- Lipocalin2 as a potential antibacterial drug against Acinetobacter baumannii infection
-
Daejin Lim , Su-Jin Park , Ha Young Kim , Minsang Shin , Miryoung Song
-
J. Microbiol. 2022;60(4):444-449. Published online March 28, 2022
-
DOI: https://doi.org/10.1007/s12275-022-2007-1
-
-
1,069
View
-
8
Download
-
4
Web of Science
-
4
Crossref
-
 Abstract
 PDF
-
Available antibiotics to treat Acinetobacter baumannii infection
is limited due to increasing resistance and the emergence
of multiple drug-resistant strains. Hence, discovering effective
agents against A. baumannii to reduce the number of infectionrelated
deaths is imperative. In search of novel and alternative
antibiotics, the antibacterial function of lipocalin2 (Lcn2) was
investigated to treat systemic infections of A. baumannii using
a mouse neutropenia model. We observed a significant increase
in serum Lcn2 levels upon bacterial injection into the
mouse, and the administration of recombinant Lcn2 (rmLcn2)
extended their survival. Such protective effects were also observed
in rmLcn2-pretreated macrophages, where rmLcn2
reduced the survival of the pathogen inside the macrophages.
The underlying molecular mechanism of Lcn2 protection was
also investigated. We observed that pretreatment of the Raw-
264.7 macrophages with rmLcn2 markedly altered the expression
of tonB3, which encodes a component of the transporter
for ferrisiderophores in A. baumannii. However, the
expression of katG, the gene encoding catalase, remained unaffected.
These indicate that Lcn2-mediated defense against
the pathogen is related to nutritional immunity rather than
reactive oxygen species (ROS) production. Furthermore, the
addition of rmLcn2 in infected mice diminished bacterial burden
in multiple organs and enhanced the expression of tonB3
in the liver, spleen, and lungs of the infected mice. Increased
survival rate due to rmLcn2 treatment declined when the infection
model was established using lcn2-defective (lcn2-/-)
mice, which indicated the necessity of endogenous Lcn2. Therefore,
the antibacterial function of Lcn2 can be exploited to
develop an alternative therapeutic agent against A. baumannii.
-
Citations
Citations to this article as recorded by  - Liver as a key organ for systemic antimicrobial defense
Tiantian Yao, Luca Maccioni, Yukun Guan, Robim M. Rodrigues, Bin Gao
Hepatology Communications.2025;[Epub] CrossRef - Antimicrobial peptide thanatin fused endolysin PA90 (Tha-PA90) for the control of Acinetobacter baumannii infection in mouse model
Jeonghyun Lim, Heejoon Myung, Daejin Lim, Miryoung Song
Journal of Biomedical Science.2024;[Epub] CrossRef - Dynamic changes and clinical value of lipocalin 2 in liver diseases caused by microbial infections
Feng Chen, Shan-Shan Wu, Chao Chen, Cheng Zhou
World Journal of Hepatology.2024; 16(2): 177. CrossRef - Lipocalin-2 is an essential component of the innate immune response to Acinetobacter baumannii infection
Jessica R. Sheldon, Lauren E. Himmel, Dillon E. Kunkle, Andrew J. Monteith, K. Nichole Maloney, Eric P. Skaar, David S. Weiss
PLOS Pathogens.2022; 18(9): e1010809. CrossRef
- Development of bacteria as diagnostics and therapeutics by genetic engineering
-
Daejin Lim , Miryoung Song
-
J. Microbiol. 2019;57(8):637-643. Published online May 11, 2019
-
DOI: https://doi.org/10.1007/s12275-019-9105-8
-
-
1,042
View
-
1
Download
-
17
Web of Science
-
18
Crossref
-
Abstract
PDF
-
Bacteria sense and respond to the environment, communicate,
and continuously interact with their surroundings, including
host bodies. For more than a century, engineers have been
trying to harness the natural ability of bacteria as live biotherapeutics
for the treatment of diseases. Recent advances in synthetic
biology facilitate the enlargement of the repertoire of
genetic parts, tools, and devices that serve as a framework for
biotherapy. This review describes bacterial species developed
for specific diseases shown in in vitro studies and clinical stages.
Here, we focus on drug delivery by programing bacteria and
discuss the challenges for safety and improvement.
-
Citations
Citations to this article as recorded by - Bacteriobots: Bacterial Microrobots and Their Potential in Cancer Diagnosis and Therapy
L.N. Ikryannikova
Biomedical Chemistry: Research and Methods.2026; 9(1): e00301. CrossRef - Modified probiotics and the related combinatorial therapeutics
Luo Zhao, Mengya Niu, Zilin Ma, Fengyun He, Xinxin Liu, Xunwei Gong, Zhanfei Chai, Ziqing Wang, Qianhua Feng, Lei Wang
Acta Pharmaceutica Sinica B.2025; 15(5): 2431. CrossRef - Applications of Nanomaterial‐Microorganism Hybrid Systems in the Treatment of Tumor
Muskan Saif Khan, Mirza Albash Baig, Meng Tian, Bowen Li, Guoqing Feng, Run Yang, Yang Bai, Bin Zheng
Advanced Therapeutics.2025;[Epub] CrossRef - Genetically engineered bacteria expressing IL-34 alleviate DSS-induced experimental colitis by promoting tight junction protein expression in intestinal mucosal epithelial cells
Weijie Chen, Tongtong Zhou, Yicun Liu, Leilei Luo, Yujing Ye, Lixian Wei, Jian Chen, Zhaolian Bian
Molecular Immunology.2025; 178: 64. CrossRef - Surface modification of probiotics at single-cell resolution: Strategies, applications, and future directions in inflammatory bowel disease
Li Peng, Xinyu Wang, Yafen Wang, Jueshuo Guo, Jianhong Yang
Materials Today Bio.2025; 35: 102485. CrossRef - Engineered Microorganisms for Advancing Tumor Therapy
Jinxuan Jia, Xiaocheng Wang, Xiang Lin, Yuanjin Zhao
Advanced Materials.2024;[Epub] CrossRef - Therapeutic bacteria and viruses to combat cancer: double-edged sword in cancer therapy: new insights for future
Aref Yarahmadi, Mitra Zare, Masoomeh Aghayari, Hamed Afkhami, Gholam Ali Jafari
Cell Communication and Signaling.2024;[Epub] CrossRef - Physiochemically and Genetically Engineered Bacteria: Instructive Design Principles and Diverse Applications
Xia Lin, Rong Jiao, Haowen Cui, Xuebing Yan, Kun Zhang
Advanced Science.2024;[Epub] CrossRef - Intestinal Delivery of Probiotics: Materials, Strategies, and Applications
Chengcheng Li, Zi‐Xi Wang, Huining Xiao, Fu‐Gen Wu
Advanced Materials.2024;[Epub] CrossRef - Research and application of intelligent diagnosis and treatment engineering bacteria
Na Zhao, Junwei Chen, Jingtian Shi, Yan Gao, Lijing Li, Liyun Dong
Frontiers in Bioengineering and Biotechnology.2024;[Epub] CrossRef - Gastrointestinal worms and bacteria: From association to intervention
James Rooney, Cinzia Cantacessi, Javier Sotillo, Alba Cortés
Parasite Immunology.2023;[Epub] CrossRef - Bacterial Therapy of Cancer: A Way to the Dustbin of History or to the Medicine of the Future?
Larisa N. Ikryannikova, Neonila V. Gorokhovets, Darya A. Belykh, Leonid K. Kurbatov, Andrey A. Zamyatnin
International Journal of Molecular Sciences.2023; 24(11): 9726. CrossRef - Derivation and elimination of uremic toxins from kidney-gut axis
Ying Xu, Wen-Di Bi, Yu-Xuan Shi, Xin-Rui Liang, Hai-Yan Wang, Xue-Li Lai, Xiao-Lu Bian, Zhi-Yong Guo
Frontiers in Physiology.2023;[Epub] CrossRef - Decorated bacteria and the application in drug delivery
Feng Wu, Jinyao Liu
Advanced Drug Delivery Reviews.2022; 188: 114443. CrossRef - Bakterie Modyfikowane Genetycznie – Perspektywy Zastosowania w Profilaktyce, Diagnostyce I Terapii
Barbara Macura, Aneta Kiecka, Marian Szczepanik
Postępy Mikrobiologii - Advancements of Microbiology.2022; 61(1): 21. CrossRef - Bacteria and cells as alternative nano-carriers for biomedical applications
Rafaela García-Álvarez, María Vallet-Regí
Expert Opinion on Drug Delivery.2022; 19(1): 103. CrossRef -
Tabrizicola piscis sp. nov., isolated from the intestinal tract of a Korean indigenous freshwater fish, Acheilognathus koreensis
Jeong Eun Han, Woorim Kang, June-Young Lee, Hojun Sung, Dong-Wook Hyun, Hyun Sik Kim, Pil Soo Kim, Euon Jung Tak, Yun-Seok Jeong, Jae-Yun Lee, So-Yeon Lee, Ji-Hyun Yun, Mi-Ja Jung, Na-Ri Shin, Tae Woong Whon, Myung-Suk Kang, Ki-Eun Lee, Byoung-Hee Lee, Ji
International Journal of Systematic and Evolutionary Microbiology
.2020; 70(4): 2305. CrossRef - Novel Strategies for Efficient Production and Delivery of Live Biotherapeutics and Biotechnological Uses of Lactococcus lactis: The Lactic Acid Bacterium Model
Laísa M. Tavares, Luís C. L. de Jesus, Tales F. da Silva, Fernanda A. L. Barroso, Viviane L. Batista, Nina D. Coelho-Rocha, Vasco Azevedo, Mariana M. Drumond, Pamela Mancha-Agresti
Frontiers in Bioengineering and Biotechnology.2020;[Epub] CrossRef
|
MSK
