- Characteristics of HIV-Tat Protein Transduction Domain
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Jong-Sub Yoon , Yong-Tae Jung , Seong-Karp Hong , Sun-Hwa Kim , Min-Chul Shin , Dong-Gun Lee , Wan-Shik Shin , Woo-Sung Min , Soon-Young Paik
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J. Microbiol. 2004;42(4):328-335.
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DOI: https://doi.org/2103 [pii]
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 Abstract
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The human immunodeficiency virus type 1 (HIV-1) Tat protein transduction domain (PTD), which contains rich arginine and lysine residues, is responsible for the highly efficient transduction of protein through the plasma membrane. In addition, it can be secreted from infected cells and has the ability to enter neighboring cells. When the PTD of Tat is fused to proteins and exogenously added to cells, the fusion protein can cross plasma membranes. Recent reports indicate that the endogenously expressed Tat fusion protein can demonstrate biodistribution of several proteins. However, intercellular transport and protein transduction have not been observed in some studies. Therefore, this study examined the intercellular transport and protein transduction of the Tat protein. The results showed no evidence of intercellular transport (biodistribution) in a cell culture. Instead, the Tat fusion peptides were found to have a significant effect on the transduction and intercellular localization properties. This suggests that the HIV-1 PTD passes through the plasma membrane in one direction.
- DD1.5k, the Gene Preferentially Expressed in Bloodstream Isolates of Vancomycin-Resistant Enterococcus faecium
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Seung-Han Kim , Dong-Gun Lee , Jin-Hong Yoo , Su-Mi Choi , Jung-Hyun Choi , Wan-Shik Shin , Kyungwon Lee , Dongeun Yong , Wee Gyo Lee , Byung-S. Youn , Moon-Won Kang
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J. Microbiol. 2004;42(2):143-146.
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Abstract
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Vancomycin-resistant Enterococcus faecium (VREFM) is becoming a threatening pathogen. We identified a gene called DD1.5K by differential display-PCR, which was preferentially expressed in the bloodstream isolates of VREFM. Due to its amino acid similarity to transfer complex protein, trsE, and tissue-specific expression, this gene may be involved in virulence of VREFM.
- Detection of Lymphotropic Herpesviruses by Multiplex Polymerase Chain Reaction
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Sang-Tae Park , Seung-Han Kim , Dong-Gun Lee , Jung-Hyun Choi , Wan-Shik Shin , Tai - Gyu Ki m , Soon-Young Paik , Chun-Choo Kim
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J. Microbiol. 2001;39(3):226-228.
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Abstract
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Human lymphotropic herpesvirus is known to be a major pathogen associated with various diseases in bone marrow transplantation (BMT) recipients. A multiplex nested-polymerase chain reaction (PCR) method was developed for the simultaneous detection of human lymphotropic herpesviruses, including Ebstein-Barr virus (EBV), cytomegalovirus (CMV), and human herpesvirus 6 variants A and B (HHV6-A, HHV6-B). To demonstrate the usefulness of multiplex PCR for the analysis of clinical samples, peripheral blood mononuclear cells and serum from BMT recipients were analysed. The results showed that a clear detection could be made between EBV, HCMV and HHV-6. This multiplex PCR assay is an efficient and cost-effective approach to the analysis of large numbers of samples to determine the epidemiological importance of EBV, HCMV and HHV-6.
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