Journal of Microbiology

Eun-Jin Yang

3 Articles

Transcriptomic Insights into Archaeal Nitrification in the Amundsen Sea Polynya, Antarctica: Joo-Han Gwak , Samuel Imisi Awala , So-Jeong Kim , Sang-Hoon Lee , Eun-Jin Yang , Jisoo Park , Jinyoung Jung , Sung-Keun Rhee; J. Microbiol. 2023;61(11):967-980. Published online December 7, 2023; DOI: https://doi.org/10.1007/s12275-023-00090-0

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Antarctic polynyas have the highest Southern Ocean summer primary productivity, and due to anthropogenic climate change, these areas have formed faster recently. Ammonia-oxidizing archaea (AOA) are among the most ubiquitous and abundant microorganisms in the ocean and play a primary role in the global nitrogen cycle. We utilized metagenomics and metatranscriptomics to gain insights into the physiology and metabolism of AOA in polar oceans, which are associated with ecosystem functioning. A polar-specific ecotype of AOA, from the “Candidatus Nitrosomarinus”-like group, was observed to be dominant in the Amundsen Sea Polynya (ASP), West Antarctica, during a succession of summer phytoplankton blooms. AOA had the highest transcriptional activity among prokaryotes during the bloom decline phase (DC). Metatranscriptomic analysis of key genes involved in ammonia oxidation, carbon fixation, transport, and cell division indicated that this polar AOA ecotype was actively involved in nitrification in the bloom DC in the ASP. This study revealed the physiological and metabolic traits of this key polar-type AOA in response to phytoplankton blooms in the ASP and provided insights into AOA functions in polar oceans.

Citations

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Bulk metagenomics and machine learning unravels nitrogen metabolism patterns in extreme-temperature marine environments
Zheng Guo, Yong-Guang Li, Xiao-Lin Liu, Zhao-Jie Teng, Qi-Long Qin, Qian-Qian Cha, Zhi-Bin Wang, Shou-Qing Ni
Bioresource Technology.2026; 441: 133551. CrossRef
Archaea as a resource for sustainable biotechnology: From extremophiles to valuable products
Alaa Hassanien, Imen Saadaoui, Sami Sayadi
Biochimie.2026; 250: 17. CrossRef
Alleviated photoinhibition on nitrification in the Indian Sector of the Southern Ocean
Lingfang Fan, Min Chen, Zifei Yang, Minfang Zheng, Yusheng Qiu
Acta Oceanologica Sinica.2024; 43(7): 52. CrossRef

Freezing Seawater for the Long-term Storage of Bacterial Cells for Microscopic Enumeration: Jung-Ho Jung-Ho , Eun-Jin Yang; J. Microbiol. 2006;44(6):699-699.

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Freezing Seawater for the Long-term Storage of Bacterial Cells for Microscopic Enumeration: Jung-Ho Hyun , Eun-Jin Yang; J. Microbiol. 2003;41(3):262-265.

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Abstract PDF: Although enumerating bacterial cells is a fundamental step in understanding microbial ecosystems in marine environments, substantial decrease in bacterial counts with increasing sample storage time hampers the accurate estimation of bacterial biomass. We compared the variations in bacterial cell numbers caused by freezing and thawing of sample bottles or slides. Bacterial counts of seawater samples frozen only once in a sampling bottle yielded approximately 95% of the original numbers after 90 days, whereas 80% of the original count was obtained for samples prepared on slides. Only 67% and 58% of the original counts were recovered in samples repeatedly frozen and thawed in bottles or on slides, respectively. The results indicated that freezing a seawater sample in a bottle increased the consistency of the epifluorescence microscopic enumeration of bacterial cells.

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