- Green Fluorescent Protein as a Marker for Monitoring a Pentachlorophenol Degrader Sphingomonas chlorophenolica ATCC39723
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Eun-Taex Oh , Jae-Seong So , Byung-Hyuk Kim , Jong-Sul Kim , Sung-Cheol Koh
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J. Microbiol. 2004;42(3):243-247.
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DOI: https://doi.org/2081 [pii]
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Sphingomonas chlorophenolica ATCC39723 was successfully labeled with the gfp (green fluorescent protein) gene inserted into the pcpB gene by homologous recombination. As the gfp recombinant was easily distinguished from other indigenous organisms, the population of gfp recombinant was monitored after being released into the soil microcosms. Their population density dropped from 108 to 106 (cfu/ml) in the non-sterilized soil microcosms during the first 6 days. Moreover, the gfp recombinant was not detected even at lower dilution rates after a certain time period. The recombinant, however, survived for at least 28 days in the sterilized soil microcosms. Although the gfp recombinant did not degrade pentachlorophenol (PCP), this experiment showed the possibility of using gfp as a monitoring reporter system for S. chlorophenolica ATCC39723 and potentially other species of Sphingomonas.
- Plant Terpene-Induced Expression of Multiple Aromatic Ring Hydroxylation Oxygenase Genes in Rhodococcus sp. Strain T104
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Byung-Hyuk Kim , Eun-Taex Oh , Jae-Seong So , Yeonghee Ahn , Sung-Cheol Koh
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J. Microbiol. 2003;41(4):349-352.
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Recent studies have shown that some of the PCB (polychlorinated biphenyl)-degraders are able to effectively degrade PCB in the presence of monoterpenes, which act as inducers for the degradation pathway. Rhodococcus sp. T104, an effective PCB degrader, has been shown to induce the degradation pathway by utilizing limonenes, cymenes, carvones, and pinenes as sole carbon sources which can be found in the natural environment. Moreover, the strain T104 proved to possess three separate oxidation pathways of limonene, biphenyl, and phenol. Of these three, the limonene can also induce the biphenyl degradation pathway. In this work, we report the presence of three distinct genes for aromatic oxygenase, which are putatively involved in the degradation of aromatic substrates including biphenyl, limonene, and phenol, through PCR amplification and denaturing gradient gel electrophoresis (DGGE). The genes were differentially expressed and well induced by limonene, cymene, and plant extract A compared to biphenyl and/or glucose. This indicates that substrate specificity must be taken into account when biodegradation of the target compounds are facilitated by the plant natural substrates.
- Isolation and Identification of a Pentachloronitrobenzene (PCNB) Degrading Bacterium Alcaligenes xylosoxidans PCNB-2 from Agricultural Soil
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Sung-Kyu Shin , Jang-Eok Kim , Gi-Seok Kwon , Jin-Wook Kwon , Eun-Taex Oh , Jae-Seong So , Sung-Cheol Koh
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J. Microbiol. 2003;41(2):165-168.
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We report a new PCNB-degrading strain (PCNB-2) that is able to utilize and grow on PCNB (100 ppm) as a sole carbon source. This strain was identified as Alcaligenes xylosoxidans based upon 16S rDNA sequence analysis, API 20 NE tests and cell membrane lipid analysis. The new PCNB degrader Alcali-genes xylosoxidans PCNB-2 could find use in bioremediation of PCNB, which is environmentally persistent.
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