- Mutants defective in the production of encapsulin show a tan-phaselocked phenotype in Myxococcus xanthus
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Dohee Kim , Juo Choi , Sunjin Lee , Hyesook Hyun , Kyoung Lee , Kyungyun Cho
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J. Microbiol. 2019;57(9):795-802. Published online June 11, 2019
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DOI: https://doi.org/10.1007/s12275-019-8683-9
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Myxococcus xanthus, a myxobacterium, displays phase variation
between yellow phase and tan phase. We found that
deletion of the encA gene encoding encapsulin and the encF
gene encoding a metalloprotease causes formation of tan
colonies that never transform into yellow colonies. The encA
and encF mutants were defective in the production of DKxanthene
and myxovirescin. They did not produce extracellular
polysaccharides; hence, the cells did not aggregate
in liquid and showed reduced swarming on agar plates. The
mutants had defective sporulation, but were rescued extracellularly
by wild type cells. All these traits indicate that
the encA and encF mutants are likely to be tan-phase-locked,
and encapsulin has a close relationship with phase variation
in M. xanthus. The encA and encF genes are localized in the
same gene cluster, encBAEFG (MXAN_3557~MXAN_3553).
Unlike the encA and encF genes, deletion of other genes in
the cluster did not show tan-phase-locked phenotype.
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Citations
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