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- Direct current exerts electricidal and bioelectric effects on Porphyromonas gingivalis biofilms partially via promoting oxidative stress and antibiotic transport
- Peihui Zou , Peng Li , Jia Liu , Pei Cao , Qingxian Luan
- J. Microbiol. 2022;60(1):70-78. Published online November 26, 2021
- DOI: https://doi.org/10.1007/s12275-022-1238-5
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- 7 Web of Science
- 7 Crossref
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Abstract
- Low electric current can inhibit certain microbial biofilms and enhance the efficacy of antimicrobials against them. This study investigated the electricidal and bioelectric effects of direct current (DC) against Porphyromonas gingivalis biofilms as well as the underlying mechanisms. Here, we firstly showed that DC significantly suppressed biofilm formation of P. gingivalis in time- and intensity-dependent manners, and markedly inhibited preformed P. gingivalis biofilms. Moreover, DC enhanced the killing efficacy of metronidazole (MTZ) and amoxicillin with clavulanate potassium (AMC) against the biofilms. Notably, DC-treated biofilms displayed upregulated intracellular ROS and expression of ROS related genes (sod, feoB, and oxyR) as well as porin gene. Interestingly, DC-induced killing of biofilms was partially reversed by ROS scavenger N-dimethylthiourea (DMTU), and the synergistic effect of DC with MTZ/AMC was weakened by small interfering RNA of porin gene (si-Porin). In conclusion, DC can exert electricidal and bioelectric effects against P. gingivalis biofilms partially via promotion of oxidative stress and antibiotic transport, which offers a promising approach for effective management of periodontitis.
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- Antifungal Activity, Synergism with Fluconazole or Amphotericin B and Potential Mechanism of Direct Current against Candida albicans Biofilms and Persisters
Peihui Zou, Jia Liu, Peng Li, Qingxian Luan
Antibiotics.2024; 13(6): 521. CrossRef - Smart dental materials for antimicrobial applications
Carolina Montoya, Lina Roldan, Michelle Yu, Sara Valliani, Christina Ta, Maobin Yang, Santiago Orrego
Bioactive Materials.2023; 24: 1. CrossRef - Molecular Study of Porphyromonas Gingivalis Strains With fimA Genotypes in Periodontitis Patients
Zhraa F. Faruq, Sami Khalaf Jabar
Iranian Journal of Medical Microbiology.2023; 17(6): 663. CrossRef - Drug delivery approaches for enhanced antibiofilm therapy
Tao Wang, Erik Jan Cornel, Chang Li, Jianzhong Du
Journal of Controlled Release.2023; 353: 350. CrossRef - Weak direct current exerts synergistic effect with antibiotics and reduces the antibiotic resistance: An in vitro subgingival plaque biofilm model
Peihui Zou, Yanfeng Wang, Pei Cao, Peng Li, Jia Liu, Qingxian Luan
Journal of Periodontal Research.2023; 58(1): 143. CrossRef - Bioelectric device for effective biofilm inflammation management of dental implants
Jihyun Lee, Young Wook Kim
Scientific Reports.2023;[Epub] CrossRef - A Highly Efficacious Electrical Biofilm Treatment System for Combating Chronic Wound Bacterial Infections
Fan Zhao, Yajuan Su, Junying Wang, Svetlana Romanova, Dominick J. DiMaio, Jingwei Xie, Siwei Zhao
Advanced Materials.2023;[Epub] CrossRef
- Antifungal Activity, Synergism with Fluconazole or Amphotericin B and Potential Mechanism of Direct Current against Candida albicans Biofilms and Persisters
Research Support, Non-U.S. Gov't
- Isolation and Analyses of Uranium Tolerant Serratia marcescens Strains and Their Utilization for Aerobic Uranium U(VI) Bioadsorption
- Rakshak Kumar , Celin Acharya , Santa Ram Joshi
- J. Microbiol. 2011;49(4):568-574. Published online September 2, 2011
- DOI: https://doi.org/10.1007/s12275-011-0366-0
- 47 View
- 0 Download
- 52 Scopus
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Abstract
- Enrichment-based methods targeted at uranium-tolerant populations among the culturable, aerobic, chemoheterotrophic bacteria from the subsurface soils of Domiasiat (India’s largest sandstone-type uranium deposits, containing an average ore grade of 0.1% U3O8), indicated a wide occurrence of Serratia marcescens. Five representative S. marcescens isolates were characterized by a polyphasic taxonomic approach. The phylogenetic analyses of 16S rRNA gene sequences showed their relatedness to S. marcescens ATCC 13880 (≥99.4% similarity). Biochemical characteristics and random amplified polymorphic DNA profiles revealed significant differences among the representative isolates and the type strain as well. The minimum inhibitory concentration for uranium U(VI) exhibited by these natural isolates was found to range from 3.5-4.0 mM. On evaluation for their uranyl adsorption properties, it was found that all these isolates were able to remove nearly 90-92% (21-22 mg/L) and 60-70% (285-335 mg/L) of U(VI) on being challenged with 100 μM (23.8 mg/L) and 2 mM (476 mg/L) uranyl nitrate solutions, respectively, at pH 3.5 within 10 min of exposure. his capacity was retained by the isolates even after 24 h of incubation. Viability tests confirmed the tolerance of these isolates to toxic concentrations of soluble uranium U(VI) at pH 3.5. This is among the first studies to report uranium-tolerant aerobic chemoheterotrophs obtained from the pristine uranium ore-bearing site of Domiasiat.
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