Journal of Microbiology

Arbakariya Bin Ariff

1 Article

Improved Production of Live Cells of Lactobacillus rhamnosus by Continuous Cultivation using Glucose-yeast Extract Medium: Liew Siew Ling , Rosfarizan Mohamad , Raha Abdul Rahim , Ho Yin Wan , Arbakariya Bin Ariff; J. Microbiol. 2006;44(4):439-446.; DOI: https://doi.org/2408 [pii]

Abstract PDF: In this study, the growth kinetics of Lactobacillus rhamnosus and lactic acid production in continuous culture were assessed at a range of dilution rates (0.05 h?1 to 0.40 h?1) using a 2 L stirred tank fermenter with a working volume of 600 ml. Unstructured models, predicated on the Monod and Luedeking-Piret equations, were employed to simulate the growth of the bacterium, glucose consumption, and lactic acid production at different dilution rates in continuous cultures. The maximum specific growth rate of L. rhamnosus, ┢max, was estimated at 0.40 h?1, and the Monod cell growth saturation constant, Ks, at approximately 0.25 g/L. Maximum cell viability (1.3 ≠ 1010 CFU/ml) was achieved in the dilution rate range of D = 0.28 h?1 to 0.35 h?1. Both maximum viable cell yield and productivity were achieved at D = 0.35 h?1. The continuous cultivation of L. rhamnosus at D = 0.35 h?1 resulted in substantial improvements in cell productivity, of 267% (viable cell count) that achieved via batch cultivation.

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