Journal of Microbiology

Ashraf M. Ahmed

1 Article

A Novel Retron of Vibrio parahaemolyticus Is Closely Related to Retron-Vc95 of Vibrio cholerae: Toshi Shimamoto , Ashraf M. Ahmed , Tadashi Shimamoto; J. Microbiol. 2013;51(3):323-328. Published online June 28, 2013; DOI: https://doi.org/10.1007/s12275-013-2715-7

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Some bacteria produce a satellite RNA-DNA complex termed msDNA, multicopy single-stranded DNA. In this report, msDNA from Vibrio parahaemolyticus, a cause of acute gastroenteritis, was identified and named msDNA-Vp96. The retron element containing the ret gene, encoding the reverse transcriptase (RT) that is responsible for msDNA production, was cloned and characterized. Comparison of msDNAVp96 and msDNA-Vc95, from Vibrio cholerae, showed a high level of sequence similarity. We exchanged the two ret genes to examine whether msDNA was produced by the RT from different sources. We found that RT-Vp96 of V. parahaemolyticus was able to synthesize msDNA-Vc95 of V. cholerae and vice versa. To the best of our knowledge, this is the first report that RT from different bacterial species can synthesize msDNA.

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Retrons and their applications in genome engineering
Anna J Simon, Andrew D Ellington, Ilya J Finkelstein
Nucleic Acids Research.2019; 47(21): 11007. CrossRef
An Unexplored Diversity of Reverse Transcriptases in Bacteria
Steven Zimmerly, Li Wu, Alan Lambowitz, Nancy Craig
Microbiology Spectrum.2015;[Epub] CrossRef

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