Journal of Microbiology

Chuangfu Chen

2 Articles

A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection: Junbo Zhang , Shuanghong Yin , Fei Guo , Ren Meng , Chuangfu Chen , Hui Zhang , Zhiqiang Li , Qiang Fu , Huijun Shi , Shengwei Hu , Wei Ni , Tiansen Li , Ke Zhang; J. Microbiol. 2014;52(8):681-688. Published online July 4, 2014; DOI: https://doi.org/10.1007/s12275-014-3689-9

1,083 View
1 Download
17 Crossref

Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Δery) was constructed to overcome these drawbacks. The growth of the Δery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Δery induced an anti-Brucellaspecific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-γ) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Δery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.

Citations

Citations to this article as recorded by

The Brucella abortus A19ΔfeuPΔfeuQ double-mutant is highly attenuated and confers protection in BALB/c mice
Zhiqiang Li, Shuli Wang, Huijun Zhang, Ruirui Li, Yanyan Cui, Jinliang Zhang, Junfang Hao, Qifeng Li
Vaccine.2026; 76: 128325. CrossRef
A Meta-Analysis of the Protective Efficacy of Brucella abortus S2308 Gene-Deletion Mutant Vaccines Compared to Conventional Vaccines in Mice
Jing Yuan, Maoyuan Gu, Rongrong Ni, Xufeng Liu, Qimin Dong, Mengdi Dong, Shuqi Dong, Zeyao Wang, Tianyue Zhang, Yuting Zhang, Zhujie Fu, Junyuan Li, Yanbing Zhang, Huan Zhang, Zhongchen Ma, Junbo Zhang, Jihai Yi, Yueli Wang
Microorganisms.2026; 14(7): 1419. CrossRef
Disruption of Erythritol Catabolism via the Deletion of Fructose-Bisphosphate Aldolase (Fba) and Transaldolase (Tal) as a Strategy to Improve the Brucella Rev1 Vaccine
Aitor Elizalde-Bielsa, Leticia Lázaro-Antón, María Jesús de Miguel, Pilar M. Muñoz, Raquel Conde-Álvarez, Amaia Zúñiga-Ripa
International Journal of Molecular Sciences.2024; 25(20): 11230. CrossRef
Establishment of picodroplet-based co-culture system to improve erythritol production in Yarrowia lipolytica
Shuang Li, Yuan Zhang, Lu Li, Yaomeng Yuan, Haoxuan Sun, Xin-Hui Xing, Xiaoyan Wang, Chong Zhang
Biochemical Engineering Journal.2023; 198: 109036. CrossRef
Characterization of Brucella abortus Mutant A19mut2, a Potential DIVA Vaccine Candidate with a Modification on Lipopolysaccharide
Hosny Ahmed Abdelgawad, Zhengmin Lian, Yi Yin, Tian Fang, Mingxing Tian, Shengqing Yu
Vaccines.2023; 11(7): 1273. CrossRef
Evaluation of Brucellosis Vaccines: A Comprehensive Review
Mohsen Heidary, Shirin Dashtbin, Roya Ghanavati, Marzie Mahdizade Ari, Narjess Bostanghadiri, Atieh Darbandi, Tahereh Navidifar, Malihe Talebi
Frontiers in Veterinary Science.2022;[Epub] CrossRef
Advances and prospects of transcription‐factor‐based biosensors in high‐throughput screening for cell factories construction
Changfan Li, Chang Wang, Jiang Zhu, Feng Xue, Xiaoman Sun, Yang Gu
Food Bioengineering.2022; 1(2): 135. CrossRef
Development of Brucella melitensis Rev.1 ΔOmp19 mutants with DIVA feature and comparison of their efficacy against three commercial vaccines in a mouse model
Ali Uslu, Osman Erganis
Molecular Immunology.2021; 133: 44. CrossRef
Insights into irr and rirA gene regulation on the virulence of Brucella melitensis M5-90
Huan Zhang, Benben Wang, Wenxing Wu, Xiaoyu Deng, Zhiran Shao, Jihai Yi, Zhen Wang, Ningning Yang, Yong Wang, Yuanzhi Wang, Chuangfu Chen
Canadian Journal of Microbiology.2020; 66(5): 351. CrossRef
Combining genetically-encoded biosensors with high throughput strain screening to maximize erythritol production in Yarrowia lipolytica
Xueliang Qiu, Peng Xu, Xinrui Zhao, Guocheng Du, Juan Zhang, Jianghua Li
Metabolic Engineering.2020; 60: 66. CrossRef
Research progress on the role of immune cells in Brucella infection
Jin Zhang
Infection International.2018; 7(1): 23. CrossRef
Development and evaluation of in murine model, of an improved live-vaccine candidate against brucellosis from to Brucella melitensis vjbR deletion mutant
Zhiqiang Li, Shuli Wang, Hui Zhang, Li Xi, Jinliang Zhang, Xiaogen Zhang, Qingfeng Zhou, Jihai Yi, Min Li, Weihua Zhang, Junbo Zhang
Microbial Pathogenesis.2018; 124: 250. CrossRef
Expression and regulation of the ery operon of Brucella melitensis in human trophoblast cells
Hui Zhang, Xiaoxia Dou, Zhiqiang Li, Yu Zhang, Jing Zhang, Fei Guo, Yuanzhi Wang, Zhen Wang, Tiansen Li, Xinli Gu, Chuangfu Chen
Experimental and Therapeutic Medicine.2016; 12(4): 2723. CrossRef
Protection efficacy of the Brucella abortus ghost vaccine candidate lysed by the N-terminal 24-amino acid fragment (GI24) of the 36-amino acid peptide PMAP-36 (porcine myeloid antimicrobial peptide 36) in murine models
Ae Jeong KWON, Ja Young MOON, Won Kyong KIM, Suk KIM, Jin HUR
Journal of Veterinary Medical Science.2016; 78(10): 1541. CrossRef
Meta-Analysis and Advancement of Brucellosis Vaccinology
Tatiane F. Carvalho, João Paulo A. Haddad, Tatiane A. Paixão, Renato L. Santos, Roy Martin Roop
PLOS ONE.2016; 11(11): e0166582. CrossRef
The Brucella melitensis M5-90 phosphoglucomutase (PGM) mutant is attenuated and confers protection against wild-type challenge in BALB/c mice
Yu Zhang, Tiansen Li, Jing Zhang, Zhiqiang Li, Yan Zhang, Zhen Wang, Hanping Feng, Yuanzhi Wang, Chuangfu Chen, Hui Zhang
World Journal of Microbiology and Biotechnology.2016;[Epub] CrossRef
A Brucella melitensis M5-90 wboA deletion strain is attenuated and enhances vaccine efficacy
Zhi-Qiang Li, Jing-Xue Shi, Wen-Dong Fu, Yu Zhang, Jing Zhang, Zhen Wang, Tian-Sen Li, Chuang-Fu Chen, Fei Guo, Hui Zhang
Molecular Immunology.2015; 66(2): 276. CrossRef

Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells: Qiang Fu , Huijun Shi , Yan Ren , Fei Guo , Wei Ni , Jun Qiao , Pengyan Wang , Hui Zhang , Chuangfu Chen; J. Microbiol. 2014;52(7):619-625. Published online June 28, 2014; DOI: https://doi.org/10.1007/s12275-014-3479-4

1,043 View
1 Download
29 Crossref

Abstract PDF

Bovine viral diarrhea virus (BVDV) is an enveloped, positive-sense, single-stranded RNA virus that belongs to the genus Pestivirus (Flaviviridae). The signaling pathways and levels of signaling molecules are altered in Madin-Darby Bovine Kidney (MDBK) cells infected with BVDV. Autophagy is a conservative biological degradation pathway that mainly eliminates and degrades damaged or superfluous organelles and macromolecular complexes for intracellular recycling in eukaryotic cells. Autophagy can also be induced as an effective response to maintain cellular homeostasis in response to different stresses, such as nutrient or growth factor deprivation, hypoxia, reactive oxygen species exposure and pathogen infection. However, the effects of BVDV infection on autophagy inMDBK cells remain unclear. Therefore, we performed an analysis of autophagic activity after BVDV NADL infection using real-time PCR, electron microscopy, laser confocal microscopy, and Western blotting analysis. The results demonstrated that BVDV NADL infection increased autophagic activity and significantly elevated the expression levels of the autophagy-related genes Beclin1 and ATG14 inMDBK cells. However, the knockdown of Beclin1 and ATG14 by RNA interference (RNAi) did not affect BVDV NADL infection-related autophagic activity. These findings provided a novel perspective to elaborate the effects of viral infection on the host cells.

Citations

Citations to this article as recorded by

Bovine viral diarrhea virus suppresses type I IFN production by inducing MAVS degradation via autophagy mediated by the ROS-endoplasmic reticulum stress axis
Jing Wang, Jiangfei Zhou, Yixin Wang, Wenlu Fan, Xinyue Xia, Jiarui Chen, Haiyue Zhu, Qianyao Wang, Xiao Li, Yimei Liu, Jiayi Xiang, Han Yu, Moxuan Mao, Renjie Xu, Jiacun Liu, Shuo Jia, Yuan Li, Yigang Xu, Christiane E. Wobus
Journal of Virology.2026;[Epub] CrossRef
Pestiviruses utilize pyrimidine metabolism to regulate mitophagy for viral replication
Bingqian Zhao, Jing Chen, Yan Cheng, Yuhang Li, Linhan Zhong, Jinxia Chen, Xiaoqing Bi, Jishan Bai, Qi Dai, Yinbo Ye, Linke Zou, Li Wang, Bin Zhou
Autophagy.2026; 22(7): 1657. CrossRef
BVDV antagonizes the antiviral activity of MDA5 by promoting its degradation via CCDC50-dependent selective autophagy
Zhen Wang, Shizhe Liu, Liang Xu, Jingyu Wang, Shaotang Ye, Siqi Cai, Kun Jia, Shoujun Li
Veterinary Microbiology.2025; 307: 110601. CrossRef
TRIM5α/Cyclophilin A-Modified MDBK Cells for Lentiviral-Based Gene Editing
Lijing Wo, Shuhui Qi, Yongqi Guo, Chao Sun, Xin Yin
Viruses.2025; 17(7): 876. CrossRef
Advances in Serum-Free Suspension Culture Technology for Animal Cells and Their Applications
Wenna Ji, Ziyi Chen, Jinyu Zhou, Xinyu Yue, Zilin Qiao, Jiamin Wang
Vaccines.2025; 13(11): 1109. CrossRef
BVDV non-structural protein 4B promoted autophagy by regulating p62/LC3-II molecule
Beiyan Wang, Kaiyue Liu, Shuyu Wei, Min Wang, Jiacheng Zhou, Shuangshuang Wu, Yazun Dong, Yixuan Ma, Hongyan Liu, Liquan Yu, Jinzhu Ma, Baifen Song
Veterinary Immunology and Immunopathology.2025; 286: 110973. CrossRef
Non-cytopathic bovine viral diarrhea virus (BVDV) inhibits innate immune responses via induction of mitophagy
Zhijun Li, Ying Zhang, Bao Zhao, Qinghong Xue, Chunjiang Wang, Siyu Wan, Jingyu Wang, Xiwen Chen, Xuefeng Qi
Veterinary Research.2024;[Epub] CrossRef
Immune evasion strategies of bovine viral diarrhea virus
Feng Pang, Qinqin Long, Min Wei
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef
bta-miR-2904 inhibits bovine viral diarrhea virus replication by targeting viral-infection-induced autophagy via ATG13
Ningning Yang, Nana Hu, Jiangwei Zhang, Jihai Yi, Zhen Wang, Yong Wang, Peng Wu, Chuangfu Chen
Archives of Virology.2023;[Epub] CrossRef
In Vivo and In Vitro Antiviral Activity of Phlorizin Against Bovine Viral Diarrhea Virus
Zecai Zhang, Jiang Huang, Zhicheng Zhao, Xueying Yuan, Chuang Li, Siyu Liu, Yueqi Cui, Yu Liu, Yulong Zhou, Zhanbo Zhu
Journal of Agricultural and Food Chemistry.2022; 70(47): 14841. CrossRef
The Multi-Faceted Role of Autophagy During Animal Virus Infection
Hui Jiang, Xianjin Kan, Chan Ding, Yingjie Sun
Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
DDIT3 Targets Innate Immunity via the DDIT3-OTUD1-MAVS Pathway To Promote Bovine Viral Diarrhea Virus Replication
Song Wang, Peili Hou, Wei Pan, Wenqi He, Daniel Chang He, Hongmei Wang, Hongbin He, Rozanne M. Sandri-Goldin
Journal of Virology.2021;[Epub] CrossRef
Fate of the germ cells in mammalian ovary: A review
Pramod K. Yadav, Anumegha Gupta, Alka Sharma, Anil Kumar Yadav, Meenakshi Tiwari, Ashutosh N. Pandey, Shilpa Prasad, Tulsidas G. Shrivastav, Shail K. Chaube
Journal of Reproductive Health and Medicine.2020; 3: 3. CrossRef
Stress proteins: the biological functions in virus infection, present and challenges for target-based antiviral drug development
Qianya Wan, Dan Song, Huangcan Li, Ming-liang He
Signal Transduction and Targeted Therapy.2020;[Epub] CrossRef
Glucocorticoid-induced autophagy and apoptosis in bone
Tiantian Wang, Xiaonan Liu, Chengqi He
Apoptosis.2020; 25(3-4): 157. CrossRef
Effects of Starvation on Antioxidant-Related Signaling Molecules, Oxidative Stress, and Autophagy in Juvenile Chinese Perch Skeletal Muscle
Ping Wu, Aimin Wang, Jia Cheng, Lin Chen, Yaxiong Pan, Honghui Li, Qi Zhang, Jiaqi Zhang, Wuying Chu, Jianshe Zhang
Marine Biotechnology.2020; 22(1): 81. CrossRef
Autophagy: A Promising Target for Age-related Osteoporosis
Tiantian Wang, Hongchen He, Shaxin Liu, Chengsen Jia, Ziyan Fan, Can Zhong, Jiadan Yu, Honghong Liu, Chengqi He
Current Drug Targets.2019; 20(3): 354. CrossRef
A Comprehensive Review of Autophagy and Its Various Roles in Infectious, Non-Infectious, and Lifestyle Diseases: Current Knowledge and Prospects for Disease Prevention, Novel Drug Design, and Therapy
Rekha Khandia, Maryam Dadar, Ashok Munjal, Kuldeep Dhama, Kumaragurubaran Karthik, Ruchi Tiwari, Mohd. Iqbal Yatoo, Hafiz M.N. Iqbal, Karam Pal Singh, Sunil K. Joshi, Wanpen Chaicumpa
Cells.2019; 8(7): 674. CrossRef
Bovine viral diarrhea virus non-structural protein NS4B induces autophagosomes in bovine kidney cells
Yuto Suda, Shin Murakami, Taisuke Horimoto
Archives of Virology.2019; 164(1): 255. CrossRef
Comprehensive analysis of circRNAs expression profiles in different periods of MDBK cells infected with bovine viral diarrhea virus
Cunyuan Li, Xiaoyue Li, Xiaoxu Hou, Wei Ni, Mengdan Zhang, Huixiang Li, Yueren Xu, Wureli Hazi, Qiman Ma, Yunfeng Zhang, Dawei Wang, Shengwei Hu
Research in Veterinary Science.2019; 125: 52. CrossRef
Germ cell depletion from mammalian ovary: possible involvement of apoptosis and autophagy
Pramod K. Yadav, Meenakshi Tiwari, Anumegha Gupta, Alka Sharma, Shilpa Prasad, Ashutosh N. Pandey, Shail K. Chaube
Journal of Biomedical Science.2018;[Epub] CrossRef
Autophagy as a target for glucocorticoid-induced osteoporosis therapy
Gengyang Shen, Hui Ren, Qi Shang, Ting Qiu, Xiang Yu, Zhida Zhang, Jinjing Huang, Wenhua Zhao, Yuzhuo Zhang, De Liang, Xiaobing Jiang
Cellular and Molecular Life Sciences.2018; 75(15): 2683. CrossRef
Analyses of long non-coding RNAs and mRNA profiling through RNA sequencing of MDBK cells at different stages of bovine viral diarrhea virus infection
Qiman Ma, Liangyuan Li, Yan Tang, Qiang Fu, Sheng Liu, Shengwei Hu, Jun Qiao, Chuangfu Chen, Wei Ni
Research in Veterinary Science.2017; 115: 508. CrossRef
Both cytopathic and non-cytopathic bovine viral diarrhea virus (BVDV) induced autophagy at a similar rate
Mrigendra K.S. Rajput, Karim Abdelsalam, Mahmoud F. Darweesh, Lyle J Braun, Jason Kerkvliet, Adam D. Hoppe, Christopher C.L. Chase
Veterinary Immunology and Immunopathology.2017; 193-194: 1. CrossRef
Roles of Autophagy in Ischemic Heart Diseases and the Modulatory Effects of Chinese Herbal Medicine
Dawei Wang, Weiqing Yu, Yuntao Liu, Guofu Zhong, Zhen Zhao, Xia Yan, Qing Liu
The American Journal of Chinese Medicine.2017; 45(07): 1401. CrossRef
Crosstalk between Autophagy and Apoptosis: Potential and Emerging Therapeutic Targets for Cardiac Diseases
Meng Li, Ping Gao, Junping Zhang
International Journal of Molecular Sciences.2016; 17(3): 332. CrossRef
Roles of p53 and ASF1A in the Reprogramming of Sheep Kidney Cells to Pluripotent Cells
Huijun Shi, Qiang Fu, Guozhong Li, Yan Ren, Shengwei Hu, Wei Ni, Fei Guo, Mengting Shi, Luping Meng, Hui Zhang, Jun Qiao, Zhiru Guo, Chuangfu Chen
Cellular Reprogramming.2015; 17(6): 441. CrossRef
Recent insights into the role of autophagy in the pathogenesis of rheumatoid arthritis
Yujie Dai, Shaoxian Hu
Rheumatology.2015; : kev337. CrossRef
Structures and Functions of Pestivirus Glycoproteins: Not Simply Surface Matters
Fun-In Wang, Ming-Chung Deng, Yu-Liang Huang, Chia-Yi Chang
Viruses.2015; 7(7): 3506. CrossRef

Author index