Journal of Microbiology

Journal Articles

Antimicrobial Efficacy of Allium cepa and Zingiber officinale Against the Milk‑Borne Pathogen Listeria monocytogenes: Abirami Arasu , Nagaram Prabha , Durga Devi , Praveen Kumar Issac , Khaloud Mohammed Alarjani , Dunia A. Al Farraj , Reem A. Aljeidi , Dina S. Hussein , Magesh Mohan , Jehad Zuhair Tayyeb , Ajay Guru , Jesu Arockiaraj; J. Microbiol. 2023;61(11):993-1011. Published online December 4, 2023; DOI: https://doi.org/10.1007/s12275-023-00086-w

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Listeria monocytogenes is an important food-borne pathogen that causes listeriosis and has a high case fatality rate despite its low incidence. Medicinal plants and their secondary metabolites have been identified as potential antibacterial substances, serving as replacements for synthetic chemical compounds. The present studies emphasize two significant medicinal plants, Allium cepa and Zingiber officinale, and their efficacy against L. monocytogenes. Firstly, a bacterial isolate was obtained from milk and identified through morphology and biochemical reactions. The species of the isolate were further confirmed through 16S rRNA analysis. Furthermore, polar solvents such as methanol and ethanol were used for the extraction of secondary metabolites from A. cepa and Z. officinale. Crude phytochemical components were identified using phytochemical tests, FTIR, and GC–MS. Moreover, the antibacterial activity of the crude extract and its various concentrations were tested against L. monocytogenes. Among all, A. cepa in methanolic extracts showed significant inhibitory activity. Since, the A. cepa for methanolic crude extract was used to perform autography to assess its bactericidal activity. Subsequently, molecular docking was performed to determine the specific compound inhibition. The docking results revealed that four compounds displayed strong binding affinity with the virulence factor Listeriolysin-O of L. monocytogenes. Based on the above results, it can be concluded that the medicinal plant A. cepa has potential antibacterial effects against L. monocytogenes, particularly targeting its virulence.

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Cultural Perspectives on the Sustainable Use and Added Value of Plant-Based Food Dyes—A Case Study from Bulgaria
Mihail Chervenkov, Teodora Ivanova, Yulia Bosseva, Dessislava Dimitrova
Sustainability.2024; 16(20): 9049. CrossRef

Biofilm characterization of Fusarium solani keratitis isolate: increased resistance to antifungals and UV light: Itzel Margarita Córdova-Alcántara , Diana Laura Venegas-Cortés , María Ángeles Martínez-Rivera , Néstor Octavio Pérez , Aida Verónica Rodriguez-Tovar; J. Microbiol. 2019;57(6):485-497. Published online May 27, 2019; DOI: https://doi.org/10.1007/s12275-019-8637-2

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Abstract

Fusarium solani has drawn phytopathogenic, biotechnological, and medical interest. In humans, it is associated with localized infections, such as onychomycosis and keratomycosis, as well as invasive infections in immunocompromised patients. One pathogenicity factor of filamentous fungi is biofilm formation. There is still only scarce information about the in vitro mechanism of the formation and composition of F. solani biofilm. In this work, we describe the biofilm formed by a clinical keratomycosis isolate in terms of its development, composition and susceptibility to different antifungals and ultraviolet light (UV) at different biofilm formation stages. We found five biofilm formation stages using scanning electron microscopy: adherence, germination, hyphal development, maturation, and cell detachment. Using epifluorescence microscopy with specific fluorochromes, it was elucidated that the extracellular matrix consists of carbohydrates, proteins, and extracellular DNA. Specific inhibitors for these molecules showed significant biofilm reductions. The antifungal susceptibility against natamycin, voriconazole, caspofungin, and amphotericin B was evaluated by metabolic activity and crystal violet assay, with the F. solani biofilm preformation to 24 h increased in resistance to natamycin, voriconazole, and caspofungin, while the biofilm preformation to 48 h increased in resistance to amphotericin B. The preformed biofilm at 24 h protected and reduced UV light mortality. F. solani isolate could produce a highly structured extra biofilm; its cellular matrix consists of carbohydrate polymers, proteins, and eDNA. Biofilm confers antifungal resistance and decreases its susceptibility to UV light. The fungal biofilm functions as a survival strategy against antifungals and environmental factors.

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Trans-kingdom fungal pathogens infecting both plants and humans, and the problem of azole fungicide resistance
Alexandra Pintye, Renáta Bacsó, Gábor M. Kovács
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Biofilm Formation in Clinical Isolates of Fusarium
Ray Zhang, Nathan Wiederhold, Richard Calderone, Dongmei Li
Journal of Fungi.2024; 10(11): 766. CrossRef
Global research on keratomycosis: New insights from latent Dirichlet allocation and HJ-Biplot-driven knowledge mapping study
Julia Patricia Duran-Ospina, Javier de la Hoz-M, Naga Raju Maddela, German Josuet Lapo-Talledo, Aline Siteneski, Karime Montes-Escobar
Diagnostic Microbiology and Infectious Disease.2024; 110(1): 116442. CrossRef
Influence of the carbon-to-nitrogen ratio in the atrazine biodegradation from contaminated waters in submerged fermentation by free and immobilized Penicillium chrysogenum NRRL 807
Sinara Cybelle Turíbio e Silva-Nicodemo, Pedro Ferreira de Souza, Marina Moura Lima, Everaldo Silvino dos Santos, Gorete Ribeiro de Macêdo
Revista Brasileira de Ciências Ambientais.2024;[Epub] CrossRef
Revealing the regulatory impact of nutrient on the production of (R)-2-(4-Hydroxyphenoxy)propanoic acid by Beauveria bassiana biofilms through comparative transcriptomics analyse
Shuping Zou, Yizhi Ma, Lixiang Zhao, Xiaomin Chen, Hailing Gao, Juan Chen, Yaping Xue, Yuguo Zheng
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Fusarium Keratitis: A Systematic Review (1969 to 2023)
Maria Eduarda Carvalho Cintra, Maryanna da Silva Dantas, Abdullah M. S. Al-Hatmi, Rafael Wesley Bastos, Luana Rossato
Mycopathologia.2024;[Epub] CrossRef
Control of biofilms with UV light: a critical review of methodologies, research gaps, and future directions
Stephanie L. Gora, Ben Ma, Mariana Lanzarini-Lopes, Hamed Torkzadeh, Zhe Zhao, Christian Ley Matthews, Paul Westerhoff, Karl Linden, Benoit Barbeau, Rich Simons, Graham Gagnon, Patrick Di Falco, Muhammad Salman Mohsin
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Jesse Jorna, Byron J. Adams, Zachary T. Aanderud, Paul B. Frandsen, Cristina Takacs‐Vesbach, Sonia Kéfi
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Life on a leaf: the epiphyte to pathogen continuum and interplay in the phyllosphere
Graham Thomas, William T. Kay, Helen N. Fones
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Pseudolaric Acid B Ameliorates Fungal Keratitis Progression by Suppressing Inflammation and Reducing Fungal Load
Min Yin, Na Li, Lina Zhang, Jing Lin, Qian Wang, Lingwen Gu, Hengrui Zheng, Guiqiu Zhao, Cui Li
ACS Infectious Diseases.2023; 9(6): 1196. CrossRef
Microbial isolation and characterization from two flex lines from the urine processor assembly onboard the International Space Station
Hang Ngoc Nguyen, G. Marie Sharp, Sarah Stahl-Rommel, Yo-Ann Velez Justiniano, Christian L. Castro, Mayra Nelman-Gonzalez, Aubrie O’Rourke, Michael D. Lee, Jill Williamson, Chelsea McCool, Brian Crucian, Kenneth W. Clark, Miten Jain, Sarah L. Castro-Walla
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Arsenic and chromium resistance mechanisms in the Micrococcus luteus group
Ivan ARROYO-HERRERA, Brenda ROMÁN-PONCE, Rafael BUSTAMANTE-BRITO, Joseph GUEVARA-LUNA, Erika Yanet TAPIA-GARCÍA, Violeta LARIOS-SERRATO, Nannan ZHANG, Paulina ESTRADA-DE LOS SANTOS, En Tao WANG, María Soledad VÁSQUEZ-MURRIETA
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Differential Susceptibility of Mixed Polymicrobial Biofilms Involving Ocular Coccoid Bacteria (Staphylococcus aureus and S. epidermidis) and a Filamentous Fungus (Fusarium solani) on Ex Vivo Human Corneas
Sisinthy Shivaji, Banka Nagapriya, Konduri Ranjith
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Subinhibitory effects of 2,4-diacetylphloroglucinol on filamentous fungus Aspergillus fumigatus
Artyom A Stepanov, Anastasia V Vasilchenko, Alexey S Vasilchenko
Journal of Applied Microbiology.2023;[Epub] CrossRef
Evaluation of the performance of TiO2 thin films doped with silver nanoparticles as a protective coating for metal prostheses
Ohtokani Cabrera-Rodríguez, Martín Daniel Trejo-Valdez, Christopher René Torres-SanMiguel, Nury Pérez-Hernández, Ángel Bañuelos-Hernández, María Elena Manríquez-Ramírez, José Alejandro Hernández-Benítez, Aída Verónica Rodríguez-Tovar
Surface and Coatings Technology.2023; 458: 129349. CrossRef
Effect of Voriconazole on Biofilm of Filamentous Species Isolated from Keratitis
Mahsa Fattahi, Reza Ghasemi, Olga Pinegina, Mohammad Mahdi Bahrami, Mahdi Hosseini, Ensieh Lotfali
Archives of Clinical Infectious Diseases.2022;[Epub] CrossRef
Advances in application of ultraviolet irradiation for biofilm control in water and wastewater infrastructure
Xueru Luo, Baoping Zhang, Yinghua Lu, Yang Mei, Liang Shen
Journal of Hazardous Materials.2022; 421: 126682. CrossRef
Biofilm Formation by Chromoblastomycosis Fungi Fonsecaea pedrosoi and Phialophora verrucosa: Involvement with Antifungal Resistance
Ingrid S. Sousa, Thaís P. Mello, Elaine P. Pereira, Marcela Q. Granato, Celuta S. Alviano, André L. S. Santos, Lucimar F. Kneipp
Journal of Fungi.2022; 8(9): 963. CrossRef
In vitro ability of Fusarium keratoplasticum to form biofilms in venous catheter
Alana Salvador, Flávia Franco Veiga, Terezinha Inez Estivalet Svidzinski, Melyssa Negri
Microbial Pathogenesis.2022; 173: 105868. CrossRef
Fungal keratitis: Mechanisms of infection and management strategies
Christopher Donovan, Eduardo Arenas, Ramesh S Ayyala, Curtis E Margo, Edgar M. Espana
Survey of Ophthalmology.2022; 67(3): 758. CrossRef
Biofilm Formation and Structure in the Filamentous Fungus Fusarium graminearum, a Plant Pathogen
Rebecca Shay, Aaron A. Wiegand, Frances Trail, Yonglin Wang
Microbiology Spectrum.2022;[Epub] CrossRef
Comparison of cell viability assessment and visualization of Aspergillus niger biofilm with two fluorescent probe staining methods
Aswathy Shailaja, Terri F. Bruce, Patrick Gerard, Rhonda R. Powell, Charles A. Pettigrew, Julia L. Kerrigan
Biofilm.2022; 4: 100090. CrossRef
Update on diagnosis and management of refractory corneal infections
Shweta Agarwal, Tanveer A Khan, Murugesan Vanathi, Bhaskar Srinivasan, Geetha Iyer, Radhika Tandon
Indian Journal of Ophthalmology.2022; 70(5): 1475. CrossRef
Biofilm formation in clinically relevant filamentous fungi: a therapeutic challenge
Maryam Roudbary, Roya Vahedi-Shahandashti, André Luis Souza dos Santos, Shahla Roudbar Mohammadi, Peyman Aslani, Cornelia Lass-Flörl, Célia F. Rodrigues
Critical Reviews in Microbiology.2022; 48(2): 197. CrossRef
Species Diversity in the Fusarium solani (Neocosmospora) Complex and Their Pathogenicity for Plants and Humans
G. D. Sokolova, N. I. Budynkov, E. E. Tselipanova, A. P. Glinushkin
Doklady Biological Sciences.2022; 507(1): 416. CrossRef
Diagnosis and Management of Invasive Fungal Wound Infections in Burn Patients
Kaitlin A. Pruskowski, Thomas A. Mitchell, John L. Kiley, Trevor Wellington, Garrett W. Britton, Leopoldo C. Cancio
European Burn Journal.2021; 2(4): 168. CrossRef
Fusarium Keratitis—Review of Current Treatment Possibilities
Marek Szaliński, Aleksandra Zgryźniak, Izabela Rubisz, Małgorzata Gajdzis, Radosław Kaczmarek, Joanna Przeździecka-Dołyk
Journal of Clinical Medicine.2021; 10(23): 5468. CrossRef
Microbial Warfare on Three Fronts: Mixed Biofilm of Aspergillus fumigatus and Staphylococcus aureus on Primary Cultures of Human Limbo-Corneal Fibroblasts
Adrián Ramírez-Granillo, Luis Antonio Bautista-Hernández, Víctor Manuel Bautista-De Lucío, Fátima Sofía Magaña-Guerrero, Alfredo Domínguez-López, Itzel Margarita Córdova-Alcántara, Néstor O. Pérez, María de los Angeles Martínez-Rivera, Aída Verónica Rodrí
Frontiers in Cellular and Infection Microbiology.2021;[Epub] CrossRef
Recent Advances in Photodynamic Therapy against Fungal Keratitis
Jia-Horung Hung, Chaw-Ning Lee, Huai-Wen Hsu, I-Son Ng, Chi-Jung Wu, Chun-Keung Yu, Nan-Yao Lee, Yun Chang, Tak-Wah Wong
Pharmaceutics.2021; 13(12): 2011. CrossRef
The role of fungi in fungal keratitis
Bethany Mills, Naveen Radhakrishnan, Siva Ganesa Karthikeyan Rajapandian, Gunasekaran Rameshkumar, Prajna Lalitha, N. Venkatesh Prajna
Experimental Eye Research.2021; 202: 108372. CrossRef
Pathogenesis and Clinical Features of Fungal Keratitis (Review)
K. I. Belskaia, A. S. Obrubov
Ophthalmology in Russia.2021; 18(1): 12. CrossRef
Advances in Fusarium drug resistance research
Busi Zhao, Dan He, Li Wang
Journal of Global Antimicrobial Resistance.2021; 24: 215. CrossRef
Fungal Biofilms as a Valuable Target for the Discovery of Natural Products That Cope with the Resistance of Medically Important Fungi—Latest Findings
Estefanía Butassi, Laura Svetaz, María Cecilia Carpinella, Thomas Efferth, Susana Zacchino
Antibiotics.2021; 10(9): 1053. CrossRef
A Paradigm Shift in the Treatment and Management of Onychomycosis
Aditya K. Gupta, Maanasa Venkataraman, Helen J. Renaud, Richard Summerbell, Neil H. Shear, Vincent Piguet
Skin Appendage Disorders.2021; 7(5): 351. CrossRef
Critical parameters in cultivation of experimental biofilms using the example of Pseudomonas fluorescens
Kirsten Reddersen, André Güllmar, Silke Tonndorf-Martini, Bernd W. Sigusch, Andrea Ewald, Thomas J. Dauben, Karin Martin, Cornelia Wiegand
Journal of Materials Science: Materials in Medicine.2021;[Epub] CrossRef
A One Health Perspective to Recognize Fusarium as Important in Clinical Practice
Valeri Sáenz, Carlos Alvarez-Moreno, Patrice Le Pape, Silvia Restrepo, Josep Guarro, Adriana Marcela Celis Ramírez
Journal of Fungi.2020; 6(4): 235. CrossRef
Ethylenediaminetetraacetic Acid Disodium Salt Acts as an Antifungal Candidate Molecule against Fusarium graminearum by Inhibiting DON Biosynthesis and Chitin Synthase Activity
Xiu-Shi Song, Kai-Xin Gu, Jing Gao, Jian-Xin Wang, Shao-Chen Ding, Mingguo Zhou
Toxins.2020; 13(1): 17. CrossRef
Synergistic association of clioquinol with antifungal drugs against biofilm forms of clinical Fusarium isolates
Magda Antunes de Chaves, Thaís Ferreira do Amaral, Natália Monteiro da Silva Rodrigues Coutinho, Taís Fernanda Andrzejewski Kaminski, Mário Lettieri Teixeira, Luís Flavio Souza de Oliveira, Saulo Fernandes de Andrade, Alexandre Meneghello Fuentefria
Mycoses.2020; 63(10): 1069. CrossRef
Bibliometric and visualized analysis of global research on fungal keratitis from 1959 to 2019
Yujie Cen, Yingyu Li, Chen Huang, Wei Wang
Medicine.2020; 99(22): e20420. CrossRef
Negative interaction of Staphylococcus aureus on Fusarium falciforme growth ocular isolates in an in vitro mixed biofilm
Luis Antonio Bautista-Hernández, José Luis Gómez-Olivares, Beatriz Buentello-Volante, Alfredo Dominguez-Lopez, Yonathan Garfias, María Cristina Acosta-García, Rosa Paulina Calvillo-Medina, Victor Manuel Bautista-de Lucio
Microbial Pathogenesis.2019; 135: 103644. CrossRef

Fluorescence change of Fusobacterium nucleatum due to Porphyromonas gingivalis: Min-Ah Lee , Si-Mook Kang , Se-Yeon Kim , Ji-Soo Kim , Jin-Bom Kim , Seung-Hwa Jeong; J. Microbiol. 2018;56(9):628-633. Published online August 23, 2018; DOI: https://doi.org/10.1007/s12275-018-7515-7

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Abstract

The aim of this study was to measure changes in the fluorescence of Fusobacterium nucleatum interacting with Porphyromonas gingivalis for excitation with blue light at 405-nm. P. gingivalis was mono- and co-cultivated in close proximity with F. nucleatum. The fluorescence of the bacterial colonies was photographed using a QLF-D (Quantitative Light-induced Fluorescence-Digital) Biluminator camera system with a 405 nm light source and a specific filter. The red, green and blue intensities of fluorescence images were analyzed using the image analysis software. A fluorescence spectrometer was used to detect porphyrin synthesized by each bacterium. F. nucleatum, which emitted green fluorescence in single cultures, showed intense red fluorescence when it was grown in close proximity with P. gingivalis. F. nucleatum co-cultivated with P. gingivalis showed the same pattern of fluorescence peaks as for protoporphyrin IX in the red part of the spectrum. We conclude that the green fluorescence of F. nucleatum can change to red fluorescence in the presence of adjacent co-cultured with P. gingivalis, indicating that the fluorescence character of each bacterium might depend on the presence of other bacteria.

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Red/Orange Autofluorescence in Selected Candida Strains Exposed to 405 nm Laser Light
Rafał Wiench, Dariusz Paliga, Anna Mertas, Elżbieta Bobela, Anna Kuśka-Kiełbratowska, Sonia Bordin-Aykroyd, Aleksandra Kawczyk-Krupka, Kinga Grzech-Leśniak, Monika Lukomska-Szymanska, Edward Lynch, Dariusz Skaba
Dentistry Journal.2024; 12(3): 48. CrossRef
Autofluorescence Detection Method for Dental Plaque Bacteria Detection and Classification: Example of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Streptococcus mutans
Yung-Jhe Yan, Bo-Wen Wang, Chih-Man Yang, Ching-Yi Wu, Mang Ou-Yang
Dentistry Journal.2021; 9(7): 74. CrossRef
Fluorescence image and microbiological analysis of biofilm retained around healthy and inflamed orthodontic miniscrews
A.S. Garcez, L.C. Barros, M.R.U. Fernandes, D.N. Fujii, S.S. Suzuki, R. Nepomuceno
Photodiagnosis and Photodynamic Therapy.2020; 30: 101707. CrossRef

Morphologies and phenotypes in Bacillus subtilis biofilms: Xiaoling Wang , Shuo Meng , Jingshi Han; J. Microbiol. 2017;55(8):619-627. Published online July 4, 2017; DOI: https://doi.org/10.1007/s12275-017-7041-z

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Abstract

In this study, we explored Bacillus subtilis biofilm growth under various conditions such as the use of substrates with different stiffnesses and nutrient levels using a well-developed optical imaging technique to spatially and temporally track biofilm growth. We also developed a quantitative method to characterize B. subtilis biofilm morphologies under various growth conditions. To determine biofilm rim irregularities, we used the dimensionless P2A ratio, defined as P2/4πA, where P is the perimeter and A is the area of the biofilm. To estimate biofilm thickness from transmission images, we developed a calibration procedure based on Beer- Lambert’s law and cross sectioning. Furthermore, to determine the distributions of different B. subtilis cell phenotypes during biofilm growth, we used a triple-fluorescence-labeled B. subtilis strain that expressed motility, matrix production, and sporulation. Based on this work, we are able to tune biofilm growth by changing its growing environment.

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Analysis of biofilm expansion rate of Bacillus subtilis (MTC871) on agar substrates with different stiffness
Jin Wu, Xianyong Li, Rui Kong, Jiankun Wang, Xiaoling Wang
Canadian Journal of Microbiology.2023; 69(12): 479. CrossRef
In vitro studies of biofilm-forming Bacillus strains, biocontrol agents isolated from the maize phyllosphere
Aluminé Fessia, Melina Sartori, Daiana García, Luciana Fernández, Rodrigo Ponzio, Germán Barros, Andrea Nesci
Biofilm.2022; 4: 100097. CrossRef
Bistability and Formation of the Biofilm Matrix as Adaptive Mechanisms during the Stationary Phase of Bacillus subtilis
M. R. Sharipova, A. M. Mardanova, N. L. Rudakova, D. S. Pudova
Microbiology.2021; 90(1): 20. CrossRef
Effect of Viscosity on Microswimmers: A Comparative Study
Audrey Nsamela, Priyanka Sharan, Aidee Garcia‐Zintzun, Sandra Heckel, Purnesh Chattopadhyay, Linlin Wang, Martin Wittmann, Thomas Gemming, James Saenz, Juliane Simmchen
ChemNanoMat.2021; 7(9): 1042. CrossRef
Tuning Microbial Activity via Programmatic Alteration of Cell/Substrate Interfaces
Alexey V. Gulyuk, Dennis R. LaJeunesse, Ramon Collazo, Albena Ivanisevic
Advanced Materials.2021;[Epub] CrossRef
Topography and Expansion Patterns at the Biofilm-Agar Interface in Bacillus subtilis Biofilms
Sarah Gingichashvili, Osnat Feuerstein, Doron Steinberg
Microorganisms.2020; 9(1): 84. CrossRef
Applying the handicap principle to biofilms: condition‐dependent signalling inBacillus subtilismicrobial communities
Keith D. Harris, Ilana Kolodkin‐Gal
Environmental Microbiology.2019; 21(2): 531. CrossRef
STUDYING THE INTERNAL STRESS HETEROGENEITY OF THE GROWING BIOFILM BY THE MICROPILLAR DEFORMATION OF THE GROWING SUBSTRATE
XIAOLING WANG, ZHAOCAN WANG, XING SHEN, YUHAO KONG, HUI ZHAO, XIAOQIANG YAN
Journal of Mechanics in Medicine and Biology.2019; 19(06): 1950070. CrossRef

Identification of cyst wall proteins of the hypotrich ciliate Euplotes encysticus using a proteomics approach: Bangzheng Wang , Tao Niu , Muhammad Zeeshan Bhatti , Fenfen Chen , Lin Wu , Jiwu Chen; J. Microbiol. 2017;55(7):545-553. Published online June 30, 2017; DOI: https://doi.org/10.1007/s12275-017-6422-7

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Abstract

Euplotes encysticus is a species of Hypotrich ciliates, which form cyst wall by secreting the special substances on encounter of adverse environment. It has critical significance to study the component and mechanism underlying resting cyst, during resisting unfavorable conditions in dormancy induction. The present study was aimed to investigate the effects of cyst wall proteins of Euplotes encysticus by using biochemical
methods
. Therefore, protein extracts were separated by SDSPAGE, identified and analyzed by MALDI-TOF MS and Bioinformatics tools. We detected 42 cyst wall proteins, 26 were functional proteins and 16 proteins consist of unknown function; which is consistent with cyst wall specificity. These results partially revealed the components of resting cyst wall formed after the cells differentiation of Euplotes encysticus. In addition, our data suggested that the function of cyst wall proteins are more likely involved in the mechanical protection, signal transduction, material transport, protein degradation and energy metabolism to survival, with potentially importance implications in the molecular mechanism of eukaryocyte dormancy under stress condition.

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Decryption of the survival “black box”: gene family expansion promotes the encystment in ciliated protists
Didi Jin, Chao Li, Xiao Chen, Yurui Wang, Khaled A. S. Al-Rasheid, Naomi A. Stover, Chen Shao, Tengteng Zhang
BMC Genomics.2024;[Epub] CrossRef
Role of chitin synthases CHS1 and CHS2 in biosynthesis of the cyst wall of Cryptocaryon irritans
Huicheng Wu, Yihao Cen, Yipei Lu, Pengbo Dan, Yanwei Li, Xueming Dan, Zequan Mo
International Journal of Biological Macromolecules.2024; 280: 136143. CrossRef
Water temperature affects Cryptocaryon irritans development, cryptocaryoniasis occurrence and an auxiliary treatment decision-making webpage
Fei Yin, Jing Yin, Xiao Xie, Linhua Jiang
Aquaculture.2023; 574: 739694. CrossRef
A light and electron microscopical study on the resting cyst of the tintinnid Schmidingerella (Alveolata, Ciliophora) including a phylogeny-aware comparison
Maximilian H. Ganser, Heidi Bartel, Birgit Weißenbacher, Ancuela Andosch, Ursula Lütz-Meindl, Peter Radacher, Sabine Agatha
European Journal of Protistology.2022; 86: 125922. CrossRef
How Ciliated Protists Survive by Cysts: Some Key Points During Encystment and Excystment
Yuqing Li, Yurui Wang, Shijing Zhang, Xyrus X. Maurer-Alcalá, Ying Yan
Frontiers in Microbiology.2022;[Epub] CrossRef
Early signaling pathways mediating dormant cyst formation in terrestrial unicellular eukaryoteColpoda
Tatsuomi Matsuoka
FEMS Microbiology Letters.2021;[Epub] CrossRef
Novel insights into molecular mechanisms of Pseudourostyla cristata encystment using comparative transcriptomics
Nan Pan, Tao Niu, Muhammad Zeeshan Bhatti, Haiyang Zhang, Xinpeng Fan, Bing Ni, Jiwu Chen
Scientific Reports.2019;[Epub] CrossRef
New contribution to the morphology and molecular mechanism of Euplotes encysticus encystment
Fenfen Chen, Yanyan Xue, Nan Pan, Muhammad Zeeshan Bhatti, Tao Niu, Jiwu Chen
Scientific Reports.2018;[Epub] CrossRef
Morphological and Molecular Redefinition of Euplotes platystoma Dragesco & Dragesco‐Kernéis, 1986 and Aspidisca lynceus (Müller,1773) Ehrenberg, 1859, with Reconsideration of a “Well‐known” Euplotes Ciliate, Euplotes harpa Stein, 1859 (Ciliophora, Euploti
Chunyu Lian, Xiaotian Luo, Xinpeng Fan, Jie Huang, Yuhe Yu, William Bourland, Weibo Song
Journal of Eukaryotic Microbiology.2018; 65(4): 531. CrossRef
The affiliation of Hexasterias problematica and Halodinium verrucatum sp. nov. to ciliate cysts based on molecular phylogeny and cyst wall composition
Pieter R. Gurdebeke, Kenneth Neil Mertens, Yoshihito Takano, Aika Yamaguchi, Kara Bogus, Micah Dunthorn, Kazumi Matsuoka, Henk Vrielinck, Stephen Louwye
European Journal of Protistology.2018; 66: 115. CrossRef
Ultrastructure of vegetative cells and resting cysts, and live observations of the encystation and excystation processes in Diophrys oligothrix Borror, 1965 (Protista, Ciliophora)
Zhi‐Wei Gong, Xin‐Peng Fan, Rui Ma, Bing Ni
Journal of Morphology.2018; 279(10): 1397. CrossRef

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Effects of a Dark-Septate Endophytic Isolate LBF-2 on the Medicinal Plant Lycium barbarum L.: Hai-han Zhang , Ming Tang , Hui Chen , Ya-jun Wang; J. Microbiol. 2012;50(1):91-96. Published online February 27, 2012; DOI: https://doi.org/10.1007/s12275-012-1159-9

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Abstract: Dark septate endophytes (DSE) are ubiquitous root associated fungi; however, our understanding of their ecological function remains unclear. Here, we investigated the positive effect of a DSE fungus on its host plant Lycium barbarum L. A DSE isolate, LBF-2, isolated from the roots of L. barbarum, was inoculated onto the roots of plants, which were grown under greenhouse conditions for five weeks. The result of molecular analyses of internal transcribed spacer regions indicated that LBF-2 was 96% similar to Paraphoma chrysanthemicola. Melanized septate hyphae were observed in the root cortical cells of L. barbarum using a light microscope. Inoculation with LBF-2 increased the total biomass by 39.2% and also enhanced chlorophyll fluorescence. Inoculation increased the concentration of total chlorophyll by 22.8% and of chlorophyll a by 21.3%, relative to uninoculated controls. These data indicate that the LBF-2 isolate might be used to facilitate the cultivation of L. barbarum, which has medicinal applications.

Ecological Development and Genetic Diversity of Microcystis aeruginosa from Artificial Reservoir in Russia: Nikolay A. Gaevsky , Vladimir I. Kolmakov , Olga I. Belykh , Irina V. Tikhonova , Yochan Joung , Tae Seok Ahn , Valentina A. Nabatova , Anna S. Gladkikh; J. Microbiol. 2011;49(5):714-720. Published online November 9, 2011; DOI: https://doi.org/10.1007/s12275-011-0523-5

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14 Scopus

Abstract: Microcystis aeruginosa is a well-known Cyanobacterium responsible for the formation of toxic water blooms around the world. Shallow, warm, and eutrophic reservoirs provide the most favourable conditions for M. aeruginosa development. Numerous studies have been devoted to this species, but there still is a necessity to develop additional approaches for the monitoring of cyanobacteria in reservoirs. In this study, M. aeruginosa in the water column of a hypereutrophic Siberian reservoir was investigated by fluorescence, light, and electron microscopy as well as genetic analysis using a mcyE marker. Here, we demonstrate the genetic diversity and features of the fluorescence spectra for different ecotypes of this species. We suggest that a fluorescence approach can be used to identify M. aeruginosa in a natural environment in order to increase the effectiveness of ecological monitoring and water quality evaluation.

Isolation, Characterization, and Abundance of Filamentous Members of Caldilineae in Activated Sludge: Dae-No Yoon , Soo-Je Park , So-Jeong Kim , Che Ok Jeon , Jong-Chan Chae , Sung-Keun Rhee; J. Microbiol. 2010;48(3):275-283. Published online June 23, 2010; DOI: https://doi.org/10.1007/s12275-010-9366-8

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49 Scopus

Abstract: Chloroflexi are currently believed to serve as backbone forming agents in the activated sludge of wastewater treatment plants (WWTPs). In this study, we isolated and characterized filamentous bacteria in the class Caldilineae of the phylum Chloroflexi in municipal WWTPs. Diversity analysis using Chloroflexi-specific 16S rRNA gene clone libraries showed that 97% of the clones belonged to the subdivision Anaerolineae comprising the two classes Anaerolineae (95%) and Caldilineae (2%). Clones of Caldilineae were related to a thermophilic filament Caldilinea aerophila with 93% 16S rRNA gene sequence similarity. We obtained filamentous isolates classified into the class Caldilineae showing the best match to C. aerophila with 89% 16S rRNA gene sequence similarity. Isolates showed no ability to assimilate glucose or N-acetylglucosamine or to degrade biopolymers which were observed in filamentous Chloroflexi of WWTPs. The assessment of relative abundance based on quantitative PCR of the 16S rRNA gene indicated that members of the class Caldilineae comprised 12-19% of the Chloroflexi in the activated sludge. Additionally, fluorescence in situ hybridization experiments showed that diverse filamentous Caldilineae inhabit the activated sludge of municipal WWTPs. These findings yield insight into the role of filamentous mesophilic Caldilinea in stabilizing flocs of activated sludge in a wide range of WWTPs.

Journal Article

Monitoring of Algicidal Bacterium, Alteromonas sp. Strain A14 in its Application to Natural Cochlodinium polykrikoides Blooming Seawater Using Fluorescence In Situ Hybridization: Bo-Kyung Lee , Toshiya Katano , Shin-Ichi Kitamura , Myung-Joo Oh , Myung-Soo Han; J. Microbiol. 2008;46(3):274-282. Published online July 5, 2008; DOI: https://doi.org/10.1007/s12275-007-0238-9

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33 Scopus

Abstract: The red tide of dinoflagellate, Cochlodinium polykrikoides has frequently occurred in coastal waters, causing severe damage to fisheries. In the present study, the algicidal bacterium Alteromonas sp. A14 isolated from the southern coast of Korea was applied to a red tide of C. polykrikoides in a laboratory experiment. In the experiment, the abundance of the strain A14 was monitored using fluorescence in situ hybridization. Inoculation of the A14 at a final cell density of 9.0×105 cells/ml caused a significant decrease in C. polykrikoides abundance from 1,830 to 700 cells/ml during 2 days, while abundances of harmless diatoms rapidly increased from 3 days. Abundances of both A14 and other bacteria increased to 1 day. After 1 day, with flagellate abundance increased, bacterial abundance decreased. Finally, algicidal bacterial abundance decreased to 3.5×104 cells/ml. In the biological control of harmful algal blooms, in addition to decrease in target algal abundance and not occurrence of other harmful blooms, decrease in abundance of utilized organism is also important. This study emphasizes the importance of monitoring the inoculated bacterium when applying bacterium to natural seawater.

Production and Characterization of Monoclonal Antibodies to Human Immunodeficiency Virus Type 1 gp120 Envelope Glycoprotein: Choi, Eui Yul , Ryu, Ji Yoon , Lee, Yoon , Ha, Sung Gil , Chung, So Young , Park, Sang Yeol , Nham, Sang Uk , Lee, Young Ik , Park, Jin Seu; J. Microbiol. 1998;36(1):59-65.

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Abstract: Monoclonal antibodies to human immunodeficiency virus type 1 envelope glycoprotein gp 120(HIV-1 gp120) were produced and characterized. For immunogen recombinant gp120 polypeptide expressed in bacteria was prepared and injected into mice. From two fusion experiments, twenty hybridomas secreting monoclonal antibodies against the recombinant gp120 were initially screened by immunodot blot analysis. Among the antibodies, 15 of them showed strong reactivities with the recombinant protein expressed in bacteria in Western blot and thus it was tested if these could react with the recombinant protein expressed in insect cells. All of the 15 antibodies immunostained the protein band with varing degrees of reactivities. Next, we tested whether the antibodies recognize authentic gp120 protein expressed in mammalian cells. COS-1 cells were tranfected with the cDNA encoding gp120 protein, and the transiently ecpressed protein were analyzed with the mAbs by Western blot analysis and immunofluorescence microscopy. Six of the monoclonal antibodies reacted with the protein band of authentic gp120 expressed in mammalian cells in the Western blot, and five stained the cell periphery of the transfected COS-1 cells in immunofluorescence. The mAbs described in this study should prove to be useful tools for the biochemical, immunological and structural analysis of HIV-1 gp120 envelope glycoprotein.

Expression of Human Cytomegalovirus Immediate Early US3 Gene in Human Fibroblast Cells: Gyu-Cheol Lee , Chong-Kyo Lee , Jin-Hyun Ahn , Chan-Hee Lee; J. Microbiol. 2000;38(1):24-30.

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Abstract: US3 gene is a member of the human cytomegalovirus (HCMV) immediate early gene. Although the precise functions of the US3 gene in HCMV replication and pathogenesis are not known, it has been reported to play a role in inhibiting major histocompatibility class I antigen presentation. For further knowledge of US3 gene expression, rabbit polyclonal antiserum of the US3 gene product was used for indirect immunofluorescence assay. In permissive human foreskin fibroblast (HFF) cells, US3 gene expression was detectable as crescent or half-moon shape in the perinuclear region at immediate early times after virus infection. HFF cells infected with mutant HCMV lacking US3 open reading frames were negative for US3 immunofluorescence assay. Double immunofluorescence assay using monoclonal antibody to gamma adaptin (specific for the Golgi complex) and rabbit anti-US3 antiserum revealed that US3 gene product could be localized to the Golgi complex. At later time after HCMV infection, US3 gene products were detected as globular aggregates in the cytosol. These aggregates were positive for gamma adaptin and stained with preimmune serum, suggesting a nonspecific reaction to the Golgi complex. Northern blot analysis revealed that transcription of US3 was observed only during immediate early times after virus infection (until 6 h postinfection). Therefore US3 gene expression appears to be confined to immediate early time and its gene products are localized to the Golgi complex as cres-cent shaped forms in the perinuclear cytoplasm.

Fluorescence Microscopy of Condensed DNA Conformations of Bacterial Cells: Erhan Suleymanoglu; J. Microbiol. 2002;40(4):319-326.

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Abstract: Cellular DNA in prokaryotes is organized in nucleic acid-protein self-assemblies referred to as the nucleoid. The physical forces responsible for its stability inside the poor solvent properties of the cytoplasm and their functional implications are not understood. Studies on the organisation and functioning of the cytosol of cells largely rely on experimental protocols performed in highly dilute solutions using biochemically purified molecules, which is not a reliable substitute for the situation existing in vivo. Our current research interest is focused on the characterization of biological and physical forces determining the compaction and phase separation of DNA in Escherichia coli cytoplasm. We have emphasized the effect of excluded volume in solutions with high macromolecular concentrations (macromolecular crowding) upon self-association patterns of reactions. The prokaryotic cytosol was simulated by addition of inert polymer polyethylene glycol (PEG) (average molecular weight 20000), as an agent which afterwards facilitates the self-association of macromolecules. Fluorescence microscopy was used for direct visualization of nucleoids in intact cells, after staining with DAPI (4',6-diamidino-2-phenylindole dihydrochloride). Addition of the crowding agent PEG 20,000, in increasing concentrations generated progressively enhanced nucleoid compaction, the effect being stronger in the presence of 0.2 M NaCl and 5 uM MgCl_2. Under these conditions, the nucleoids were compacted to volumes of around 2㎛^3 or comparable sizes with that of living cells.

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