Journal of Microbiology

Bong Whan Sung

1 Article

A Recombinant Mouse GM-CSF Protein Expressed as an Inclusion Form Shows Colony Stimulating Activity: Jin-Kyoo Kim , Eun-Jung Sohn , Soo-O Lee , Choon-Taek Lee , Ah Young Lee , Hye Kyung Chung , Bong Whan Sung , Hyun Joo Youn; J. Microbiol. 2000;38(2):109-112.

Abstract PDF: Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a pleiotropic hematopoietic growth factor and an activator of mature myeloid cells, and recombinant GM-CSF is increasingly under clinical studies for the treatment of various diseases including cancer, infectious diseases and hematopoietic diseases. We constructed a recombinant mouse GM-CSF expression plasmid with pelB leader sequence and His.Tag under T7 promoter control, and showed that the construct produced a 20 kDa recombinant protein in 8M urea. We also showed that the 20 kDa recombinant protein prepared in 8M urea stimulated colony formation in vitro, indicating that the recombinant mGM-CSF can be renatured to its native form to show the colony stimulating activity.

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