- Cloning and Characterization of the Gene Cluster for Biosynthesis of Ectoine from Nesterenkonia halobia DSM 20541
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Bo Zhang , Xin Bao , Lei Wang , Su Sheng Yang
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J. Microbiol. 2008;46(3):309-318. Published online July 5, 2008
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DOI: https://doi.org/10.1007/s12275-008-0001-x
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The ectABC genes encoding the biosynthesis of ectoine were identified from Nesterenkonia halobia DSM 20541. The intergenic regions of the ectABC genes from N. halobia DSM 20541 were more loosely spaced than those that had been reported before. The amino acid sequence deduced from ectABC of the strain was highly homologous to the EctABC of Brevibacterium linens BL2 (EctA 50%, EctB 70%, and EctC 68% identities). The osmoprotection of ectABC was studied in the Escherichia coli KNabc and E. coli XL1-Blue. The results revealed that ectABC could shorten the lag phase and enhance the final OD600 of E. coli XL1-Blue in MM63 medium containing 0.68 M NaCl, and could initiate KNabc growth in 0.2 M NaCl. Ectoine was proven to be accumulated in E. coli KNabc/pGEM-Nect using HPLC-UV, and validated by LC-MSD-Trap-VL.
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Citations
Citations to this article as recorded by  - Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor
Hao Zhang, Zhong Liang, Ming Zhao, Yanqin Ma, Zhengshan Luo, Sha Li, Hong Xu
Frontiers in Bioengineering and Biotechnology.2022;[Epub] CrossRef - Identification of novel halophilic/halotolerant bacterial species producing compatible solutes
Furkan Orhan, Ertuğrul Ceyran
International Microbiology.2022; 26(2): 219. CrossRef - Development and validation of polar RP-HPLC method for screening for ectoine high-yield strains in marine bacteria with green chemistry
Jun Chen, Jianwei Chen, Sijia Wang, Guangmin Zhou, Danqing Chen, Huawei Zhang, Hong Wang
Natural Product Research.2019; 33(8): 1122. CrossRef
- Protein Expression Analysis of Halobacillus dabanensis D-8T Subjected to Salt Shock
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De Qin Feng , Bo Zhang , Wei Dong Lu , Su Sheng Yang
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J. Microbiol. 2006;44(4):369-374.
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DOI: https://doi.org/2418 [pii]
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Abstract
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To investigate the mechanism of salt tolerance of gram-positive moderately halophilic
bacteria, two-dimensional gel electrophoresis (2-D PAGE) was employed to achieve high resolution maps of proteins of Halobacillus dabanensis D-8T. Approximately 700 spots of proteins were identified from these 2-D PAGE maps. The majority of these proteins had molecular weights between 17.5 and 66 kDa, and most of them were distributed between the isoelectric points (pI) 4.0 and 5.9. Some protein spots were distributed in the more acidic region of the 2-D gel (pI <4.0). This pattern indicated that a number of proteins in the strain D-8T are acidic. To understand the adaptation mechanisms of moderately halophilic bacteria in response to sudden environmental changes, differential protein profiles of this strain were investigated by 2-D PAGE and ImagemasterTM 2D Platinum software after the cells were subjected to salt shock of 1 to 25% salinity for 5 and 50 min. Analysis showed 59 proteins with an altered level of expression as the result of the exposure to salt shock. Eighteen proteins had increased expression, 8 proteins were induced, and the expression of 33 proteins was down-regulated. Eight of the up-regulated proteins were identified using MALDI-TOF/MS and MASCOT, and were similar to proteins involved in signal transduction, proteins participating in energy metabolism pathways and proteins involved in stress.
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