Journal of Microbiology

Bo Sun

2 Articles

H2 Metabolism revealed by metagenomic analysis of subglacial sediment from East Antarctica: Zhifeng Yang , Yu Zhang , Yongxin Lv , Wenkai Yan , Xiang Xiao , Bo Sun , Hongmei Ma; J. Microbiol. 2019;57(12):1095-1104. Published online November 22, 2019; DOI: https://doi.org/10.1007/s12275-019-9366-2

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Subglacial ecosystems harbor diverse chemoautotrophic microbial communities in areas with limited organic carbon, and lithological H2 produced during glacial erosion has been considered an important energy source in these ecosystems. To verify the H2-utilizing potential there and to identify the related energy-converting metabolic mechanisms of these communities, we performed metagenomic analysis on subglacial sediment samples from East Antarctica with and without H2 supplementation. Genes coding for several [NiFe]- hydrogenases were identified in raw sediment and were enriched after H2 incubation. All genes in the dissimilatory nitrate reduction and denitrification pathways were detected in the subglacial community, and the genes coding for these pathways became enriched after H2 was supplied. Similarly, genes transcribing key enzymes in the Calvin cycle were detected in raw sediment and were also enriched. Moreover, key genes involved in H2 oxidization, nitrate reduction, oxidative phosphorylation, and the Calvin cycle were identified within one metagenome-assembled genome belonging to a Polaromonas sp. As suggested by our results, the microbial community in the subglacial environment we investigated consisted of chemoautotrophic populations supported by H2 oxidation. These results further confirm the importance of H2 in the cryosphere.

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Analysis of nifH Gene Diversity in Red Soil Amended with Manure in Jiangxi, South China: Qihui Teng , Bo Sun , Xinrui Fu , Shunpeng Li , Zhongli Cui , Hui Cao; J. Microbiol. 2009;47(2):135-141. Published online May 2, 2009; DOI: https://doi.org/10.1007/s12275-008-0184-1

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Abstract PDF

In order to understand the community structure of diazotrophs in red soil and effects of organic manure application on the structure, four nifH gene libraries were constructed: the control (CK), low manure (LM), high manure (HM), and high manure adding lime (ML). Totally 150 nifH gene clones were screened and grouped into 21 clusters by RFLP analysis. Existence of dominant patterns was observed in all libraries, which counted for over 96% of clones in library HM and about 56~72% in other three libraries. The nifH sequences of the dominant patterns in all libraries were most similar to sequences of the cyanobacteria. nifH genes showed high diversity in red soil, dispersing throughout the nifH clades (alpha-, beta-, and gamma-Proteobacteria, Firmicutes, cyanobacteria, Verrucomicrobia, and posited group). Bradyrhizobium and Burkholderia were also important diazotrophs in low fertility soil samples. Low manure treatment increased the diversity of nifH genes compared with CK and high manure treatments. Manure and lime treatment led to obvious community succession. Total N to available P ratio, total carbon, and K concentrations were the main factors affecting the diversity of diazotrophs in red soil.

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Ping Pan, Fang Zhao, Jinkui Ning, Ling Zhang, Xunzhi Ouyang, Hao Zang, Jorge Paz-Ferreiro
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Priscila A. Calderoli, Mónica M. Collavino, Filipe Behrends Kraemer, Héctor J. M. Morrás, O. Mario Aguilar
MicrobiologyOpen.2017;[Epub] CrossRef
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