- Morphological Diversity of Marine Microorganisms on Different Isolation Media
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Shin Hye Park , Kae Kyoung Kwon , Deuk-Soo Lee , Hong Kum Lee
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J. Microbiol. 2002;40(2):161-165.
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 Abstract
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Isolation frequency of microorganisms from marine sources was examined with different media and samples collected from the coastal area of Cheju Island. From sea water samples, about 1% of microorganisms from the total number of bacteria were recovered. Microorganisms were cultured at the much lower frequency of 10^-4 ?0^-6 from other marine sources, such as sediment, sponges and corals. The frequency of duplicated isolation was examined with 140 morphologically different colonies isolated on different media. Fourteen percent of them exhibited the same morphology on two different media. The duplication frequency of the isolates among three different media was 33%.
- Bacterial Diversity of Culturable Isolates from Seawater and a Marine Coral, Plexauridae sp., near Mun-Sum, Cheju-Island
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Jung-Hyun Lee , Hyun-Hee Shin , Deuk-Soo Lee , Kae Kyung Kwon , Sang-Jin Kim , Hong Kum Lee
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J. Microbiol. 1999;37(4):193-199.
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Abstract
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Fifty-eight strains showing different colony morphological characteristics on various media were isolated from marine coral (Plexauridae sp.) and ambient seawater near Mun-Sum, Cheju-Island in 1998. Bacterial diversity was studies by phylogenetic analysis of the partial 16S rRNA gene sequences. All isolates representing the bacterial domain included affiliates of the high G+C (59%) and los G+C (3%) subdivision of Gram positive bacteria, and the alpha (33%) and gamma (5%) subdivision of the Proteobacteria. The 16S rDNA sequence similarity of the isolates was in the 88.3 to 100% range (average, 95.6%) to reported sequence data. In the comparison of the isolates from marine coarl and ambient seawater, more diverse groups belonging to alpha-proteobacteria were preferentially obtained from seawater.
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