Journal of Microbiology

DongGeRaMi Moon

3 Articles

Isolation of Synthetic Lethal Mutations in the rsm1-null Mutant of Fission Yeast: DongGeRaMi Moon , Yun-Sun Park , Cha-Yeon Kim , Jin Ho Yoon; J. Microbiol. 2010;48(5):701-705. Published online November 3, 2010; DOI: https://doi.org/10.1007/s12275-010-0353-x

765 View
0 Download
2 Crossref

To identify mutations in genes that are genetically linked to rsm1, we performed a synthetic lethal genetic screen in the fission yeast, Schizosaccharomyces pombe. Four mutations that showed synthetic lethality in combination with the rsm1null allele were isolated from approximately 320,000 colonies and defined in three complementation groups. One mutant (SLrsm1) exhibited a significant accumulation of poly(A)+ RNA in the nucleus under synthetic lethal conditions, while the rest had no mRNA export defects. In addition, some genes (spmex67, rae1, or mlo3) required for mRNA export complemented the growth defects of the identified mutants. These results suggest that the isolated mutants contain mutations in genes that are involved in mRNA export and/or pre-mRNA retention.

Citations

Citations to this article as recorded by

Structure of the pre-mRNA leakage 39-kDa protein reveals a single domain of integrated zf-C3HC and Rsm1 modules
Hideharu Hashimoto, Daniel H. Ramirez, Ophélie Lautier, Natalie Pawlak, Günter Blobel, Benoît Palancade, Erik W. Debler
Scientific Reports.2022;[Epub] CrossRef
Effects of spThoc7 Deletion on Growth and mRNA Export in Fission Yeast
Eun-Jin Koh, Jin Ho Yoon
The Korean Journal of Microbiology.2014; 50(3): 249. CrossRef

Nup211, the Fission Yeast Homolog of Mlp1/Tpr, Is Involved in mRNA Export: Jin-Ah Bae , DongGeRaMi Moon , Jin Ho Yoon; J. Microbiol. 2009;47(3):337-343. Published online June 26, 2009; DOI: https://doi.org/10.1007/s12275-009-0125-7

910 View
0 Download
8 Crossref

Abstract PDF

Synthetic lethal mutants have been previously isolated in fission yeast Schizosaccharomyces pombe, which genetically interact with spmex67, in order to identify the genes involved in mRNA export. The nup211 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex2, under synthetic lethal condition. We showed that Nup211, fission yeast homolog of Mlp1/Mlp2/Tpr, is essential for vegetative growth and Nup211-GFP proteins expressed at endogenous level are localized mainly in nuclear periphery. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of nup211 is repressed or over-expressed. These results suggest that the Nup211 protein plays a pivotal role of mRNA export in fission yeast.

Citations

Citations to this article as recorded by

Fission yeast essential nuclear pore protein Nup211 regulates the expression of genes involved in cytokinesis
Domenick Kamel, Ayisha Sookdeo, Ayana Ikenouchi, Hualin Zhong, Juan Mata
PLOS ONE.2024; 19(12): e0312095. CrossRef
Quantitative analysis of nuclear pore complex organization in Schizosaccharomyces pombe
Joseph M Varberg, Jay R Unruh, Andrew J Bestul, Azqa A Khan, Sue L Jaspersen
Life Science Alliance.2022; 5(7): e202201423. CrossRef
Asymmetrical localization of Nup107-160 subcomplex components within the nuclear pore complex in fission yeast
Haruhiko Asakawa, Tomoko Kojidani, Hui-Ju Yang, Chizuru Ohtsuki, Hiroko Osakada, Atsushi Matsuda, Masaaki Iwamoto, Yuji Chikashige, Koji Nagao, Chikashi Obuse, Yasushi Hiraoka, Tokuko Haraguchi, Gregory P. Copenhaver
PLOS Genetics.2019; 15(6): e1008061. CrossRef
The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components
Silvia Salas-Pino, Paola Gallardo, Ramón R. Barrales, Sigurd Braun, Rafael R. Daga
Journal of Cell Biology.2017; 216(11): 3591. CrossRef
Tts1, the fission yeast homologue of the TMEM33 family, functions in NE remodeling during mitosis
Dan Zhang, Snezhana Oliferenko, Daniel J. Lew
Molecular Biology of the Cell.2014; 25(19): 2970. CrossRef
Characterization of nuclear pore complex components in fission yeastSchizosaccharomyces pombe
Haruhiko Asakawa, Hui-Ju Yang, Takaharu G Yamamoto, Chizuru Ohtsuki, Yuji Chikashige, Kumiko Sakata-Sogawa, Makio Tokunaga, Masaaki Iwamoto, Yasushi Hiraoka, Tokuko Haraguchi
Nucleus.2014; 5(2): 149. CrossRef
Isolation of synthetic lethal mutations in combination with spnab2 of fission yeast
Yun-Sun Park, Jin Ho Yoon
Genes & Genomics.2012; 34(3): 275. CrossRef
Double duty for nuclear proteins – the price of more open forms of mitosis
Colin P. De Souza, Stephen A. Osmani
Trends in Genetics.2009; 25(12): 545. CrossRef

The Fission Yeast Homologue of Gle1 is Essential for Growth and Involved in mRNA Export: DongGeRaMi Moon , Jin-Ah Bae , Hyun Jin Cho , Jin Ho Yoon; J. Microbiol. 2008;46(4):422-428. Published online August 31, 2008; DOI: https://doi.org/10.1007/s12275-008-0177-0

823 View
0 Download
4 Crossref

Abstract PDF

We have isolated Gle1 homologue (named as spgle1) as a partial multicopy suppressor of the synthetic lethality of rae1-167 elf1-21 in fission yeast Schizosaccharomyces pombe. The spgle1 is also able to complement partially temperature-sensitive phenotype of rae1-167 only at a lower restrictive temperature. The spgle1 gene contains one intron and encodes a 480 amino-acid protein with predicted molecular weight of 56.2 kDa. We showed that spgle1 gene is essential for vegetative growth and functional Gle1-GFP protein is localized mainly in NPC. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of spgle1 is repressed or over-expressed. These results suggest that the spGle1 protein is also involved in mRNA export in fission yeast.

Citations

Citations to this article as recorded by

Unveiling the Plasmodium inositol (pyro)phosphate pathway: Highlighting inositol polyphosphate multikinase as a novel therapeutic target for malaria
Abigail Obuobi, Neils B. Quashie, Nancy Odurowah Duah-Quashie, Jon R. Sayers, Yash Gupta
PLOS One.2025; 20(12): e0338411. CrossRef
Isolation of synthetic lethal mutations in combination with spnab2 of fission yeast
Yun-Sun Park, Jin Ho Yoon
Genes & Genomics.2012; 34(3): 275. CrossRef
Control of mRNA Export and Translation Termination by Inositol Hexakisphosphate Requires Specific Interaction with Gle1
Abel R. Alcázar-Román, Timothy A. Bolger, Susan R. Wente
Journal of Biological Chemistry.2010; 285(22): 16683. CrossRef
Generation of expression vectors for high-throughput functional analysis of target genes in Schizosaccharomyces pombe
Jiwon Ahn, Chung-Hae Choi, Chang-Mo Kang, Chun-Ho Kim, Hee-Moon Park, Kyung-Bin Song, Kwang-Lae Hoe, Misun Won, Kyung-Sook Chung
The Journal of Microbiology.2009; 47(6): 789. CrossRef

Author index