- Proteomic analysis reveals the temperature-dependent presence of extracytoplasmic peptidases in the biofilm exoproteome of Listeria monocytogenes EGD-e
-
Yue-Jia Lee , Chinling Wang
-
J. Microbiol. 2020;58(9):761-771. Published online July 27, 2020
-
DOI: https://doi.org/10.1007/s12275-020-9522-8
-
-
981
View
-
2
Download
-
6
Web of Science
-
6
Crossref
-
 Abstract
 PDF
-
The foodborne pathogen Listeria monocytogenes resists environmental
stresses by forming biofilms. Because this pathogen
transmits between the environment and the host, it must
adapt to temperature as an environmental stress. In this study,
we aimed to identify which proteins were present depending
on the temperature in the biofilms of L. monocytogenes
EGD-e. Proteins in the supernatants of biofilms formed at
25°C and 37°C were compared using two-dimensional gel
electrophoresis and liquid chromatography with tandem mass
spectrometry. The larger number of extracytoplasmic proteins
associated with cell wall/membrane/envelop biogenesis
was identified from the supernatant of biofilms formed at
25°C (7) than those at 37°C (0). Among the 16 extracytoplasmic
proteins detected only at 25°C, three were peptidases,
namely Spl, Cwh, and Lmo0186. Moreover, mRNA expression
of the three peptidases was higher at 25°C than at 37°C.
Interestingly, this adaptation of gene expression to temperature
was present in sessile cells but not in dispersed cells.
After inhibiting the activity of extracytoplasmic peptidases
with a protease inhibitor, we noted that the levels of biofilm
biomass increased with higher concentrations of the protease
inhibitor only when L. monocytogenes grew biofilms
at 25°C and not at 37°C. Overall, our data suggest an effect of
temperature on the presence of peptidases in L. monocytogenes
biofilms. Additionally, increasing the levels of extracytoplasmic
peptidases in biofilms is likely a unique feature for
sessile L. monocytogenes that causes a naturally occurring
breakdown of biofilms and facilitates the pathogen exiting
biofilms and disseminating into the environment.
-
Citations
Citations to this article as recorded by  - Biofilms in clinical infection: pathophysiology, diagnosis, and the evolving therapeutic landscape
Aglaia Domouchtsidou, Petros Ioannou, Alexandra Lianou, Konstantina A. Tsante, Deny Tsakri, Elli Bonova, Stella Baliou, Sotirios P. Fortis, Anastasios E. Chaldoupis, George Samonis, Christos Koutserimpas, Rozeta Sokou, Dimitrios V. Papadopoulos, Andreas G
Journal of Clinical Microbiology.2026;[Epub] CrossRef - Impact of temperature fluctuation on biofilm formation and removal of different Listeria monocytogenes strains in ice cream processing environments
Zhao Li, Jiangtian Wang, Yifan Gao, Hongzhi Zhang, Yangtai Liu, Xuejuan Xia, Weiwei Li, Lin Yan, Qingli Dong
Food Research International.2025; 221: 117416. CrossRef - Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling
Federica D’Onofrio, Maria Schirone, Antonello Paparella, Ivanka Krasteva, Manuela Tittarelli, Francesco Pomilio, Luigi Iannetti, Nicola D’Alterio, Mirella Luciani
Foods.2023; 12(11): 2166. CrossRef - How to study biofilms: technological advancements in clinical biofilm research
Leanne Cleaver, James A. Garnett
Frontiers in Cellular and Infection Microbiology.2023;[Epub] CrossRef - Studying the metabolic factors that may impact the growth of co-cultured Listeria monocytogenes strains at low temperature
Maria A. Gkerekou, Eleftheria H. Kaparakou, Petros A. Tarantilis, Panagiotis N. Skandamis
Food Research International.2023; 171: 113056. CrossRef - Exoproteome Perspective on the Bile Stress Response of Lactobacillus johnsonii
Bernadette B. Bagon, Valerie Diane V. Valeriano, Ju Kyoung Oh, Edward Alain B. Pajarillo, Ji Yoon Lee, Dae-Kyung Kang
Proteomes.2021; 9(1): 10. CrossRef
|
MSK
