- Identification and Characterization of Acetyl-CoA Carboxylase Gene Cluster in Streptomyces toxytricini
-
Atanas V. Demirev , Ji Seon Lee , Bhishma R. Sedai , Ivan G. Ivanov , Doo Hyun Nam
-
J. Microbiol. 2009;47(4):473-478. Published online September 9, 2009
-
DOI: https://doi.org/10.1007/s12275-009-0135-5
-
-
843
View
-
0
Download
-
12
Crossref
-
 Abstract
 PDF
-
The gene locus for acetyl-CoA carboxylase (ACC) involved in the primary metabolism was identified from the genomic library of Streptomyces toxytricini which produces a lipase inhibitor lipstatin. The 7.4 kb cloned gene was comprised of 5 ORFs including accD1, accA1, hmgL, fadST1, and stsF. In order to confirm the biochemical characteristics of AccA1, the gene was overexpressed in Escherichia coli cells, and the recombinant protein was purified through Ni2+ affinity chromatography. Because most of the expressed AccA1 was biotinylated by host E. coli BirA in the presence of D-biotin, the non-biotinylated apo-AccA1 was purified after gene induction without D-biotin, followed by exclusion of holo-AccA1 using streptavidin beads. The separated apo-AccA1 was post-translationally biotinylated by S. toxytricini biotin apo-protein ligase (BPL) in a time- and enzyme-dependent manner. This result supports that this gene cluster of S. toxytricini encodes the functional ACC enzyme subunits to be biotinylated.
-
Citations
Citations to this article as recorded by  - Experimental investigation of multi-strain bacterial concrete: Self-healing efficiency, strength, and sorptivity under varied curing conditions
Ran Bir Singh, Ashish Kumar, Divya Sharma, Surya Kant Sahdeo
Journal of Building Engineering.2026; 120: 115552. CrossRef - Origin of the 3-methylglutaryl moiety in caprazamycin biosynthesis
Daniel Bär, Benjamin Konetschny, Andreas Kulik, Houchao Xu, Davide Paccagnella, Patrick Beller, Nadine Ziemert, Jeroen S. Dickschat, Bertolt Gust
Microbial Cell Factories.2022;[Epub] CrossRef - Biotechnological and industrial applications of Streptomyces metabolites
Khushboo, Punit Kumar, Kashyap K. Dubey, Zeba Usmani, Minaxi Sharma, Vijai Kumar Gupta
Biofuels, Bioproducts and Biorefining.2022; 16(1): 244. CrossRef - Development and Optimization of a High-Throughput Screening Assay for Rapid Evaluation of Lipstatin Production by Streptomyces Strains
Michal Híreš, Nora Rapavá, Martin Šimkovič, Ľudovít Varečka, Dušan Berkeš, Svetlana Kryštofová
Current Microbiology.2018; 75(5): 580. CrossRef - De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor
Laura Marín, Ignacio Gutiérrez-del-Río, Rodrigo Entrialgo-Cadierno, Claudio J. Villar, Felipe Lombó, Marie-Joelle Virolle
PLOS ONE.2018; 13(11): e0207278. CrossRef - Regulation and structure of the heteromeric acetyl-CoA carboxylase
Matthew J. Salie, Jay J. Thelen
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids.2016; 1861(9): 1207. CrossRef - Current trends and future prospects of lipstatin: a lipase inhibitor and pro-drug for obesity
Punit Kumar, Kashyap Kumar Dubey
RSC Advances.2015; 5(106): 86954. CrossRef - Enhanced production of lipstatin from Streptomyces toxytricini by optimizing fermentation conditions and medium
Tingheng Zhu, Lingfei Wang, Weixia Wang, Zhongce Hu, Meilan Yu, Kun Wang, Zhifeng Cui
The Journal of General and Applied Microbiology.2014; 60(3): 106. CrossRef -
Transcriptional Response to Vancomycin in a Highly Vancomycin-Resistant
Streptomyces coelicolor
Mutant
Fernando Santos-Beneit, Lorena T Fernández-Martínez, Antonio Rodríguez-García, Seomara Martín-Martín, María Ordóñez-Robles, Paula Yagüe, Angel Manteca, Juan F Martín
Future Microbiology.2014; 9(5): 603. CrossRef - Equilibrium binding and kinetic characterization of putative tetracycline repressor family transcription regulator Fad35R from Mycobacterium tuberculosis
Sushma Anand, Vijay Singh, Appu Kumar Singh, Monica Mittal, Manish Datt, Bala Subramani, Sangaralingam Kumaran
The FEBS Journal.2012; 279(17): 3214. CrossRef - Biochemical characteristization of propionyl-coenzyme a carboxylase complex of Streptomyces toxytricini
Atanas V. Demirev, Anamika Khanal, Nguyen Phan Kieu Hanh, Kyung Tae Nam, Doo Hyun Nam
The Journal of Microbiology.2011; 49(3): 407. CrossRef - The role of acyl-coenzyme A carboxylase complex in lipstatin biosynthesis of Streptomyces toxytricini
Atanas V. Demirev, Anamika Khanal, Bhishma R. Sedai, Si Kyu Lim, Min Kyun Na, Doo Hyun Nam
Applied Microbiology and Biotechnology.2010; 87(3): 1129. CrossRef
|
MSK
