from Auricularia auricula: Preparation, structural features and biological activities
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Carbohydrate Polymers.2020; 247: 116750. CrossRef - Genome Sequence Analysis of Auricularia heimuer Combined with Genetic Linkage Map
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Frontiers in Microbiology.2020;[Epub] CrossRef - Comparative transcriptome analysis reveals relationship of three major domesticated varieties of Auricularia auricula-judae
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Scientific Reports.2019;[Epub] CrossRef
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