Journal of Microbiology

Doo Hyun Park

5 Articles

Symbiotic Relationship between Microbacterium sp. SK0812 and Candida tropicalis SK090404: Seung Won Kang , Bo Young Jeon , Tae Sik Hwang , Doo Hyun Park; J. Microbiol. 2009;47(6):721-727. Published online February 4, 2010; DOI: https://doi.org/10.1007/s12275-009-0146-2

737 View
0 Download
6 Crossref

A bacterium growing inside yeast cytoplasm was observed by light microscope without staining. The bacterium was separately stained from yeast cell by a fluorescent dye, 4′,6-diamidino-2-phenylindole (DAPI). The bacterium actively moved inside yeast cytoplasm and propagated in company with the yeast growth. The bacterium was separated from the yeast cytoplasm by selective disruption of yeast cells and the yeast without the intracellular bacterium (YWOB) was obtained by selective inactivation of bacterial cells. The yeast and the intracellular bacterium were identified as Candida tropicalis and Microbacterium sp., respectively. The length of Microbacterium sp. and C. tropicalis measured with SEM image was smaller than 0.5 μm and was larger than 5 μm, respectively. The yeast with the intracellular bacterium (YWIB) grew in a starch-based medium but the YWOB was not C. tropicalis has neither extracellular nor intracellular saccharification enzyme. Glucose was produced from starch by the extracellular crude enzyme (culture fluid) of Microbacterium sp. YWIB produced significantly more ethanol from glucose than YWOB but did not from starch. Conclusively, C. tropicalis is thought to catabolize starch dependent upon Microbacterium sp. growing in its cytoplasm and furnish stable habitat for the Microbacterium sp.

Citations

Citations to this article as recorded by

Endosymbiotic interactions in the fungal kingdom: a framework for progressive endosymbiosis
Juan Jose Hernandez-Gonzalez, Elva Teresa Arechiga-Carvajal, Olga Miriam Rutiaga-Quiñones, Katia Jamileth Gonzalez-Lozano
Frontiers in Microbiology.2026;[Epub] CrossRef
First Evidence of Metabolically Active Intracellular Bacteria in Saccharomyces cerevisiae
Annabella Tramice, Gianni Liti, Annalaura Iodice, Gennaro Roberto Abbamondi, Federica Carlea, Ernesto Petruzziello, Adele Cutignano, Debora Paris, Carmine Iodice, Matteo De Chiara, Maria Aponte, Francesca De Filippis, Chiara Vischioni, Andrea Motta, Giuse
Journal of Agricultural and Food Chemistry.2025; 73(41): 26220. CrossRef
Tripartite interactions comprising yeast-endobacteria systems in the gut of vector mosquitoes
Alessia Cappelli, Claudia Damiani, Aida Capone, Jovana Bozic, Priscilla Mensah, Emanuela Clementi, Roberta Spaccapelo, Guido Favia, Irene Ricci
Frontiers in Microbiology.2023;[Epub] CrossRef
Uncovering the hidden bacterial ghost communities of yeast and experimental evidences demonstrates yeast as thriving hub for bacteria
B. Indu, Tallapragada Keertana, Sahu Ipsita, Uppada Jagadeeshwari, Chintalapati Sasikala, Chintalapati Venkata Ramana
Scientific Reports.2021;[Epub] CrossRef
Wolbachia pipientis grows in Saccharomyces cerevisiae evoking early death of the host and deregulation of mitochondrial metabolism
Cristina Uribe‐Alvarez, Natalia Chiquete‐Félix, Lilia Morales‐García, Arlette Bohórquez‐Hernández, Norma Laura Delgado‐Buenrostro, Luis Vaca, Antonio Peña, Salvador Uribe‐Carvajal
MicrobiologyOpen.2019;[Epub] CrossRef
Managing plant-environment-symbiont interactions to promote plant performance under low temperature stress
Omid Askari-Khorasgani, Harlene Hatterman-Valenti, Francisco Borja Flores, Mohammad Pessarakli
Journal of Plant Nutrition.2019; 42(16): 2010. CrossRef

Characterization of Exopolysaccharide (EPS) Produced by Weissella hellenica SKkimchi3 Isolated from Kimchi: Min Ju Kim , Ha Na Seo , Tae Sik Hwang , Sung Hun Lee , Doo Hyun Park; J. Microbiol. 2008;46(5):535-541. Published online October 31, 2008; DOI: https://doi.org/10.1007/s12275-008-0134-y

975 View
6 Download
47 Crossref

Abstract PDF

Weissella hellenica SKkimchi3 produces the higher exopolysaccharide (EPS) on sucrose than lactose, glucose, and fructose at pH 5 and 20°C. Sucrose was exclusively used to cultivate SKkimchi3 in all experiments base on the EPS production tests. The molecular mass of EPS, as determined by gel permeation chromatography, was 203,000. 1H and 13C NMR analysis indicated that the identity of EPS may be a glucan. When EPS, starch, and cellulose was treated with α-amylase, glucoamylase, glucosidase, and cellulase, glucose was produced from starch and cellulose but was not produced from EPS. Based on HPLC analysis, elemental analysis, 1H and 13C NMR analysis, and enzymatic hydrolysis tests, EPS was estimated to be a glucan. EPS suspension was not precipitated even by centrifugation at 10,000×g for 60 min, and EPS made the fermented milk and bacterial culture viscous.

Citations

Citations to this article as recorded by

Extracellular biosynthesis of silver chloride nanoparticles by Streptococcus Spp. isolated from Algerian camel smen: antioxidant, antibacterial and antibiofilm potentials
Siham Khenfer, M’hamed Bouricha, Said Mosbah, Roukia Hammoudi, Oussama Bacha, Ouidad Baka, Salah Eddine Laouini, Hakim Belkhalfa
Biomass Conversion and Biorefinery.2026;[Epub] CrossRef
A mannose-rich exopolysaccharide from Weissella cibaria SK01: Purification, characterization, antibiofilm, anticancer, and physicochemical properties
Sahar Javadi, Sedighe Mehrabian, Fatemeh Ashrafi, Hossein Shahbani Zahiri, Kambiz Akbari Noghabi
International Journal of Biological Macromolecules.2026; 382: 154481. CrossRef
A novel exopolysaccharide-producing bacterium from traditional rice wine koji in China: Whole genome sequencing, exopolysaccharide isolation and bio-functional properties assessment
Xiayu Liu, Ying Shi, Donghong Liu, Qihe Chen
LWT.2025; 217: 117375. CrossRef
Exploration of Exopolysaccharide from Leuconostoc mesenteroides HDE-8: Unveiling Structure, Bioactivity, and Food Industry Applications
Yi Yang, Guangbin Ye, Xintong Qi, Bosen Zhou, Liansheng Yu, Gang Song, Renpeng Du
Polymers.2024; 16(7): 954. CrossRef
Technological and genotypic characteristics of lactic acid bacteria isolated from Algerian artisanal dairy products
Malika Bouchibane, Abderrahim Cheriguene, Fadela Chougrani, Mohamed Bououdina, Amel Kaced, Abdelkader Elamine Dahou, Bouasria Benbouziane, Djamel Ait Saada
International Dairy Journal.2023; 146: 105747. CrossRef
Purification, characterization and probiotic proliferation effect of exopolysaccharides produced by Lactiplantibacillus plantarum HDC-01 isolated from sauerkraut
Liansheng Yu, Guangbin Ye, Xintong Qi, Yi Yang, Bosen Zhou, Yunye Zhang, Renpeng Du, Jingping Ge, Wenxiang Ping
Frontiers in Microbiology.2023;[Epub] CrossRef
Variability of Bacterial Homopolysaccharide Production and Properties during Food Processing
Marion Nabot, Marie Guérin, Dharini Sivakumar, Fabienne Remize, Cyrielle Garcia
Biology.2022; 11(2): 171. CrossRef
Cellular antioxidant properties of nontoxic exopolysaccharide extracted from Lactobacillales (Weissella cibaria) isolated from Korean kimchi
Soyoung Park, Kandasamy Saravanakumar, Anbazhagan Sathiyaseelan, SeonJu Park, Xiaowen Hu, Myeong-Hyeon Wang
LWT.2022; 154: 112727. CrossRef
Isolation and characterization of exopolysaccharide derived from Lacticaseibacillus paracasei AS20(1) with probiotic potential and evaluation of its antibacterial activity
E. Amini, F. Salimi, S. Imanparast, F.N. Mansour
Letters in Applied Microbiology.2022; 75(4): 967. CrossRef
Characterization of exopolysaccharide produced by Levilactobacillus brevis HDE-9 and evaluation of its potential use in dairy products
Renpeng Du, Liansheng Yu, Ningxin Yu, Wenxiang Ping, Gang Song, Jingping Ge
International Journal of Biological Macromolecules.2022; 217: 303. CrossRef
Probiotic potential of Weissella paramesenteroides MYPS5.1 isolated from customary dairy products and its therapeutic application
Monika Yadav, Sunita, Pratyoosh Shukla
3 Biotech.2022;[Epub] CrossRef
Protein Expression Level Changes in Weissella koreensis during Garlic Media Fermentation
Youn-Jin Park, Myoung-Jun Jang
Biology.2021; 10(6): 478. CrossRef
In-Depth Investigation of the Safety of Wooden Shelves Used for Traditional Cheese Ripening
Luca Settanni, Gabriele Busetta, Valeria Puccio, Giuseppe Licitra, Elena Franciosi, Luigi Botta, Rosalia Di Gerlando, Massimo Todaro, Raimondo Gaglio, Johanna Björkroth
Applied and Environmental Microbiology.2021;[Epub] CrossRef
Optimization and characterization of exopolysaccharides with a highly branched structure extracted from Leuconostoc citreum B-2
Yu Wang, Renpeng Du, Xiaoxiao Qiao, Bo Zhao, Zhijiang Zhou, Ye Han
International Journal of Biological Macromolecules.2020; 142: 73. CrossRef
Some probiotic potential of Weissella confusa MD1 and Weissella cibaria MD2 isolated from fermented batter
Avinash Kant Lakra, Latha Domdi, Gagan Hanjon, Younus Mohd Tilwani, Venkatesan Arul
LWT.2020; 125: 109261. CrossRef
Overview of exopolysaccharides produced by Weissella genus – A review
Digambar Kavitake, Palanisamy Bruntha Devi, Prathapkumar Halady Shetty
International Journal of Biological Macromolecules.2020; 164: 2964. CrossRef
Prebiotic, Probiotic, Antimicrobial, and Functional Food Applications of Bacillus amyloliquefaciens
Kalekristos WoldemariamYohannes, Zhen Wan, Qinglin Yu, Hongyan Li, Xuetuan Wei, Yingli Liu, Jing Wang, Baoguo Sun
Journal of Agricultural and Food Chemistry.2020; 68(50): 14709. CrossRef
Enhanced granulation process, a more effective way of aerobic granular sludge cultivation in pilot-scale application comparing to normal granulation process: From the perspective of microbial insights
Ding-Chang Li, Jing-Feng Gao, Shu-Jun Zhang, Yong-Qing Gao, Li-Xin Sun
Science of The Total Environment.2020; 707: 136106. CrossRef
Influence of different culture conditions on exopolysaccharide production by indigenous lactic acid bacteria isolated from pickles
Fazilet Mıdık, Mehmet Tokatlı, Simel Bağder Elmacı, Filiz Özçelik
Archives of Microbiology.2020; 202(4): 875. CrossRef
Optimization production of exopolysaccharide from Leuconostoc lactis L2 and its partial characterization
Jing Jiang, Shangxu Guo, Wenxiang Ping, Dan Zhao, Jingping Ge
International Journal of Biological Macromolecules.2020; 159: 630. CrossRef
The effects of exopolysaccharides and exopolysaccharide-producing Lactobacillus on the intestinal microbiome of zebrafish (Danio rerio)
Chenchen Ma, Hongyang Guo, Haibo Chang, Shi Huang, Shuaiming Jiang, Dongxue Huo, Jiachao Zhang, Xiaopeng Zhu
BMC Microbiology.2020;[Epub] CrossRef
Usage of in situ exopolysaccharide‐forming lactic acid bacteria in food production: Meat products—A new field of application?
Myriam Loeffler, Jonas Hilbig, Lina Velasco, Jochen Weiss
Comprehensive Reviews in Food Science and Food Safety.2020; 19(6): 2932. CrossRef
Exopolysaccharide produced by Weissella confusa: Chemical characterisation, rheology and bioactivity
Insaf Samira Benhouna, Arnaud Heumann, Aurélie Rieu, Jean Guzzo, Mabrouk Kihal, Guessas Bettache, Dominique Champion, Christian Coelho, Stephanie Weidmann
International Dairy Journal.2019; 90: 88. CrossRef
Purification and characterization of dextran produced by Leuconostoc pseudomesenteroides PC as a potential exopolysaccharide suitable for food applications
Binbin Wang, Qiaozhi Song, Fangkun Zhao, Huazhi Xiao, Zhijiang Zhou, Ye Han
Process Biochemistry.2019; 87: 187. CrossRef
Exopolysaccharides produced by lactic acid bacteria and Bifidobacteria: Structures, physiochemical functions and applications in the food industry
Yuanmei Xu, Yanlong Cui, Fangfang Yue, Lihua Liu, Yuanyuan Shan, Bianfang Liu, Yuan Zhou, Xin Lü
Food Hydrocolloids.2019; 94: 475. CrossRef
Isolation and structural characterization of levan produced by probiotic Bacillus tequilensis-GM from Tunisian fermented goat milk
Yousra Abid, Samia Azabou, Angela Casillo, Houda Gharsallah, Nawel Jemil, Rosa Lanzetta, Hamadi Attia, Maria Michela Corsaro
International Journal of Biological Macromolecules.2019; 133: 786. CrossRef
Isolation and characterization of dextran produced by Lactobacillus sakei L3 from Hubei sausage
Binbin Wang, Qiaozhi Song, Fangkun Zhao, Lixia Zhang, Ye Han, Zhijiang Zhou
Carbohydrate Polymers.2019; 223: 115111. CrossRef
Immunomodulatory effects of exopolysaccharides produced by Bacillus licheniformis and Leuconostoc mesenteroides isolated from Korean kimchi
Sun-Young Kook, Yaelim Lee, Eui-Cheon Jeong, Seokjin Kim
Journal of Functional Foods.2019; 54: 211. CrossRef
Isolation and characterization of high exopolysaccharide-producing Weissella confusa VP30 from young children’s feces
Hui Jin, Yunju Jeong, Sang-Ho Yoo, Tony V. Johnston, Seockmo Ku, Geun Eog Ji
Microbial Cell Factories.2019;[Epub] CrossRef
A functional and genetic overview of exopolysaccharides produced by Lactobacillus plantarum
Yunyun Jiang, Zhennai Yang
Journal of Functional Foods.2018; 47: 229. CrossRef
Functional Probiotic Assessment andIn VivoCholesterol-Lowering Efficacy ofWeissellasp. Associated with Arid Lands Living-Hosts
Imene Fhoula, Amel Rehaiem, Afef Najjari, Donatella Usai, Abdellatif Boudabous, Leonardo Antonio Sechi, Ouzari Hadda-Imene
BioMed Research International.2018; 2018: 1. CrossRef
Fruits and vegetables, as a source of nutritional compounds and phytochemicals: Changes in bioactive compounds during lactic fermentation
Axelle Septembre-Malaterre, Fabienne Remize, Patrick Poucheret
Food Research International.2018; 104: 86. CrossRef
Why Are Weissella spp. Not Used as Commercial Starter Cultures for Food Fermentation?
Amandine Fessard, Fabienne Remize
Fermentation.2017; 3(3): 38. CrossRef
Optimization of dextran production by Weissella cibaria NITCSK4 using Response Surface Methodology-Genetic Algorithm based technology
J. Kanimozhi, I. Ganesh Moorthy, R. Sivashankar, V. Sivasubramanian
Carbohydrate Polymers.2017; 174: 103. CrossRef
Heterofermentative lactic acid bacteria dominate in Korean commercial kimchi
Hee-Young Kim, Yeon-Ju Bong, Ji-Kang Jeong, Sungbum Lee, Byung-Yong Kim, Kun-Young Park
Food Science and Biotechnology.2016; 25(2): 541. CrossRef
Isolation and characterization of dextran produced by Leuconostoc citreum NM105 from manchurian sauerkraut
Yanping Yang, Qian Peng, Yanyun Guo, Ye Han, Huazhi Xiao, Zhijiang Zhou
Carbohydrate Polymers.2015; 133: 365. CrossRef
The controversial nature of the Weissella genus: technological and functional aspects versus whole genome analysis-based pathogenic potential for their application in food and health
Hikmate Abriouel, Leyre Lavilla Lerma, María del Carmen Casado Muñoz, Beatriz Pérez Montoro, Jan Kabisch, Rohtraud Pichner, Gyu-Sung Cho, Horst Neve, Vincenzina Fusco, Charles M. A. P. Franz, Antonio Gálvez, Nabil Benomar
Frontiers in Microbiology.2015;[Epub] CrossRef
Formulation of iron oxide nanoparticles using exopolysaccharide: evaluation of their antibacterial and anticancer activities
Venkatasamy Vignesh, Ganesan Sathiyanarayanan, Gnanasekar Sathishkumar, Karuppaiah Parthiban, Kamaraj Sathish-Kumar, Ramasamy Thirumurugan
RSC Advances.2015; 5(35): 27794. CrossRef
Production of EPS under Cr(VI) challenge in two indigenous bacteria isolated from a tannery effluent
Rida Batool, Kim Yrjälä, Kamran Shaukat, Nazia Jamil, Shahida Hasnain
Journal of Basic Microbiology.2015; 55(9): 1064. CrossRef
Pyrosequencing analysis of microbiota reveals that lactic acid bacteria are dominant in Korean flat fish fermented food,gajami-sikhae
Hyo Jin Kim, Min-Jeong Kim, Timothy Lee Turner, Bong-Soo Kim, Kyung-Mo Song, Sung Hun Yi, Myung-Ki Lee
Bioscience, Biotechnology, and Biochemistry.2014; 78(9): 1611. CrossRef
Impact of High Hydrostatic Pressure Treatment on Microbial Communities in Chinese Water-Boiled Salted Duck
Yulin Feng, Keping Ye, Huhu Wang, Yi Sun, Xinglian Xu, Dengyong Liu, Guanghong Zhou
Journal of Food Protection.2014; 77(7): 1142. CrossRef
Structural and biocompatibility properties of dextran fromWeissella cibariaJAG8 as food additive
Jagan Mohan Rao Tingirikari, Damini Kothari, Rishikesh Shukla, Arun Goyal
International Journal of Food Sciences and Nutrition.2014; 65(6): 686. CrossRef
Evidence for xylooligosaccharide utilization inWeissellastrains isolated from Indian fermented foods and vegetables
Ami Patel, Peter Falck, Nihir Shah, Peter Immerzeel, Patrick Adlercreutz, Henrik Stålbrand, Jashbhai B. Prajapati, Olle Holst, Eva Nordberg Karlsson
FEMS Microbiology Letters.2013; 346(1): 20. CrossRef
Dextran-like exopolysaccharide-producing Leuconostoc and Weissella from kimchi and its ingredients
Ji-Hee Park, Hyo-Ju Ahn, Sung-gun Kim, Chang-Ho Chung
Food Science and Biotechnology.2013; 22(4): 1047. CrossRef
Characterization of glucansucrase and dextran from Weissella sp. TN610 with potential as safe food additives
Wacim Bejar, Valérie Gabriel, Myriam Amari, Sandrine Morel, Monia Mezghani, Emmanuelle Maguin, Catherine Fontagné-Faucher, Samir Bejar, Hichem Chouayekh
International Journal of Biological Macromolecules.2013; 52: 125. CrossRef
Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3
Rifat Z. Ahmed, Khaizran Siddiqui, Muhammad Arman, Nuzhat Ahmed
Carbohydrate Polymers.2012; 90(1): 441. CrossRef
Comparative evaluation of antioxidant, nitrite scavenging, and antitumor effects of Antrodia camphorata extract
Wol-Suk Cha, Ji-Lu Ding, DuBok Choi
Biotechnology and Bioprocess Engineering.2009; 14(2): 232. CrossRef

Electrochemical Reduction of Xylose to Xylitol by Whole Cells or Crude Enzyme of Candida peltata: Sun Mi Park , Byung In Sang , Dae Won Park , Doo Hyun Park; J. Microbiol. 2005;43(5):451-455.; DOI: https://doi.org/2276 [pii]

525 View
1 Download

Abstract PDF: In this study, whole cells and a crude enzyme of Candida peltata were applied to an electrochemical bioreactor, in order to induce an increment of the reduction of xylose to xylitol. Neutral red was utilized as an electron mediator in the whole cell reactor, and a graphite-Mn(IV) electrode was used as a catalyst in the enzyme reactor in order to induce the electrochemical reduction of NAD+ to NADH. The efficiency with which xylose was converted to xylitol in the electrochemical bioreactor was five times higher than that in the conventional bioreactor, when whole cells were employed as a biocatalyst. Meanwhile, the xylose to xylitol reduction efficiency in the enzyme reactor using the graphite-Mn (IV) electrode and NAD+ was twice as high as that observed in the conventional bioreactor which utilized NADH as a reducing power. In order to use the graphite-Mn(IV) electrode as a catalyst for the reduction of NAD+ to NADH, a bioelectrocatalyst was engineered, namely, oxidoreductase (e.g. xylose reductase). NAD+ can function in this biotransformation procedure without any electron mediator or a second oxidoreductase for NAD+/NADH recycling

Growth and Physiological Properties of Wild Type and Mutants of Halomonas subglaciescola DH-1 in Saline Environment: Hye Jeong Ryu , Yoo Jung Jeong , Doo Hyun Park; J. Microbiol. 2004;42(3):174-180.; DOI: https://doi.org/2093 [pii]

493 View
1 Download

Abstract PDF: A halophilic bacterium was isolated from fermented seafood. The 16S rDNA sequence identity between the isolate and Halomonas subglaciescola AJ306801 was above 95%. The isolate that did not grow in the condition without NaCl or in the condition with other sodium (Na^+) or chloride ions (Cl^-) instead of NaCl was named H. subglaciescola DH-1. Two mutants capable of growing without NaCl were obtained by random mutagenesis, of which their total soluble protein profiles were compared with those of the wild type by two-dimensional electrophoresis. The external compatible solutes (betaine and choline) and cell extract of the wild type did not function as osmoprotectants, and these parameters within the mutants did not enhance their growth in the saline environment. In the proton translocation test, rapid acidification of the reactant was not detected for the wild type, but it was detected for the mutant in the condition without NaCl. From these results, we derived the hypothesis that NaCl may be absolutely required for the energy metabolism of H. subglaciescola DH-1 but not for its osmoregulation, and the mutants may have another modified proton translocation system that is independent of NaCl, except for those mutants with an NaCl-dependent system.

Growth Properties of the Iron-reducing Bacteria, Shewanella putrefaciens IR-1 and MR-1 Coupling to Reduction of Fe(III) to Fe(II): Doo Hyun Park , Byung Hong Kim; J. Microbiol. 2001;39(4):273-278.

788 View
2 Download

Abstract PDF: Shewanella putrefaciens IR-1 and MR-1 were cultivated by using various combinations of electron donor-acceptor, lactate-Fe(III), lactate-nitrate, pyruvate-Fe(III), pyruvate-nitrate, H_2 -acetate-Fe(III) and H_2 -acetate-nitrate. Both strains grew fermentatively on pyruvate but not on lactate without an electron acceptor. In culture with Fe(III), both strains grew on pyruvate and lactate but not on H_2 -acetate-CO_2 . In cultivation with nitrate, both strains grew on pyruvate, lactate and on H_2 -acetate-CO_2 . The growth yields of IR-1 on pyruvate, pyruvate-Fe(III) and lactate-Fe(III) were about 3.4, 3.5, and 3.6 (g cell/M substrate), respectively, but the yields on lactate-nitrate, pyruvate-nitrate and H_2 -acetate-CO_2 ?trate were about 6.8, 5.9, and 9.4 (g cell/M substrate), respectively. From the growth properties of both strains on media with Fe(III) as an electron acceptor, the bacterial growth was confirmed not to be increased by addition of Fe(III) as an electron acceptor to the growth medium, which indicates a possibility that the dissimilatory reduction of Fe(III) to Fe(II) may not be coupled to free energy production.

Author index