- In vitro effects of N-acetyl cysteine alone and in combination with antibiotics on Prevotella intermedia
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Ji-Hoi Moon , Eun-Young Jang , Kyu Sang Shim , Jin-Yong Lee
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J. Microbiol. 2015;53(5):321-329. Published online May 3, 2015
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DOI: https://doi.org/10.1007/s12275-015-4500-2
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 Abstract
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N-acetyl cysteine (NAC) is an antioxidant that possesses
anti-inflammatory activities in tissues. In the field of dentistry,
NAC was demonstrated to prevent the expression of
LPS-induced inflammatory mediators in phagocytic cells
and gingival fibroblasts during the inflammatory process,
but the effect of NAC on oral pathogens has been rarely
studied. Here, we examined the effect of NAC against planktonic
and biofilm cells of Prevotella intermedia, a major oral
pathogen. NAC showed antibacterial activity against the
planktonic P. intermedia with MIC value of 3 mg/ml and
significantly decreased biofilm formation by the bacterium
even at sub MIC. NAC did not affect the antibiotic susceptibility
of planktonic P. intermedia, showing indifference (fractional
inhibitory concentration index of 0.5?) results against
the bacterium in combination with ampicillin, ciprofloxacin,
tetracycline or metronidazole. On the other hand, viability
of the pre-established bacterial biofilm exposed to the antibiotics
except metronidazole was increased in the presence
of NAC. Collectively, NAC may be used for prevention of
the biofilm formation by P. intermedia rather than eradication
of the pre-established bacterial biofilm. Further studies
are required to explore antibacterial and anti-biofilm activity
of NAC against mixed population of oral bacteria and its
modulatory effect on antibiotics used for oral infectious
diseases.
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Citations
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