Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search

Author index

Page Path
HOME > Browse Articles > Author index
Search
Chul-Joong Kim 6 Articles
Inhibitory effects of bee venom and its components against viruses in vitro and in vivo
Md Bashir Uddin , Byeong-Hoon Lee , Chamilani Nikapitiya , Jae-Hoon Kim , Tae-Hwan Kim , Hyun-Cheol Lee , Choul Goo Kim , Jong-Soo Lee , Chul-Joong Kim
J. Microbiol. 2017;55(2):160-160.
DOI: https://doi.org/10.1007/s12275-017-0668-y
  • 604 View
  • 0 Download
AbstractAbstract PDF
In the article by Uddin et al. published in Journal of Microbiology 2016; 54, 853–866, the Protocol number on page 856 and 865 should be corrected as below.
Inhibitory effects of bee venom and its components against viruses in vitro and in vivo
Md Bashir Uddin , Byeong-Hoon Lee , Chamilani Nikapitiya , Jae-Hoon Kim , Tae-Hwan Kim , Hyun-Cheol Lee , Choul Goo Kim , Jong-Soo Lee , Chul-Joong Kim
J. Microbiol. 2016;54(12):853-866.   Published online November 26, 2016
DOI: https://doi.org/10.1007/s12275-016-6376-1
  • 1,056 View
  • 0 Download
  • 104 Crossref
AbstractAbstract PDF
Bee venom (BV) from honey bee (Apis Melifera L.) contains at least 18 pharmacologically active components including melittin (MLT), phospholipase A2 (PLA2), and apamin etc. BV is safe for human treatments dose dependently and proven to possess different healing properties including antibacterial and antiparasitidal properties. Nevertheless, antiviral properties of BV have not well investigated. Hence, we identified the potential antiviral properties of BV and its component against a broad panel of viruses. Co-incubation of non-cytotoxic amounts of BV and MLT, the main component of BV, significantly inhibited the replication of enveloped viruses such as Influenza A virus (PR8), Vesicular Stomatitis Virus (VSV), Respiratory Syncytial Virus (RSV), and Herpes Simplex Virus (HSV). Additionally, BV and MLT also inhibited the replication of non-enveloped viruses such as Enterovirus-71 (EV-71) and Coxsackie Virus (H3). Such antiviral properties were mainly explained by virucidal mechanism. Moreover, MLT protected mice which were challenged with lethal doses of pathogenic influenza A H1N1 viruses. Therefore, these results provides the evidence that BV and MLT could be a potential source as a promising antiviral agent, especially to develop as a broad spectrum antiviral agent.

Citations

Citations to this article as recorded by  
  • Bee products as alternatives in the treatment of viral infections
    Michał Otręba, Łukasz Marek, Jerzy Stojko, Anna Rzepecka‐Stojko
    Journal of the Science of Food and Agriculture.2026; 106(1): 33.     CrossRef
  • Screening and Identification of TRPV1-Targeting Peptides from Apis mellifera Venom with Inflammatory Modulatory Potential
    Kai Wang, Jiangtao Qiao, Chao Ji, Eric Haubruge, Gauthier Eppe, Xianbin Meng, Christopher Kune, Loïc Quinton, Hongcheng Zhang
    Journal of Natural Products.2026; 89(4): 1174.     CrossRef
  • Synergistic Antiviral Effect of Bee Venom and Chitosan Nanoparticles Obtained from Chrysomya albiceps Maggots as a Natural Combination: In vitro and in silico Studies
    Reda H. Elmorsy, Mostafa I. Hassan, Mohammad R. K. Abdel-Samad
    Molecular Biotechnology.2026; 68(6): 2941.     CrossRef
  • From Hive to Health: Anti‐Influenza Properties of Bee Products
    Wei Wei Loo, Hien Tet Lai, Ji Wei Tan, Elena Sorrentino
    International Journal of Microbiology.2026;[Epub]     CrossRef
  • Discovery of a novel compound against BmNPV using virtual screening based on the protein structure of the viral whole genome
    Lin Zhang, Ying Ma, Junchi Hu, Feifei Liu, Yunxiang Jia, Peng Chen, Adrian Oo, Zhanqi Dong, Minhui Pan
    Pesticide Biochemistry and Physiology.2026; 219: 107031.     CrossRef
  • BBB modulation-driven potentiation of antifungal therapy by melittin in fungal encephalitis
    Mingsheng Li, Jing Ai, Chengwei Zhang, Qing Zhang, Shuze Li, Hongying Jin, Xiang Yu
    International Immunopharmacology.2026; 179: 116633.     CrossRef
  • Bee Venom and Its Therapeutic Potential
    Betül Su Doğan, Hande Sultan Şahiner
    Animal Health Production and Hygiene.2026;[Epub]     CrossRef
  • Nanocarrier-Enabled Melittin Therapy: Mechanistic Advances, Therapeutic Applications, and Translational Challenges
    Yuezhihong Xiong, Huili Cai, Siyu Jia, Boya Gong, Kairong Hou, Hongyan Wu, Haidan Chen
    International Journal of Nanomedicine.2026; Volume 21: 1.     CrossRef
  • Potential Effects of Bee Products Against Hantavirus Infection: Potential Mechanisms of Action and Future Directions
    Saad N. Al-Kahtani, Ahmed A. Rawwash, Amal Semmar, Sahar Gaber, Nabil M. Elwakeil, El-Kazafy A. Taha
    Life.2026; 16(6): 995.     CrossRef
  • Veteriner Hekimlik Alanında Bal, Propolis ve Arı Zehirinin Terapötik Amaçlı Kullanım Potansiyelleri
    Ceyda Gökçe Vurgun
    Manas Journal of Agriculture Veterinary and Life Sciences.2026; 16(1): 171.     CrossRef
  • Insight into cytotoxic and molecular mechanisms of bee venom and its nanoemulsion against West Nile virus in vitro
    Abeer M. Salem, Heba Seyam, Sameh Ismail, Mohammed A. Abd El-Mobdy, Eman E. Zaher, El-Sayed H. Shaurub, Soad A. ElKenawi
    Scientific Reports.2026;[Epub]     CrossRef
  • An in-depth review on antimicrobial efficacy of melittin in vivo
    Hamed Memariani, Mojtaba Memariani, Mohammadali Mazloumi
    World Journal of Microbiology and Biotechnology.2026;[Epub]     CrossRef
  • Potential of Venom-Derived Compounds for the Development of New Antimicrobial Agents
    Esraa Yasser Rabea, Esraa Dakrory Mahmoud, Nada Khaled Mohamed, Erada Rabea Ansary, Mahmoud Roushdy Alrouby, Rabab Reda Shehata, Youssef Yasser Mokhtar, Prakash Arullampalam, Ahmed M. Hegazy, Ahmed Al-Sabi, Tarek Mohamed Abd El-Aziz
    Toxins.2025; 17(5): 238.     CrossRef
  • Bothropstoxins I and II as potent phospholipase A2 molecules from Bothrops jararacussu to impair hepatitis C virus infection
    Carina Machado Pereira, Jacqueline Farinha Shimizu, Natasha Marques Cassani, Igor Andrade Santos, Cintia Bittar, Adélia Cristina Oliveira Cintra, Suely Vilela Sampaio, Mark Harris, Paula Rahal, Ana Carolina Gomes Jardim
    Biochimie.2025; 235: 39.     CrossRef
  • Recent Advances in Therapeutic Peptides: Innovations and Applications in Treating Infections and Diseases
    Deepshikha Sharma, Isha Dhiman, Swarnali Das, Deepak Kumar Das, Devlina Das Pramanik, Sandeep Kumar Dash, Arindam Pramanik
    ACS Omega.2025; 10(17): 17087.     CrossRef
  • Advancements in antiviral approaches against foot-and-mouth disease virus: a comprehensive review
    Mahmoud Mohamadin, Rashid Manzoor, Ahmed Elolimy, Mohamed Abdelmegeid, Samah Mosad, Sahar Abd El Rahman
    Frontiers in Veterinary Science.2025;[Epub]     CrossRef
  • Antimicrobial Potential of Bee-Derived Products: Insights into Honey, Propolis and Bee Venom
    Agnieszka Grinn-Gofroń, Maciej Kołodziejczak, Rafał Hrynkiewicz, Filip Lewandowski, Dominika Bębnowska, Cezary Adamski, Paulina Niedźwiedzka-Rystwej
    Pathogens.2025; 14(8): 780.     CrossRef
  • Therapeutic Bioactivity Exerted by the Apis mellifera Bee Venom and Its Major Protein Melittin: A Scoping Review
    Perihan Mutlu Erdoğan, Funda Bilgili-Tetikoğlu, Selcen Çelik-Uzuner, Oktay Yıldız, Sevgi Kolayli, Dimitris Mossialos
    Molecules.2025; 30(19): 4003.     CrossRef
  • PLGA nanoparticles provide a safe delivery system for bee venom and melittin to alleviate their hepatotoxic effects in mice
    Asmaa M. Shehata, Hala F. Aref, Mohamed Y. Mahmoud, Alaa F. Bakr, Maha A. Salem, Eiman M. El-Saied
    Toxicon.2025; 267: 108564.     CrossRef
  • Apitoxin to apitherapy: a review of bee venom and therapeutic applications
    Mohammed M. Abdel-Monsef, Hassan M.M. Masoud, Doaa A. Darwish, Mohamed S. Helmy, Hind A. Zidan, Sayed S. Esa
    Egyptian Journal of Basic and Applied Sciences.2025; 12(1): 485.     CrossRef
  • CAPRİPOXVİRUSLARIN HAYVAN SAĞLIĞI ÜZERİNDEKİ ETKİLERİ VE GÜNCEL ANTİVİRAL YAKLAŞIMLAR
    Sibel Şurak, Zeynep Akkutay Yoldar
    Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni.2024; 15(2): 86.     CrossRef
  • Mechanisms of Action, Biological Characteristics, and Future Prospects: A Review of Antimicrobial Peptides (A Review)
    X. Ma, Z. Chen, M. Long
    Applied Biochemistry and Microbiology.2024; 60(6): 1044.     CrossRef
  • Structural characterization and bioactivity profiling of the fungal peptaibiotic tolypin reveal protective effects against influenza viruses
    Johanna Eichberg, Markus Oberpaul, Christoph Hartwig, Andrea Helga Geißler, Carsten Culmsee, Andreas Vilcinskas, Eva Böttcher‐Friebertshäuser, Hans Brückner, Thomas Degenkolb, Kornelia Hardes
    Archiv der Pharmazie.2024;[Epub]     CrossRef
  • Antiviral Activities of Mastoparan-L-Derived Peptides against Human Alphaherpesvirus 1
    Liana Costa Pereira Vilas Boas, Danieli Fernanda Buccini, Rhayfa Lorrayne Araújo Berlanda, Bruno de Paula Oliveira Santos, Mariana Rocha Maximiano, Luciano Morais Lião, Sónia Gonçalves, Nuno C. Santos, Octávio Luiz Franco
    Viruses.2024; 16(6): 948.     CrossRef
  • Anti-Herpes Simplex Virus and Anti-Inflammatory Activities of the Melittin Peptides Derived from Apis mellifera and Apis florea Venom
    Pichet Praphawilai, Thida Kaewkod, Sureeporn Suriyaprom, Aussara Panya, Terd Disayathanoowat, Yingmanee Tragoolpua
    Insects.2024; 15(2): 109.     CrossRef
  • Melittin as a therapeutic agent for rheumatoid arthritis: mechanistic insights, advanced delivery systems, and future perspectives
    Ashutosh Pareek, Khushbu Mehlawat, Kritika Tripathi, Aaushi Pareek, Simran Chaudhary, Yashumati Ratan, Vasso Apostolopoulos, Anil Chuturgoon
    Frontiers in Immunology.2024;[Epub]     CrossRef
  • A Journey Along the Boulevard of Bioactive Compounds from Natural Sources, with Cosmetic and Pharmaceutical Potential: Bee Venom, Cobra Venom, Ficus carica
    Monica Dinu, Carmen Galea, Ana Maria Chirilov, Alin Laurențiu Tatu, Lawrence Chukwudi Nwabudike, Olimpia Dumitriu Buzia, Claudia Simona Stefan
    Cosmetics.2024; 11(6): 195.     CrossRef
  • Bee-Inspired Healing: Apitherapy in Veterinary Medicine for Maintenance and Improvement Animal Health and Well-Being
    Jevrosima Stevanović, Uroš Glavinić, Marko Ristanić, Vladimira Erjavec, Barış Denk, Slobodan Dolašević, Zoran Stanimirović
    Pharmaceuticals.2024; 17(8): 1050.     CrossRef
  • The current landscape of the antimicrobial peptide melittin and its therapeutic potential
    Hai-Qian Zhang, Chengbiao Sun, Na Xu, Wensen Liu
    Frontiers in Immunology.2024;[Epub]     CrossRef
  • Immunomodulatory activities and biomedical applications of melittin and its recent advances
    Zohreh Jafari, Sahar Sadeghi, Mahsa Mirzarazi Dehaghi, Ashkan Bigham, Shokouh Honarmand, Afsaneh Tavasoli, Mostafa Haji Molla Hoseini, Rajender S. Varma
    Archiv der Pharmazie.2024;[Epub]     CrossRef
  • Bee venom and melittin: Potent key enzyme inhibitors with promising therapeutic potential
    Bayram Alparslan, Murat Şentürk, Cengiz Erkan
    Toxicon.2024; 252: 108164.     CrossRef
  • Expression profiles and phylogenetic properties of venom gland‐specific viruses in some aculeate bees and wasps
    Kyungjae Andrew Yoon, Woo Jin Kim, Hee Jong Shin, Si Hyeock Lee
    Entomological Research.2024;[Epub]     CrossRef
  • DOĞANIN ŞİFASI: ARI ZEHRİNİN SAĞLIK ÜZERİNDEKİ ETKİLERİ VE UYGULAMALARI
    Sibel Kaymak, Nilüfer Vural, Oğuz Yüce, Salih Mollahaliloğlu
    Uludağ Arıcılık Dergisi.2024; 24(2): 386.     CrossRef
  • Plantar warts successfully treated with bee venom: A case report
    Min Hee Kim
    EXPLORE.2024; 20(2): 231.     CrossRef
  • Bee venom: apitherapy and more
    Maya JODIDIO, Robert A. SCHWARTZ
    Italian Journal of Dermatology and Venereology.2024;[Epub]     CrossRef
  • Harnessing the power of bee venom for therapeutic and regenerative medical applications: an updated review
    Kadry M. Sadek, Naira A. Shib, Ehab S. Taher, Fatema Rashed, Mustafa Shukry, Gamal A. Atia, Noha Taymour, Mohammad El-Nablaway, Ateya M. Ibrahim, Mahmoud M. Ramadan, Afaf Abdelkader, Mohamed Abdo, Ilinca Imbrea, Elena Pet, Lashin S. Ali, Ahmed Abdeen
    Frontiers in Pharmacology.2024;[Epub]     CrossRef
  • Cytotoxic and apoptotic effectiveness of Cypriot honeybee (Apis mellifera cypria) venom on various cancer cells
    Ayşe Nalbantsoy, Ekin Varol, Ayşe Dila Çaglar, Banu Yücel
    Turkish Journal of Biochemistry.2024; 49(3): 446.     CrossRef
  • Orchestrating AMPK/mTOR signaling to initiate melittin-induced mitophagy: A neuroprotective strategy against Parkinson's disease
    Mingran Chen, Xue Wang, Shuangyan Bao, Dexiao Wang, Jie Zhao, Qian Wang, Chaojie Liu, Haiong Zhao, Chenggui Zhang
    International Journal of Biological Macromolecules.2024; 281: 136119.     CrossRef
  • Bee Venom and Its Therapeutic Uses
    Hassan Morovvati, Haydeh Keyhan, Mohammad Kazem Koohi, Jalal Hassan
    Journal of Apitherapy and Nature.2024; 7(2): 65.     CrossRef
  • A potential antifungal bioproduct for Microsporum canis: Bee venom
    Armağan E. Ütük, Tülin Güven Gökmen, Hatice Yazgan, Funda Eşki, Nevin Turut, Şifa Karahan, İbrahim Kıvrak, Sedat Sevin, Osman Sezer
    Onderstepoort Journal of Veterinary Research.2024;[Epub]     CrossRef
  • Insights into enterovirus a-71 antiviral development: from natural sources to synthetic nanoparticles
    Shiraz Feferbaum-Leite, Igor Andrade Santos, Victória Riquena Grosche, Gislaine Celestino Dutra da Silva, Ana Carolina Gomes Jardim
    Archives of Microbiology.2023;[Epub]     CrossRef
  • Can Bee Venom Be Used as Anticancer Agent in Modern Medicine?
    Agata Małek, Maciej Strzemski, Joanna Kurzepa, Jacek Kurzepa
    Cancers.2023; 15(14): 3714.     CrossRef
  • Bee Venom Induces the Interaction between Phosphorylated Histone Variant, H2AX, and the Intracellular Site of beta‐Actin in Liver and Breast Cancer Cells
    Sinan Tetikoğlu, Selcen Çelik‐Uzuner
    Chemistry & Biodiversity.2023;[Epub]     CrossRef
  • In-Ovo injection of melittin into Alexandria chicken eggs: a way for early immune acceleration
    Mohamed H. Khalil, Saber S. Hassan, Farid N. K. Soliman, Mohamed I. Hassan
    Animal Biotechnology.2023; 34(8): 4060.     CrossRef
  • Determination of the Effects of Bee Venom on Triple Negative Breast Cancer Cells in Vitro
    Sedat Sevin, Asuman Deveci Ozkan, Hidayet Tutun, Ibrahim Kivrak, Ozge Turna, Gamze Guney Eskiler
    Chemistry & Biodiversity.2023;[Epub]     CrossRef
  • Experimental envenomation with honeybee venom melittin and phospholipase A2 induced multiple ultrastructural changes in adrenocortical mitochondria
    Diana Harfmann, Adrian Florea
    Toxicon.2023; 229: 107136.     CrossRef
  • Bee Venom and Its Two Main Components—Melittin and Phospholipase A2—As Promising Antiviral Drug Candidates
    Carole Yaacoub, Rim Wehbe, Rabih Roufayel, Ziad Fajloun, Bruno Coutard
    Pathogens.2023; 12(11): 1354.     CrossRef
  • Synthesis of bee venom loaded chitosan nanoparticles for anti-MERS-COV and multi-drug resistance bacteria
    Mohamed E. Elnosary, Hesham A. Aboelmagd, Manal A. Habaka, Salem R. Salem, Mehrez E. El-Naggar
    International Journal of Biological Macromolecules.2023; 224: 871.     CrossRef
  • Melittin-Related Peptides Interfere with Sandfly Fever Naples Virus Infection by Interacting with Heparan Sulphate
    Annalisa Chianese, Carla Zannella, Francesca Palma, Laura Di Clemente, Alessandra Monti, Nunzianna Doti, Anna De Filippis, Massimiliano Galdiero
    Microorganisms.2023; 11(10): 2446.     CrossRef
  • Therapeutic Use of Bee Venom and Potential Applications in Veterinary Medicine
    Roberto Bava, Fabio Castagna, Vincenzo Musella, Carmine Lupia, Ernesto Palma, Domenico Britti
    Veterinary Sciences.2023; 10(2): 119.     CrossRef
  • Antiviral Attributes of Bee Venom as a Possible Therapeutic Approach Against SARS-CoV-2 Infection
    Soumik Goswami, Jayita Pal Chowdhury
    Future Virology.2023; 18(15): 1021.     CrossRef
  • Antimicrobial Activity of Apis mellifera Bee Venom Collected in Northern Peru
    Orlando Pérez-Delgado, Abraham Omar Espinoza-Culupú, Elmer López-López
    Antibiotics.2023; 12(4): 779.     CrossRef
  • General Nutritional Profile of Bee Products and Their Potential Antiviral Properties against Mammalian Viruses
    Syeda Tasmia Asma, Otilia Bobiş, Victoriţa Bonta, Ulas Acaroz, Syed Rizwan Ali Shah, Fatih Ramazan Istanbullugil, Damla Arslan-Acaroz
    Nutrients.2022; 14(17): 3579.     CrossRef
  • THERAPEUTICS APPROACHES OF INVERTEBRATE ANIMAL TOXINS: A REVIEW
    SIMRAN SHARMA, RAVI KANT UPADHYAY
    International Journal of Pharmacy and Pharmaceutical Sciences.2022; : 8.     CrossRef
  • In Silico Analysis of Honeybee Venom Protein Interaction with Wild Type and Mutant (A82V + P375S) Ebola Virus Spike Protein
    Muhammad Muzammal, Muzammil Khan, Mohammed Mohaini, Abdulkhaliq Alsalman, Maitham Hawaj, Arshad Farid
    Biologics.2022; 2(1): 45.     CrossRef
  • Antiviral Effects of Animal Toxins: Is There a Way to Drugs?
    Yuri Utkin, Andrei Siniavin, Igor Kasheverov, Victor Tsetlin
    International Journal of Molecular Sciences.2022; 23(7): 3634.     CrossRef
  • Antiviral Activity of Bee Products
    Theodoros Kontogiannis, Tilemachos G. Dimitriou, Nikos Asoutis Didaras, Dimitris Mossialos
    Current Pharmaceutical Design.2022; 28(35): 2867.     CrossRef
  • Animal venoms as a source of antiviral peptides active against arboviruses: a systematic review
    William Gustavo Lima, César Quadros Maia, Thayane Santos de Carvalho, Gustavo Oliveira Leite, Júlio César Moreira Brito, Isabella Piassi Dias Godói, Maria Elena de Lima, Jaqueline Maria Siqueira Ferreira
    Archives of Virology.2022; 167(9): 1763.     CrossRef
  • Reptiles as Promising Sources of Medicinal Natural Products for Cancer Therapeutic Drugs
    Soon Yong Park, Hyeongrok Choi, Jin Woong Chung
    Pharmaceutics.2022; 14(4): 874.     CrossRef
  • Antiviral Potential of Natural Resources against Influenza Virus Infections
    Johanna Eichberg, Elena Maiworm, Markus Oberpaul, Volker Czudai-Matwich, Tim Lüddecke, Andreas Vilcinskas, Kornelia Hardes
    Viruses.2022; 14(11): 2452.     CrossRef
  • Effect of honey bee venom on the histological changes of testes and hormonal disturbance in diabetic mice
    Sattar J. J. AL-Shaeli, Talal Jabal Hussen, Ali M. Ethaeb
    Veterinary World.2022; : 2357.     CrossRef
  • Gramicidin S and melittin: potential anti-viral therapeutic peptides to treat SARS-CoV-2 infection
    Mohammed Ghalib Enayathullah, Yash Parekh, Sarena Banu, Sushma Ram, Ramakrishnan Nagaraj, Bokara Kiran Kumar, Mohammed M. Idris
    Scientific Reports.2022;[Epub]     CrossRef
  • Anti-fungal Effects and Mechanisms of Action of Wasp Venom-Derived Peptide Mastoparan-VT1 Against Candida albicans
    Mojtaba Memariani, Hamed Memariani, Zahra Poursafavi, Zohre Baseri
    International Journal of Peptide Research and Therapeutics.2022;[Epub]     CrossRef
  • Melittin-Based Nano-Delivery Systems for Cancer Therapy
    Anqi Wang, Yuan Zheng, Wanxin Zhu, Liuxin Yang, Yang Yang, Jinliang Peng
    Biomolecules.2022; 12(1): 118.     CrossRef
  • New Insights into Potential Beneficial Effects of Bioactive Compounds of Bee Products in Boosting Immunity to Fight COVID-19 Pandemic: Focus on Zinc and Polyphenols
    Meryem Bakour, Hassan Laaroussi, Driss Ousaaid, Asmae El Ghouizi, Imane Es-safi, Hamza Mechchate, Badiaa Lyoussi
    Nutrients.2022; 14(5): 942.     CrossRef
  • RETRACTED ARTICLE: COVID-19 pandemic: impacts on bees, beekeeping, and potential role of bee products as antiviral agents and immune enhancers
    Youssef A. Attia, Gianpaolo M. Giorgio, Nicola F. Addeo, Khalid A. Asiry, Giovanni Piccolo, Antonino Nizza, Carmelo Di Meo, Naimah A. Alanazi, Adel D. Al-qurashi, Mohamed E. Abd El-Hack, Asmaa F. Khafaga, Fulvia Bovera
    Environmental Science and Pollution Research.2022; 29(7): 9592.     CrossRef
  • The Broad-Spectrum Antiviral Potential of the Amphibian Peptide AR-23
    Annalisa Chianese, Carla Zannella, Alessandra Monti, Anna De Filippis, Nunzianna Doti, Gianluigi Franci, Massimiliano Galdiero
    International Journal of Molecular Sciences.2022; 23(2): 883.     CrossRef
  • Exploring the potential therapeutic properties of bee products against COVID-19 (SARS CoV-2)
    S. Sen, S. Borkataki, R.R. Taye, S.P. Nanda, M.D. Reddy
    Journal of Entomological Research.2022; 46(4): 869.     CrossRef
  • Pharmaceutical Prospects of Bee Products: Special Focus on Anticancer, Antibacterial, Antiviral, and Antiparasitic Properties
    Firzan Nainu, Ayu Masyita, Muh. Akbar Bahar, Muhammad Raihan, Shajuthi Rahman Prova, Saikat Mitra, Talha Bin Emran, Jesus Simal-Gandara
    Antibiotics.2021; 10(7): 822.     CrossRef
  • Bee Venom: From Venom to Drug
    Abdelwahab Khalil, Basem H. Elesawy, Tarek M. Ali, Osama M. Ahmed
    Molecules.2021; 26(16): 4941.     CrossRef
  • The Responsiveness of Bee Venom Phospholipase A2 on Regulatory T Cells Correlates with the CD11c+CD206+Population in Human Peripheral Blood Mononuclear Cells
    Heejin Jo, Hyunjung Baek, Seon-Young Park, Bonhyuk Goo, Woo-Sang Jung, Hyunsu Bae, Sang-Soo Nam
    Toxins.2021; 13(10): 717.     CrossRef
  • Antiviral peptides against Enterovirus A71 causing hand, foot and mouth disease
    Salima Lalani, Lai Ti Gew, Chit Laa Poh
    Peptides.2021; 136: 170443.     CrossRef
  • Mechanism of antimicrobial activity of honeybee (Apis mellifera) venom on Gram-negative bacteria: Escherichia coli and Pseudomonas spp.
    Izlem Haktanir, Maria Masoura, Fani Th Mantzouridou, Konstantinos Gkatzionis
    AMB Express.2021;[Epub]     CrossRef
  • Applications and evolution of melittin, the quintessential membrane active peptide
    Shantanu Guha, Ryan P. Ferrie, Jenisha Ghimire, Cristina R. Ventura, Eric Wu, Leisheng Sun, Sarah Y. Kim, Gregory R. Wiedman, Kalina Hristova, Wimley C. Wimley
    Biochemical Pharmacology.2021; 193: 114769.     CrossRef
  • Naturally-derived targeted therapy for wound healing: Beyond classical strategies
    Saeed El-Ashram, Lamia M. El-Samad, Amal A. Basha, Abeer El Wakil
    Pharmacological Research.2021; 170: 105749.     CrossRef
  • Antimicrobial peptides as promising drugs for treatment of primary viral pneumonia
    M. Yu. Shchelkanov, A. V. Cybulsky, V. G. Dedkov, I. V. Galkina, V. V. Maleev
    Russian Journal of Infection and Immunity.2021; 11(5): 837.     CrossRef
  • Repurposing of Sitagliptin- Melittin Optimized Nanoformula against SARS-CoV-2; Antiviral Screening and Molecular Docking Studies
    Mohammed Al-Rabia, Nabil Alhakamy, Osama Ahmed, Khalid Eljaaly, Ahmed Alaofi, Ahmed Mostafa, Hani Asfour, Ahmed Aldarmahi, Khaled Darwish, Tarek Ibrahim, Usama Fahmy
    Pharmaceutics.2021; 13(3): 307.     CrossRef
  • Fighting against the second wave of COVID-19: Can honeybee products help protect against the pandemic?
    Yahya Al Naggar, John P. Giesy, Mohamed M. Abdel-Daim, Mohammad Javed Ansari, Saad N. Al-Kahtani, Galal Yahya
    Saudi Journal of Biological Sciences.2021; 28(3): 1519.     CrossRef
  • Bee products as a source of promising therapeutic and chemoprophylaxis strategies against COVID‐19 (SARS‐CoV‐2)
    William G. Lima, Júlio C. M. Brito, Waleska S. da Cruz Nizer
    Phytotherapy Research.2021; 35(2): 743.     CrossRef
  • Stable Loading and Delivery of Melittin with Lipid-Coated Polymeric Nanoparticles for Effective Tumor Therapy with Negligible Systemic Toxicity
    Ran Ye, Yuan Zheng, Yang Chen, Xiaohui Wei, Sanyuan Shi, Yuetan Chen, Wanxin Zhu, Anqi Wang, Liuxin Yang, Yuhong Xu, Jinliang Peng
    ACS Applied Materials & Interfaces.2021; 13(47): 55902.     CrossRef
  • Cytotoxic Effect of Bee (A. mellifera) Venom on Cancer Cell Lines
    Sima Khalilifard Borojeni, Hossein Zolfagharian, Mahdi Babaie, Iraj Javadi
    Journal of Pharmacopuncture.2020; 23(4): 212.     CrossRef
  • Bee Venom in Wound Healing
    Anna Kurek-Górecka, Katarzyna Komosinska-Vassev, Anna Rzepecka-Stojko, Paweł Olczyk
    Molecules.2020; 26(1): 148.     CrossRef
  • Potent virucidal activity of honeybee “Apis mellifera” venom against Hepatitis C Virus
    Moustafa Sarhan, Alaa M.H. El-Bitar, Hak Hotta
    Toxicon.2020; 188: 55.     CrossRef
  • Bee Venom—A Potential Complementary Medicine Candidate for SARS-CoV-2 Infections
    Keneth Iceland Kasozi, Gniewko Niedbała, Mohammed Alqarni, Gerald Zirintunda, Fred Ssempijja, Simon Peter Musinguzi, Ibe Michael Usman, Kevin Matama, Helal F. Hetta, Ngala Elvis Mbiydzenyuy, Gaber El-Saber Batiha, Amany Magdy Beshbishy, Susan Christina We
    Frontiers in Public Health.2020;[Epub]     CrossRef
  • Membrane Active Peptides Remove Surface Adsorbed Protein Corona From Extracellular Vesicles of Red Blood Cells
    Priyanka Singh, Imola Cs. Szigyártó, Maria Ricci, Ferenc Zsila, Tünde Juhász, Judith Mihály, Szilvia Bősze, Éva Bulyáki, József Kardos, Diána Kitka, Zoltán Varga, Tamás Beke-Somfai
    Frontiers in Chemistry.2020;[Epub]     CrossRef
  • Antimicrobial Properties of Apis mellifera’s Bee Venom
    Hesham El-Seedi, Aida Abd El-Wahed, Nermeen Yosri, Syed Ghulam Musharraf, Lei Chen, Moustafa Moustafa, Xiaobo Zou, Saleh Al-Mousawi, Zhiming Guo, Alfi Khatib, Shaden Khalifa
    Toxins.2020; 12(7): 451.     CrossRef
  • Linear and dendrimeric antiviral peptides: design, chemical synthesis and activity against human respiratory syncytial virus
    Ksenia V. Kozhikhova, Igor P. Shilovskiy, Artem A. Shatilov, Anastasiia V. Timofeeva, Evgeny A. Turetskiy, Liudmila I. Vishniakova, Aleksandr A. Nikolskii, Ekaterina D. Barvinskaya, Subramani Karthikeyan, Valeriy V. Smirnov, Dmitriy A. Kudlay, Sergey M. A
    Journal of Materials Chemistry B.2020; 8(13): 2607.     CrossRef
  • Bee Products in Dermatology and Skin Care
    Anna Kurek-Górecka, Michał Górecki, Anna Rzepecka-Stojko, Radosław Balwierz, Jerzy Stojko
    Molecules.2020; 25(3): 556.     CrossRef
  • Melittin: a venom-derived peptide with promising anti-viral properties
    Hamed Memariani, Mojtaba Memariani, Hamideh Moravvej, Mohammad Shahidi-Dadras
    European Journal of Clinical Microbiology & Infectious Diseases.2020; 39(1): 5.     CrossRef
  • Beekeepers who tolerate bee stings are not protected against SARS-CoV-2 infections
    Heidrun Männle, Jutta Hübner, Karsten Münstedt
    Toxicon.2020; 187: 279.     CrossRef
  • Antimicrobials from Venomous Animals: An Overview
    Tania Yacoub, Mohamad Rima, Marc Karam, Jean-Marc Sabatier, Ziad Fajloun
    Molecules.2020; 25(10): 2402.     CrossRef
  • Bee Venom: An Updating Review of Its Bioactive Molecules and Its Health Applications
    Maria Carpena, Bernabe Nuñez-Estevez, Anton Soria-Lopez, Jesus Simal-Gandara
    Nutrients.2020; 12(11): 3360.     CrossRef
  • Development of an antibacterial surface with a self-defensive and pH-responsive function
    Jing Zhang, Wenhe Zhu, Benkai Xin, Sue Lin, Libo Jin, Huiyan Wang
    Biomaterials Science.2019; 7(9): 3795.     CrossRef
  • Bee Venom: Overview of Main Compounds and Bioactivities for Therapeutic Interests
    Rim Wehbe, Jacinthe Frangieh, Mohamad Rima, Dany El Obeid, Jean-Marc Sabatier, Ziad Fajloun
    Molecules.2019; 24(16): 2997.     CrossRef
  • Bee Venom and Its Peptide Component Melittin Suppress Growth and Migration of Melanoma Cells via Inhibition of PI3K/AKT/mTOR and MAPK Pathways
    Haet Nim Lim, Seung Bae Baek, Hye Jin Jung
    Molecules.2019; 24(5): 929.     CrossRef
  • The Unique Protein Composition of Honey Revealed by Comprehensive Proteomic Analysis: Allergens, Venom-like Proteins, Antibacterial Properties, Royal Jelly Proteins, Serine Proteases, and Their Inhibitors
    Tomas Erban, Elena Shcherbachenko, Pavel Talacko, Karel Harant
    Journal of Natural Products.2019; 82(5): 1217.     CrossRef
  • Bee products and the treatment of blister-like lesions around the mouth, skin and genitalia caused by herpes viruses—A systematic review
    Karsten Münstedt
    Complementary Therapies in Medicine.2019; 43: 81.     CrossRef
  • Prospects For the Use of Peptides against Respiratory Syncytial Virus
    I. P. Shilovskiy, S. M. Andreev, K. V. Kozhikhova, A. A. Nikolskii, M. R. Khaitov
    Molecular Biology.2019; 53(4): 484.     CrossRef
  • Use of Selected Carbon Nanoparticles as Melittin Carriers for MCF-7 and MDA-MB-231 Human Breast Cancer Cells
    Karolina Daniluk, Marta Kutwin, Marta Grodzik, Mateusz Wierzbicki, Barbara Strojny, Jarosław Szczepaniak, Jaśmina Bałaban, Malwina Sosnowska, Andre Chwalibog, Ewa Sawosz, Sławomir Jaworski
    Materials.2019; 13(1): 90.     CrossRef
  • Potential Therapeutic Applications of Bee Venom on Skin Disease and Its Mechanisms: A Literature Review
    Haejoong Kim, Soo-Yeon Park, Gihyun Lee
    Toxins.2019; 11(7): 374.     CrossRef
  • An overview of the bioactive compounds, therapeutic properties and toxic effects of apitoxin
    Ananias Pascoal, Maria Manuela Estevinho, Altino Branco Choupina, Mário Sousa-Pimenta, Leticia M. Estevinho
    Food and Chemical Toxicology.2019; 134: 110864.     CrossRef
  • Hemin and bile pigments are the secondary structure regulators of intrinsically disordered antimicrobial peptides
    Ferenc Zsila, Tünde Juhász, Szilvia Bősze, Kata Horváti, Tamás Beke‐Somfai
    Chirality.2018; 30(2): 195.     CrossRef
  • Antimicrobial Activity of Bee Venom and Melittin against Borrelia burgdorferi
    Kayla Socarras, Priyanka Theophilus, Jason Torres, Khusali Gupta, Eva Sapi
    Antibiotics.2017; 6(4): 31.     CrossRef
  • Production of Antibodies in Chick by Bees Venom
    Mariyum Naz Gondal
    SSRN Electronic Journal .2017;[Epub]     CrossRef
Molecular characterization of mammalian-adapted Korean-type avian H9N2 virus and evaluation of its virulence in mice
Kuk Jin Park , Min-Suk Song , Eun-Ha Kim , Hyeok-il Kwon , Yun Hee Baek , Eun-hye Choi , Su-Jin Park , Se Mi Kim , Young-il Kim , Won-Suk Choi , Dae-Won Yoo , Chul-Joong Kim , Young Ki Choi
J. Microbiol. 2015;53(8):570-577.   Published online July 31, 2015
DOI: https://doi.org/10.1007/s12275-015-5329-4
  • 1,045 View
  • 0 Download
  • 16 Crossref
AbstractAbstract PDF
Avian influenza A virus (AIV) is commonly isolated from domestic poultry and wild migratory birds, and the H9N2 subtype is the most prevalent and the major cause of severe disease in poultry in Korea. In addition to the veterinary concerns regarding the H9N2 subtype, it is also considered to be the next potential human pandemic strain due to its rapid evolution and interspecies transmission. In this study, we utilize serial lung-to-lung passage of a low pathogenic avian influenza virus (LPAI) H9N2 (A/Ck/Korea/163/04, WT163) (Y439-lineage) in mice to increase pathogenicity and investigate the potential virulence marker. Mouse-adapted H9N2 virus obtained high virulence (100% mortality) in mice after 98 serial passages. Sequence results show that the mouse adaptation (ma163) possesses several mutations within seven gene segments (PB2, PA, HA, NP, NA, M, and NS) relative to the wild-type strain. The HA gene showed the most mutations (at least 11) with one resulting in the loss of an N-glycosylation site (at amino acid 166). Moreover, reverse genetic studies established that an E627K substitution in PB2 and the loss of the N-glycosylation site in the HA protein (aa166) are critical virulence markers in the mouse-adapted H9N2 virus. Thus, these results add to the increasing body of mutational analysis data defining the function of the viral polymerase and HA genes and their roles in mammalian host adaptation. To our knowledge, this is first report of the generation of a mammalian-adapted Korea H9N2 virus (Y493-lineages). Therefore, this study offers valuable insights into the molecular evolution of the LPAI Korean H9N2 in a new host and adds to the current knowledge of the molecular markers associated with increased virulence.

Citations

Citations to this article as recorded by  
  • Genetic Characterization of Highly Similar H9N2 Viruses Isolated from Swine and Poultry in China
    Tianqi Hong, Wei Zhang, Chenlu Xia, Yuzhong Zhao
    Microbial Pathogenesis.2026; : 108868.     CrossRef
  • Genetic Characterization of Kazakhstan Isolates: Avian Influenza H9N2 Viruses Demonstrate Their Potential to Infect Mammals
    Barshagul Baikara, Kobey Karamendin, Yermukhammet Kassymbekov, Klara Daulbayeva, Temirlan Sabyrzhan, Sardor Nuralibekov, Yelizaveta Khan, Nurlan Sandybayev, Sasan Fereidouni, Aidyn Kydyrmanov
    Viruses.2025; 17(5): 685.     CrossRef
  • An Influenza A virus can evolve to use human ANP32E through altering polymerase dimerization
    Carol M. Sheppard, Daniel H. Goldhill, Olivia C. Swann, Ecco Staller, Rebecca Penn, Olivia K. Platt, Ksenia Sukhova, Laury Baillon, Rebecca Frise, Thomas P. Peacock, Ervin Fodor, Wendy S. Barclay
    Nature Communications.2023;[Epub]     CrossRef
  • Current situation and control strategies of H9N2 avian influenza in South Korea
    Mingeun Sagong, Kwang-Nyeong Lee, Eun-Kyoung Lee, Hyunmi Kang, Young Ki Choi, Youn-Jeong Lee
    Journal of Veterinary Science.2023;[Epub]     CrossRef
  • Antigenic Evolution Characteristics and Immunological Evaluation of H9N2 Avian Influenza Viruses from 1994–2019 in China
    Qingzheng Liu, Lingcai Zhao, Yanna Guo, Yongzhen Zhao, Yingfei Li, Na Chen, Yuanlu Lu, Mengqi Yu, Lulu Deng, Jihui Ping
    Viruses.2022; 14(4): 726.     CrossRef
  • Molecular epidemiology and pathogenicity of H5N1 and H9N2 avian influenza viruses in clinically affected chickens on farms in Bangladesh
    Ripatun Nahar Ripa, Joshua E. Sealy, Jayna Raghwani, Tridip Das, Himel Barua, Md. Masuduzzaman, A. K. M. Saifuddin, Md. Reajul Huq, Mohammad Inkeyas Uddin, Munir Iqbal, Ian Brown, Nicola S. Lewis, Dirk Pfeiffer, Guillaume Fournie, Paritosh Kumar Biswas
    Emerging Microbes & Infections.2021; 10(1): 2223.     CrossRef
  • Mouse adaptation of the H9N2 avian influenza virus causes the downregulation of genes related to innate immune responses and ubiquitin-mediated proteolysis in mice
    Jing Guo, Xinxin Gao, Baotao Liu, Yubao Li, Wenqiang Liu, Jianbiao Lu, Cheng Liu, Rui Xue, Xuyong Li
    Medical Microbiology and Immunology.2020; 209(2): 151.     CrossRef
  • H9 Influenza Viruses: An Emerging Challenge
    Silvia Carnaccini, Daniel R. Perez
    Cold Spring Harbor Perspectives in Medicine.2020; 10(6): a038588.     CrossRef
  • Adaptive amino acid substitutions enable transmission of an H9N2 avian influenza virus in guinea pigs
    Liu Lina, Chen Saijuan, Wang Chengyu, Lu Yuefeng, Dong Shishan, Chen Ligong, Guo Kangkang, Guo Zhendong, Li Jiakai, Zhang Jianhui, Luo Qingping, Zhang Wenting, Shang Yu, Wang Honglin, Zhang Tengfei, Wen Guoyuan, Zhu Jiping, Zhang Chunmao, Jin Meilin, Gao
    Scientific Reports.2019;[Epub]     CrossRef
  • A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
    Haruhiko Kamiki, Hiromichi Matsugo, Tomoya Kobayashi, Hiroho Ishida, Akiko Takenaka-Uema, Shin Murakami, Taisuke Horimoto
    Viruses.2018; 10(11): 653.     CrossRef
  • Genetics and biological property analysis of Korea lineage of influenza A H9N2 viruses
    Min Kang, Hyung-Kwan Jang
    Veterinary Microbiology.2017; 204: 96.     CrossRef
  • The significance of avian influenza virus mouse-adaptation and its application in characterizing the efficacy of new vaccines and therapeutic agents
    Won-Suk Choi, Khristine Kaith S. Lloren, Yun Hee Baek, Min-Suk Song
    Clinical and Experimental Vaccine Research.2017; 6(2): 83.     CrossRef
  • Rapid acquisition of polymorphic virulence markers during adaptation of highly pathogenic avian influenza H5N8 virus in the mouse
    Won-Suk Choi, Yun Hee Baek, Jin Jung Kwon, Ju Hwan Jeong, Su-Jin Park, Young-il Kim, Sun-Woo Yoon, Jungwon Hwang, Myung Hee Kim, Chul-Joong Kim, Richard J. Webby, Young Ki Choi, Min-Suk Song
    Scientific Reports.2017;[Epub]     CrossRef
  • Vaccine Efficacy of Inactivated, Chimeric Hemagglutinin H9/H5N2 Avian Influenza Virus and Its Suitability for the Marker Vaccine Strategy
    Se Mi Kim, Young-Il Kim, Su-Jin Park, Eun-Ha Kim, Hyeok-il Kwon, Young-Jae Si, In-Won Lee, Min-Suk Song, Young Ki Choi, Jae U. Jung
    Journal of Virology.2017;[Epub]     CrossRef
  • Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014
    Duong Mai Thuy, Thomas P. Peacock, Vu Thi Ngoc Bich, Thomas Fabrizio, Dang Nguyen Hoang, Nguyen Dang Tho, Nguyen Thi Diep, Minh Nguyen, Le Nguyen Minh Hoa, Hau Thi Thu Trang, Marc Choisy, Ken Inui, Scott Newman, Nguyen vu Trung, Rogier van Doorn, Thanh Lo
    Infection, Genetics and Evolution.2016; 44: 530.     CrossRef
  • PB2 subunit of avian influenza virus subtype H9N2: a pandemic risk factor
    Hanna Sediri, Swantje Thiele, Folker Schwalm, Gülsah Gabriel, Hans-Dieter Klenk
    Journal of General Virology.2016; 97(1): 39.     CrossRef
Evaluation of the Efficacy of a Pre-pandemic H5N1 Vaccine (MG1109) in Mouse and Ferret Models
Min-Suk Song , Ho-Jin Moon , Hyeok-il Kwon , Philippe Noriel Q. Pascua , Jun Han Lee , Yun Hee Baek , Kyu-Jin Woo , Juhee Choi , Sangho Lee , Hyunseung Yoo , In gyeong Oh , Yeup Yoon , Jong-Bok Rho , Moon-Hee Sung , Seung-Pyo Hong , Chul-Joong Kim , Young Ki Choi
J. Microbiol. 2012;50(4):715-715.
  • 517 View
  • 0 Download
AbstractAbstract PDF
In the article by Song et al. that appears in the Journal of Microbiology 2012; 50, 478-488. Page 478, the name of 7th author, Kyu-Jin Woo, should read as Gyu-Jin Woo.
Evaluation of the Efficacy of a Pre-pandemic H5N1 Vaccine (MG1109) in Mouse and Ferret Models
Min-Suk Song , Ho-Jin Moon , Hyeok-il Kwon , Philippe Noriel Q. Pascua , Jun Han Lee , Yun Hee Baek , Kyu-Jin Woo , Juhee Choi , Sangho Lee , Hyunseung Yoo , In gyeong Oh , Yeup Yoon , Jong-Bok Rho , Moon-Hee Sung , Seung-Pyo Hong , Chul-Joong Kim , Young Ki Choi
J. Microbiol. 2012;50(3):487-488.
  • 596 View
  • 3 Download
AbstractAbstract PDF
The threat of a highly pathogenic avian influenza (HPAI) H5N1 virus causing the next pandemic remains a major concern. In this study, we evaluated the immunogenicity and efficacy of an inactivated whole-virus H5N1 pre-pandemic vaccine (MG1109) formulated by Green Cross Co., Ltd containing the hemagglutinin (HA) and neuraminidase (NA) genes of the clade 1 A/Vietnam/1194/04 virus in the backbone of A/Puerto Rico/8/34 (RgVietNam/04xPR8/34). Administration of the MG1109 vaccine (2-doses) in mice and ferrets elicited high HI and SN titers in a dose-dependent manner against the homologous (RgVietNam/04xPR8/34) and various heterologous H5N1 strains, (RgKor/W149/06xPR8/34, RgCambodia/04xPR8/34, RgGuangxi/05xPR8/34), including a heterosubtypic H5N2 (A/Aquatic bird/orea/W81/05) virus. However, efficient cross-reactivity was not observed against heterosubtypic H9N2 (A/Ck/Korea/H0802/08) and H1N1 (PR/8/34) viruses. Mice immunized with 1.9 μg HA/dose of MG1109 were completely protected from lethal challenge with heterologous wild-type HPAI H5N1 A/EM/Korea/W149/06 (clade 2.2) and mouse-adapted H5N2 viruses. Furthermore, ferrets administered at least 3.8 μg HA/dose efficiently suppressed virus growth in the upper respiratory tract and lungs. Vaccinated mice and ferrets also demonstrated attenuation of clinical disease signs and limited virus spread to other organs. Thus, this vaccine provided immunogenic responses in mouse and ferret models even against challenge with heterologous HPAI H5N1 and H5N2 viruses. Since the specific strain of HPAI H5N1 virus that would potentially cause the next outbreak is unknown, pre-pandemic vaccine preparation that could provide crossprotection against various H5 strains could be a useful approach in the selection of promising candidate vaccines in the future.
Production and Prophylactic Efficacy Study of Human Papillomavirus-like Particle Expressing HPV16 L1 Capsid Protein
Jie-Yun Park , Hyun-Mi Pyo , Sun-Woo Yoon , Sun-Young Baek , Sue-Nie Park , Chul-Joong Kim , Haryoung Poo
J. Microbiol. 2002;40(4):313-318.
  • 549 View
  • 1 Download
AbstractAbstract PDF
To perform the prophylactic study of a vaccine derived from human papillomavirus (HPV) using Balb/c mice, we produced virus like particles consisting of HPV capsid protein L1 which has been reported to induce significant humoral and cellular immunity using various animal model systems. In order to produce HPV16 VLPs, the cDNA of L1 capsid protein in HPV type 16, obtained by polymerase chain reaction, was inserted into yeast expression vector, YEG[alpha]-HIR525 under the control of GAL10 promoter. The transformation of YEG[alpha]-HPV16 L1 was performed into the yeast Saccharomyces cerevisiae Y2805 by the lithium acetate method and the yeast clone expressing the highest level of L1 capsid protein of human papillomavirus type 16 was selected by Western blot analysis using anti-HPV16 L1 antibody. The purification of HPV16 VLP has been performed by the ultracentrifugation and gel-filtration methods. To validate the vaccine efficacy of the purified HPV16 VLPs and investigate the properties of HPV16 VLPs to induce humoral immunity, ELISA assay was performed. A significantly increased production of anti-HPV16 VLP antibodies was observed in sera from immunized mice. The neutralization activity of antibodies in the sera from the vaccinated mice was demonstrated by a rapid and simple assay to detect hemagglutihation inhibition activity

Journal of Microbiology : Journal of Microbiology
TOP