- Genomic and physiological analysis of C50 carotenoid-producing novel Halorubrum ruber sp. nov.
-
Chi Young Hwang , Eui-Sang Cho , Won Jong Rhee , Eunjung Kim , Myung-Ji Seo
-
J. Microbiol. 2022;60(10):1007-1020. Published online August 26, 2022
-
DOI: https://doi.org/10.1007/s12275-022-2173-1
-
-
964
View
-
8
Download
-
11
Web of Science
-
12
Crossref
-
 Abstract
 PDF
-
A novel haloarchaeal species designated as MBLA0099T was
isolated from seawater near Yeongheung Island. Cells were
Gram-negative, non-motile, red-pigmented, and rod-shaped.
They grew at 10–45°C, within pH 5.5–9.0, and between 7.5%
and 30% NaCl concentrations. Cells were able to grow without
Mg2+ and were lysed in distilled water. The size of the
whole-genome and G + C content of DNA was 3.02 Mb and
68.9 mol%, respectively. Phylogenetic analysis shows that
the strain MBLA0099T belongs to the genus Halorubrum.
The average nucleotide and amino acid identity, and in silico
DNA-DNA hybridization values were below the species delineation
threshold. Pan-genomic analysis revealed that 3.2%
of all genes present in strain MBLA0099T were unique to the
strain. The red carotenoid produced by strain MBLA0099T
was subjected to spectrometric and chromatographic analyses
and confirmed to be bacterioruberin as C50 carotenoid.
Mevalonic acid, terpenoid backbone, and carotenoid biosynthesis
pathway were annotated for strain MBLA0099T. The
C50 carotenoid production by strain MBLA0099T was also enhanced
under various stress conditions including relatively
netural pH, high oxidative and salinity conditions. Additionally,
the strain MBLA0099T-derived bacterioruberin showed
the antioxidant activity with EC50 value of 12.29 μg/ml, based
on the evaluation of DPPH free radical scavenging activity.
The present study would be the first report on the identification
of C50 carotenoid from the strain MBLA0099T representing
a novel species of the genus Halorubrum, for which
the name Halorubrum ruber sp. nov. is proposed. The typestrain
used was MBLA0099T (= KCTC 4296T = JCM 34701T).
-
Citations
Citations to this article as recorded by  - Genomic Insights into Bacterioruberin and Halorhodopsin Biosynthetic Genes in 12 Halophilic Archaea Isolated from Korean Solar Salterns
Dongseok Lee, Chi Young Hwang, Eui-Sang Cho, Young-Hyun You, Myung-Ji Seo
Journal of Microbiology and Biotechnology.2026;[Epub] CrossRef - Recent Advances in Understanding Bio-Compounds from Haloarchaea
Chi Young Hwang, Myung-Ji Seo
Journal of Microbiology and Biotechnology.2026;[Epub] CrossRef -
Genomic insights into
Halorubrum ezzemoulense
strain TC23: Genetic basis for halophilic traits and biotechnological potential
Fevziye Işıl Kesbiç
Science Progress.2025;[Epub] CrossRef - Genome-based classification of Halonovum salinarum gen. nov., sp. nov., and Halonovum rutilum sp. nov., two novel halophilic archaea isolated from a solar saltern
Chi Young Hwang, Eui-Sang Cho, Ki-Eun Lee, Eun-Young Lee, Myung-Ji Seo
BMC Microbiology.2025;[Epub] CrossRef - Combined Environmental Stress Responses and Their Influence on Bacterioruberin Biosynthesis: A Comprehensive Review
Devi Prasad Panda
Next Gen Multidisciplinary Research.2025; 1(2): 17. CrossRef - Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potential
Chi Young Hwang, Eui-Sang Cho, Sungjun Kim, Kyobum Kim, Myung-Ji Seo
Microbial Cell Factories.2024;[Epub] CrossRef - Microbial Bacterioruberin: A Comprehensive Review
Mouliraj Palanisamy, Sathishkumar Ramalingam
Indian Journal of Microbiology.2024; 64(4): 1477. CrossRef - Congregibacter variabilis sp. nov. and Congregibacter brevis sp. nov. Within the OM60/NOR5 Clade, Isolated from Seawater, and Emended Description of the Genus Congregibacter
Hyeonsu Tak, Miri S. Park, Hyerim Cho, Yeonjung Lim, Jang-Cheon Cho
Journal of Microbiology.2024; 62(9): 739. CrossRef - Genomic insights on carotenoid synthesis by extremely halophilic archaea Haloarcula rubripromontorii BS2, Haloferax lucentense BBK2 and Halogeometricum borinquense E3 isolated from the solar salterns of India
Devika. N. Nagar, Kabilan Mani, Judith M. Braganca
Scientific Reports.2024;[Epub] CrossRef - Microbial bacterioruberin: The new C50 carotenoid player in food industries
Cassamo U. Mussagy, Angie V. Caicedo-Paz, Fabiane O. Farias, Leonardo M. de Souza Mesquita, Daniele Giuffrida, Laurent Dufossé
Food Microbiology.2024; 124: 104623. CrossRef - Evaluation of Various Escherichia coli Strains for Enhanced Lycopene Production
Jun Ren, Junhao Shen, Thi Duc Thai, Min-gyun Kim, Seung Ho Lee, Wonseop Lim, Dokyun Na
Journal of Microbiology and Biotechnology.2023; 33(7): 973. CrossRef - Bioactive molecules from haloarchaea: Scope and prospects for industrial and therapeutic applications
Jamseel Moopantakath, Madangchanok Imchen, V. T. Anju, Siddhardha Busi, Madhu Dyavaiah, Rosa María Martínez-Espinosa, Ranjith Kumavath
Frontiers in Microbiology.2023;[Epub] CrossRef
- Paenibacillus psychroresistens sp. nov., isolated from the soil of an Arctic glacial retreat
-
In-Tae Cha , Eui-Sang Cho , Yoo Kyung Lee , Seong Woon Roh , Myung-Ji Seo
-
J. Microbiol. 2019;57(7):569-574. Published online June 27, 2019
-
DOI: https://doi.org/10.1007/s12275-019-8666-x
-
-
1,038
View
-
0
Download
-
4
Web of Science
-
4
Crossref
-
Abstract
PDF
-
Strain ML311-T8T was isolated from a glacial retreat area in
Svalbard, Norway, and was taxonomically characterized by
a polyphasic approach. Upon phylogenetic analysis, strain
ML311-T8T was clustered with Paenibacillus arcticus MME2_
R6T and P. contaminans CKOBP-6T with 98.3–98.6 and 93.5–
93.9% 16S rRNA gene sequence similarities, respectively.
DNA-DNA hybridization values between strain ML311-T8T
and P. arcticus MME2_R6T was 19.9%. The genomic DNA
G+C content was 41.1 mol%. The isolated strain was Gramstain-
positive, strictly aerobic and rod-shaped, and grew in
0–0.5% (w/v) NaCl, at 4–23°C and pH 6.0–10.0, with optimal
growth in 0% (w/v) NaCl, at 20°C and pH 7.0–8.0. The predominant
respiratory quinone of strain ML311-T8T was MK-
7 and the major fatty acids were anteiso-C15:0 and C16:0. The
polar lipids of strain ML311-T8T were phosphatidylglycerol,
phosphatidylethanolamine, diphosphatidylglycerol, three unidentified
amino lipids, and three unidentified lipids. On the
basis of polyphasic taxonomic analysis, the strain ML311-T8T
is proposed to represent a novel species of the genus Paenibacillus,
for which the name Paenibacillus psychroresistens sp.
nov. is proposed. The type strain is ML311-T8T (= KCCM
43190T = JCM 31243T).
-
Citations
Citations to this article as recorded by - Microbiodiversity Landscape Present in the Mine-Tailings of the “Sierra de Huautla” Biosphere Reserve, Mexico
Maikel Gilberto Fernández-López, Ayixon Sánchez-Reyes, Marcos Eduardo Rosas-Ramírez, Edgar Balcázar-López
Water, Air, & Soil Pollution.2024;[Epub] CrossRef -
Paenibacillus dendrobii sp. nov., an indole-3-acetic acid-producing endophytic bacterium isolated from Dendrobium nobile
Yadong Hu, Hongjie Li, Yaoyi Chen, Qiling Zhang, Shigang Zheng, Dan Rao, Ze Chun, Ruoxi Zhao
International Journal of Systematic and Evolutionary Microbiology
.2023;[Epub] CrossRef - Validation List no. 206. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology
.2022;[Epub] CrossRef - Description of Paenibacillus dokdonensis sp. nov., a new bacterium isolated from soil
Jayoung Paek, Lu Bai, Yeseul Shin, Hongik Kim, Joong-Ki Kook, Young-Hyo Chang
International Journal of Systematic and Evolutionary Microbiology
.2019;[Epub] CrossRef
- Zunongwangia flava sp. nov., belonging to the family Flavobacteriaceae, isolated from Salicornia europaea
-
Eui-Sang Cho , In-Tae Cha , Hak-Jong Choi , Seong Woon Roh , Young-Do Nam , Sung Man Seo , Myung-Ji Seo
-
J. Microbiol. 2018;56(12):868-873. Published online October 25, 2018
-
DOI: https://doi.org/10.1007/s12275-018-8231-z
-
-
968
View
-
0
Download
-
6
Web of Science
-
6
Crossref
-
Abstract
PDF
-
A yellow pigmented bacterium designated strain MBLN094T
within the family Flavobacteriaceae was isolated from a halophyte
Salicornia europaea on the coast of the Yellow Sea.
This strain was a Gram-stain negative, aerobic, non-spore
forming, rod-shaped bacterium. Phylogenetic analysis of
the 16S rRNA gene sequence of strain MBLN094T was found
to be related to the genus Zunongwangia, exhibiting 16S rRNA
gene sequence similarity values of 97.0, 96.8, 96.4, and 96.3%
to Zunongwangia mangrovi P2E16T, Z. profunda SM-A87T,
Z. atlantica 22II14-10F7T, and Z. endophytica CPA58T, respectively.
Strain MBLN094T grew at 20‒37°C (optimum, 25‒
30°C), at pH 6.0‒10.0 (optimum, 7.0‒8.0), and with 0.5‒15.0%
(w/v) NaCl (optimum, 2.0‒5.0%). Menaquinone MK-6 was
the sole respiratory quinone. The polar lipids were phosphatidylethanolamine,
two unidentified aminolipids, and four
unidentified lipids. Major fatty acids were iso-C17:0 3-OH,
summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), and iso-C15:0.
The genomic DNA G + C content was 37.4 mol%. Based on
these polyphasic taxonomic data, strain MBLN094T is considered
to represent a novel species of the genus Zunongwangia,
for which the name Zunongwangia flava sp. nov. is
proposed. The type strain is MBLN094T (= KCTC 62279T =
JCM 32262T).
-
Citations
Citations to this article as recorded by - Sustainable agricultural management of saline soils in arid and semi-arid Mediterranean regions through halophytes, microbial and soil-based technologies
Salvadora Navarro-Torre, Pedro Garcia-Caparrós, Amaia Nogales, Maria Manuela Abreu, Erika Santos, Ana Lúcia Cortinhas, Ana Delaunay Caperta
Environmental and Experimental Botany.2023; 212: 105397. CrossRef -
Winogradskyella vincentii sp. nov. and Winogradskyella alexanderae sp. nov., two novel bacteria isolated from intertidal sediment
Yu-Yan Yue, Yu-Qi Ye, Zi-Yang Zhou, Meng-Di Zhang, Ya-Wei Jia, Zong-Jun Du
International Journal of Systematic and Evolutionary Microbiology
.2023;[Epub] CrossRef - Validation List no. 206. Valid publication of new names and new combinations effectively published outside the IJSEM
Aharon Oren, George M. Garrity
International Journal of Systematic and Evolutionary Microbiology
.2022;[Epub] CrossRef -
Zunongwangia pacifica sp. nov., isolated from surface seawater of the Western Pacific Ocean
Yongqing Chen, Tianqi Liu, Qiliang Lai, Chunming Dong, Zongze Shao
International Journal of Systematic and Evolutionary Microbiology
.2022;[Epub] CrossRef - Jejubacter calystegiae gen. nov., sp. nov., moderately halophilic, a new member of the family Enterobacteriaceae, isolated from beach morning glory
Lingmin Jiang, Dexin Wang, Jung-Sook Lee, Dae-Hyuk Kim, Jae Cheol Jeong, Cha Young Kim, Suk Weon Kim, Jiyoung Lee
Journal of Microbiology.2020; 58(5): 357. CrossRef - Isolation and genome analysis of Winogradskyella algicola sp. nov., the dominant bacterial species associated with the green alga Dunaliella tertiolecta
Jaeho Song, Yeonjung Lim, Hye-Jin Jang, Yochan Joung, Ilnam Kang, Seong-Joo Hong, Choul-Gyun Lee, Jang-Cheon Cho
Journal of Microbiology.2019; 57(11): 982. CrossRef
|
MSK
