- Adaptation and Cross-Adaptation of Listeria monocytogenes and Salmonella enterica to Poultry Decontaminants
-
Alicia Alonso-Hernando , Rosa Capita , Miguel Prieto , Carlos Alonso-Calleja
-
J. Microbiol. 2009;47(2):142-146. Published online May 2, 2009
-
DOI: https://doi.org/10.1007/s12275-008-0237-5
-
-
801
View
-
0
Download
-
24
Crossref
-
 Abstract
 PDF
-
Information on the potential for acquired reduced susceptibility of bacteria to poultry decontaminants occurring is lacking. Minimal Inhibitory Concentrations (MICs) were established for assessing the initial susceptibility and the adaptative and cross-adaptative responses of four bacterial strains (Listeria monocytogenes serovar 1/2a, L. monocytogenes serovar 4b, Salmonella enterica serotype Typhimurium, and S. enterica serotype Enteritidis) to four poultry decontaminants (trisodium phosphate, acidified sodium chlorite -ASC-, citric acid, and peroxyacetic acid). The initial susceptibility was observed to differ among species (all decontaminants) and between Salmonella strains (ASC). These inter- and intra-specific variations highlight (1) the need for strict monitoring of decontaminant concentrations to inactivate all target pathogens of concern, and (2) the importance of selecting adequate test strains in decontamination studies. MICs of ASC (0.17±0.02 to 0.21±0.02 mg/ml) were higher than the U.S. authorized concentration when applied as a pre-chiller or chiller solution (0.05 to 0.15 mg/ml). Progressively increasing decontaminant concentrations resulted in reduced susceptibility of strains. The highest increase in MIC was 1.88 to 2.71-fold (ASC). All decontaminants were shown to cause cross-adaptation of strains between both related and unrelated compounds, the highest increase in MIC being 1.82-fold (ASC). Our results suggest that the in-use concentrations of ASC could, in certain conditions, be ineffective against Listeria and Salmonella strains. The adaptative and cross-adaptative responses of strains tested to poultry decontaminants are of minor concern. However, the observations being presented here are based on in vitro studies, and further research into practical applications are needed in order to confirm these findings.
-
Citations
Citations to this article as recorded by  - Deciphering poultry microbial ecosystems by classical and modern tools
Natalia Merino, Laura Espina, Daniel Berdejo, Rafael Pagán, Diego García-Gonzalo
Food Bioscience.2026; 77: 108356. CrossRef - Exposure-driven adaptation of Listeria monocytogenes to disinfectants yields distinct resistance phenotypes depending on the strain
Aminta Vega-Sánchez, Guillem Expósito-Flores, Paula Cruz-Vera, Pedro Rolón-Verdún, Guillem Gervilla-Cantero, Carolina Ripolles-Avila
International Journal of Food Microbiology.2026; 451: 111665. CrossRef - Exposure to Low Doses of Biocides Increases Resistance to Other Biocides and to Antibiotics in Strains of Listeria monocytogenes
Cristina Rodríguez-Melcón, Rosa Capita, Carlos Alonso-Calleja
Biology.2025; 14(5): 495. CrossRef - Pseudomonadaceae increased the tolerance of Listeria monocytogenes to sanitizers in multi-species biofilms
Olena Voloshchuk, M. Laura Rolon, Katelyn V. Bartlett, Marysabel Mendez Acevedo, Luke F. LaBorde, Jasna Kovac
Food Microbiology.2025; 128: 104687. CrossRef - Overuse of food-grade disinfectants threatens a global spread of antimicrobial-resistant bacteria
Ricardo A. Wu, Jinsong Feng, Min Yue, Donghong Liu, Tian Ding
Critical Reviews in Food Science and Nutrition.2024; 64(19): 6870. CrossRef - Estimation by flow cytometry of percentages of survival of Listeria monocytogenes cells treated with tetracycline, with or without prior exposure to several biocides
Cristina Rodríguez-Melcón, Víctor Serrano-Galán, Rosa Capita, Carlos Alonso-Calleja
Food Microbiology.2023; 112: 104210. CrossRef - Effect of low doses of biocides on the susceptibility of Listeria monocytogenes and Salmonella enterica to various antibiotics of clinical importance
Cristina Rodríguez-Melcón, Carlos Alonso-Calleja, Rosa Capita
Food Control.2023; 149: 109602. CrossRef - Exploring Peracetic Acid and Acidic pH Tolerance of Antibiotic-Resistant Non-Typhoidal Salmonella and Enterococcus faecium from Diverse Epidemiological and Genetic Backgrounds
Andreia Rebelo, Bárbara Duarte, Ana R. Freitas, Luísa Peixe, Patrícia Antunes, Carla Novais
Microorganisms.2023; 11(9): 2330. CrossRef - Unraveling the Role of Metals and Organic Acids in Bacterial Antimicrobial Resistance in the Food Chain
Andreia Rebelo, Agostinho Almeida, Luísa Peixe, Patrícia Antunes, Carla Novais
Antibiotics.2023; 12(9): 1474. CrossRef - In Vitro Investigation of the Antibacterial Activity of Nine Commercial Water Disinfectants, Acidifiers, and Glyceride Blends against the Most Important Poultry Zoonotic Bacteria
Tilemachos Mantzios, Vasilios Tsiouris, Konstantinos Kiskinis, Vangelis Economou, Evanthia Petridou, Anestis Tsitsos, Apostolos Patsias, Ioanna Apostolou, Georgios A. Papadopoulos, Ilias Giannenas, Paschalis Fortomaris
Pathogens.2023; 12(3): 381. CrossRef - Probing antimicrobial resistance and sanitizer tolerance themes and their implications for the food industry through the Listeria monocytogenes lens
Rebecca Bland, Stephanie R. B. Brown, Joy Waite‐Cusic, Jovana Kovacevic
Comprehensive Reviews in Food Science and Food Safety.2022; 21(2): 1777. CrossRef - Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) for Twelve Antimicrobials (Biocides and Antibiotics) in Eight Strains of Listeria monocytogenes
Cristina Rodríguez-Melcón, Carlos Alonso-Calleja, Camino García-Fernández, Javier Carballo, Rosa Capita
Biology.2021; 11(1): 46. CrossRef - Adaptive Response of Listeria monocytogenes to the Stress Factors in the Food Processing Environment
Natalia Wiktorczyk-Kapischke, Krzysztof Skowron, Katarzyna Grudlewska-Buda, Ewa Wałecka-Zacharska, Jakub Korkus, Eugenia Gospodarek-Komkowska
Frontiers in Microbiology.2021;[Epub] CrossRef - Antimicrobial resistance associated with the use of antimicrobial processing aids during poultry processing operations: cause for concern?
Mohamed Rhouma, Pablo Romero-Barrios, Marie-Lou Gaucher, Sujinder Bhachoo
Critical Reviews in Food Science and Nutrition.2021; 61(19): 3279. CrossRef - Screening of antimicrobial synergism between phenolic acids derivatives and UV-A light radiation
Erick F. de Oliveira, Xu Yang, Nikeshala Basnayake, Cuong Nguyen Huu, Luxin Wang, Rohan Tikekar, Nitin Nitin
Journal of Photochemistry and Photobiology B: Biology.2021; 214: 112081. CrossRef - Exposure of ciprofloxacin-resistant Escherichia coli broiler isolates to subinhibitory concentrations of a quaternary ammonium compound does not increase antibiotic resistance gene transfer
H. Maertens, K. De Reu, E. Meyer, S. Van Weyenberg, J. Dewulf, E. Van Coillie
Poultry Science.2019; 98(7): 2972. CrossRef - Thermal inactivation of Salmonella Enteritidis on chicken skin previously exposed to acidified Sodium chlorite or tri-sodium phosphate
K. Karuppasamy, Ajit S. Yadav, Gaurav K. Saxena
Journal of Food Science and Technology.2015; 52(12): 8236. CrossRef - Scientific Opinion on the evaluation of the safety and efficacy of peroxyacetic acid solutions for reduction of pathogens on poultry carcasses and meat
EFSA Journal.2014;[Epub] CrossRef - Growth kinetic parameters of Gram-positive and Gram-negative bacteria on poultry treated with various chemical decontaminants
Alicia Alonso-Hernando, Carlos Alonso-Calleja, Rosa Capita
Food Control.2013; 33(2): 429. CrossRef - Decontamination treatments can increase the prevalence of resistance to antibiotics of Escherichia coli naturally present on poultry
Rosa Capita, Elena Álvarez-Fernández, Esther Fernández-Buelta, Jennifer Manteca, Carlos Alonso-Calleja
Food Microbiology.2013; 34(1): 112. CrossRef - Antibiotic-Resistant Bacteria: A Challenge for the Food Industry
Rosa Capita, Carlos Alonso-Calleja
Critical Reviews in Food Science and Nutrition.2013; 53(1): 11. CrossRef - Effects of exposure to poultry chemical decontaminants on the membrane fluidity of Listeria monocytogenes and Salmonella enterica strains
Alicia Alonso-Hernando, Carlos Alonso-Calleja, Rosa Capita
International Journal of Food Microbiology.2010; 137(2-3): 130. CrossRef - Comparison of antibiotic resistance patterns in Listeria monocytogenes and Salmonella enterica strains pre-exposed and exposed to poultry decontaminants
Alicia Alonso-Hernando, Rosa Capita, Miguel Prieto, Carlos Alonso-Calleja
Food Control.2009; 20(12): 1108. CrossRef - Comparative analysis of acid resistance in Listeria monocytogenes and Salmonella enterica strains before and after exposure to poultry decontaminants. Role of the glutamate decarboxylase (GAD) system
Alicia Alonso-Hernando, Carlos Alonso-Calleja, Rosa Capita
Food Microbiology.2009; 26(8): 905. CrossRef
- Assessment of Baird-Parker Agar as Screening Test for Determination of Staphylococcus aureus in Poultry Meat
-
Rosa Capita , Carlos Alonso-Calleja , Benito Moreno , Maria del Camino Garcia-Fernandez
-
J. Microbiol. 2001;39(4):321-325.
-
-
-
Abstract
PDF
-
Baird-Parker agar with egg yolk/tellurite emulsion (BPA) is widely accepted as a medium for the enumeration of Staphylococcus aureus in foods. However, it is not completely selective and colonies of other genera or species could be similar to those of Staphylococcus aureus. Moreover, the strains of Staphylococcus aureus that are lecithinase negative could go unnoticed. Both facts could affect the counts. The aim of this study was to determine whether the enumeration of the colonies with the typical morphology of Staphylococcus aureus on BPA is sufficient to quantify this species in poultry meat. Forty chicken carcasses were tested for Staphylococcus aureus by surface plating using BPA. Results indicate that the predictive value of the morphology of the colonies on BPA is 85.71% and 68.42% for typical and atypical colonies of Staphylococcus aureus, respectively. However, Staphylococcus aureus counts (after identification) and counts of typical colonies did not show any significant differences (P>0.05) and are significantly (P<0.001) correlated (r = 0.996). These results suggest that, for screening purposes, enumeration of Staphylococcus aureus from poultry meat does not require any identification of strains, resulting in a saving of time and money.
- Evaluation of the EF-18 Agar-Hydrophobic Grid Membrane Filter (HGMF) Method to Isolate Salmonella from Poultry Products
-
Rosa Capita , Maite Alvarez-Astorga , Carlos Alonso-Calleja , Maria del Camino , Garcia-Fernandez , Benito Moreno
-
J. Microbiol. 2001;39(3):202-205.
-
-
-
Abstract
PDF
-
The EF-18 agar/hydrophobic grid membrane filter (EF18/HGMF) method was evaluated for the isolation of Salmonella in naturally contaminated chicken carcasses, chicken parts (legs, wings and giblets) and processed chicken products (sausages and hamburgers). Percentages of false positive results for Salmonella (colonies with a similar morphology to those of Salmonella) were 78.75, 81.67 and 80% for carcasses, chicken parts and processed chicken products, respectively. The bacterial isolates that caused false positive reactions using this method were identified as Proteus mirabilis (70.85%), Citrobacter freundii (15.25%), Klebsiella ozaenae (5.83%), Hafnia alvei (4.48%), Escherichia coli (2.69%) and Enterobacter aerogenes (0.90%). The data obtained in this study suggest that the EF-18/HGMF method is not sufficiently selective or specific for isolating Salmonella from meat and chicken products.
- Phage Typing of Staphylococcus aureus Isolates from Poultry Meat in Spain
-
Rosa Capita , Maite Alvarez-Astorga , Carlos Alonso-Calleja , Benito Moreno , Maria del Camino Garcia-Fernandez
-
J. Microbiol. 2001;39(3):219-225.
-
-
-
Abstract
PDF
-
Phage typing is currently used for typing of Staphylococcus aureus strains beyond the species level in epidemiological studies. A total of 168 Staphylococcus aureus isolates from chicken meat and chicken by-products were phage-typed using the international bacteriophage set for typing Staphylococcus aureus of human origin. One hundred and forty-eight (88.09%) strains were phage-typeable (at least one phage produced 20 or more plaques of lysis). Lysis by phages of group III was the most frequent with 99 (58.93%) sensitive strains. This fact coincides with results of other authors. Twenty-nine different phage patterns were observed and three (95, 75/84 and 6/1030/W57) were most common. One hundred and thirty-two (89.19% of typeable strains) showed these or indistinguishable (only one phage reaction difference) patterns. Twenty-six out of seventy chicken samples (37.14%) harboured more than one phage type of Staphylococcus aureus. This fact emphasizes the convenience of subtyping several Staphylococcus aureus isolates from the same sample in epidemiological studies. 80% of sausages and hamburgers contained the same Staphylococcus aureus phage types, which were not found in any of the other food types. This fact suggests a cross contamination during the processing of these foods. Phages 6, 75, 84, 1030 and W57 showed the greatest activity. None of the Staphylococcus aureus strains were sensitive to phages 47, 81 and 94.
|
MSK
