Skip Navigation
Skip to contents

Journal of Microbiology : Journal of Microbiology

OPEN ACCESS
SEARCH
Search
Advanced Search
mobile menu button

Articles

Page Path
HOME > J. Microbiol > Volume 56(11); 2018 > Article
Journal Article
Identification and heterologous reconstitution of a 5-alk(en)ylresorcinol synthase from endophytic fungus Shiraia sp. Slf14
Huiwen Yan 1,2, Lei Sun 1, Jinge Huang 1, Yixing Qiu 1,3, Fuchao Xu 1, Riming Yan 1,4, Du Zhu 4, Wei Wang 3, Jixun Zhan 1,3
Journal of Microbiology 2018;56(11):805-812.
DOI: https://doi.org/10.1007/s12275-018-8278-x
Published online: October 24, 2018
1Department of Biological Engineering, Utah State University, Logan, UT 84322-4105, USA, 2The State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Hunan 410083, P. R. China, 3TCM and Ethnomedicine Innovation & Development Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Hunan 410208, P. R. China, 4Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, College of Life Science, Jiangxi Normal University, Jiangxi 330022, P. R. China
Corresponding author:  Wei Wang , Tel: +1-435-797-8774, 
Jixun Zhan , Tel: +1-435-797-8774, 
Received: 23 May 2018   • Revised: 13 July 2018   • Accepted: 16 July 2018
prev next
  • 101 Views
  • 0 Download
  • 8 Crossref
  • 9 Scopus
Full Article

Abstract

A new type III polyketide synthase gene (Ssars) was discovered from the genome of Shiraia sp. Slf14, an endophytic fungal strain from Huperzia serrata. The intron-free gene was cloned from the cDNA and ligated to two expression vectors pET28a and YEpADH2p-URA3 for expression in Escherichia coli BL21(DE3) and Saccharomyces cerevisiae BJ5464, respectively. SsARS was efficiently expressed in E. coli BL21(DE3), leading to the synthesis of a series of polyketide products. Six major products were isolated from the engineered E. coli and characterized as 1,3-dihydroxyphenyl- 5-undecane, 1,3-dihydroxyphenyl-5-cis-6􍿁-tridecene,1,3-dihydroxyphenyl- 5-tridecane, 1,3-dihydroxyphenyl-5-cis-8􍿁- pentadecene, 1,3-dihydroxyphenyl-5-pentadecane, and 1,3- dihydroxyphenyl-5-cis-10􍿁-heptadecene, respectively, based on the spectral data and biosynthetic origin. Expression of SsARS in the yeast also led to the synthesis of the same polyketide products, indicating that this enzyme can be reconstituted in both heterologous hosts. Supplementation of soybean oil into the culture of E. coli BL21(DE3)/SsARS increased the production titers of 1–6 and led to the synthesis of an additional product, which was identified as 5-(8􍿁Z,11􍿁Z-heptadecadienyl) resorcinol. This work thus allowed the identification of SsARS as a 5-alk(en)ylresorcinol synthase with flexible substrate specificity toward endogenous and exogenous fatty acids. Desired resorcinol derivatives may be synthesized by supplying corresponding fatty acids into the culture medium.

  • Cite this Article
    Cite this Article
    export Copy Download
    Close
    Download Citation
    Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.
    Format:
    • RIS — For EndNote, ProCite, RefWorks, and most other reference management software
    • BibTeX — For JabRef, BibDesk, and other BibTeX-specific software
    Include:
    • Citation for the content below
    Identification and heterologous reconstitution of a 5-alk(en)ylresorcinol synthase from endophytic fungus Shiraia sp. Slf14
    J. Microbiol. 2018;56(11):805-812.   Published online October 24, 2018
    DOI: https://doi.org/10.1007/s12275-018-8278-x
    Close
Related articles
Journal of Microbiology : Journal of Microbiology
© The Microbiological Society of Korea.
TOP