- Prevalence of Indigenous Antibiotic‑Resistant Salmonella Isolates and Their Application to Explore a Lytic Phage vB_SalS_KFSSM with an Intra‑Broad Specificity
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Jaein Choe , Su-Hyeon Kim , Ji Min Han , Jong-Hoon Kim , Mi-Sun Kwak , Do-Won Jeong , Mi-Kyung Park
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J. Microbiol. 2023;61(12):1063-1073. Published online January 2, 2024
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DOI: https://doi.org/10.1007/s12275-023-00098-6
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 Abstract
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The consumption of fresh produce has led to increase in antibiotic-resistant (AR) Salmonella outbreaks. In this study, indigenous
Salmonella was isolated from a total of two hundred-two samples including fresh produce and agricultural environmental
samples in Korea. After biochemical confirmation using the Indole, Methyl Red, Voges-Proskauer, Citrate tests, presumable
Salmonella isolates were identified by 16S rRNA sequencing. Identified Salmonella isolates were evaluated for antibiotic
susceptibility against twenty-two antibiotics. The specificity and the efficiency of plating (EOP) of vB_SalS_KFSSM were
evaluated against fifty-three bacterial strains. Twenty-five suspected Salmonella were isolated and confirmed by the positive
result
for methyl red and citrate, of which ten were identified as Salmonella spp. through 16S rRNA gene sequencing. Eight
Salmonella isolates (4.0%, n = 8/202) were resistant to at least one antibiotic, among which five were multi-drug resistant. As
a lytic phage against Salmonella spp. CMGS-1, vB_SalS_KFSSM was isolated from cow manure. The phage was observed as
a tailed phage belonging to the class Caudoviricetes. It exhibited an intra-broad specificity against four indigenous AR Salmonella
isolates, two indigenous Salmonella isolates, and five other Salmonella serotypes with great efficiencies (EOP ≥ 0.75).
Thus, this study suggested the potential of vB_SalS_KFSSM to combat indigenous AR Salmonella.
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Citations
Citations to this article as recorded by  - Functional and Genomic Features of a Lytic
Salmonella Phage vB_StyS_KFSST1 for
Development as New Feed Additive
Su-Hyeon Kim, In Young Choi, Gyu-Sung Cho, Charles M.A.P. Franz, Mi-Kyung Park
Food Science of Animal Resources.2025; 45(4): 1204. CrossRef - Draft genome sequences of Salmonella enterica subsp. enterica isolates from fresh produce and agricultural environments in South Korea
Su-Hyeon Kim, Ji Min Han, Gyu-Sung Cho, Erik Brinks, Charles M. A. P. Franz, Mi-Kyung Park
BMC Research Notes.2025;[Epub] CrossRef
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