Journal of Microbiology

Dong-Beom Lee

3 Articles

Isolation and Characterization of Self-Fertile Suppressors from the Sterile nsdD Deletion Mutant of Aspergillus nidulans: Dong-Beom Lee , Lee Han Kim , Jin-Pyo Kim , Kap-Hoon Han , Dong-Min Han; J. Microbiol. 2011;49(6):1054-1057. Published online December 28, 2011; DOI: https://doi.org/10.1007/s12275-011-1111-4

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To identify downstream and/or interactive factors of the nsdD gene, which encodes a positive regulator of sexual development of Aspergillus nidulans, suppressor mutants displaying a self-fertile phenotype were isolated from a sterile nsdD deletion mutant. At least five different loci (sndA-E) were identified and genetically analyzed. In the nsdD+ background, most of the suppressors showed a marked increment of sexual development, even under the stress conditions that normally inhibited sexual development. The common phenotype of the suppressor mutants suggested the involvement of the snd genes in the negative regulation of sexual development in response to the environmental factors.

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Inactivation of the global regulator LaeA in Monascus ruber results in a species-dependent response in sporulation and secondary metabolism
Qingpei Liu, Li Cai, Yanchun Shao, Youxiang Zhou, Mu Li, Xiaohong Wang, Fusheng Chen
Fungal Biology.2016; 120(3): 297. CrossRef
A putative APSES transcription factor is necessary for normal growth and development of Aspergillus nidulans
Ji-Yeon Lee, Lee-Han Kim, Ha-Eun Kim, Jae-Sin Park, Kap-Hoon Han, Dong-Min Han
Journal of Microbiology.2013; 51(6): 800. CrossRef
Isolation and Characterization of the gtfA Gene Encoding GAL4-Like Transcription Factor in Aspergillus nidulans
Jae-Sin Park, Dong-Min Han
The Korean Journal of Microbiology.2013; 49(1): 8. CrossRef

Regulation of nsdD Expression in Aspergillus nidulans: Kap Hoon Han , Kyu-Yong Han , Min-Su Kim , Dong-Beom Lee , Jong-Hak Kim , Suhn-Kee Chae , Keon-Sang Chae , Dong-Min Han; J. Microbiol. 2003;41(3):259-261.

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Abstract PDF: The nsdD gene has been predicted to encode a GATA type transcription factor with the type IVb zinc finger DNA binding domain functions in activating sexual development of A. nidulans. In several allelic mutants of nsdD producing truncated NsdD polypeptides lacking the C-terminal zinc finger, the transcription level of nsdD gene was greatly increased. Also in an over-expressed mutant, the transcription under its own promoter was reduced. These results suggest that the expression of nsdD is negatively autoregulated. When the nsdD gene was over-expressed, cleistothecia were formed in excess amounts even in the presence of 0.6M KCl that inhibited sexual development of the wild type. Northern blot analysis revealed that the expression of nsdD was repressed by 0.6M KCl. These results strongly suggest that the inhibition of sexual development by salts was carried out via the nsdD involved regulatory network.

Environmental factors affecting development of Aspergillus nidulans: Kap-Hoon Han , Dong-Beom Lee , Jong-Hak Kim , Min-Su Kim , Kyu-Yong Han , Won-Shin Kim , Young-Soon Park , Heui-Baik Kim^ , Dong-Min Han^; J. Microbiol. 2003;41(1):34-40.

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Abstract PDF: Aspergillus nidulans, a homothalic ascomycete, has a complete sexual reproductive cycle as well as an asexual one. Both sexual and asexual development are known to be genetically programmed, but are also strongly affected by environmental factors including nutrients, light, temperature and osmolarity. We have examined these factors to define favored conditions for fruiting body (cleistothecium) formation. In general, fruiting body formation was enhanced where carbon and nitrogen sources were sufficient. Limitation of C-source caused predominant asexual development while inhibiting sexual development. When higher concentrations of glucose were supplied, more cleistothecia were formed. Other carbon sources including lactose, galactose and glycerol made the fungus develop cleistothecia very well, whereas acetate caused asexual sporulation only. Organic nitrogen sources like casein hydrolysate and glycine, and an increase in nitrate or ammonium concentration also enhanced sexual development. In addition to nutrient effects, low levels of aerobic respiration, caused either by platesealing or treatment with various chemicals, favored sexual development. Carbon limitation, light exposure and a high concentration of salts promoted asexual development preferentially, suggesting that stress conditions may drive the cell to develop asexual sporulation while comfortable and wellnourished growth conditions favored sexual development.

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