Journal of Microbiology

Byeong Chul Jeong

5 Articles

Nomenclature of ISCR1 Elements Capable of Mobilizing Antibiotic Resistance Genes Present in Complex Class 1 Integrons: Seung Ghyu Sohn , Jae Jin Lee , Jae Seok Song , Jung Hun Lee , Ha Ik Sun , Kwang Seung Park , Il Kwon Bae , Jung-Hyun Lee , Byeong Chul Jeong , Sang Hee Lee; J. Microbiol. 2009;47(4):514-516. Published online September 9, 2009; DOI: https://doi.org/10.1007/s12275-009-0054-5

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The dissemination of many antibiotic resistance genes has arisen among members of the family Enterobacteriaceae. The dissemination mechanism of these antibiotic resistance genes is closely linked with insertion sequence common region 1 (ISCR1). Thus, caution must be taken in clinical settings to prevent further dissemination of these antibiotic resistance genes. A nomenclature system of ISCR1 variants, important for the antibiotic resistance dissemination, was proposed. The proposed system can designate all ISCR1 variants on the basis of the detection time and by considering amino-acid substitution(s) compared with ISCR1a. This nomenclature system of ISCR1 variants can be applied to 19 groups (ISCR1 to ISCR19) of the ISCR family and help some researchers to correctly designate new ISCR subgroups.

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Molecular Characteristics of Carbapenem-Resistant Gram-Negative Bacteria in Southern China
Fen Zheng, Jingjing Sun, Cancan Cheng, Yongyu Rui
Microbial Drug Resistance.2015; 21(2): 178. CrossRef
Novel ISCR1-linked resistance genes found in multidrug-resistant Gram-negative bacteria in southern China
Fengping Wang, Kuihai Wu, Jingjing Sun, Qian Wang, Qing Chen, Shouyi Yu, Yongyu Rui
International Journal of Antimicrobial Agents.2012; 40(5): 404. CrossRef
Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens
Hatch W. Stokes, Michael R. Gillings
FEMS Microbiology Reviews.2011; 35(5): 790. CrossRef

Removal of Contaminating TEM-la β-Lactamase Gene from Removal of Contaminating TEM-la β-Lactamase Gene from: Jae Seok Song , Jung Hun Lee , Jung-Hyun Lee , Byeong Chul Jeong , Won-Keun Lee , Sang Hee Lee; J. Microbiol. 2006;44(1):126-128.; DOI: https://doi.org/2326 [pii]

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Abstract PDF: This study confirms that Taq DNA polymerase could be contaminated with the blaTEM-1a gene. It also proposes two different methods that could be used to overcome DNA contamination: (i) DNase I treatment prior to PCR amplification; and (ii) the use of a highly purified Taq DNA polymerase which was devoid of detectable contamination.

Molecular Characterization of TEM-type [beta]-Lactamases Identified in Cold-Seep Sediments of Edison Seamount (South of Lihir Island, Papua New Guinea): Jae Seok Song , Jeong Ho Jeon , Jung Hun Lee , Seok Hoon Jeong , Byeong Chul Jeong , Sang-Jin Kim , Jung-Hyun Lee , Sang Hee Lee; J. Microbiol. 2005;43(2):172-178.; DOI: https://doi.org/2165 [pii]

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Abstract PDF: To determine the prevalence and genotypes of [beta]-lactamases among clones of a metagenomic library from the cold-seep sediments of Edison seamount (10,000 years old), we performed pulse-field gel electrophoresis, antibiotic susceptibility testing, pI determination, and DNA sequencing analysis. Among the 8,823 clones of the library, thirty clones produced [beta]-lactamases and had high levels of genetic diversity. Consistent with minimum inhibitory concentration patterns, we found that five (16.7%) of thirty clones produced an extended-spectrum [beta]-lactamase. 837- and 259-bp fragments specific to bla_TEM genes were amplified, as determined by banding patterns of PCR amplification with designed primers. TEM-1 was the most prevalent [beta]-lactamase and conferred resistance to ampicillin, piperacillin, and cephalothin. TEM-116 had a spectrum that was extended to ceftazidime, cefotaxime, and aztreonam. The resistance levels conferred by the pre-antibiotic era alleles of TEM-type [beta]-lactamases were essentially the same as the resistance levels conferred by the TEM-type alleles which had been isolated from clinically resistant strains of bacteria of the antibiotic era. Our first report on TEM-type [beta]-lactamases of the pre-antibiotic era indicates that TEM-type [beta]-lactamases paint a picture in which most of the diversity of the enzymes may not be the result of recent evolution, but that of ancient evolution.

Overexpression of the sprD Gene Encoding Streptomyces griseus Protease D Stimulates Actinorhodin Production in Streptomyces lividans: Si-Sun Choi , Won-Jae Chi , Jae Hag Lee , Sang-Soon Kang , Byeong Chul Jeong , Soon-Kwang Hong; J. Microbiol. 2001;39(4):305-313.

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Abstract PDF: The sprD gene encoding Streptomyces griseus protease D (SGPD), a chymotrypsin-like protease, was cloned from Streptomyces griseus IFO13350 and sequenced. Most of the amino-acid sequence deduced from the nucleotide sequence is identical to that of Streptomyces griseus IMRU3499 except that one amino acid has been deleted and Trp369 has been substituted into Cys369 in the SGPD from S. griseus IFO13350 without affecting the protease activity. The sprD gene was overexpressed in Streptomyces lividans TK24 as a heterologous host. Various media with different compositions were also used to maximize the productivity of SGPD in the heterologous host. The SGPD productivity was best when the transformant of S. lividans TK24 was cultivated in R2YE medium. The relative chymotrypsin activity of the culture broth measured with an artificial chromogenic substrate, N-succinyl-ala-ala-pro-phe-[rho]-nitroanilide, was 16 units/ml. A high level of SGPD was also produced in YEME and SAAM media but it was relatively lower than in R2YE medium, and negligible amounts of SGPD were produced in GYE, GAE and Benedict media. The growth of S. lividans reached the maximum level of cell mass at days 3 and 4 of the culture, but SGPD production started in the stationary phase of cell growth and kept increasing till the 10th day of culture in R2YE and YEME medium, but in GYE media the productivity reached maximum level at 8 days of cultivation. The introduction of the sprD gene into S. lividans TK24 triggered biosynthesis of the pigmented antibiotic, actinorhodin, which implies some protease may play a very important role in secondary-metabolite formation in Streptomyces.

Reduction of Hexavalent Chromium by Escherichia coli ATCC 33456 in Batch and Continuous Cultures: Woo Chul Bae , Tae Gu Kang , In Kyong Kang , You Jung Won , Byeong Chul Jeong; J. Microbiol. 2000;38(1):36-39.

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Abstract PDF: Toxic hexavalent chromium, Cr(VI), was reduced to a less toxic trivalent chromium form by E. coli ATCC 33456. The suitable electron donor for Cr(VI) reduction was glucose. E. coli ATCC 33456 was more resistant to metal cations than other reported Cr(VI) reducing microorganisms. Cell growth was inhibited by the presence of Cr(VI) in a liquid medium and Cr(VI) reduction accompanied cell growth. With a hydraulic retention time of 20 h, Cr(VI) reducing efficiency was 100% to 84% when Cr(VI) concentration in the influent was in the range of 10 to 40 mg L^-1. Specific rate of Cr(VI) concentration in the influent was 2.41 mg Cr(VI) g DCW^-1 h^-1 when 40 mg :^-1 of Cr(VI) influent was used. This result suggested the potential application of E. coli ATCC 33456 for the detoxification of Cr(VI) in Cr(VI) contaminated wastewater.

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