- Investigation of Waterborne Parasites in Drinking Water Sources of Ankara, Turkey
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Bilal Bakir , Mehmet Tanyuksel , Fatma Saylam , Sultan Tanriverdi , R. Engin Araz , Ali Kasim Hacim , Metin Hasde
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J. Microbiol. 2003;41(2):148-151.
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 Abstract
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Waterborne parasite infections are considered a re-emerging threat. Most studies on the epidemiology of human cryptosporidiosis, giardiasis, and amebiasis have been carried out in developed countries, and there is little data on the occurrence of these infections in other areas. The objective of this study was to investigate the presence of waterborne parasites such as Cryptosporidium parvum, Giardia lam-blia, and Entamoeba histolytica in various water samples in Ankara, Turkey. A total of 85 samples were examined, 43 from the municipal water supply, 34 from wells, 6 from the Ankara River, and 2 from two untreated dams; by conventional microscopy, immunologically and by polymerase chain reaction (PCR). Oocysts of C. parvum and cysts of G. lamblia were detected by using an indirect fluorescence (antigen) assay, whereas an enzyme linked immunosorbent assay was used to detect the cysts of E. his-tolytica and E. dispar. In addition, PCR was used for E. histolytica, E. dispar, C. parvum and G. lamblia detection. G. lamblia was found in 2 of the 34 well water samples, and parasites were found in 3 of the 6 Ankara River samples. The 1^st contained E. histolytica cysts and Strongyloides stercoralis larvae, the 2^nd E. histolytica cysts, and Trichuris trichiura eggs, and the 3^rd C. parvum oocysts only. No parasite was observed in the municipal water samples and untreated dam water samples. These results extend our knowledge on waterborne parasites, such occurrence information on waterborne pathogens assists the management and treatment of municipal water.
- Determination of Enteric Bacteria at Microbiologically Risky Points by Multiplex Polymerase Chain Reaction
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Mahir Gulec , Bilal Bakir , Recai Ogur , Omer Faruk Tekbas
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J. Microbiol. 2002;40(4):327-330.
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The purpose of this research was to test multiplex polymerase chain reaction in investigating the microbiological quality of the risky surfaces in social living places of a military base where over 15 thousand people live together. In 22 samples of 99, there were no bacteria. Only four of the samples contained Shigella, and one of them contained Salmonella, but 77 of the samples contained thermotolerant coliform organisms. There was no statistically significant difference among the microbiological quality of different sites and different equipment surfaces (p>0.05).
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