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Research Support, Non-U.S. Gov't
- Degradation of Endocrine Disrupting Chemicals by Genetic Transformants with Two Lignin Degrading Enzymes in Phlebia tremellosa
- Hyunwoo Kum , Sungsuk Lee , Sunhwa Ryu , Hyoung T. Choi
- J. Microbiol. 2011;49(5):824-827. Published online November 9, 2011
- DOI: https://doi.org/10.1007/s12275-011-1230-y
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- 7 Crossref
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Abstract
PDF - A white rot fungus Phlebia tremellosa produced lignin degrading enzymes, which showed degrading activity against various recalcitrant compounds. However, manganese peroxidase (MnP) activity, one of lignin degrading enzymes, was very low in this fungus under various culture conditions. An expression vector that carried both the laccase and MnP genes was constructed using laccase genomic DNA of P. tremellosa and MnP cDNA from Polyporus brumalis. P. tremellosa was genetically transformed using the expression vector to obtain fungal transformants showing increased laccase and MnP activity. Many transformants showed highly increased laccase and MnP activity at the same time in liquid medium, and three of them were used to degrade endocrine disrupting chemicals. The transformant not only degraded bisphenol A and nonylphenol more rapidly but also removed the estrogenic activities of the chemicals faster than the wild type strain.
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Citations
Citations to this article as recorded by
- Fungi for the bioremediation of pharmaceutical-derived pollutants: A bioengineering approach to water treatment
Galit Akerman-Sanchez, Keilor Rojas-Jimenez
Environmental Advances.2021; 4: 100071. CrossRef - Complete degradation of bisphenol A and nonylphenol by a composite of biogenic manganese oxides and Escherichia coli cells with surface-displayed multicopper oxidase CotA
Zhen Zhang, Zhiyong Ruan, Jin Liu, Chang Liu, Fuming Zhang, Robert J. Linhardt, Lin Li
Chemical Engineering Journal.2019; 362: 897. CrossRef - Laccase production and pellet morphology of Coprinopsis cinerea transformants in liquid shake flask cultures
Martin Rühl, Karin Lange, Ursula Kües
Applied Microbiology and Biotechnology.2018; 102(18): 7849. CrossRef - Fate of Bisphenol A in Terrestrial and Aquatic Environments
Jeongdae Im, Frank E. Löffler
Environmental Science & Technology.2016; 50(16): 8403. CrossRef - Molecular characterization of manganese peroxidases from white-rot fungus Polyporus brumalis
Sun-Hwa Ryu, Boyeong Kim, Myungkil Kim, Jin-Ho Seo
Bioprocess and Biosystems Engineering.2014; 37(3): 393. CrossRef - Protoplast Isolation and Genetic Transformation of Polyporus brumalis
Sun-Hwa Ryu, Myung-Kil Kim
The Korean Journal of Microbiology.2014; 50(4): 372. CrossRef - Enhanced Lignin Biodegradation by a Laccase-Overexpressed White-Rot Fungus Polyporus brumalis in the Pretreatment of Wood Chips
Sun-Hwa Ryu, Myung-Kil Cho, Myungkil Kim, Sang-Min Jung, Jin-Ho Seo
Applied Biochemistry and Biotechnology.2013; 171(6): 1525. CrossRef
- Fungi for the bioremediation of pharmaceutical-derived pollutants: A bioengineering approach to water treatment
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