Journal of Microbiology

Journal Article

Transcriptome‑based Mining of the Constitutive Promoters for Tuning Gene Expression in Aspergillus oryzae: Kobkul Laoteng , Jutamas Anantayanon , Chanikul Chutrakul , Sarocha Panchanawaporn , Sukanya Jeennor; J. Microbiol. 2023;61(2):199-210. Published online February 6, 2023; DOI: https://doi.org/10.1007/s12275-023-00020-0

431 View
3 Download
8 Web of Science
7 Crossref

Transcriptional regulation has been adopted for developing metabolic engineering tools. The regulatory promoter is a crucial genetic element for strain optimization. In this study, a gene set of Aspergillus oryzae with highly constitutive expression across different growth stages was identified through transcriptome data analysis. The candidate promoters were functionally characterized in A. oryzae by transcriptional control of β-glucuronidase (GUS) as a reporter. The results showed that the glyceraldehyde triphosphate dehydrogenase promoter (PgpdA1) of A. oryzae with a unique structure displayed the most robust strength in constitutively controlling the expression compared to the PgpdA2 and other putative promoters tested. In addition, the ubiquitin promoter (Pubi) of A. oryzae exhibited a moderate expression strength. The deletion analysis revealed that the 5' untranslated regions of gpdA1 and ubi with the length of 1028 and 811 nucleotides, counted from the putative translation start site (ATG), respectively, could efficiently drive the GUS expression. Interestingly, both promoters could function on various carbon sources for cell growth. Glucose was the best fermentable carbon source for allocating high constitutive expressions during cell growth, and the high concentrations (6–8% glucose, w/v) did not repress their functions. It was also demonstrated that the secondary metabolite gene coding for indigoidine could express under the control of PgpdA1 or Pubi promoter. These strong and moderate promoters of A. oryzae provided beneficial options in tuning the transcriptional expression for leveraging the metabolic control towards the targeted products.

Citations

Citations to this article as recorded by

Construction of an Aspergillus oryzae △nptB△pyrG Host for Homologous Expression of Lipase and Catalytic Property Characterization of Recombinant Lipase
Yueting Zhang, Hongmei Nie, Fei Zhang, Mengmeng Jin, Zhao Wang, Jianyong Zheng
Applied Biochemistry and Biotechnology.2025; 197(2): 873. CrossRef
Transcriptome-Based Mining of the Strong Promoters for Hyperproduction of Gibberellin GA3 in Fusarium fujikuroi
Qi Guo, Yue-Feng Zhong, Xin-Yu Chen, Ya-Wen Li, Yu-Xin Yang, Zhi-Kui Nie, Tian-Qiong Shi
Journal of Agricultural and Food Chemistry.2025; 73(14): 8440. CrossRef
Promoter engineering of filamentous fungi for novel natural product discovery
Xiangzhou Gong, Jing Tian, Huawei Zhang
Bioorganic Chemistry.2025; 163: 108798. CrossRef
Mining and Understanding of New Transcriptional Regulatory Elements from Licorice-Derived Endophyte Serratia Rubidaea W12-1
Ying Zhang, Yunyang Ma, Bing Hu, H.M. Zabed, A.K. Singh, M.A. Ibrahim, N. Chen
BIO Web of Conferences.2024; 142: 03018. CrossRef
Development of Aspergillus oryzae BCC7051 as a Robust Cell Factory Towards the Transcriptional Regulation of Protease-Encoding Genes for Industrial Applications
Sarocha Panchanawaporn, Chanikul Chutrakul, Sukanya Jeennor, Jutamas Anantayanon, Kobkul Laoteng
Journal of Fungi.2024; 11(1): 6. CrossRef
Exploring and Engineering Novel Strong Promoters for High-Level Protein Expression in Bacillus subtilis DB104 through Transcriptome Analysis
Ji-Su Jun, Hyang-Eun Jeong, Kwang-Won Hong
Microorganisms.2023; 11(12): 2929. CrossRef
Efficient de novo production of bioactive cordycepin by Aspergillus oryzae using a food-grade expression platform
Sukanya Jeennor, Jutamas Anantayanon, Sarocha Panchanawaporn, Chanikul Chutrakul, Wanwipa Vongsangnak, Kobkul Laoteng
Microbial Cell Factories.2023;[Epub] CrossRef

Research Support, Non-U.S. Gov't

The Pectate Lyase Encoded by the pecCl1 Gene Is an Important Determinant for the Aggressiveness of Colletotrichum lindemuthianum: Andréia Cnossen-Fassoni , Denise Mara Soares Bazzolli , Sérgio Hermínio Brommonschenkel , Elza Fernandes de Araújo , Marisa Vieira de Queiroz; J. Microbiol. 2013;51(4):461-470. Published online August 30, 2013; DOI: https://doi.org/10.1007/s12275-013-3078-9

321 View
0 Download
14 Crossref

Abstract PDF

Colletotrichum lindemuthianum is the causal agent of anthracnose in the common bean, and the genes that encode its cell-wall-degrading enzymes are crucial for the development of the disease. Pectinases are the most important group of cell wall-degrading enzymes produced by phytopathogenic fungi. The pecC1l gene, which encodes a pectate lyase in C. lindemuthianum, was isolated and characterized. Possible cis-regulatory elements and transcription factor binding sites that may be involved in the regulation of genetic expression were detected in the promoter region of the gene. pecCl1 is represented by a single copy in the genome of C. lindemuthianum, though in silico analyses of the genomes of Colletotrichum graminicola and Colletotrichum higginsianum suggest that the genome of C. lindemuthianum includes other genes that encode pectate lyases. Phylogenetic analysis detected two groups that clustered based on different members of the pectate lyase family. Analysis of the differential expression of pecCl1 during different stages of infection showed a significant increase in pecCl1 expression five days after infection, at the onset of the necrotrophic phase. The split-maker technique proved to be an efficient method for inactivation of the pecCl1 gene, which allowed functional study of a mutant with a site-specific integration. Though gene inactivation did not result in complete loss of pectate lyase activity, the symptoms of anthracnose were reduced. Analysis of pectate lyases might not only contribute to the understanding of anthracnose in the common bean but might also lead to the discovery of an additional target for controlling anthracnose.

Citations

Citations to this article as recorded by

What lies behind the large genome of Colletotrichum lindemuthianum
Leandro Lopes da Silva, Hilberty Lucas Nunes Correia, Osiel Silva Gonçalves, Pedro Marcus Pereira Vidigal, Rafael Oliveira Rosa, Mateus Ferreira Santana, Marisa Vieira de Queiroz
Frontiers in Fungal Biology.2024;[Epub] CrossRef
Functional genomics identifies a small secreted protein that plays a role during the biotrophic to necrotrophic shift in the root rot pathogen Phytophthora medicaginis
Donovin W. Coles, Sean L. Bithell, Thomas Jeffries, William S. Cuddy, Jonathan M. Plett
Frontiers in Plant Science.2024;[Epub] CrossRef
Differential Carbon Catabolite Repression and Hemicellulolytic Ability among Pathotypes of Colletotrichum lindemuthianum against Natural Plant Substrates
Karla Morelia Díaz-Tapia, María Guadalupe Zavala-Páramo, Maria Guadalupe Villa-Rivera, Ma. Irene Morelos-Martínez, Everardo López-Romero, June Simpson, Jeni Bolaños-Rebolledo, Horacio Cano-Camacho
Journal of Fungi.2024; 10(6): 406. CrossRef
A plant cell death-inducing protein from litchi interacts with Peronophythora litchii pectate lyase and enhances plant resistance
Wen Li, Peng Li, Yizhen Deng, Junjian Situ, Zhuoyuan He, Wenzhe Zhou, Minhui Li, Pinggen Xi, Xiangxiu Liang, Guanghui Kong, Zide Jiang
Nature Communications.2024;[Epub] CrossRef
Pectate Lyase Genes Abundantly Expressed During the Infection Regulate Morphological Development of Colletotrichum camelliae and CcPEL16 Is Required for Full Virulence to Tea Plants
Hong Jiang, Qinghai Cao, Xinchao Wang, Wuyun Lv, Yuchun Wang, Aaron P. Mitchell
mSphere.2023;[Epub] CrossRef
Pectinolytic arsenal of Colletotrichum lindemuthianum and other fungi with different lifestyles
Leandro Lopes da Silva, Túlio Morgan, Ediones Amaro Garcia, Rafael Oliveira Rosa, Tiago Antônio de Oliveira Mendes, Marisa Vieira de Queiroz
Journal of Applied Microbiology.2022; 133(3): 1857. CrossRef
Use of Agrobacterium tumefaciens-mediated transformation for the inactivation of pectinase genes in Colletotrichum lindemuthianum
Leandro Lopes da Silva, Rafael Oliveira Rosa, Ediones Amaro Garcia, Janaina Aparecida Teixeira, Tiago Antônio de Oliveira Mendes, Marisa Vieira de Queiroz
European Journal of Plant Pathology.2022; 162(3): 595. CrossRef
Gene deletion and constitutive expression of the pectate lyase gene 1 (MoPL1) lead to diminished virulence of Magnaporthe oryzae
Alex Wegner, Florencia Casanova, Marco Loehrer, Angelina Jordine, Stefan Bohnert, Xinyu Liu, Zhengguang Zhang, Ulrich Schaffrath
Journal of Microbiology.2022; 60(1): 79. CrossRef
Whole Genome Sequence of Alternaria alternata, the Causal Agent of Black Spot of Kiwifruit
Ke Huang, Jianming Tang, Yong Zou, Xiangcheng Sun, Jianbin Lan, Wei Wang, Panpan Xu, Xiangwei Wu, Rui Ma, Qi Wang, Zhenshuo Wang, Jia Liu
Frontiers in Microbiology.2021;[Epub] CrossRef
Identification and characterization of pectin lyase gene as a virulence factor in Colletotrichum gloeosporioides
Kiran S. Chudasama, Jagdishchandra K. Monpara, Vrinda S. Thaker
Physiological and Molecular Plant Pathology.2021; 116: 101706. CrossRef
Genome-wide association study of partial resistance to sclerotinia stem rot of cultivated soybean based on the detached leaf method
Mingming Sun, Yan Jing, Xue Zhao, Weili Teng, Lijuan Qiu, Hongkun Zheng, Wenbin Li, Yingpeng Han, Istvan Rajcan
PLOS ONE.2020; 15(5): e0233366. CrossRef
Functions and mechanisms: polygalacturonases from plant pathogenic fungi as pathogenicity and virulence factors
Masayuki Nakamura, Hisashi Iwai
Journal of General Plant Pathology.2019; 85(4): 243. CrossRef
Spotlight on fungal pectin utilization—from phytopathogenicity to molecular recognition and industrial applications
Kevin Schmitz, Ryan Protzko, Lisha Zhang, J. Philipp Benz
Applied Microbiology and Biotechnology.2019; 103(6): 2507. CrossRef
A Colletotrichum graminicola mutant deficient in the establishment of biotrophy reveals early transcriptional events in the maize anthracnose disease interaction
Maria F. Torres, Noushin Ghaffari, Ester A. S. Buiate, Neil Moore, Scott Schwartz, Charles D. Johnson, Lisa J. Vaillancourt
BMC Genomics.2016;[Epub] CrossRef

First
Prev
Page of 1
Next
Last

Search