- The protein and neutral lipid composition of lipid droplets isolated from the fission yeast, Schizosaccharomyces pombe
-
Alex Meyers , Karuna Chourey , Taylor M. Weiskittel , Susan Pfiffner , John R. Dunlap , Robert L. Hettich , Paul Dalhaimer
-
J. Microbiol. 2017;55(2):112-122. Published online January 26, 2017
-
DOI: https://doi.org/10.1007/s12275-017-6205-1
-
-
969
View
-
2
Download
-
18
Crossref
-
 Abstract
 PDF
-
Lipid droplets consist of a core of neutral lipids surrounded
by a phospholipid monolayer with bound proteins. Much of
the information on lipid droplet function comes from proteomic
and lipodomic studies that identify the components
of droplets isolated from organisms throughout the phylogenetic
tree. Here, we add to that important inventory by reporting
lipid droplet factors from the fission yeast, Schizosaccharomyces
pombe. Unique to this study was the fact that cells were
cultured in three different environments: 1) late log growth
phase in glucose-based media, 2) stationary phase in glucosebased
media, and 3) late log growth phase in media containing
oleic acid. We confirmed colocalization of major factors
with lipid droplets using live-cell fluorescent microscopy. We
also analyzed droplets from each of the three conditions for
sterol ester (SE) and triacylglycerol (TAG) content, along
with their respective fatty acid compositions. We identified
a previously undiscovered lipid droplet protein, Vip1p, which
affects droplet size distribution. The results provide further
insight into the workings of these ubiquitous organelles.
-
Citations
Citations to this article as recorded by  - HOPS and Vps13 Have Antagonistic Roles in Regulating Lipid Droplet Morphology
Jie Wang, Saidaiguli Abulimiti, Jingping Chen, Xinping Zheng, Wanjing Chen, Yang Lei, Xingya Xu
Cells.2026; 15(19): 1724. CrossRef - Lipid dysregulation in polyglutamine diseases
Luhan Ni, Vaishnavi Veeranki, Byron Huang, Janghoo Lim
Molecules and Cells.2026; : 100406. CrossRef - Cbf11 and Mga2 function together to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast
Anna Marešová, Michaela Grulyová, Miluše Hradilová, Viacheslav Zemlianski, Jarmila Princová, Martin Převorovský, Cathy Savage-Dunn
PLOS Genetics.2024; 20(12): e1011509. CrossRef - Mild Heat Stress Alters the Physical State and Structure of Membranes in Triacylglycerol-Deficient Fission Yeast, Schizosaccharomyces pombe
Péter Gudmann, Imre Gombos, Mária Péter, Gábor Balogh, Zsolt Török, László Vígh, Attila Glatz
Cells.2024; 13(18): 1543. CrossRef - Lipid droplets: a cellular organelle vital in cancer cells
Yi Jin, Yanjie Tan, Jian Wu, Zhuqing Ren
Cell Death Discovery.2023;[Epub] CrossRef - Oleaginous yeasts: Time to rethink the definition?
José Manuel Salvador López, Meriam Vandeputte, Inge N. A. Van Bogaert
Yeast.2022; 39(11-12): 553. CrossRef - Proteomic and lipidomic analyses of lipid droplets in Aurantiochytrium limacinum ATCC MYA-1381
Kohei Yoneda, Yohei Ishibashi, Masaki Yoshida, Makoto M. Watanabe, Makoto Ito, Iwane Suzuki
Algal Research.2022; 67: 102844. CrossRef - ER-localized phosphatidylethanolamine synthase plays a conserved role in lipid droplet formation
Mehmet Oguz Gok, Natalie Ortiz Speer, W. Mike Henne, Jonathan R. Friedman, James Olzmann
Molecular Biology of the Cell.2022;[Epub] CrossRef - Lipid Droplet Nucleation
Abdou Rachid Thiam, Elina Ikonen
Trends in Cell Biology.2021; 31(2): 108. CrossRef - Metabolism of Storage Lipids and the Role of Lipid Droplets in the Yeast Schizosaccharomyces pombe
Ivan Hapala, Peter Griac, Roman Holic
Lipids.2020; 55(5): 513. CrossRef - Lipid Droplets in Neurodegenerative Disorders
Brandon C. Farmer, Adeline E. Walsh, Jude C. Kluemper, Lance A. Johnson
Frontiers in Neuroscience.2020;[Epub] CrossRef - Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts
Neha Arora, Hong-Wei Yen, George P. Philippidis
Sustainability.2020; 12(12): 5125. CrossRef - Mechanisms of protein targeting to lipid droplets: A unified cell biological and biophysical perspective
Ravi Dhiman, Stefanie Caesar, Abdou Rachid Thiam, Bianca Schrul
Seminars in Cell & Developmental Biology.2020; 108: 4. CrossRef - The New Face of the Lipid Droplet: Lipid Droplet Proteins
Congyan Zhang, Pingsheng Liu
PROTEOMICS.2019;[Epub] CrossRef - Effect of Selenium on Lipid and Amino Acid Metabolism in Yeast Cells
Marek Kieliszek, Stanisław Błażejak, Anna Bzducha-Wróbel, Anna M. Kot
Biological Trace Element Research.2019; 187(1): 316. CrossRef - The Peroxygenase Activity of the Aspergillus flavus Caleosin, AfPXG, Modulates the Biosynthesis of Aflatoxins and Their Trafficking and Extracellular Secretion via Lipid Droplets
Abdulsamie Hanano, Mari Alkara, Ibrahem Almousally, Mouhnad Shaban, Farzana Rahman, Mehedi Hassan, Denis J. Murphy
Frontiers in Microbiology.2018;[Epub] CrossRef - Mitotic defects in fission yeast lipid metabolism ‘cut’ mutants are suppressed by ammonium chloride
Róbert Zach, Jarmila Tvarůžková, Martin Schätz, Ondřej Ťupa, Beáta Grallert, Martin Převorovský
FEMS Yeast Research.2018;[Epub] CrossRef - Lipid Droplets: Formation to Breakdown
Alex Meyers, Taylor M. Weiskittel, Paul Dalhaimer
Lipids.2017; 52(6): 465. CrossRef
|
MSK
