Journal of Microbiology

Derlene Attili-Angelis

1 Article

Production of Saccharogenic and Dextrinogenic Amylases by Rhizomucor pusillus A 13.36: Tony M. Silva , Derlene Attili-Angelis , Ana Flavia Azevedo Carvalho , Roberto Da Silva , Mauricio Boscolo , Eleni Gomes; J. Microbiol. 2005;43(6):561-568.; DOI: https://doi.org/2289 [pii]

Abstract PDF: A newly-isolated thermophilic strain of the zygomycete fungus Rhizomucor pusillus 13.36 produced highly active dextrinogenic and saccharogenic enzymes. Cassava pulp was a good alternative substrate for amylase production. Dextrinogenic and saccharogenic amylases exhibited optimum activities at a pH of 4.0-4.5 and 5.0 respectively and at a temperature of 75oC. The enzymes were highly thermostable, with no detectable loss of saccharogenic or dextrinogenic activity after 1 h and 6 h at 60oC, respectively. The saccharogenic activity was inhibited by Ca2+ while the dextrinogenic was indifferent to this ion. Both activities were inhibited by Fe2+ and Cu2+ Hydrolysis of soluble starch by the crude enzyme yielded 66% glucose, 19.5% maltose, 7.7% maltotriose and 6.6% oligosaccharides.

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