Journal of Microbiology

Chi-Young Yun

2 Articles

Analysis of Cytoplasmic Membrane Proteome of Streptococcus pneumoniae by Shotgun Proteomic Approach: Chi-Won Choi , Sung-Ho Yun , Sang-Oh Kwon , Sun-Hee Leem , Jong-Soon Choi , Chi-Young Yun , Seung Il Kim; J. Microbiol. 2010;48(6):872-876. Published online January 9, 2011; DOI: https://doi.org/10.1007/s12275-010-0220-9

709 View
0 Download
9 Crossref

In this study, cytoplasmic membrane proteins of S. pneumoniae strain R6 (ATCC BBA-255) were effectively separated from cell wall or extracellular proteins by sodium carbonate precipitation (SCP) and ultracentrifugation. Forty seven proteins were analyzed as cytoplasmic membrane proteins from the 260 proteins identified by the shotgun proteomic method using SDS-PAGE/LC/MS-MS. ABC transporters for metabolites such as metals, oligopeptides, phosphate, sugar, and amino acids, and membrane proteins involved in phosphotransferse systems, were identified as the predominant and abundant, cytoplasmic membrane proteins that would be essential for nutrient uptake, antibiotic resistance and virulence mechanisms. Our result supports that gel-based shotgun proteomics combined with sodium carbonate precipitation and ultracentrifugation is an effective method for analysis of cytoplasmic membrane proteins of S. pneumoniae.

Citations

Citations to this article as recorded by

The potential linkage between antibiotic resistance genes and microbial functions across soil–plant systems
Enzong Xiao, Weimin Sun, Jinmei Deng, Li Shao, Zengping Ning, Tangfu Xiao
Plant and Soil.2023; 493(1-2): 589. CrossRef
Efficient profiling of detergent-assisted membrane proteome in cyanobacteria
Jong-Soon Choi, Yun Hwan Park, Jeong Hyun Oh, Sooyong Kim, Joseph Kwon, Yoon-E Choi
Journal of Applied Phycology.2020; 32(2): 1177. CrossRef
A partial proteome reference map of the wine lactic acid bacterium Oenococcus oeni ATCC BAA-1163
María de la Luz Mohedano, Pasquale Russo, Vivian de los Ríos, Vittorio Capozzi, Pilar Fernández de Palencia, Giuseppe Spano, Paloma López
Open Biology.2014; 4(2): 130154. CrossRef
Influence of Impaired Lipoprotein Biogenesis on Surface and Exoproteome of Streptococcus pneumoniae
Thomas Pribyl, Martin Moche, Annette Dreisbach, Jetta J.E. Bijlsma, Malek Saleh, Mohammed R. Abdullah, Michael Hecker, Jan Maarten van Dijl, Dörte Becher, Sven Hammerschmidt
Journal of Proteome Research.2014; 13(2): 650. CrossRef
Identification of Potential New Protein Vaccine Candidates through Pan-Surfomic Analysis of Pneumococcal Clinical Isolates from Adults
Alfonso Olaya-Abril, Irene Jiménez-Munguía, Lidia Gómez-Gascón, Ignacio Obando, Manuel J. Rodríguez-Ortega, Paulo Lee Ho
PLoS ONE.2013; 8(7): e70365. CrossRef
Characterization of Streptococcus pneumoniae N-acetylglucosamine-6-phosphate deacetylase as a novel diagnostic marker
Chi-Won Choi, Hee-Young An, Yong Ju Lee, Yeol Gyun Lee, Sung Ho Yun, Edmond Changkyun Park, Yeonhee Hong, Gun-Hwa Kim, Jae-Eun Park, Sun Jong Baek, Hyun Sik Kim, Seung Il Kim
Journal of Microbiology.2013; 51(5): 659. CrossRef
Reevaluation of the Harboe Assay as a Standardized Method of Assessment for the Hemolytic Performance of Ventricular Assist Devices
Chris H.H. Chan, Andrew Hilton, Graham Foster, Karl Hawkins
Artificial Organs.2012; 36(8): 724. CrossRef
Application of subproteomics in the characterization of Gram-positive bacteria
Xiao-Yan Yang, Jie Lu, Xuesong Sun, Qing-Yu He
Journal of Proteomics.2012; 75(10): 2803. CrossRef
Analysis of Streptococcus pneumoniae secreted antigens by immuno-proteomic approach
Chi-Won Choi, Yeol Gyun Lee, Sang-Oh Kwon, Hye-Yeon Kim, Je Chul Lee, Young-Ho Chung, Chi-Young Yun, Seung Il Kim
Diagnostic Microbiology and Infectious Disease.2012; 72(4): 318. CrossRef

Characterization of Protocatechuate 4,5-Dioxygenase Induced from p-Hydroxybenzoate-Cultured Pseudomonas sp. K82: Sung-Ho Yun , Chi-Young Yun , Seung Il Kim; J. Microbiol. 2004;42(2):152-155.; DOI: https://doi.org/2029 [pii]

536 View
0 Download

Abstract PDF: Pseudomonas sp. K82 has been reported to be an aniline-assimilating soil bacterium. However, this strain can use not only aniline as a sole carbon and energy source, but can also utilize benzoate, phydroxybenzoate, and aniline analogues. The strain accomplishes this metabolic diversity by using different aerobic pathways. Pseudomonas sp. K82, when cultured in p-hydroxybenzoate, showed extradiol cleavage activity of protocatechuate. In accordance with those findings, our study attempted the purification of protocatechuate 4,5-dioxygenase (PCD 4,5). However the purified PCD 4,5 was found to be very unstable during purification. After Q-sepharose chromatography was performed, the crude enzyme activity was augmented by a factor of approximately 4.7. From the Q-sepharose fraction which exhibited PCD 4,5 activity, two subunits of PCD4,5 ([alpha]subunit and [beta] subunit) were identified using the N-terminal amino acid sequences of 15 amino acid residues. These subunits were found to have more than 90% sequence homology with PmdA and PmdB of Comamonas testosteroni. The molecular weight of the native enzyme was estimated to be approximately 54 kDa, suggesting that PCD4,5 exists as a heterodimer ([alpha]_1[beta]_1). PCD 4,5 exhibits stringent substrate specificity for protocatechuate and its optimal activity occurs at pH 9 and 15^oC. PCR amplification of these two subunits of PCD4,5 revealed that the [alpha] subunit and [beta] subunit occurred in tandem. Our results suggest that Pseudomonas sp. K82 induced PCD 4,5 for the purpose of p-hydroxybenzoate degradation.

Author index