Journal of Microbiology

Bo Liu

2 Articles

Functional Characterization of the Genes tauO, tauK, and tauI in the Biosynthesis of Tautomycetin: Fen Wang , Rixiang Kong , Bo Liu , Jing Zhao , Rongguo Qiu , Li Tang; J. Microbiol. 2012;50(5):770-776. Published online November 4, 2012; DOI: https://doi.org/10.1007/s12275-012-2154-x

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Tautomycetin is a specific protein phosphatase I inhibitor. In an effort to elucidate the biosynthetic mechanism of tautomycetin, we inactivated genes of the tautomycetin biosynthetic gene cluster, tauI, tauO, and tauK, which encode for putative P450 oxidase, citryl-CoA lyase, and esterase enzymes, respectively. The mutant STQ0606 (ΔtauO) did not produce any detectable amount of tautomycetin intermediates but could convert dialkylmaleic anhydride to tautomycetin, strongly indicating that TauO was involved in dialkylmaleic anhydride biosynthesis. STQ1211 (ΔtauK) accumulated dialkylmaleic anhydride, whereas the cofermentation of STQ1211 (ΔtauK) and STQ0606 (ΔtauO) restored the production of tautomycetin. Together, these results suggest that TauK was responsible for the conjugation of dialkylmaleic anhydride and the polyketide moiety in tautomycetin biosynthesis. The disruption of tauI resulted in the accumulation of 5-des-ketotautomycetin, revealing that TauI was responsible for the oxidation at C5 as the last step. Although the shunt pathways were involved in the biosynthesis of tautomycetin, the main post-polyketide synthase tailoring steps were dehydration, decarboxylation and oxidation, taking place consecutively. This study allowed us to predict the biosynthesis of tautomycetin more accurately and provided novel insights into the mechanism of the biosynthesis of tautomycetin.

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Biocatalytic role of cytochrome P450s to produce antibiotics: A review
Anup Adhikari, Sajan Shakya, Shreesti Shrestha, Dipa Aryal, Kavi Prasad Timalsina, Dipesh Dhakal, Yogan Khatri, Niranjan Parajuli
Biotechnology and Bioengineering.2023; 120(12): 3465. CrossRef
Biosynthesis, regulation, and engineering of a linear polyketide tautomycetin: a novel immunosuppressant in Streptomyces sp. CK4412
Si-Sun Choi, Hee-Ju Nah, Hye-rim Pyeon, Eung-Soo Kim
Journal of Industrial Microbiology and Biotechnology.2017; 44(4-5): 555. CrossRef
Optimized transformation of Streptomyces sp. ATCC 39366 producing leptomycin by electroporation
Yong-Qiang Fan, Hong-Jian Liu, Li Yan, Yu-Shi Luan, Hai-Meng Zhou, Jun-Mo Yang, Shang-Jun Yin, Yu-Long Wang
Journal of Microbiology.2013; 51(3): 318. CrossRef
A Novel Alkaloid from Marine-Derived Actinomycete Streptomyces xinghaiensis with Broad-Spectrum Antibacterial and Cytotoxic Activities
Wence Jiao, Fenghua Zhang, Xinqing Zhao, Jiehan Hu, Joo-Won Suh, Marie-Joelle Virolle
PLoS ONE.2013; 8(10): e75994. CrossRef

Differential Expression Profiles of Alternaria alternate Genes in Response to Carbonyl Sulfide Fumigation: Tao Liu , Li Li , Yuejin Wang , Guoping Zhan , Bo Liu; J. Microbiol. 2010;48(4):480-485. Published online August 20, 2010; DOI: https://doi.org/10.1007/s12275-010-9301-z

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Abstract PDF

Carbonyl sulfide (COS) is a new fumigant used in phytosanitary treatments. It was developed as a potential alternative to methyl bromide, which is being phased out because of its ozone-depletion properties. To understand the molecular and cellular mechanisms occurring in fungal pathogens in response to COS fumigation, we cloned 510 cDNA fragments of Alternaria alternata (Fr.) Keissler genes that are differentially expressed; these genes were cloned using suppression subtractive hybridization. Changes in the levels of transcripts of 79 fragments were confirmed by microarray analysis and qRT-PCR. Further homology search revealed that they are highly homologous to 41 genes of other fungi, which were related to general metabolism, growth and division, defense, cellular transport, and signal transduction. These results provide an overview of differential expression profiles of A. alternata genes following COS treatment and some new clues about the mechanism of COS fungitoxicity.

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Potential inhibitors of methionine aminopeptidase type II identified via structure-based pharmacophore modeling
Safana Albayati, Abdullahi Ibrahim Uba, Kemal Yelekçi
Molecular Diversity.2022; 26(2): 1005. CrossRef
Transcriptional inhibition of the Catalase gene in phosphine-induced oxidative stress in Drosophila melanogaster
Tao Liu, Li Li, Fanhua Zhang, Yuejin Wang
Pesticide Biochemistry and Physiology.2015; 124: 1. CrossRef
Analysis of a suppressive subtractive hybridization library of Alternaria alternata resistant to 2-propenyl isothiocyanate
Heriberto García-Coronado, Rosalba Troncoso-Rojas, Martín Ernesto Tiznado-Hernández, María del Carmen de la Cruz Otero, Sylvia Páz Díaz-Camacho, María Elena Báez-Flores
Electronic Journal of Biotechnology.2015; 18(4): 320. CrossRef
Sulfur Fertilization and Fungal Infections Affect the Exchange of H2S and COS from Agricultural Crops
Elke Bloem, Silvia Haneklaus, Jürgen Kesselmeier, Ewald Schnug
Journal of Agricultural and Food Chemistry.2012; 60(31): 7588. CrossRef
Sulfurous Gases As Biological Messengers and Toxins: Comparative Genetics of Their Metabolism in Model Organisms
Neal D. Mathew, David I. Schlipalius, Paul R. Ebert
Journal of Toxicology.2011; 2011: 1. CrossRef

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