- Purification and Characterization of the α-Glucosidase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI 756
-
Ana Flávia Azevedo Carvalho , Maurício Boscolo , Roberto da Silva , Henrique Ferreira , Eleni Gomes
-
J. Microbiol. 2010;48(4):452-459. Published online August 20, 2010
-
DOI: https://doi.org/10.1007/s12275-010-9319-2
-
-
706
View
-
0
Download
-
9
Crossref
-
 Abstract
 PDF
-
Αn α-glucosidase enzyme produced by the fungus Thermoascus aurantiacus CBMAI 756 was purified by ultra filtration, ammonium sulphate precipitation, and chromatography using Q Sepharose, Sephacryl S-200, and Superose 12 columns. The apparent molecular mass of the enzyme was 83 kDa as determined in gel electrophoresis. Maximum activity was observed at pH 4.5 at 70°C. Enzyme showed stability stable in the pH range of 3.0-9.0 and lost 40% of its initial activity at the temperatures of 40, 50, and 60°C. In the presence of ions Na+, Ba2+, Co2+, Ni2+, Mg2+, Mn2+, Al3+, Zn2+, Ca2+ this enzyme maintained 90-105% of its maximum activity and was inhibited by Cr3+, Ag+, and Hg2+. The enzyme showed a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides and α-PNPG. The Km measured for the α-glucosidase was 0.07 μM, with a Vmax of 318.0 μmol/min/mg.
-
Citations
Citations to this article as recorded by  - Biotechnological Potential of a Glucose-Tolerant β-Glucosidase from Rasamsonia composticola (β-GluRc) in Sugarcane Bagasse Saccharification
Isabela Pavão Vargas, Rodrigo Mattos Silva Galeano, Aline Pereira de Almeida, Ana Lorena de Oliveira Simas, João Victor Souza Ribeiro, Maria de Lourdes Teixeira de Moraes Polizeli, Douglas Chodi Masui, Giovana Cristina Giannesi, Roberto Ruller, Fabiana Fo
Waste and Biomass Valorization.2026; 17(8): 4723. CrossRef - Enzymatic characterization of the lignocellulolytic secretome of Penicillium Variabile wild strain from post-milling sugarcane bagasse in submerged fermentation
Janete Maria da Silva Alves, Renata Cândida Pinto Guerra, Maria Lúcia Gomes e Souza, Junio Cota Silva, Márcio Antônio Silva Pimenta, Maria Carolina Soares Lopes, Henrique Maia Valerio
Evidência.2026; 26: e36808. CrossRef - Enhanced Production, Purification, and Characterization of α-Glucosidase from NTG-Mutagenized Aspergillus niger for Industrial Applications
Bowei Yao, Qian Liu, Junjie Xiong, Guangming Feng, Zhongyi Chang, Hongliang Gao
Catalysts.2025; 15(5): 450. CrossRef - Purification, characterization of a novel α-glucosidase from Debaryomyces hansenii strain MCC 0202 and chromatographic separation for high purity isomalto-oligosaccharides production
Saravanan Rengarajan, Rameshthangam Palanivel
Process Biochemistry.2024; 136: 109. CrossRef - Chemical Constituents, Antioxidant, and α-Glucosidase Inhibitory Activities of Different Fermented Gynostemma Pentaphyllum Leaves and Untargeted Metabolomic Measurement of the Metabolite Variation
Xuechun Zhang, Shi Li, Zhibin Zhang, Kin Weng Kong, Zhenxing Wang, Xiahong He
Antioxidants.2023; 12(8): 1505. CrossRef - The development of a heterologous gene expression system in thermophilic fungus Thermoascus aurantiacus
Shenglin Hu, Zhefan Wang, Dongmei Wang, Jichao Wang, Jiong Hong
3 Biotech.2021;[Epub] CrossRef - Production and Catalytic Properties of Amylases fromLichtheimia ramosaandThermoascus aurantiacusby Solid-State Fermentation
Ana Paula Aguero de Oliveira, Maria Alice Silvestre, Nayara Fernanda Lisboa Garcia, Heloíza Ferreira Alves-Prado, André Rodrigues, Marcelo Fossa da Paz, Gustavo Graciano Fonseca, Rodrigo Simões Ribeiro Leite
The Scientific World Journal.2016; 2016: 1. CrossRef - Purification and characterization of a novel α-glucosidase from Malbranchea cinnamomea
Q. J. Yan, P. Han, S. Q. Yang, Z. Q. Jiang
Biotechnology Letters.2015; 37(6): 1279. CrossRef - Biochemical properties of an extracellular trehalase from Malbranchea pulchella var. Sulfurea
Marita Gimenez Pereira, Luis Henrique Souza Guimarães, Rosa Prazeres Melo Furriel, Maria de Lourdes Teixeira de Moraes Polizeli, Hector Francisco Terenzi, João Atílio Jorge
The Journal of Microbiology.2011; 49(5): 809. CrossRef
- Production, Partial Characterization, and Immobilization in Alginate Beads of an Alkaline Protease from a New Thermophilic Fungus Myceliophthora sp.
-
Letícia Maria Zanphorlin , Fernanda Dell Antonio Facchini , Filipe Vasconcelos , Rafaella Costa Bonugli-Santos , André Rodrigues , Lara Durães Sette , Eleni Gomes , Gustavo Orlando Bonilla-Rodriguez
-
J. Microbiol. 2010;48(3):331-336. Published online June 23, 2010
-
DOI: https://doi.org/10.1007/s12275-010-9269-8
-
-
793
View
-
0
Download
-
25
Crossref
-
Abstract
PDF
-
Thermophilic fungi produce thermostable enzymes which have a number of applications, mainly in biotechnological processes. In this work, we describe the characterization of a protease produced in solidstate (SSF) and submerged (SmF) fermentations by a newly isolated thermophilic fungus identified as a putative new species in the genus Myceliophthora. Enzyme-production rate was evaluated for both fermentation processes, and in SSF, using a medium composed of a mixture of wheat bran and casein, the proteolytic output was 4.5-fold larger than that obtained in SmF. Additionally, the peak of proteolytic activity was obtained after 3 days for SSF whereas for SmF it was after 4 days. The crude enzyme obtained by both
SSF and SmF displayed similar optimum temperature at 50°C, but the optimum pH shifted from 7 (SmF) to 9 (SSF). The alkaline protease produced through solid-state fermentation (SSF), was immobilized on beads of calcium alginate, allowing comparative analyses of free and immobilized proteases to be carried out. It was
observed that both optimum temperature and thermal stability of the immobilized enzyme were higher than for the free enzyme. Moreover, the immobilized enzyme showed considerable stability for up to 7 reuses.
-
Citations
Citations to this article as recorded by - Production and Potential Application of an Alkaline Serine Peptidase from Myceliophtora heterothallica for Biofilm Removal
Rafael Amadeu Barreto, Emanuella Roberto Ribeiro, Cíntia Lionela Ambrósio de Menezes, Mohammed Anas Zaiter, Maurício Boscolo, Roberto da Silva, Eleni Gomes, Ronivaldo Rodrigues da Silva
Current Microbiology.2025;[Epub] CrossRef - Purification and Characterization of Alkaline Protease Isolated from Cotton (Gossypium hirsutum) Seeds
Asghar Ali Shaikh, Muhammad Umer Dahot, Abdul Sajid, Syed Habib Ahmed Naqvi
Journal of Applied Research in Plant Sciences.2024; 5(01): 34. CrossRef - Heterologous expression of GH11 xylanase from Myceliophthora heterothallica F.2.1.4 in Pichia pastoris
Gabriela Salvador de Amo, Carolina Bezerra-Bussoli, Ronivaldo Rodrigues da Silva, Luciano Takeshi Kishi, Henrique Ferreira, Eleni Gomes, Gustavo Orlando Bonilla-Rodriguez
Biocatalysis and Agricultural Biotechnology.2024; 61: 103343. CrossRef - Production, optimization, and purification of alkaline thermotolerant protease from newly isolated Phalaris minor seeds
Umber Zaman, Khalil ur Rehman, Shahid Ullah Khan, Syed Badshah, Khaled M. Hosny, Majd A. Alghamdi, Hatem K. Hmid, Mohammed Alissa, Deena M. Bukhary, Ehab A. Abdelrahman
International Journal of Biological Macromolecules.2023; 233: 123544. CrossRef - Biocatalytic Profiling of Free and Immobilized Partially Purified Alkaline Protease from an Autochthonous Bacillus aryabhattai Ab15-ES
Adegoke Isiaka Adetunji, Ademola Olufolahan Olaniran
Reactions.2023; 4(2): 231. CrossRef - Fungal alkaline proteases and their potential applications in different industries
Kadambari Subhash Pawar, Paras Nath Singh, Sanjay Kumar Singh
Frontiers in Microbiology.2023;[Epub] CrossRef - Valorization of agricultural residues using Myceliophthora thermophila as a platform for production of lignocellulolytic enzymes for cellulose saccharification
Nathália Gonsales da Rosa-Garzon, Hélen Julie Laure, José César Rosa, Hamilton Cabral
Biomass and Bioenergy.2022; 161: 106452. CrossRef - Immobilized fungal enzymes: Innovations and potential applications in biodegradation and biosynthesis
Yifan Gao, Kshitjia Shah, Ivy Kwok, Meng Wang, Leonard H. Rome, Shaily Mahendra
Biotechnology Advances.2022; 57: 107936. CrossRef - An Overview of Proteases: Production, Downstream Processes and Industrial Applications
Nathiele Contrera Gimenes, Edgar Silveira, Elias Basile Tambourgi
Separation & Purification Reviews.2021; 50(3): 223. CrossRef - Optimization of protease production from Bacillus halodurans under solid state fermentation using agrowastes
Chellapandian Balachandran, Alagumalai Vishali, Natarajan Arun Nagendran, Kathirvelu Baskar, Abeer Hashem, Elsayed Fathi Abd_Allah
Saudi Journal of Biological Sciences.2021; 28(8): 4263. CrossRef - Bioassay guided fractionation of bioactive metabolite from Corynascus verrucosus inhabiting Croton bonplandianus Baill
N. Chandra Mohana, D. Rakshith, H.C. Yashavantha Rao, K.P. Ramesha, B.R. Nuthan, S. Satish
Process Biochemistry.2020; 98: 106. CrossRef - Characterization and immobilization of protease secreted by the fungus Moorella speciosa
Juliana Mota de Oliveira, Pedro Fernandes, Raquel Guimarães Benevides, Sandra Aparecida de Assis
3 Biotech.2020;[Epub] CrossRef - Protease Production from Cheotomium globusum Through Central Composite Design Using Agricultural Wastes and Its Immobilization for Industrial Exploitation
Fareeha Nadeem, Tahir Mehmood, Muhammad Naveed, Shazia Shamas, Tasmia Saman, Zahid Anwar
Waste and Biomass Valorization.2020; 11(12): 6529. CrossRef - Heterologous expression, purification and biochemical characterization of a new xylanase from Myceliophthora heterothallica F.2.1.4
Gabriela Salvador de Amo, Carolina Bezerra-Bussoli, Ronivaldo Rodrigues da Silva, Luciano Takeshi Kishi, Henrique Ferreira, Ricardo Barros Mariutti, Raghuvir Krishnaswamy Arni, Eleni Gomes, Gustavo Orlando Bonilla-Rodriguez
International Journal of Biological Macromolecules.2019; 131: 798. CrossRef - Purification and Physicochemical Characterization of a Novel Thermostable Xylanase Secreted by the Fungus Myceliophthora heterothallica F.2.1.4
Lorena Caixeta de Oliveira Simões, Ronivaldo Rodrigues da Silva, Carlos Eduardo de Oliveira Nascimento, Maurício Boscolo, Eleni Gomes, Roberto da Silva
Applied Biochemistry and Biotechnology.2019; 188(4): 991. CrossRef - Study on immobilization of papain with magnetic porous alginate microspheres
Hui Wang, Dongxu Shao
IOP Conference Series: Materials Science and Engineering.2018; 397: 012031. CrossRef - Partitioning and extraction protease from Aspergillus tamarii URM4634 using PEG-citrate aqueous two-phase systems
Osmar Soares da Silva, Matheus Henrique Gouveia Gomes, Rodrigo Lira de Oliveira, Ana Lúcia Figueiredo Porto, Attilio Converti, Tatiana Souza Porto
Biocatalysis and Agricultural Biotechnology.2017; 9: 168. CrossRef - Biochemical properties of a serine protease fromAspergillus flavusand application in dehairing
Daniel Guerra Franco, Regiane Nogueira Spalanzani, Emmly Ernesto Lima, Clarice Rossato Marchetti, Patrícia Oliveira Silva, Douglas Chodi Masui, Giovana Cristina Giannesi, Fabiana Fonseca Zanoelo
Biocatalysis and Biotransformation.2017; 35(4): 249. CrossRef - Bioprocess optimization for production of thermoalkali-stable protease from Bacillus subtilis K-1 under solid-state fermentation
Satbir Singh, Bijender Kumar Bajaj
Preparative Biochemistry & Biotechnology.2016; 46(7): 717. CrossRef - Purification and characterization of an extracellular haloalkaline serine protease from the moderately halophilic bacterium, Bacillus iranensis (X5B)
Hossein Ghafoori, Mansoure Askari, Sajjad Sarikhan
Extremophiles.2016; 20(2): 115. CrossRef - Novel Protease from <i>Aspergillus tamarii</i> URM4634: Production and Characterization Using Inexpensive Agroindustrial Substrates by Solid-State Fermentation
Osmar Soares da Silva, Rodrigo Lira de Oliveira, Cristina Maria Souza-Motta, Ana Lúcia Figueiredo Porto, Tatiana Souza Porto
Advances in Enzyme Research.2016; 04(04): 125. CrossRef - Production, optimization and partial purification of protease fromBacillus subtilis
Gaurav Pant, Anil Prakash, J.V.P. Pavani, Sayantan Bera, G.V.N.S. Deviram, Ajay Kumar, Mitali Panchpuri, Ravi Gyana Prasuna
Journal of Taibah University for Science.2015; 9(1): 50. CrossRef - Production and Characterization of New Fibrinolytic Protease from <i>Mucor subtillissimus</i> UCP 1262 in Solid-State Fermentation
Thiago Pajeú Nascimento, Amanda Emmanuelle Sales, Camila Souza Porto, Romero Marcos Pedrosa Brandão, Galba Maria Campos Takaki, Jose Antônio Couto Teixeira, Tatiana Souza Porto, Ana Lúcia Figueiredo Porto
Advances in Enzyme Research.2015; 03(03): 81. CrossRef - Immobilization of halophilic Bacillus sp. EMB9 protease on functionalized silica nanoparticles and application in whey protein hydrolysis
Rajeshwari Sinha, S. K. Khare
Bioprocess and Biosystems Engineering.2015; 38(4): 739. CrossRef - Purification and characterization of a new alkaline serine protease from the thermophilic fungus Myceliophthora sp.
L.M. Zanphorlin, H. Cabral, E. Arantes, D. Assis, L. Juliano, M.A. Juliano, R. Da-Silva, E. Gomes, G.O. Bonilla-Rodriguez
Process Biochemistry.2011; 46(11): 2137. CrossRef
- A Specific Short Dextrin-Hydrolyzing Extracellular Glucosidase from the Thermophilic Fungus Thermoascus aurantiacus 179-5
-
Ana Flavia Azevedo Carvalho , Aline Zorzetto Gonclves , Roberto da Silva , Eleni Gomes
-
J. Microbiol. 2006;44(3):276-283.
-
DOI: https://doi.org/2385 [pii]
-
-
Abstract
PDF
-
The thermophilic fungus Thermoascus aurantiacus 179-5 produced large quantities of a glucosidase which preferentially hydrolyzed maltose over starch. Enzyme production was high in submerged fermentation, with a maximal activity of 30 U/ml after 336 h of fermentation. In solid-state fermentation, the activity of the enzyme was 22 U/ml at 144 h in medium containing wheat bran and 5.8 U/ml at 48 h when cassava pulp was used as the culture medium. The enzyme was specific for maltose, very slowly hydrolyzed starch, dextrins (2-7G) and the synthetic substrate (α-PNPG), and did not hydrolyze sucrose. These properties suggest that the enzyme is a type II α-glucosidase. The optimum temperature of the enzyme was 70?. In addition, the enzyme was highly thermostable (100% stability for 10 h at 60? and a half-life of 15 min at 80?), and stable within a wide pH range.
- Production of Saccharogenic and Dextrinogenic Amylases by Rhizomucor pusillus A 13.36
-
Tony M. Silva , Derlene Attili-Angelis , Ana Flavia Azevedo Carvalho , Roberto Da Silva , Mauricio Boscolo , Eleni Gomes
-
J. Microbiol. 2005;43(6):561-568.
-
DOI: https://doi.org/2289 [pii]
-
-
Abstract
PDF
-
A newly-isolated thermophilic strain of the zygomycete fungus Rhizomucor pusillus 13.36 produced highly active dextrinogenic and saccharogenic enzymes. Cassava pulp was a good alternative substrate for amylase production. Dextrinogenic and saccharogenic amylases exhibited optimum activities at a pH of 4.0-4.5 and 5.0 respectively and at a temperature of 75oC. The enzymes were highly thermostable, with no detectable loss of saccharogenic or dextrinogenic activity after 1 h and 6 h at 60oC, respectively. The saccharogenic activity was inhibited by Ca2+ while the dextrinogenic was indifferent to this ion. Both activities were inhibited by Fe2+ and Cu2+ Hydrolysis of soluble starch by the crude enzyme yielded 66% glucose, 19.5% maltose, 7.7% maltotriose and 6.6% oligosaccharides.
|
MSK
