- Altered Protein Expression Patterns of Mycobacterium tuberculosis Induced by ATB107
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Hongbo Shen , Enzhuo Yang , Feifei Wang , Ruiliang Jin , Shengfeng Xu , Qiang Huang , Honghai Wang
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J. Microbiol. 2010;48(3):337-346. Published online June 23, 2010
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DOI: https://doi.org/10.1007/s12275-010-9315-6
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ATB107 is a potent inhibitor of indole-3-glycerol phosphate synthase (IGPS). It can effectively inhibit the growth of clinical isolates of drug-resistant Mycobacterium tuberculosis strains as well as M. tuberculosis H37Rv. To investigate the mechanism of ATB107 action in M. tuberculosis, two-dimensional gel electrophoresis coupled with MALDI-TOF-MS analysis (2-DE-MS) was performed to illustrate alterations in the protein expression profile in response to ATB107. Results show that ATB107 affected tryptophan biosynthesis by decreasing the expression of protein encoded by Rv3246c, the transcriptional regulatory protein of MtrA belonging to the MtrA-MtrB two-component regulatory system, in both drug-sensitive and drug-resistant virulent strains. ATB107 might present a stress condition similar to isoniazid (INH) or ethionamide for M. tuberculosis since the altered expression in response to ATB107 of some genes, such as Rv3140, Rv2243, and Rv2428, is consistent with INH or ethionamide treatment. After incubation with
ATB107, the expression of 2 proteins encoded by Rv0685 and Rv2624c was down-regulated while that of protein encoded by Rv3140 was up-regulated in all M. tuberculosis strains used in this study. This may be the common response to tryptophan absence; however, relations to ATB107 are unknown and further evaluation is warranted.
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Citations
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Infectious Disorders - Drug Targets .2019; 19(1): 73. CrossRef - Systematic review on the proteomic profile of Mycobacterium tuberculosis exposed to drugs
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Paula AZ Campanerut-Sá, Luciana D Ghiraldi-Lopes, Jean E Meneguello, Adriana Fiorini, Geisa PC Evaristo, Vera LD Siqueira, Regiane BL Scodro, Eliana V Patussi, Lucélia Donatti, Emanuel M Souza, Rosilene F Cardoso
Future Microbiology.2016; 11(9): 1123. CrossRef - The Role of Amino Acid Permeases and Tryptophan Biosynthesis in Cryptococcus neoformans Survival
João Daniel Santos Fernandes, Kevin Martho, Veridiana Tofik, Marcelo A. Vallim, Renata C. Pascon, Yong-Sun Bahn
PLOS ONE.2015; 10(7): e0132369. CrossRef - Loop‐loop interactions govern multiple steps in indole‐3‐glycerol phosphate synthase catalysis
Margot J. Zaccardi, Kathleen F. O'Rourke, Eric M. Yezdimer, Laura J. Loggia, Svenja Woldt, David D. Boehr
Protein Science.2014; 23(3): 302. CrossRef - Functional Identification of the General Acid and Base in the Dehydration Step of Indole-3-glycerol Phosphate Synthase Catalysis
Margot J. Zaccardi, Eric M. Yezdimer, David D. Boehr
Journal of Biological Chemistry.2013; 288(37): 26350. CrossRef
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