- Comparative Analysis of Superantigen Genes in Staphylococcus xylosus and Staphylococcus aureus Isolates Collected from a Single Mammary Quarter of Cows with Mastitis
-
Karol Fijałkowski , Magdalena Struk , Jolanta Karakulska , Aleksandra Paszkowska , Stefania Giedrys-Kalemba , Helena Masiuk , Danuta Czernomysy-Furowicz , Paweł Nawrotek
-
J. Microbiol. 2014;52(5):366-372. Published online April 11, 2014
-
DOI: https://doi.org/10.1007/s12275-014-3436-2
-
-
971
View
-
0
Download
-
16
Crossref
-
 Abstract
 PDF
-
The purpose of this study was to analyze and compare genes encoding superantigens (SAgs) in Staphylococcus xylosus and Staphylococcus aureus isolates collected simultaneously from milk of the same cows with clinical mastitis. Genes encoding staphylococcal enterotoxins and enterotoxin-like proteins (sea-selu), toxic shock syndrome toxin 1 (tst-1) and exfolia-tive toxins (eta and etd) were investigated. It was found that among 30 isolates of S. xylosus, 16 (53.3%) harbored from 1 to 10 SAg genes. In total, in 16 SAg positive S. xylosus, 11 different enterotoxin genes were detected: sec, sed, seg, seh, sei, selm, seln, selo, selp, ser, selu and one etd gene encoding exfoliative toxin D. The most prevalent genes were ser, selu, and selo. Among all the positive isolates of S. xylosus, a total of 14 different SAg gene combinations were detected. One combination was repeated in 3 isolates, whereas the rest were detected only once. However, in the case of S. aureus all the 30 isolates harbored the same combination of SAg genes: seg, sei, selm, seln, selo and on the basis of PFGE analysis all belonged to the same clonal type. Also noteworthy was the observation that SAg genes detected in S. aureus have also been found in S. xylosus. The findings of this study further extend previous observations that SAg genes are present not only in S. aureus but also in coagulase-negative staphy-lococci, including S. xylosus. Therefore, taking into account that the SAg genes are encoded on mobile genetic elements it is possible that these genes can be transferred between different species of coexisting staphylococci.
-
Citations
Citations to this article as recorded by  - Microbial flora of the respiratory tract and skin of artisanal municipal solid waste handlers in Aba, Abia State, Nigeria
I.W. Nwankwo, N.C. Nwachukwu, E.C. Onwuchekwa, O.C. Okamgba, O.C. Ugbogu
Access Microbiology
.2025;[Epub] CrossRef - Whole genome sequence and comparative genomics analysis of multidrug-resistant Staphylococcus xylosus NM36 isolated from a cow with mastitis in Basrah city
Hassan M. Al-Tameemi, Husam Al-Hraishawi, Murtakab Y. Al-Hejjaj, Noor S. Abdulah, Haider R. Alrafas, Yessar A. Dawood
Journal of Genetic Engineering and Biotechnology.2023; 21(1): 163. CrossRef - Staphylococcus xylosus and Staphylococcus aureus as commensals and pathogens on murine skin
Michael Battaglia, Lee Ann Garrett-Sinha
Laboratory Animal Research.2023;[Epub] CrossRef - Identification of the Enterotoxigenic Potential of Staphylococcus spp. from Raw Milk and Raw Milk Cheeses
Patryk Wiśniewski, Joanna Gajewska, Anna Zadernowska, Wioleta Chajęcka-Wierzchowska
Toxins.2023; 16(1): 17. CrossRef - Identification, Superantigen Toxin Gene Profile and Antimicrobial Resistance of Staphylococci Isolated from Polish Primitive Sheep Breeds
Jolanta Karakulska, Marta Woroszyło, Małgorzata Szewczuk, Karol Fijałkowski
Animals.2022; 12(16): 2139. CrossRef - Relationship between L-lactate dehydrogenase and multidrug resistance in Staphylococcus xylosus
Zhongwei Yuan, Jinpeng Wang, Ruixiang Che, Bello-Onaghise God’spower, Yonghui Zhou, Chunliu Dong, Lu Li, Mingri Chen, Nsabimana Eliphaz, Xin Liu, Yanhua Li
Archives of Microbiology.2022;[Epub] CrossRef - Prevalence and distribution of multilocus sequence types of Staphylococcus aureus isolated from bulk tank milk and cows with mastitis in Pennsylvania
Asha Thomas, Shubhada Chothe, Maurice Byukusenge, Tammy Mathews, Traci Pierre, Subhashinie Kariyawasam, Erin Luley, Suresh Kuchipudi, Bhushan Jayarao, Yung-Fu Chang
PLOS ONE.2021; 16(3): e0248528. CrossRef - Short communication: Enterotoxigenic potential of coagulase-negative staphylococci isolated from bovine milk in Poland
I. Helak, E.G. Daczkowska-Kozon, A.A. Dłubała
Journal of Dairy Science.2020; 103(4): 3076. CrossRef -
Comprehensive Virulence Gene Profiling of Bovine Non-
aureus
Staphylococci Based on Whole-Genome Sequencing Data
Sohail Naushad, S. Ali Naqvi, Diego Nobrega, Christopher Luby, John P. Kastelic, Herman W. Barkema, Jeroen De Buck, Angela D. Kent
mSystems.2019;[Epub] CrossRef - Short communication: Search for superantigen genes in coagulase-negative staphylococci isolated from bovine milk in Canada
A. Corbeil, J. Labrie, C. Goetz, S. Dufour, I. Doghri, L. Rivière, M. Jacques
Journal of Dairy Science.2019; 102(3): 2008. CrossRef - Influence of milk, milk fractions and milk proteins on the growth and viability of mastitis-causingStaphylococcus aureusstrain
Karol Fijałkowski, Dorota Peitler, Anna Żywicka, Jolanta Karakulska, Ewa Czerniawska-Piątkowska
Italian Journal of Animal Science.2017; 16(2): 321. CrossRef - Staphylococcus enterotoxin profile of China isolates and the superantigenicity of some novel enterotoxins
Menglu Shen, Yi Li, Linlin Zhang, Songbao Dai, Jiashun Wang, Yongqing Li, Lei Zhang, Jinhai Huang
Archives of Microbiology.2017; 199(5): 723. CrossRef - Relationships among superantigen toxin gene profiles, genotypes, and pathogenic characteristics of Staphylococcus aureus isolates from bovine mastitis
Dong Wang, Limei Zhang, Changfu Yong, Mingliang Shen, Tariq Ali, Muhammad Shahid, Kun Han, Xuezhang Zhou, Bo Han
Journal of Dairy Science.2017; 100(6): 4276. CrossRef - Staphylococci isolated from ready-to-eat meat – Identification, antibiotic resistance and toxin gene profile
Karol Fijałkowski, Dorota Peitler, Jolanta Karakulska
International Journal of Food Microbiology.2016; 238: 113. CrossRef - Antimicrobial susceptibility, virulence genes, and randomly amplified polymorphic DNA analysis of Staphylococcus aureus recovered from bovine mastitis in Ningxia, China
Dong Wang, Limei Zhang, Xuezhang Zhou, Yulong He, Changfu Yong, Mingliang Shen, Otto Szenci, Bo Han
Journal of Dairy Science.2016; 99(12): 9560. CrossRef - First Complete Genome Sequence of Staphylococcus xylosus, a Meat Starter Culture and a Host to Propagate Staphylococcus aureus Phages
Simon J. Labrie, Lynn El Haddad, Denise M. Tremblay, Pier-Luc Plante, Jessica Wasserscheid, Jeannot Dumaresq, Ken Dewar, Jacques Corbeil, Sylvain Moineau
Genome Announcements.2014;[Epub] CrossRef
- Identification and Methicillin Resistance of Coagulase-Negative Staphylococci Isolated from Nasal Cavity of Healthy Horses
-
Jolanta Karakulska , Karol Fijałkowski , Paweł Nawrotek , Anna Pobucewicz , Filip Poszumski , Danuta Czernomysy-Furowicz
-
J. Microbiol. 2012;50(3):444-451. Published online June 30, 2012
-
DOI: https://doi.org/10.1007/s12275-012-1550-6
-
-
693
View
-
0
Download
-
23
Crossref
-
Abstract
PDF
-
The aim of this study was an analysis of the staphylococcal flora of the nasal cavity of 42 healthy horses from 4 farms, along with species identification of CoNS isolates and determination of resistance to 18 antimicrobial agents, particularly phenotypic and genotypic methicillin resistance. From the 81 swabs, 87 staphylococci were isolated. All isolates possessed the gap gene but the coa gene was not detected in any of these isolates. Using PCR-RFLP of the gap gene, 82.8% of CoNS were identified: S. equorum (14.9%), S. warneri (14.9%), S. sciuri (12.6%), S. vitulinus (12.6%), S. xylosus (11.5% ), S. felis (5.7%), S. haemolyticus (3.4%), S. simulans(3.4%), S. capitis (1.1%), S. chromogenes (1.1%), and S. cohnii subsp. urealyticus (1.1%). To our knowledge, this was the first isolation of S. felis from a horse. The species identity of the remaining Staphylococcus spp. isolates (17.2%) could not be determined from the gap gene PCR-RFLP analysis and 16S rRNA gene sequencing data. Based on 16S-23S intergenic transcribed spacer PCR, 11 different ITS-PCR profiles were identified for the 87 analyzed isolates. Results of API Staph were consistent with molecular identification of 17 (19.5%) isolates. Resistance was detected to only 1 or 2 of the 18 antimicrobial agents tested in the 17.2% CoNS isolates, including 6.9% MRCoNS. The mecA gene was detected in each of the 5 (5.7%) phenotypically cefoxitin-resistant isolates and in 12 (13.8%) isolates susceptible to cefoxitin. In total, from 12 horses (28.6%), 17 (19.5%) MRCoNS were isolated. The highest percentage of MRCoNS was noted among S. sciuri isolates (100%).
-
Citations
Citations to this article as recorded by - Characterization of T-cell immune responses against Staphylococcus chromogenes naturally-induced subclinical mastitis in dairy cows
Chen Yu, Jie Wu, Sheng Zhang, Chunmei Ye, Guojun Wei, Zhangping Yang, Zhipeng Zhang
Frontiers in Immunology.2025;[Epub] CrossRef - Analysis of Staphylococcal Diversity in the Skin Microbiota of Healthy Riding Horses
Maria Wesołowska, Ewa Szczuka
Antibiotics.2025; 14(10): 1037. CrossRef - Species diversity, virulence, and antimicrobial resistance of the nasal staphylococcal and mammaliicoccal biota of reindeer
Jolanta Kochel-Karakulska, Marta Maślanko, Marta Woroszyło, Małgorzata Szewczuk, Bartłomiej Grygorcewicz, Karol Fijałkowski
BMC Veterinary Research.2025;[Epub] CrossRef - A One Health Approach Molecular Analysis of Staphylococcus aureus Reveals Distinct Lineages in Isolates from Miranda Donkeys (Equus asinus) and Their Handlers
Vanessa Silva, Cláudia Alfarela, Manuela Caniça, Vera Manageiro, Miguel Nóvoa, Belen Leiva, Maria Kress, José Luís Capelo, Patrícia Poeta, Gilberto Igrejas
Antibiotics.2022; 11(3): 374. CrossRef - Occurrence and spread of antibiotic-resistant bacteria on animal farms and in their vicinity in Poland and Ukraine—review
Karolina Jeżak, Anna Kozajda
Environmental Science and Pollution Research.2022; 29(7): 9533. CrossRef - Identification, Superantigen Toxin Gene Profile and Antimicrobial Resistance of Staphylococci Isolated from Polish Primitive Sheep Breeds
Jolanta Karakulska, Marta Woroszyło, Małgorzata Szewczuk, Karol Fijałkowski
Animals.2022; 12(16): 2139. CrossRef - Comparison between cage and free-range egg production on microbial composition, diversity and the presence of Salmonella enterica
Annaleise Wilson, P. Scott Chandry, Mark S. Turner, Jodi M. Courtice, Narelle Fegan
Food Microbiology.2021; 97: 103754. CrossRef - Nasal colonization and antibiotic resistance patterns of Staphylococcus species isolated from healthy horses in Tripoli, Libya
Aesha A. OTHMAN, Murad A. HIBLU, Mohamed Salah ABBASSI, Yousef M. ABOUZEED, Mohamed O. AHMED
Journal of Equine Science.2021; 32(2): 61. CrossRef - Antimicrobial Resistance of Coagulase-positive Staphylococcus Isolated From Healthy Crioulo Horses and Associated Risk Factors
Sabrina Lopes Mota, Lays Oliveira dos Santos, Marina Roth Vidaletti, Rogério Oliveira Rodrigues, Mario de Menezes Coppola, Fabiana Quoos Mayer
Journal of Equine Veterinary Science.2021; 107: 103779. CrossRef - Multiple Antimicrobial Resistance in Methicillin-Resistant Staphylococcus sciuri Group Isolates from Wild Ungulates in Spain
Joaquín Rey Pérez, Laura Zálama Rosa, Alfredo García Sánchez, Javier Hermoso de Mendoza Salcedo, Juan Manuel Alonso Rodríguez, Rosario Cerrato Horrillo, Sofía Gabriela Zurita, María Gil Molino
Antibiotics.2021; 10(8): 920. CrossRef - Presence and antimicrobial resistance of coagulase-negative staphylococci isolated from animals in a Veterinary Teaching Hospital in Cyprus
H Sukur, OM Esendal
Veterinární medicína.2020; 65(5): 191. CrossRef - Characterization of methicillin-resistance gene mecA in coagulase negative staphylococci (CoNS) recovered from wastewater of two healthcare facilities in Nigeria
Abimbola Olumide Adekanmbi, Olubukola Funmi Soyoye, Olawale Olufemi Adelowo
Gene Reports.2019; 17: 100541. CrossRef - Zoonotic multidrug-resistant microorganisms among non-hospitalized horses from Germany
Ursula Kaspar, Knut von Lützau, Andreas Schlattmann, Uwe Rösler, Robin Köck, Karsten Becker
One Health.2019; 7: 100091. CrossRef - Conjunctival aerobic bacterial flora in healthy Silesian foals and adult horses in Poland
A. Zak, N. Siwinska, M. Slowikowska, H. Borowicz, K. Ploneczka – Janeczko, P. Chorbinski, A. Niedzwiedz
BMC Veterinary Research.2018;[Epub] CrossRef - Silver nanoparticles toxicity against airborne strains ofStaphylococcusspp.
Katarzyna A. Wolny-Koładka, Dagmara K. Malina
Journal of Environmental Science and Health, Part A.2017; 52(13): 1247. CrossRef - Methicillin-resistant coagulase-negative staphylococci in healthy dogs
J. Siugzdaite, A. Gabinaitiene
Veterinární medicína.2017; 62(9): 479. CrossRef - Staphylococcal cassette chromosome mec typing and mecA sequencing in methicillin-resistant staphylococci from Algeria: a highly diversified element with new mutations in mecA
Ferhat Djoudi, Celestino Bonura, Abdelaziz Touati, Aurora Aléo, Said Benallaoua, Caterina Mammina
Journal of Medical Microbiology.2016; 65(11): 1267. CrossRef - Staphylococci isolated from ready-to-eat meat – Identification, antibiotic resistance and toxin gene profile
Karol Fijałkowski, Dorota Peitler, Jolanta Karakulska
International Journal of Food Microbiology.2016; 238: 113. CrossRef - Species determination within Staphylococcus genus by extended PCR-restriction fragment length polymorphism of saoC gene
Michal Bukowski, Klaudia Polakowska, Weronika M. Ilczyszyn, Agnieszka Sitarska, Kinga Nytko, Maja Kosecka, Jacek Miedzobrodzki, Adam Dubin, Benedykt Wladyka
FEMS Microbiology Letters.2015; 362(1): 1. CrossRef - The ecological importance of the Staphylococcus sciuri species group as a reservoir for resistance and virulence genes
Stéphanie Nemeghaire, M. Angeles Argudín, Andrea T. Feßler, Tomasz Hauschild, Stefan Schwarz, Patrick Butaye
Veterinary Microbiology.2014; 171(3-4): 342. CrossRef - Could Public Restrooms Be an Environment for Bacterial Resistomes?
Hermine V. Mkrtchyan, Charlotte A. Russell, Nan Wang, Ronald R. Cutler, Patrick M. Schlievert
PLoS ONE.2013; 8(1): e54223. CrossRef - Clinical and molecular features of methicillin-resistant, coagulase-negative staphylococci of pets and horses
A. Kern, V. Perreten
Journal of Antimicrobial Chemotherapy.2013; 68(6): 1256. CrossRef - Methicillin resistant staphylococci and broad-spectrum β-lactamase producing Enterobacteriaceae in horses
Filip Boyen, Annemieke Smet, Katleen Hermans, Patrick Butaye, Ann Martens, An Martel, Freddy Haesebrouck
Veterinary Microbiology.2013; 167(1-2): 67. CrossRef
|
MSK
