Journal of Microbiology

Duraisamy Senthil Raja

2 Articles

Spectral characterization of a pteridine derivative from cyanide-utilizing bacterium Bacillus subtilis - JN989651: S. Durairaju Nisshanthini , Antony K. Teresa Infanta S. , Duraisamy Senthil Raja , Karuppannan Natarajan , M. Palaniswamy , Jayaraman Angayarkanni; J. Microbiol. 2015;53(4):262-271. Published online March 4, 2015; DOI: https://doi.org/10.1007/s12275-015-4138-0

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Soil and water samples were collected from various regions of SIPCOT and nearby Vanappadi Lake, Ranipet, Tamilnadu, India. Based on their colony morphology and their stability during subculturing, 72 bacteria were isolated, of which 14 isolates were actinomycetes. Preliminary selection was carried out to exploit the ability of the microorganisms to utilize sodium cyanate as nitrogen source. Those organisms that were able to utilize cyanate were subjected to secondary screening viz., utilization of sodium cyanide as the nitrogen source. The oxygenolytic cleavage of cyanide is dependent on cyanide monooxygenase which obligately requires pterin cofactor for its activity. Based on this, the organisms capable of utilizing sodium cyanide were tested for the presence of pterin. Thin layer chromatography (TLC) of the cell extracts using n-butanol: 5 N glacial acetic acid (4:1) revealed that 10 out of 12 organisms that were able to utilize cyanide had the pterin-related blue fluorescent compound in the cell extract. The cell extracts of these 10 organisms were subjected to high performance thin layer chromatography (HPTLC) for further confirmation using a pterin standard. Based on the incubation period, cell biomass yield, peak height and area, strain VPW3 was selected and was identified as Bacillus subtilis. The Rf value of the cell extract was 0.73 which was consistent with the 0.74 Rf value of the pterin standard when scanned at 254 nm. The compound was extracted and purified by preparative High Performance Liquid Chromatography (HPLC). Characterization of the compound was performed by ultraviolet spectrum, fluorescence spectrum, Electrospray Ionization-Mass Spectrometry (ESI-MS), and Nuclear Magnetic Resonance spectroscopy (NMR). The compound is proposed to be 6-propionyl pterin (2-amino-6- propionyl-3H-pteridin-4-one).

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BS Sabna, Ramasamy Mahendran, Jeyakumar Balakrishnan, Chih-Yang Huang, PJ Jerimon, Anto Thomas, Raju Eswaran, P Naveen, Angayarkanni Jayaraman
Probiotics and Antimicrobial Proteins.2026; 18(2): 1775. CrossRef
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Anita Gupta, Saroj Kanta Barik, Pankaj Kumar Srivastava
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Proceedings of the National Academy of Sciences.2025;[Epub] CrossRef
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César Julio Cáceda Quiroz, Gabriela de Lourdes Fora Quispe, Milena Carpio Mamani, Gisela July Maraza Choque, Elisban Juani Sacari Sacari
Water.2023; 15(20): 3645. CrossRef
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Narges Malmir, Najaf Allahyari Fard, Saeed Aminzadeh, Zahra Moghaddassi-Jahromi, Lukhanyo Mekuto
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H. D. Douglas, I. V. Ermakov, W. Gellermann
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Ramasamy Mahendran, Sabna BS, Murugesan Thandeeswaran, Kiran kG, Muthu Vijayasarathy, Jayaraman Angayarkanni, Gayathri Muthusamy
Current Microbiology.2020; 77(4): 578. CrossRef
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Melissa A. Merrill Floyd, Amy J. Williams, Andrej Grubisic, David Emerson
Astrobiology.2019; 19(1): 40. CrossRef
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Murugesan Thandeeswaran, Sajitha Bijukumar, Mani Arulkumar, Ramasamy Mahendran, Muthusamy Palaniswamy, Jayaraman Angayarkanni
Biotechnology Research and Innovation.2019; 3(1): 159. CrossRef
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Ramasamy Mahendran, Murugesan Thandeeswaran, Gopikrishnan Kiran, Mani Arulkumar, K. A. Ayub Nawaz, Jayamanoharan Jabastin, Balraj Janani, Thomas Anto Thomas, Jayaraman Angayarkanni
Current Microbiology.2018; 75(6): 684. CrossRef
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Sanjay S. Limaye, Rakesh Mogul, David J. Smith, Arif H. Ansari, Grzegorz P. Słowik, Parag Vaishampayan
Astrobiology.2018; 18(9): 1181. CrossRef
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Thandeeswaran Murugesan, Nisshanthini Durairaj, Mahendran Ramasamy, Karunya Jayaraman, Muthusamy Palaniswamy, Angayarkanni Jayaraman
Applied Microbiology and Biotechnology.2018; 102(4): 1557. CrossRef
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Assessment of Resistomycin, as an Anticancer Compound Isolated and Characterized from Streptomyces aurantiacus AAA5: Rajendran Vijayabharathi , Per Bruheim , Trygve Andreassen , Duraisamy Senthil Raja , Palanisamy Bruntha Devi , Sathyaseelan Sathyabama , Venkatesan Brindha Priyadarisini; J. Microbiol. 2011;49(6):920-926. Published online December 28, 2011; DOI: https://doi.org/10.1007/s12275-011-1260-5

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Abstract PDF

A new actinomycete strain, isolated from humus soils in the Western Ghats, was found to be an efficient pigment producer. The strain, designated AAA5, was identified as a putative Streptomyces aurantiacus strain based on cultural properties, morphology, carbon source utilization, and analysis of the 16S rRNA gene. The strain produced a reddish-brown pigmented compound during the secondary metabolites phase. A yellow compound was derived from the extracted pigment and was identified as the quinone-related antibiotic resistomycin based on ultraviolet–visible spectrophotometry, fourier transform infrared spectroscopy, liquid chromatography and mass spectroscopy, and nuclear magnetic resonance analyses. The AAA5 strain was found to produce large quantities of resistomycin (52.5 mg/L). It showed potent cytotoxic activity against cell lines viz. HepG2 (hepatic carcinoma) and HeLa (cervical carcinoma) in vitro, with growth inhibition (GI50) of 0.006 and 0.005 μg/ml, respectively. The strain also exhibited broad antimicrobial activities against both Gram-positive and Gram-negative bacteria. Therefore, AAA5 may have great potential as an industrial resistomycin-producing strain.

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Anand Kumar Gaurav, Arpan Mukherjee, Tushar Goyal, Bansh Narayan Singh, Gowardhan Kumar Chouhan, Sanjay Guleria, Jay Prakash Verma
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Microbe-derived aromatic polyketides toward MRSA infection: current advances and perspectives
Fangfang Duan, Jiaying Lai, Linjie Wei, Siran Li, Polina Lopukhina, Zihuan Sang, Chen Chen, Xiaoyi Wei, Hongxin Liu, Haibo Tan
Natural Product Reports.2026; 43(4): 718. CrossRef
Revisiting heliomycin: Chemistry, biological activity, and perspectives for drug development
Galina I. Buravchenko, Alexander S. Tikhomirov, Andrey E. Shchekotikhin
European Journal of Medicinal Chemistry.2026; 316: 119060. CrossRef
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Mohamed S Abdelfattah, Ibrahim H Eissa, Ahmad E Mostafa, Eslam B Elkaeed, Aisha A Alsfouk, Abdulla A Mahmoud, Ibrahim M Ibrahim, Ahmed M Metwaly
Journal of Chemical Research.2025;[Epub] CrossRef
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Pakistan Journal of Biological Sciences.2025; 28(12): 749. CrossRef
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Geeta Chhetri, Myeong Ji Kim, Inhyup Kim, Duc V. H. Tran, Young-Woo Kim, Hyun Woo Kim, Taegun Seo
Frontiers in Microbiology.2024;[Epub] CrossRef
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М. Kharkhota, М. Kharchuk, А. Kharchuk, G. Grabova, Yu Noskov, R. Linnik, А. Makeiev, L. Avdieieva
Biochemical and Biophysical Research Communications.2024; 741: 151040. CrossRef
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Atikul Islam, Yu-Chun Chang, Xiao-Chi Chen, Chia-Wei Weng, Chien-Yu Chen, Che-Wei Wang, Mu-Kuan Chen, Alexander S Tikhomirov, Andrey E Shchekotikhin, Pin Ju Chueh
eLife.2024;[Epub] CrossRef
Streptomyces Paradigm in Anticancer Therapy: A State-of-the Art Review
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Current Cancer Therapy Reviews.2024; 20(4): 386. CrossRef
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Scientific Reports.2024;[Epub] CrossRef
Water-soluble 4-(dimethylaminomethyl)heliomycin exerts greater antitumor effects than parental heliomycin by targeting the tNOX-SIRT1 axis and apoptosis in oral cancer cells
Atikul Islam, Yu-Chun Chang, Xiao-Chi Chen, Chia-Wei Weng, Chien-Yu Chen, Che-Wei Wang, Mu-Kuan Chen, Alexander S Tikhomirov, Andrey E Shchekotikhin, Pin Ju Chueh
eLife.2024;[Epub] CrossRef
Resistomycin Suppresses Prostate Cancer Cell Growth by Instigating Oxidative Stress, Mitochondrial Apoptosis, and Cell Cycle Arrest
Abeer S. Aloufi, Ola A. Habotta, Mohamed S. Abdelfattah, Marina N. Habib, Mohamed M. Omran, Sally A. Ali, Ahmed E. Abdel Moneim, Shereen M. Korany, Aisha M. Alrajhi
Molecules.2023; 28(23): 7871. CrossRef
Resistomycin Inhibits Wnt/β-Catenin Signaling to Induce the Apoptotic Death of Human Colorectal Cancer Cells
Yaoyao Zhu, E Zhang, Huan Gao, Chuangeng Shang, Mengxiong Yin, Mingtao Ma, Yu Liu, Xuanfeng Zhang, Xia Li
Marine Drugs.2023; 21(12): 622. CrossRef
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S. Nair, J. Abraham
Applied Biochemistry and Microbiology.2022; 58(6): 701. CrossRef
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Zinal T. Vasava, Kruti J. Mistry, Pooja P. Patel, Anoop R. Markande
Acta Ecologica Sinica.2022; 42(6): 593. CrossRef
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Aixa A. Sarmiento-Tovar, Laura Silva, Jeysson Sánchez-Suárez, Luis Diaz
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International Nano Letters.2022; 12(4): 409. CrossRef
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Journal of Natural Products.2021; 84(5): 1617. CrossRef
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Zhuo Han, Xingming Zhao, E Zhang, Jiahui Ma, Hao Zhang, Jianjiang Li, Weidong Xie, Xia Li
Pharmaceuticals.2021; 14(10): 958. CrossRef
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Mohammed I. Y. Elmallah, Sheron Cogo, Andrei A. Constantinescu, Selene Elifio-Esposito, Mohammed S. Abdelfattah, Olivier Micheau
Cells.2020; 9(8): 1760. CrossRef
Isolation of Resistomycin from a Terrestrial Actinomycete with TRAIL Resistance-overcoming Activity
Xuefeidan Liu, Midori A. Arai, Kazufumi Toume and Masami Ishibashi
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Journal of Microbiology.2018; 56(10): 753. CrossRef
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Journal of Biomolecular Structure and Dynamics.2018; 36(9): 2361. CrossRef
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