- Structural analysis of N-/O-glycans assembled on proteins in yeasts
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Eun Jung Thak , Jungho Kim , Dong-Jik Lee , Jeong Yoon Kim , Hyun Ah Kang
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J. Microbiol. 2018;56(1):11-23. Published online January 4, 2018
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DOI: https://doi.org/10.1007/s12275-018-7468-x
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 Abstract
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Protein glycosylation, the most universal and diverse posttranslational
modification, can affect protein secretion, stability,
and immunogenicity. The structures of glycans attached
to proteins are quite diverse among different organisms and
even within yeast species. In yeast, protein glycosylation plays
key roles in the quality control of secretory proteins, and particularly
in maintaining cell wall integrity. Moreover, in pathogenic
yeasts, glycans assembled on cell-surface glycoproteins
can mediate their interactions with host cells. Thus, a
comprehensive understanding of protein glycosylation in various
yeast species and defining glycan structure characteristics
can provide useful information for their biotechnological
and clinical implications. Yeast-specific glycans are a target
for glyco-engineering; implementing human-type glycosylation
pathways in yeast can aid the production of recombinant
glycoproteins with therapeutic potential. The virulenceassociated
glycans of pathogenic yeasts could be exploited
as novel targets for antifungal agents. Nowadays, several glycomics
techniques facilitate the generation of species- and
strain-specific glycome profiles and the delineation of modified
glycan structures in mutant and engineered yeast cells.
Here, we present the protocols employed in our laboratory
to investigate the N- and O-glycan chains released from purified
glycoproteins or cell wall mannoproteins in several
yeast species.
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Citations
Citations to this article as recorded by  - Conserved Golgi nucleoside diphosphatases drive protein glycosylation and virulence in Fusarium graminearum
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Phytopathology Research.2026;[Epub] CrossRef - Evolutionary unique N-glycan-dependent protein quality control system plays pivotal roles in cellular fitness and extracellular vesicle transport in Cryptococcus neoformans
Catia Mota, Kiseung Kim, Ye Ji Son, Eun Jung Thak, Su-Bin Lee, Ju-El Kim, Jeong-Kee Yoon, Min-Ho Kang, Heeyoun Hwang, Yong-Sun Bahn, J Andrew Alspaugh, Hyun Ah Kang
eLife.2025;[Epub] CrossRef - Integrative glycomic analysis reveals the crucial role of protein glycosylation in fungal pathogenesis
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PLOS Pathogens.2025; 21(7): e1013325. CrossRef - Evolutionary unique N-glycan-dependent protein quality control system plays pivotal roles in cellular fitness and extracellular vesicle transport in Cryptococcus neoformans
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eLife.2025;[Epub] CrossRef - Protein Expression Platforms and the Challenges of Viral Antigen Production
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Journal of Agricultural and Food Chemistry.2024; 72(11): 6028. CrossRef - Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2017–2018
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Renewable Energy.2022; 201: 486. CrossRef - Pushing and pulling proteins into the yeast secretory pathway enhances recombinant protein secretion
Richard J. Zahrl, Roland Prielhofer, Özge Ata, Kristin Baumann, Diethard Mattanovich, Brigitte Gasser
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Extension of
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mBio.2022;[Epub] CrossRef - Metabolic labeling of glycans with isotopic glucose for quantitative glycomics in yeast
Ji-Yeon Kim, Woo Hong Joo, Dong-Soo Shin, Yong-Ill Lee, Chin Fen Teo, Jae-Min Lim
Analytical Biochemistry.2021; 621: 114152. CrossRef - Yeast-produced RBD-based recombinant protein vaccines elicit broadly neutralizing antibodies and durable protective immunity against SARS-CoV-2 infection
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Cell Discovery.2021;[Epub] CrossRef - What makes Komagataella phaffii non-conventional?
Özge Ata, Burcu Gündüz Ergün, Patrick Fickers, Lina Heistinger, Diethard Mattanovich, Corinna Rebnegger, Brigitte Gasser
FEMS Yeast Research.2021;[Epub] CrossRef - Water Kefir and Derived Pasteurized Beverages Modulate Gut Microbiota, Intestinal Permeability and Cytokine Production In Vitro
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Jae-Hyung Jin, Kyung-Tae Lee, Joohyeon Hong, Dongpil Lee, Eun-Ha Jang, Jin-Young Kim, Yeonseon Lee, Seung-Heon Lee, Yee-Seul So, Kwang-Woo Jung, Dong-Gi Lee, Eunji Jeong, Minjae Lee, Yu-Byeong Jang, Yeseul Choi, Myung Ha Lee, Ji-Seok Kim, Seong-Ryong Yu,
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David J. Harvey
Mass Spectrometry Reviews.2020; 39(5-6): 586. CrossRef - Yeast synthetic biology for designed cell factories producing secretory recombinant proteins
Eun Jung Thak, Su Jin Yoo, Hye Yun Moon, Hyun Ah Kang
FEMS Yeast Research.2020;[Epub] CrossRef -
Core
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-Glycan Structures Are Critical for the Pathogenicity of Cryptococcus neoformans by Modulating Host Cell Death
Eun Jung Thak, Su-Bin Lee, Shengjie Xu-Vanpala, Dong-Jik Lee, Seung-Yeon Chung, Yong-Sun Bahn, Doo-Byoung Oh, Mari L. Shinohara, Hyun Ah Kang, J. Andrew Alspaugh
mBio.2020;[Epub] CrossRef - Hydrophilic interaction chromatography for the analysis of biopharmaceutical drugs and therapeutic peptides: A review based on the separation characteristics of the hydrophilic interaction chromatography phases
Tohru Ikegami
Journal of Separation Science.2019; 42(1): 130. CrossRef - Impact of ∼omics in the detection and validation of potential anti-infective drugs
Nidia Maldonado-Carmona, Melissa Vázquez-Hernández, Osiris Jair Patiño Chávez, Stefany Daniela Rodríguez-Luna, Omar Jiménez Rodríguez, Sergio Sanchez, Corina Diana Ceapă
Current Opinion in Pharmacology.2019; 48: 1. CrossRef
- Functional Characterization of Extracellular Chitinase Encoded by the YlCTS1 Gene in a Dimorphic Yeast Yarrowia lipolytica
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Jeong-Nam Park , Chang Pyo Han , Dong-Jik Lee , Seon Ah Cheon , Hyun Ah Kang
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J. Microbiol. 2014;52(4):284-291. Published online March 29, 2014
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DOI: https://doi.org/10.1007/s12275-014-4070-8
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888
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Abstract
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The hemiascomycetes yeast Yarrowia lipolytica is a dimorphic yeast with alternating yeast and mycelia forms. Bioinformatic analysis revealed the presence of three putative chitinase genes, YlCTS1, YlCTS2, and YlCTS3, in the Y. lipolytica genome. Here, we demonstrated that the protein of YlCTS1 (YlCts1p), which contains an N-terminal secretion signal peptide, a long C-terminal Ser/Thr-rich domain, and
a chitin-binding domain, is a homologue to Saccharomyces cerevisiae chitinase 1 (ScCts1p). Deletion of YlCTS1 remarkably reduced extracellular endochitinase activity in the culture supernatant of Y. lipolytica and enhanced cell aggregation,
suggesting a role of YlCts1p in cell separation as ScCts1p does in S. cerevisiae. However, loss of YlCts1p function did not affect hyphal formation induced by fetal bovine serum addition. The mass of YlCts1p was dramatically decreased
by jack bean α-mannosidase digestion but not by PNGase F treatment, indicating that YlCts1p is modified only by Omannosylation without N-glycosylation. Moreover, the O-glycan profile of YlCts1p was identical to that of total cell wall
mannoproteins, supporting the notion that YlCts1p can be used as a good model for studying O-glycosylation in this dimorphic yeast.
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Citations
Citations to this article as recorded by - Advancing Ultrasensitive, Drift-Correcting Dual Floating Gate Organic Electrochemical Transistors for Yeast Sensing
Jonathan Harris, Michael Brothers, Victoria Coyle, Steve Kim, Erin Ratcliff
Chemistry of Materials.2024; 36(1): 324. CrossRef - The N-Acetylglucosamine Kinase from Yarrowia lipolytica Is a Moonlighting Protein
Carmen-Lisset Flores, Joaquín Ariño, Carlos Gancedo
International Journal of Molecular Sciences.2021; 22(23): 13109. CrossRef - Recovery and valorization of agri-food wastes and by-products using the non-conventional yeast Yarrowia lipolytica
Davide Gottardi, Lorenzo Siroli, Lucia Vannini, Francesca Patrignani, Rosalba Lanciotti
Trends in Food Science & Technology.2021; 115: 74. CrossRef - Functional analysis of recombinant human and Yarrowia lipolytica O-GlcNAc transferases expressed in Saccharomyces cerevisiae
Hye Ji Oh, Hye Yun Moon, Seon Ah Cheon, Yoonsoo Hahn, Hyun Ah Kang
Journal of Microbiology.2016; 54(10): 667. CrossRef
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