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- Kaposi’s Sarcoma-Associated Herpesvirus Viral Protein Kinase Interacts with RNA Helicase A and Regulates Host Gene Expression
- Jae Eun Jong , Junsoo Park , Sunmi Kim , Taegun Seo
- J. Microbiol. 2010;48(2):206-212. Published online May 1, 2010
- DOI: https://doi.org/10.1007/s12275-010-0021-1
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- 9 Crossref
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Abstract
PDF - RNA helicase A (RHA) containing the DExH motif is a human homolog of maleless protein that regulates expression of genes located in the Drosophila X chromosome during dosage compensation. RHA exerts helicase activity that unwinds double-stranded RNA and DNA to a single-strand form. The protein acts as a bridging factor mediating interactions of CBP/p300 and RNA pol II, and consequently affects gene expression. Kaposi’s sarcoma-associated herpesvirus (KSHV) is a member of the γ-herpesvirus subfamily that causes several disorders. The majority of herpesviruses commonly encode predicted viral protein kinases. KSHV open reading frame 36 (ORF36) codes for protein kinase domains, and functions as a serine/threonine protein kinase. KSHV ORF36 is classified as a late gene, as it is expressed during lytic replication and localized in the nuclei of KSHV-infected cells. Recent studies show that viral protein kinase (vPK) interacts with cellular proteins. In this study, we determined the cellular localization of vPK in KSHVinfected BCBL-1 cells using confocal microscopy. Proteomic analysis indicates that cellular proteins interacted with vPK, and co-immunoprecipitation reactions further reveal interactions between vPK and RHA. Moreover, KSHV vPK appeared to regulate the transcriptional activation of Cre promoter, and plays an important role in cellular transcription of RHA.
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Citations
Citations to this article as recorded by
- Population Genetic Structure and Human Adaptation of Kaposi Sarcoma–Associated Herpesvirus
Alessandra Mozzi, Diego Forni, Rachele Cagliani, Cristian Molteni, Mario Clerici, Manuela Sironi
Open Forum Infectious Diseases.2025;[Epub] CrossRef - RNA helicase A as co-factor for DNA viruses during replication
Fan Guo, Li Xing
Virus Research.2021; 291: 198206. CrossRef - Mechanism of Angiopoietin-1 Upregulation in Kaposi's Sarcoma-Associated Herpesvirus-Infected PEL Cell Lines
Xin Zheng, Eriko Ohsaki, Keiji Ueda, R. M. Longnecker
Journal of Virology.2015; 89(9): 4786. CrossRef - The Expanding Functions of Cellular Helicases: The Tombusvirus RNA Replication Enhancer Co-opts the Plant eIF4AIII-Like AtRH2 and the DDX5-Like AtRH5 DEAD-Box RNA Helicases to Promote Viral Asymmetric RNA Replication
Nikolay Kovalev, Peter D. Nagy, Lee Gehrke
PLoS Pathogens.2014; 10(4): e1004051. CrossRef - A Co-Opted DEAD-Box RNA Helicase Enhances Tombusvirus Plus-Strand Synthesis
Nikolay Kovalev, Judit Pogany, Peter D. Nagy, Shou-Wei Ding
PLoS Pathogens.2012; 8(2): e1002537. CrossRef - Application of Proteomics to Soft Tissue Sarcomas
Tadashi Kondo, Daisuke Kubota, Akira Kawai
International Journal of Proteomics.2012; 2012: 1. CrossRef - Similar roles for yeast Dbp2 and Arabidopsis RH20 DEAD-box RNA helicases to Ded1 helicase in tombusvirus plus-strand synthesis
Nikolay Kovalev, Daniel Barajas, Peter D. Nagy
Virology.2012; 432(2): 470. CrossRef - The function and architecture of DEAH/RHA helicases
Yangzi He, Gregers R. Andersen, Klaus H. Nielsen
BioMolecular Concepts.2011; 2(4): 315. CrossRef - Recognition of herpesviruses by the innate immune system
Søren R. Paludan, Andrew G. Bowie, Kristy A. Horan, Katherine A. Fitzgerald
Nature Reviews Immunology.2011; 11(2): 143. CrossRef
- Population Genetic Structure and Human Adaptation of Kaposi Sarcoma–Associated Herpesvirus
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