- Roseovarius tibetensis sp. nov., a halophilic bacterium isolated from Lake LongmuCo on Tibetan Plateau
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Hui-bin Lu , Xiao-feng Xue , Dorji Phurbu , Peng Xing , Qing-long Wu
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J. Microbiol. 2018;56(11):783-789. Published online October 24, 2018
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DOI: https://doi.org/10.1007/s12275-018-8178-0
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 Abstract
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Two Gram-stain negative halophilic strains, designated as
LM2T and LM4, were isolated from Lake LongmuCo on
Tibetan Plateau. These two strains were aerobic, catalaseand
oxidase- positive, nonmotile and rod-shaped organisms.
Phylogenetic analysis based on 16S rRNA gene sequences
indicated that LM2T and LM4 belong to the genus Roseovarius,
with Roseovarius tolerans EL-172T (97.3% and 97.4%
16S rRNA gene sequence similarity, respectively) and Roseovarius
azorensis SSW084T (95.5% and 95.6% 16S rRNA gene
sequence similarity, respectively) as their closest neighbors.
Q-10 was the sole respiratory quinone of these two strains.
The major fatty acids were C18:1 ω7c/C18:1 ω6c, C16:0, C19:0 cyclo
ω8c, and 11-methyl C18:1 ω7c. The polar lipids included
phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine,
phosphatidylcholine, phospholipid of unknown
structure containing glucosamine, and unidentified
aminolipid. The DNA G + C content was between 64.2 and
64.5 mol%. DNA-DNA hybridization showed 96.7% relatedness
between LM2T and LM4, 24.9% relatedness between
LM2T and R. tolerans EL-172T, and 36.3% relatedness between
LM4 and R. tolerans EL-172T. Based on phylogenetic
analysis, DNA-DNA hybridization, a range of physiological
and biochemical characteristics, LM2T and LM4 belong to
the same species and were clearly distinguished from the type
strains of the genus Roseovarius. It was evident that LM2T
and LM4 could be classified as a novel species of the genus
Roseovarius, for which the name Roseovarius tibetensis sp. nov.
is proposed. The type strain is LM2T (= CGMCC 1.16230T =
KCTC 62028T).
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Citations
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International Journal of Systematic and Evolutionary Microbiology
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- Halomonas tibetensis sp. nov., isolated from saline lakes on Tibetan Plateau
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Hui-bin Lu , Peng Xing , Lei Zhai , Dorji Phurbu , Qian Tang , Qing-long Wu
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J. Microbiol. 2018;56(7):493-499. Published online June 14, 2018
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DOI: https://doi.org/10.1007/s12275-018-8076-5
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996
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Strains pyc13T and ZGT13 were isolated from Lake Pengyan
and Lake Zigetang on Tibetan Plateau, respectively. Both
strains were Gram-negative, catalase- and oxidase-positive,
aerobic, rod-shaped, nonmotile, and nonflagellated bacteria.
Phylogenetic analysis based on 16S rRNA gene sequences
showed that strains pyc13T and ZGT13 belong to the genus
Halomonas, with Halomonas alkalicola 56-L4-10aEnT as
their closest neighbor, showing 97.4% 16S rRNA gene sequence
similarity. The predominant respiratory quinone of
both strains was Q-9, with Q-8 as a minor component. The
major fatty acids of both strains were C18:1 ω6c/C18:1 ω7c,
C16:1 ω6c/C16:1 ω7c, C16:0, and C12:0 3OH. The polar lipids of
both strains consisted of phosphatidylethanolamine, phosphatidylglycerol,
diphosphatidylglycerol, glycolipid, phospholipids
of unknown structure containing glucosamine,
and unidentified phospholipids. The DNA G + C content of
pyc13T and ZGT13 were 62.6 and 63.4 mol%, respectively.
The DNA-DNA hybridization values of strain pyc13T were
34, 41, 61, 35, and 35% with the reference strains H. alkalicola
56-L4-10aEnT, H. sediminicola CPS11T, H. mongoliensis
Z-7009T, H. ventosae Al12T, and H. fontilapidosi 5CRT, respectively.
Phenotypic, biochemical, genotypic, and DNA-DNA
hybridization data showed that strains pyc13T and ZGT13
represent a new species within the genus Halomonas, for
which the name H. tibetensis sp. nov. is proposed. The type
strain is pyc13T (= CGMCC 1.15949T = KCTC 52660T).
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Citations
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International Journal of Systematic and Evolutionary Microbiology
.2020; 70(4): 2859. CrossRef - List of new names and new combinations previously effectively, but not validly, published
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International Journal of Systematic and Evolutionary Microbiology.2018; 68(11): 3379. CrossRef
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