- Development of a Latex Agglutination Test for Norovirus Detection
-
Heetae Lee , YoungBin Park , Misoon Kim , Youngmee Jee , Doo-sung Cheon , Hae Sook Jeong , GwangPyo Ko
-
J. Microbiol. 2010;48(4):419-425. Published online August 20, 2010
-
DOI: https://doi.org/10.1007/s12275-010-0071-4
-
-
690
View
-
0
Download
-
8
Crossref
-
 Abstract
 PDF
-
Norovirus (NoV) is the leading cause of acute gastroenteritis worldwide. Currently, reverse transcription polymerase chain reaction (RT-PCR) is used commonly to detect NoVs in both clinical and environmental samples. However, RT-PCR requires expensive equipment and cannot be performed on site. In this study, a latex agglutination test (LAT) using antibody-labeled latex beads for detecting NoVs was developed. Two kinds of polyclonal antibodies, one generated from synthetic peptides and the other from E. coli-expressed NoV capsid proteins, were used to develop the LAT. Each of these polyclonal antibodies was immobilized on the surface of latex beads and tested for the ability to detect NoVs. Under optimized conditions, our LAT detected GII.4 NoV at concentrations as low as 3.3×105 RT-PCR units/ml in stool samples. The detection limit for the LAT was approximately 1.7×103 RT-PCR units. Forty-eight stool samples were tested for NoVs using this LAT. In comparison with an RT-PCR assay, the sensitivity and specificity of the LAT were 35% and 100%, respectively. With further optimization, this LAT used with appropriate antibodies could be applied for convenient detection of NoVs in clinical diagnosis and food monitoring.
-
Citations
Citations to this article as recorded by  - Viral Eco-Genomic Tools: Development and Implementation for Aquatic Biomonitoring
Gomaa Mostafa-Hedeab, Abdou Kamal Allayeh, Hany Abdelfattah Elhady, Abozer Y. Eledrdery, Mobarak Abu Mraheil, Ahmed Mostafa
International Journal of Environmental Research and Public Health.2022; 19(13): 7707. CrossRef - Comparison of two new in-house Latex Agglutination Tests (LATs), based on the DnaK and Com1 synthetic peptides of Coxiella burnetii, with a commercial indirect-ELISA, for sero-screening of coxiellosis in bovines
Jay Prakash Yadav, Satya Veer Singh Malik, Pankaj Dhaka, Manesh Kumar, Bhoomika Sirsant, Diksha Gourkhede, Sukhadeo B. Barbuddhe, Deepak B. Rawool
Journal of Microbiological Methods.2020; 170: 105859. CrossRef - Development of the Com1 synthetic peptide-based Latex Agglutination Test (LAT) and its comparative evaluation with commercial indirect-ELISA for sero-screening of coxiellosis in cattle
Manesh Kumar, Satyaveer Singh Malik, Jess Vergis, Sunitha Ramanjeneya, Radhakrishna Sahu, Richa Pathak, Jay Prakash Yadav, Pankaj Dhaka, Sukhadeo B. Barbuddhe, Deepak B. Rawool
Journal of Microbiological Methods.2019; 162: 83. CrossRef - Guidelines for the Performance Evaluation of In-Vitro Diagnostic Test for the Detection of Norovirus Infection in Korea
Jayoung Kim, Hyun Soo Kim, Sunwha Lee, Seoung Hwan Oh, Kwang-Sook Woo, Seong-Youl Kim, Eui Kee Min
Laboratory Medicine Online.2017; 7(1): 1. CrossRef - Rapid Waterborne Pathogen Detection with Mobile Electronics
Tsung-Feng Wu, Yu-Chen Chen, Wei-Chung Wang, Ashwini S. Kucknoor, Che-Jen Lin, Yu-Hwa Lo, Chun-Wei Yao, Ian Lian
Sensors.2017; 17(6): 1348. CrossRef - Conventional Rapid Latex Agglutination in Estimation of von Willebrand Factor: Method Revisited and Potential Clinical Applications
Marianor Mahat, Wan Zaidah Abdullah, Che Maraina Che Hussin
Journal of Immunology Research.2014; 2014: 1. CrossRef - The validation and utility of a quantitative one-step multiplex RT real-time PCR targeting Rotavirus A and Norovirus
Tran Thi Ngoc Dung, Voong Vinh Phat, Tran Vu Thieu Nga, Phan Vu Tra My, Pham Thanh Duy, James I. Campbell, Cao Thu Thuy, Nguyen Van Minh Hoang, Pham Van Minh, Hoang Le Phuc, Pham Thi Ngoc Tuyet, Ha Vinh, Duong Thi Hue Kien, Huynh Le Anh Huy, Nguyen Thanh
Journal of Virological Methods.2013; 187(1): 138. CrossRef - Comparison of methods for the detection of coliphages in recreational water at two California, United States beaches
Roberto A. Rodríguez, David C. Love, Jill R. Stewart, Julianne Tajuba, Jacqueline Knee, Jerold W. Dickerson, Laura F. Webster, Mark D. Sobsey
Journal of Virological Methods.2012; 181(1): 73. CrossRef
|
MSK
