Journal of Microbiology

Chuan-Xi Zhang

3 Articles

Comparative Analysis of the Genomes of Bombyx mandarina and Bombyx mori Nucleopolyhedroviruses: Yi-Peng Xu , Zheng-Pei Ye , Chang-Ying Niu , Yan-Yuan Bao , Wen-Bing Wang , Wei-De Shen , Chuan-Xi Zhang; J. Microbiol. 2010;48(1):102-110. Published online March 11, 2010; DOI: https://doi.org/10.1007/s12275-009-0197-4

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The Bombyx mandarina nucleopolyhedrovirus (BomaNPV) S1 strain can infect the silkworm, Bombyx mori, but is significantly less virulent than B. mori nucleopolyhedrovirus (BmNPV) T3 strain. The complete nucleotide sequence of the S1 strain of BomaNPV was determined and compared with the BmNPV T3 strain. The circular, double stranded DNA genome of the S1 strain was 126,770 nucleotides long (GenBank accession no. FJ882854), with a G+C content of 40.23%. The genome contained 133 potential ORFs. Most of the putative proteins were more than 96% identical to homologs in the BmNPV T3 strain, except for bro-a, lef-12, bro-c, and bro-d. Compared with the BmNPV T3 strain, however, this genome did not encode the bro-b and bro-e genes. In addition, hr1 lacked two repeat units, while hr2L, hr2R, hr3, hr4L, hr4R, and hr5 were similar to the corresponding hrs in the T3 strain. The sequence strongly suggested that BomaNPV and BmNPV are variants with each other, and supported the idea that baculovirus strain heterogeneity may often be caused by variation in the hrs and bro genes.

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Distribution and genetic diversity of Bombyx mori nucleopolyhedrovirus in mass-reared silkworms in Thailand
Jörg T. Wennmann, Sergei Senger, Birgit Ruoff, Johannes A. Jehle, Siripuk Suraporn
Journal of Invertebrate Pathology.2024; 207: 108221. CrossRef
Genomic Sequence Analysis of Bombyx mori Nucleopolyhedrovirus Isolated from Yunnan Sericulture Region, China
Yonghong Zhang, Feng Zhu, Yulan Shao, Zhenguo Su, Jing Ni, Xingrong Bai
Indian Journal of Microbiology.2021; 61(3): 383. CrossRef
Bombyx mori nucleopolyhedrovirus ptp and egt genes are dispensable for triggering enhanced locomotory activity and climbing behavior in Bombyx mandarina larvae
Ryuhei Kokusho, Susumu Katsuma
Journal of Invertebrate Pathology.2021; 183: 107604. CrossRef
Whole-genome sequencing and comparative transcriptome analysis of Bombyx mori nucleopolyhedrovirus La strain
Shota Fujimoto, Munetaka Kawamoto, Keisuke Shoji, Yutaka Suzuki, Susumu Katsuma, Masashi Iwanaga
Virus Genes.2020; 56(2): 249. CrossRef
Gene Expression Pattern and Regulatory Network of α-Toxin Treatment in Bombyx mori
Tieshan Feng, Ping Lin, Jiao Gong, Dong Cheng, Xi Yang, Quan Zhang, Tingcai Cheng
International Journal of Genomics.2019; 2019: 1. CrossRef
Baculovirus Kimura two-parameter species demarcation criterion is confirmed by the distances of 38 core gene nucleotide sequences
Jörg T. Wennmann, Jens Keilwagen, Johannes A. Jehle
Journal of General Virology.2018; 99(9): 1307. CrossRef
Bombyx mori nucleopolyhedrovirus actin rearrangement-inducing factor 1 enhances systemic infection in B. mori larvae
Susumu Katsuma, Toru Shimada, Ryuhei Kokusho, Sumio Sugano, Yutaka Suzuki, Yasue Koyano, Munetaka Kawamoto
Journal of General Virology.2015; 96(7): 1938. CrossRef
Complete genome sequence of the first non-Asian isolate of Bombyx mori nucleopolyhedrovirus
Daniel Mendes Pereira Ardisson-Araújo, Fernando Lucas Melo, Miguel de Souza Andrade, Rose Meire Costa Brancalhão, Sônia Nair Báo, Bergmann Morais Ribeiro
Virus Genes.2014; 49(3): 477. CrossRef
Identification of a Conserved Non-Protein-Coding Genomic Element that Plays an Essential Role in Alphabaculovirus Pathogenesis
Irina Kikhno, Carmen San Martin
PLoS ONE.2014; 9(4): e95322. CrossRef
Analysis of the genomic sequence of Philosamia cynthia nucleopolyhedrin virus and comparison with Antheraea pernyinucleopolyhedrin virus
Heying Qian, Yuehua Zhang, Yejun Wu, Pingiang Sun, Shanying Zhu, Xijie Guo, Kun Gao, Anying Xu, Wenbing Wang
BMC Genomics.2013;[Epub] CrossRef
Genomic diversity of Bombyx mori nucleopolyhedrovirus strains
Yi-Peng Xu, Ruo-Lin Cheng, Yu Xi, Chuan-Xi Zhang
Genomics.2013; 102(1): 63. CrossRef
Genome Sequence of a Bombyx mori Nucleopolyhedrovirus Strain with Cubic Occlusion Bodies
Ruo-Lin Cheng, Yi-Peng Xu, Chuan-Xi Zhang
Journal of Virology.2012; 86(18): 10245. CrossRef
Identification of a new Bombyx mori nucleopolyhedrovirus and analysis of its bro gene family
Jing-Bo Zhou, Xing-Qi Li, Wanchai De-Eknamkul, Siripuk Suraporn, Jia-Ping Xu
Virus Genes.2012; 44(3): 539. CrossRef
A baculovirus isolated from wild silkworm encompasses the host ranges of Bombyx mori nucleopolyhedrosis virus and Autographa californica multiple nucleopolyhedrovirus in cultured cells
Yi-Peng Xu, Lin-Zhu Gu, Yi-Han Lou, Ruo-Lin Cheng, Hai-Jun Xu, Wen-Bing Wang, Chuan-Xi Zhang
Journal of General Virology .2012; 93(11): 2480. CrossRef
Genome of a Bombyx mori Nucleopolyhedrovirus Strain Isolated from India
Hai-Wei Fan, Xue-Chao Zhang, Yi-Peng Xu, Xiao-Wen Cheng, Chuan-Xi Zhang
Journal of Virology.2012; 86(21): 11941. CrossRef
Complete Sequence, Analysis and Organization of the Orgyia leucostigma Nucleopolyhedrovirus Genome
David K. Thumbi, Robert J. M. Eveleigh, Christopher J. Lucarotti, Renée Lapointe, Robert I. Graham, Lillian Pavlik, Hilary A. M. Lauzon, Basil M. Arif
Viruses.2011; 3(11): 2301. CrossRef
Comparative analysis of budded virus infectivity of Bombyx mandarina and B. mori nucleopolyhedroviruses
Ryuhei Kokusho, Chuan-Xi Zhang, Toru Shimada, Susumu Katsuma
Virus Genes.2011; 43(2): 313. CrossRef
Characterization of a late gene, ORF75 from Bombyx mori nucleopolyhedrovirus
Jun-Qing Ge, Guo-Hui Gao, Yi-Peng Xu, Chuan-Xi Zhang
Molecular Biology Reports.2011; 38(3): 2141. CrossRef
Baculovirus: Molecular Insights on Their Diversity and Conservation
Solange Ana Belen Miele, Matías Javier Garavaglia, Mariano Nicolás Belaich, Pablo Daniel Ghiringhelli
International Journal of Evolutionary Biology.2011; 2011: 1. CrossRef
Identification of a novel functional nuclear localization signal in the protein encoded by open reading frame 47 of Bombyx mori nucleopolyhedrovirus
Zhong-Jian Guo, Dian-Xuan Wang, Qin Yao, Ke-Ping Chen, Chuan-Xi Zhang
Archives of Virology.2010; 155(12): 1943. CrossRef

Characterization of a Baculovirus Newly Isolated from the Tea Slug Moth, Iragoidae fasciata: Li-Rong Yang , Xiao Qiang , Bao-Qin Zhang , Mei-Jun Tang , Chuan-Xi Zhang; J. Microbiol. 2009;47(2):208-213. Published online May 2, 2009; DOI: https://doi.org/10.1007/s12275-008-0253-5

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Abstract PDF

The tea slug moth Iragoidae fasciata (Lepidoptera, Eucleidae) is one of the main insect pests that attack tea bushes. A new nucleopolyhedrovirus (NPV) called Iragoidae fasciata NPV (IrfaNPV) was recently isolated from diseased larvae. An 11,626 bp fragment of the viral genomic DNA containing the polyhedrin gene and other 12 genes was cloned and sequenced. Gene comparison and phylogenetic analysis showed that IrfaNPV is a member of the Group I NPVs. However, the genomic organization of IrfaNPV is highly distinct. In addition, electron microscopy analysis showed that IrfaNPV is a single nucleocapsid NPV (SNPV). An inoculation assay showed that IrfaNPV is semi-permissive in the Trichoplusia ni cell line Tn-5B1-4. Bioassays on lethal concentration (LC50) and lethal time (LT50) were conducted to test the susceptibility of I. fasciata larvae to the virus.

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Granulovirus GP37 Facilitated ODVs Cross Insect Peritrophic Membranes and Fuse with Epithelia
Xiangyang Liu, Wei Fang, Rui Fan, Linna Zhang, Chengfeng Lei, Jingjing Zhang, Wenkai Nian, Tao Dou, Shiheng An, Lin Zhou, Xiulian Sun
Toxins.2019; 11(3): 145. CrossRef
Microbial management of arthropod pests of tea: current state and prospects
Somnath Roy, Narayanannair Muraleedharan
Applied Microbiology and Biotechnology.2014; 98(12): 5375. CrossRef
Molecular Characterization of a Nucleopolyhedrovirus Newly Isolated from Ophiusa disjungens in China
Tong Lin, Li Liu, Runlei Chang, Guojun Lang, Wen Xu
Indian Journal of Virology.2012; 23(3): 379. CrossRef
Synergistic effects of Cydia pomonella granulovirus GP37 on the infectivity of nucleopolyhedroviruses and the lethality of Bacillus thuringiensis
Xiangyang Liu, Xiaohe Ma, Chengfeng Lei, Yuzhou Xiao, Zhongxin Zhang, Xiulian Sun
Archives of Virology.2011; 156(10): 1707. CrossRef

Morphological, Phylogenetic and Biological Characteristics of Ectropis obliqua Single-Nucleocapsid Nucleopolyhedrovirus: Xiu-cui Ma , Hai-Jun Xu , Mei-Jun Tang , Qiang Xiao , Jian Hong , Chuan-Xi Zhang; J. Microbiol. 2006;44(1):77-82.; DOI: https://doi.org/2333 [pii]

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Abstract PDF: The tea looper caterpillar, Ectropis obliqua, is one of the major pests of tea bushes. E. obliqua single-nucleocapsid nucleopolyhedrovirus (EcobSNPV) has been used as a commercial pesticide for biocontrol of this insect. However only limited genetic analysis for this important virus has been done up to now. EcobSNPV was characterized in this study. Electron microscopy analysis of the occlusion body showed polyhedra of 0.7 to 1.7 μm in diameter containing a single nucleocapsid per envelope of the virion. A 15.5 kb genomic fragment containing EcoRI-L, EcoRI-N and HindIII-F fragments, was sequenced. Analysis of the sequence revealed that the fragment contained eleven potential open reading frames (ORFs): lef-1, egt, 38.7k, rr1, polyhedrin, orf1629, pk-1, hoar and homologues to Spodoptera exigua multicapsid NPV (SeMNPV) ORFs 15, 28, and 29. Gene arrangement and phylogeny analysis suggest that EcobSNPV is closely related to the previously described Group II NPV. Bioassays on lethal concentration (LC50 and LC90) and lethal time (LT50 and LT90) were conducted to test the susceptibility of E. obliqua larvae to the virus.

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