The hrp pathogenicity island of Pseudomonas syringae pv. tomato DC3000 is induced by plant phenolic acids

Jun Seung Lee; Hye Ryun Ryu; Ji Young Cha; Hyung Suk Baik

doi:10.1007/s12275-015-5256-4

Articles

Page Path: HOME > J. Microbiol > Volume 53(10); 2015 > Article

Research Support, Non-U.S. Gov't
The hrp pathogenicity island of Pseudomonas syringae pv. tomato DC3000 is induced by plant phenolic acids: Jun Seung Lee ¹, Hye Ryun Ryu ², Ji Young Cha ³, Hyung Suk Baik ¹; Journal of Microbiology 2015;53(10):725-731.
DOI: https://doi.org/10.1007/s12275-015-5256-4
Published online: October 2, 2015

¹Department of Integrated Biological Science, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea, ²R & D, CJ Cheil Jedang Corporation, Seoul 04560, Republic of Korea, ³Department of Biotechnology and Bioengineering, College of Engineering, Dong-Eui University, Busan 47340, Republic of Korea¹Department of Integrated Biological Science, College of Natural Sciences, Pusan National University, Busan 46241, Republic of Korea, ²R & D, CJ Cheil Jedang Corporation, Seoul 04560, Republic of Korea, ³Department of Biotechnology and Bioengineering, College of Engineering, Dong-Eui University, Busan 47340, Republic of Korea

Corresponding author: Hyung Suk Baik , Tel: +82-51-510-2271,

Received: 18 May 2015 • Revised: 8 September 2015 • Accepted: 15 September 2015

prev next

58 Views
0 Download
6 Crossref
6 Scopus

Full Article

Abstract

Plants produce a wide array of antimicrobial compounds, such as phenolic compounds, to combat microbial pathogens. The hrp PAI is one of the major virulence factors in the plant pathogen, Pseudomonas syringae. A major role of hrp PAI is to disable the plant defense system during bacterial invasion. We examined the influence of phenolic compounds on hrp PAI gene expression at low and high concentrations. There was approximately 2.5 times more hrpA and hrpZ mRNA in PtoDC3000 that was grown in minimal media (MM) supplemented with 10 μM of ortho-coumaric acid than in PtoDC3000 grown in MM alone. On the other hand, a significantly lower amount of hrpA mRNA was observed in bacteria grown in MM supplemented with a high concentration of phenolic compounds. To determine the regulation pathway for hrp PAI gene expression, we performed qRTPCR using gacS, gacA, and hrpS deletion mutants.

Keywords: plant phenolic compounds;Pseudomonas syringae;plant-pathogen interaction;hrp PAI;qRT-PCR

Figure & Data

References

Citations

Citations to this article as recorded by

Liebig review: The role of mineral nutrients in the development of Pseudomonas syringae diseases—Lessons learned and implications for disease control in woody plants
Nathalie Soethe, Michelle T. Hulin, Antje Balasus, Gail Preston, Christoph‐Martin Geilfus
Journal of Plant Nutrition and Soil Science.2024; 187(3): 301. CrossRef
Regulation of the Pseudomonas syringae Type III Secretion System by Host Environment Signals
Megan R. O’Malley, Jeffrey C. Anderson
Microorganisms.2021; 9(6): 1227. CrossRef
Quantification of Viable Cells of Pseudomonas syringae pv. tomato in Tomato Seed Using Propidium Monoazide and a Real-Time PCR Assay
A-li Chai, Hai-yan Ben, Wei-tao Guo, Yan-xia Shi, Xue-wen Xie, Lei Li, Bao-ju Li
Plant Disease.2020; 104(8): 2225. CrossRef
Validation of RT-qPCR Approaches to MonitorPseudomonas syringaeGene Expression During Infection and Exposure to Pattern-Triggered Immunity
Amy Smith, Amelia H. Lovelace, Brian H. Kvitko
Molecular Plant-Microbe Interactions®.2018; 31(4): 410. CrossRef
Multidrug Efflux Pumps at the Crossroad between Antibiotic Resistance and Bacterial Virulence
Manuel Alcalde-Rico, Sara Hernando-Amado, Paula Blanco, José L. Martínez
Frontiers in Microbiology.2016;[Epub] CrossRef
Global Analysis of Type Three Secretion System and Quorum Sensing Inhibition of Pseudomonas savastanoi by Polyphenols Extracts from Vegetable Residues
Carola Biancalani, Matteo Cerboneschi, Francesco Tadini-Buoninsegni, Margherita Campo, Arianna Scardigli, Annalisa Romani, Stefania Tegli, Boris Alexander Vinatzer
PLOS ONE.2016; 11(9): e0163357. CrossRef

Cite this Article

Cite this Article: export Copy Download Format; Close

Download Citation

Download a citation file in RIS format that can be imported by all major citation management software, including EndNote, ProCite, RefWorks, and Reference Manager.

Format:

RIS — For EndNote, ProCite, RefWorks, and most other reference management software
BibTeX — For JabRef, BibDesk, and other BibTeX-specific software

Include:

Citation for the content below
Citation and abstract for the content below

The hrp pathogenicity island of Pseudomonas syringae pv. tomato DC3000 is induced by plant phenolic acids

J. Microbiol. 2015;53(10):725-731. Published online October 2, 2015

DOI: https://doi.org/10.1007/s12275-015-5256-4

Related articles