- The type II histidine triad protein HtpsC facilitates invasion of epithelial cells by highly virulent Streptococcus suis serotype 2
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Yunjun Lu , Shu Li , Xiaodong Shen , Yan Zhao , Dongming Zhou , Dan Hu , Xushen Cai , Lixia Lu , Xiaohui Xiong , Ming Li , Min Cao
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J. Microbiol. 2021;59(10):949-957. Published online September 7, 2021
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DOI: https://doi.org/10.1007/s12275-021-1129-1
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 Abstract
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Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic
pathogen that presents a significant threat both to pigs
and to workers in the pork industry. The initial steps of S. suis
2 pathogenesis are unclear. In this study, we found that the
type II histidine triad protein HtpsC from the highly virulent
Chinese isolate 05ZYH33 is structurally similar to internalin
A (InlA) from Listeria monocytogenes, which plays an important
role in mediating listerial invasion of epithelial cells. To
determine if HtpsC and InlA function similarly, an isogenic
htpsC mutant (ΔhtpsC) was generated in S. suis by homologous
recombination. The htpsC deletion strain exhibited a
diminished ability to adhere to and invade epithelial cells from
different sources. Double immunofluorescence microscopy
also revealed reduced survival of the ΔhtpsC mutant after cocultivation
with epithelium. Adhesion to epithelium and invasion
by the wild type strain was inhibited by a monoclonal
antibody against E-cadherin. In contrast, the htpsC-deficient
mutant was unaffected by the same treatment, suggesting that
E-cadherin is the host-cell receptor that interacts with HtpsC
and facilitates bacterial internalization. Based on these results,
we propose that HtpsC is involved in the process by which
S. suis 2 penetrates host epithelial cells, and that this protein
is an important virulence factor associated with cell adhesion
and invasion.
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Citations
Citations to this article as recorded by  - Rapid and specific detection of Streptococcus suis serotype 2 using a RPA–PfAgo system coupled with fluorescence and lateral flow dipstick
Kexin Wang, Xujie Zhao, Jingjing Li, Mingzhu Zhou, Bingze Jiao, Yilin Bai, Zhanwei Teng, Meinan Chang, Yueyu Bai, Jianhe Hu, Ke Ding, Xiaojing Xia
Frontiers in Veterinary Science.2026;[Epub] CrossRef - HtpsB, a type II histidine triad protein of Streptococcus suis, contributes to bacterial virulence and blood-brain barrier disruption
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Veterinary Microbiology.2026; 320: 111143. CrossRef -
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Virulence.2026;[Epub] CrossRef - Innate immune response to Streptococcus suis infection: advances and future challenges
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Cristina Uruén, Ana Fernandez, José Luis Arnal, Mateo del Pozo, Maria Casas Amoribieta, Ignacio de Blas, Paula Jurado, Jorge Hugo Calvo, Marcelo Gottschalk, Luis Daniel González-Vázquez, Miguel Arenas, Clara M. Marín, Jesús Arenas
Veterinary Research.2024;[Epub] CrossRef - A rapid colloidal gold immunochromatographic assay based on polyclonal antibodies against HtpsC protein for the detection of Streptococcus suis
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