Journal of Microbiology

Duk-Ju Hwang

4 Articles

Evaluation of Bakanae Disease Progression Caused by Fusarium fujikuroi in Oryza sativa L.: In Sun Hwang , Woo-Ri Kang , Duk-Ju Hwang , Shin-Chul Bae , Sung-Hwan Yun , Il-Pyung Ahn; J. Microbiol. 2013;51(6):858-865. Published online December 19, 2013; DOI: https://doi.org/10.1007/s12275-013-3472-3

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Bakanae disease caused by Fusarium fujikuroi is an important fungal disease in rice. Among the seven strains isolated from symptomatic rice grains in this study, one strain, FfB14, triggered severe root growth inhibition and decay in the crown and root of rice seedlings. The remaining six strains caused typical Bakanae symptoms such as etiolation and abnormal succulent rice growth. To reveal the relationship between mycelial growth in the infected tissues and Bakanae disease progression, we have established a reliable quantification
method
using real time PCR that employs a primer pair and dual-labeled probe specific to a unigene encoding F. fujikuroi PNG1 (FfPNG1), which is located upstream of the fumonisin biosynthesis gene cluster. Plotting the crossing point (CP) values from the infected tissue DNAs on a standard curve revealed the active fungal growth of FfB14 in the root and crown of rice seedlings, while the growth rate of FfB20 in rice was more than 4 times lower than FfB14. Massive infective mycelial growth of FfB14 was evident in rice stems and crown; however, FfB20 did not exhibit vigorous growth. Our quantitative evaluation system is applicable for the identification of fungal virulence factors other than gibberellin.

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Map-Based Cloning and Characterization of a Major QTL Gene, FfR1, Which Confers Resistance to Rice Bakanae Disease
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Differential biochemical response of basmati and non-basmati rice seeds upon bakanae (Fusarium fujikuroi) infection
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Genome-Wide Identification and Functional Analysis of the bZIP Transcription Factor Family in Rice Bakanae Disease Pathogen, Fusarium fujikuroi
Kehan Zhao, Lianmeng Liu, Shiwen Huang
International Journal of Molecular Sciences.2022; 23(12): 6658. CrossRef
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Anita Puyam, P. P. S. Pannu, Jaspal Kaur, Shikha Sethi, Kiranjot Kaur
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Younghae Choi, Boknam Jung, Taiying Li, Jungkwan Lee
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Development and Validation of a TaqMan Real-Time PCR Assay for the Specific Detection and Quantification ofFusarium fujikuroiin Rice Plants and Seeds
Greice Amaral Carneiro, Slavica Matić, Giuseppe Ortu, Angelo Garibaldi, Davide Spadaro, Maria Lodovica Gullino
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Comparative genomics of geographically distant Fusarium fujikuroi isolates revealed two distinct pathotypes correlating with secondary metabolite profiles
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Detecting Bakanae disease in rice seedlings by machine vision
Chia-Lin Chung, Kai-Jyun Huang, Szu-Yu Chen, Ming-Hsing Lai, Yu-Chia Chen, Yan-Fu Kuo
Computers and Electronics in Agriculture.2016; 121: 404. CrossRef
Multi-Homologous Recombination-Based Gene Manipulation in the Rice Pathogen Fusarium fujikuroi
In Sun Hwang, Il-Pyung Ahn
The Plant Pathology Journal.2016; 32(3): 173. CrossRef
The biosynthesis of gibberellic acids by the transformants of orchid-associated Fusarium oxysporum
Elena A. Tsavkelova
Mycological Progress.2016;[Epub] CrossRef
The Genetic Structure, Virulence, and Fungicide Sensitivity of Fusarium fujikuroi in Taiwan
Yu-Chia Chen, Ming-Hsin Lai, Chia-Yi Wu, Tsung-Chun Lin, An-Hsiu Cheng, Chin-Cheng Yang, Hsin-Yuh Wu, Sheng-Chi Chu, Chien-Chih Kuo, Yea-Fang Wu, Guo-Cih Lin, Min-Nan Tseng, Yi-Chen Tsai, Chun-Chi Lin, Chi-Yu Chen, Jenn-Wen Huang, Heng-An Lin, Chia-Lin Ch
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Comparison of the Antimicrobial Properties of Chitosan Oligosaccharides (COS) and EDTA against Fusarium fujikuroi Causing Rice Bakanae Disease
Seung Won Kim, Jae Kweon Park, Cho Heun Lee, Bum-Soo Hahn, Ja Choon Koo
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Jasmonic Acid, Abscisic Acid, and Salicylic Acid Are Involved in the Phytoalexin Responses of Rice to Fusarium fujikuroi, a High Gibberellin Producer Pathogen
Ilenia Siciliano, Greice Amaral Carneiro, Davide Spadaro, Angelo Garibaldi, Maria Lodovica Gullino
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Mapping of qBK1, a major QTL for bakanae disease resistance in rice
Yeon-Jae Hur, Sais Beul Lee, Tae Heon Kim, Tackmin Kwon, Jong-Hee Lee, Dong-Jin Shin, Soo-Kwon Park, Un-Ha Hwang, Jun Hyeon Cho, Young-Nam Yoon, Un-Sang Yeo, You-Chun Song, Do-Yeon Kwak, Min-Hee Nam, Dong-Soo Park
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Evaluation of Fusarium Head Blight in Barley Infected by Fusarium graminearum: Woo-Ri Kang , Duk-Ju Hwang , Shin-Chul Bae , Theresa Lee , Soonok Kim , Il-Pyung Ahn; J. Microbiol. 2013;51(4):540-543. Published online August 30, 2013; DOI: https://doi.org/10.1007/s12275-013-3338-8

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Abstract PDF

Fusarium head blight, which is primarily caused by Fusarium graminearum, is a devastating disease in the barley field. A real-time PCR protocol was developed to evaluate the growth of this pathogen in the host plant tissues. All four strains harbored the gene encoding ATP-BINDING CASSETTE TRANSPORTER (FgABC; FGSG_00541) as a single copy within their genomes. Our Southern blot result was identical with the genomic data for F. graminearum strain PH-1. Based on the crossing point (CP) values obtained in our TaqMan real-time PCR analysis, two standard curves describing the relationship among the CP value, FgABC copy number, and amount of fungal DNA were constructed. Chronological enumeration of fungal growth was coincided with the symptom development.

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Quantification of Rice Sheath Blight Progression Caused by Rhizoctonia solani: Mukhamad Su’udi , Jong-Mi Park , Woo-Ri Kang , Duk-Ju Hwang , Soonok Kim , Il-Pyung Ahn; J. Microbiol. 2013;51(3):380-388. Published online June 28, 2013; DOI: https://doi.org/10.1007/s12275-013-3274-7

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Abstract PDF

Rhizoctonia solani has a wide host range, including almost all cultivated crops and its subgroup anastomosis group (AG)-1 IA causes sheath blight in rice. An accurate measurement of pathogen’s biomass is a convincing tool for enumeration of this disease. Mycological characteristics and molecular diagnosis simultaneously supported that all six strains in this study were R. solani AG-1 IA. Heterokaryons between strains Rs40104, Rs40105, and Rs45811 were stable and viable, whereas Rs40103 and Rs40106 did not form viable fused cells, except for the combination of Rs40106 and Rs40104. A primer pair was highly specific to RsAROM gene of R. solani strains and the amplified fragment exists as double copies within fungal genome. The relationship between crossing point (CP) values and the amount of fungal DNA was reliable (R2>0.99). Based on these results, we determined R. solani’s proliferation within infected stems through real time PCR using a primer pair and a Taqman probe specific to the RsAROM gene. The amount of fungal DNA within the 250 ng of tissue DNA from rice cv. Dongjin infected with Rs40104, Rs40105, and Rs45811 were 7.436, 5.830, and 5.085 ng, respectively. In contrast, the fungal DNAs within the stems inoculated with Rs40103 and Rs40106 were 0.091 and 0.842 ng. The sheath blight symptom progression approximately coincided with the amount of fungal DNA within the symptoms. In summary, our quantitative evaluation method provided reliable and objective results reflecting the amount of fungal biomass within the infected tissues and would be useful for evaluation of resistance germplasm or fungicides and estimation of inoculum potential.

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Evaluation of bakanae disease progression caused by Fusarium fujikuroi in Oryza sativa L.
In Sun Hwang, Woo-Ri Kang, Duk-Ju Hwang, Shin-Chul Bae, Sung-Hwan Yun, Il-Pyung Ahn
Journal of Microbiology.2013; 51(6): 858. CrossRef
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Quantification of Rice Brown Leaf Spot through Taqman Real-Time PCR Specific to the Unigene Encoding Cochliobolus miyabeanus SCYTALONE DEHYDRATASE1 Involved in Fungal Melanin Biosynthesis: Mukhamad Su’udi , Jong-Mi Park , Woo-Ri Kang , Sang-Ryeol Park , Duk-Ju Hwang , Il-Pyung Ahn; J. Microbiol. 2012;50(6):947-954. Published online December 30, 2012; DOI: https://doi.org/10.1007/s12275-012-2538-y

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Rice brown leaf spot is a major disease in the rice paddy field. The causal agent Cochliobolus miyabeanus is an ascomycete fungus and a representative necrotrophic pathogen in the investigation of rice-microbe interactions. The aims of this research were to identify a quantitative evaluation method to determine the amount of C. miyabeanus proliferation in planta and determine the method’s sensitivity. Real-time polymerase chain reaction (PCR) was employed in combination with the primer pair and Taqman probe specific to CmSCD1, a C. miyabeanus unigene encoding SCYTALONE DEHYDRATASE, which is involved in fungal melanin biosynthesis. Comparative analysis of the nucleotide sequences of CmSCD1 from Korean strains with those from the Japanese and Taiwanese strains revealed some sequence differences. Based on the crossing point (CP) values from Taqman realtime PCR containing a series of increasing concentrations of cloned amplicon or fungal genomic DNA, linear regressions with a high level of reliability (R2>0.997) were constructed. This system was able to estimate fungal genomic DNA at the picogram level. The reliability of this equation was further confirmed using DNA samples from both resistant and susceptible cultivars infected with C. miyabeanus. In summary, our quantitative system is a powerful alternative in brown leaf spot forecasting and in the consistent evaluation of disease progression.

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Evaluation of bakanae disease progression caused by Fusarium fujikuroi in Oryza sativa L.
In Sun Hwang, Woo-Ri Kang, Duk-Ju Hwang, Shin-Chul Bae, Sung-Hwan Yun, Il-Pyung Ahn
Journal of Microbiology.2013; 51(6): 858. CrossRef

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