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Effect of exopolysaccharides of Paenibacillus polymyxa rhizobacteria on physiological and morphological variables of wheat seedlings
Irina V. Yegorenkova , Kristina V. Tregubova , Alexander I. Krasov , Nina V. Evseeva , Larisa Yu. Matora
J. Microbiol. 2021;59(8):729-735.   Published online July 24, 2021
DOI: https://doi.org/10.1007/s12275-021-0623-9
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AbstractAbstract
Paenibacillus polymyxa is a promising plant-growth-promoting rhizobacterium that associates with a wide range of host plants, including agronomically important ones. Inoculation of wheat seedlings with P. polymyxa strains CCM 1465 and 92 was found to increase the mitotic index of the root cells 1.2- and 1.6-fold, respectively. Treatment of seedlings with the exopolysaccharides (EPSs) of these strains increased the mitotic index 1.9-fold (P. polymyxa CCM 1465) and 2.8-fold (P. polymyxa 92). These increases indicate activation of cell division in the root meristems. Analysis of the morphometric variables of the seedlings showed that P. polymyxa CCM 1465, P. polymyxa 92, and their EPSs promoted wheat growth, increasing root and shoot length up to 22% and root and shoot dry weight up to 28%, as compared with the control. In addition, both strains were found to intensely colonize the seedling root surface. Thus, P. polymyxa EPSs are active metabolites that, along with whole cells, are responsible for the contact interactions of the bacteria with wheat roots and are implicated in the induction of plant responses to these interactions. The strains used in this work are of interest for further study to broaden the existing understanding of the mechanisms of plant–bacterial interactions and to develop effective biofertilizers for agricultural purposes.

Citations

Citations to this article as recorded by  
  • Optimization of the Production Parameters of a Novel Exopolysaccharide Chrysosporine from Endophytic Chrysosporium sp. KTL2, and Evaluation of its Antioxidative and Prebiotic Potentialities
    Hiran Kanti Santra, Debdulal Banerjee
    Indian Journal of Microbiology.2024;[Epub]     CrossRef
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    Olga Tsivileva, Andrei Shaternikov, Nina Evseeva
    International Journal of Molecular Sciences.2024; 25(13): 6877.     CrossRef
  • Exopolysaccharides of Paenibacillus polymyxa: A review
    Xuan-Ya Huang, Xin-Pei Ye, Yan-Yu Hu, Zhen-Xing Tang, Tian Zhang, Hai Zhou, Ting Zhou, Xue-Lian Bai, Er-Xu Pi, Bing-Hua Xie, Lu-E Shi
    International Journal of Biological Macromolecules.2024; 261: 129663.     CrossRef
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Research Support, Non-U.S. Gov'ts
Characterization and Screening of Plant Probiotic Traits of Bacteria Isolated from Rice Seeds Cultivated in Argentina
Dante Ruiza , Betina Agaras , Patrice de Werrab , Luis G. Wall , Claudio Valverde
J. Microbiol. 2011;49(6):902-912.   Published online December 28, 2011
DOI: https://doi.org/10.1007/s12275-011-1073-6
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AbstractAbstract
Many seeds carry endophytes, which ensure good chances of seedling colonization. In this work, we have studied the seed-borne bacterial flora of rice varieties cultivated in the northeast of Argentina. Surface-sterilized husked seeds of the rice cultivars CT6919, El Paso 144, CAMBA, and IRGA 417 contained an average of 5×106 CFU/g of mesophilic and copiotrophic bacteria. Microbiological, physiological, and molecular characterization of a set of 39 fast-growing isolates from the CT6919 seeds revealed an important diversity of seed-borne mesophiles and potential plant probiotic activities, including diazotrophy and antagonism of fungal pathogens. In fact, the seed-borne bacterial flora protected the rice seedlings against Curvularia sp. infection. The root colonization pattern of 2 Pantoea isolates from the seeds was studied by fluorescence microscopy of the inoculated axenic rice seedlings. Both isolates strongly colonized the site of emergence of the lateral roots and lenticels, which may represent the entry sites for endophytic spreading. These findings suggest that rice plants allow grain colonization by bacterial species that may act as natural biofertilizers and bioprotectives early from seed germination.

Citations

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Gibberellins-Producing Rhizobacteria Increase Endogenous Gibberellins Content and Promote Growth of Red Peppers
Gil-Jae Joo , Young-Mog Kim , Jung-Tae Kim , In-Koo Rhee , Jin-Ho Kim , In-Jung Lee
J. Microbiol. 2005;43(6):510-515.
DOI: https://doi.org/2297 [pii]
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AbstractAbstract
The growth of red pepper plants was enhanced by treatment with the rhizobacterium, Bacillus cereus MJ-1. Red pepper shoots showed a 1.38-fold increase in fresh weight (fw) and roots showed a 1.28-fold fw gain. This plant growth-promoting rhizobacterium (PGPR) has been reported to produce gibberellins (GAs). Other GAs-producing rhizobacteria, Bacillus macroides CJ-29 and Bacillus pumilus CJ-69, also enhanced the fw of the plants. They were less effective than B. cereus MJ-1, though. The endogenous GAs content of pepper shoots inoculated with MJ-1 was also higher than in shoots inoculated with CJ-29 or CJ-69. When inoculated with MJ-1, bacterial colonization rate of the roots was higher than that of roots inoculated with CJ-29 or CJ-69. These results support the idea that the plant growth-promoting effect of the bacteria also positively related with the efficiency of root colonization by the bacteria. In addition, we identified the major endogenous GAs of the red pepper as originating from both the early C-13 hydroxylation and the early non C-13 hydroxylation pathways, with the latter being the predominant pathway of GA biosynthesis in red pepper shoots.

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