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Molecular analysis of the interaction between ubiquitin-specific protease 7 and large T antigen of Merkel cell polyomavirus
Dahwan Lim, Jung-Hwan Park, Ho-Chul Shin, Seung Jun Kim, Bonsu Ku
Received November 10, 2025  Accepted December 23, 2025  Published online February 12, 2026  
DOI: https://doi.org/10.71150/jm.2511009    [Epub ahead of print]
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  • 4 Download
AbstractAbstract PDFSupplementary Material

Merkel cell polyomavirus (MCPyV) is the primary causative agent of Merkel cell carcinoma, a rare but highly aggressive neuroendocrine skin cancer. Large T antigen (LT), one of two oncoproteins encoded by MCPyV, sustains the proliferation of MCPyV-infected tumor cells. LT contains multiple protein-binding motifs that mediate interactions with diverse host proteins essential for its function. Among these, ubiquitin-specific protease 7 (Usp7), a deubiquitinase that regulates the stability of multiple substrates, including p53, is a recently identified LT-interacting protein. In the present study, we characterized the intermolecular interaction between Usp7 and MCPyV LT using biochemical analyses and AlphaFold-based structural modeling. Our results demonstrate that MCPyV LT directly interacts with the TRAF domain of Usp7 via a unique binding motif that is distinct from the canonical sequence. Moreover, MCPyV LT attenuates the p53-deubiquitinating activity of Usp7, providing insights into the molecular function of this viral oncoprotein.
Multi-omics to evaluate the protective mechanisms during Akkermansia muciniphila treatment of Candida albicans colonization and subsequent infection
Qiulin Luo, Huan Zhang, Youming Pu, Yingpu Wei, Jiangkun Yu, Xiaoshen Wang, Qin Cai, Ying Hu, Wenli Yuan
J. Microbiol. 2025;63(8):e2502007.   Published online August 31, 2025
DOI: https://doi.org/10.71150/jm.2502007
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AbstractAbstract PDFSupplementary Material

Akkermansia muciniphila (AKK, A. muciniphila) fortifies the intestinal barrier, inhibits the colonization of pathogenic bacteria, and protects the host’s health. Nevertheless, the existing literature offers inadequate evidence to ascertain whether A. muciniphila can effectively treat Candida albicans (C. albicans) infections in vitro, and the underlying mechanisms remain ambiguous. This study, animal models were established through gavage with clinical isolates of C. albicans to induce gastrointestinal tract colonization and subsequent translocation infection. The models were subsequently administered A. muciniphila. We examined the analysis of 16S rRNA gene sequencing, metabolomics of colonic contents, and transcriptomics of colonic tissue. The intestinal barrier, inflammatory responses, and immune cell infiltration are analyzed. This study revealed that A. muciniphila markedly mitigated C. albicans translocation infection and modified the intestinal microbial community structure and metabolic attributes in model mice. After administering A. muciniphila to the translocation infection group, there was a notable increase in the prevalence of bacteria that produce short-chain fatty acids, including Eubacterium_F. Moreover, there was a significant increase in the levels of specific pathogens, including Faecalibaculum, Turicibacter, and Turicimonas. The study demonstrated that A. muciniphila treatment can improve the composition of intestinal microbiota and metabolites, augment the tight junctions of colonic tissue and diminish systemic inflammatory response. This presents an innovative therapeutic approach for the potential treatment of intestinal C. albicans infection using A. muciniphila.

Characterization of novel bacteriophages for effective phage therapy against Vibrio infections in aquaculture
Kira Moon, Sangdon Ryu, Seung Hui Song, Se Won Chun, Nakyeong Lee, Aslan Hwanhwi Lee
J. Microbiol. 2025;63(5):e2502009.   Published online May 27, 2025
DOI: https://doi.org/10.71150/jm.2502009
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  • 2 Web of Science
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AbstractAbstract PDFSupplementary Material

The widespread use of antibiotics in aquaculture has led to the emergence of multidrug-resistant pathogens and environmental concerns, highlighting the need for sustainable, eco-friendly alternatives. In this study, we isolated and characterized three novel bacteriophages from aquaculture effluents in Korean shrimp farms that target the key Vibrio pathogens, Vibrio harveyi, and Vibrio parahaemolyticus. Bacteriophages were isolated through environmental enrichment and serial purification using double-layer agar assays. Transmission electron microscopy revealed that the phages infecting V. harveyi, designated as vB_VhaS-MS01 and vB_VhaS-MS03, exhibited typical Siphoviridae morphology with long contractile tails and icosahedral heads, whereas the phage isolated from V. parahaemolyticus (vB_VpaP-MS02) displayed Podoviridae characteristics with an icosahedral head and short tail.

Whole-genome sequencing produced complete, circularized genomes of 81,710 bp for vB_VhaS-MS01, 81,874 bp for vB_VhaS-MS03, and 76,865 bp for vB_VpaP-MS02, each showing a modular genome organization typical of Caudoviricetes. Genomic and phylogenetic analyses based on the terminase large subunit gene revealed that although vB_VhaS-MS01 and vB_VhaS-MS03 were closely related, vB_VpaP-MS02 exhibited a distinct genomic architecture that reflects its unique morphology and host specificity. Collectively, these comparative analyses demonstrated that all three phages possess genetic sequences markedly different from those of previously reported bacteriophages, thereby establishing their novelty. One-step growth and multiplicity of infection (MOI) experiments demonstrated significant differences in replication kinetics, such as burst size and lytic efficiency, among the phages, with vB_VhaS-MS03 maintaining the most effective bacterial control, even at an MOI of 0.01. Additionally, host range assays showed that vB_VhaS-MS03 possessed a broader spectrum of activity, supporting its potential use as a stand-alone agent or key component of phage cocktails. These findings highlight the potential of region-specific phage therapy as a targeted and sustainable alternative to antibiotics for controlling Vibrio infections in aquaculture.

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  • Revolutionizing seafood safety with bacteriophages: emerging technologies and applications
    Nigar Sultana Meghla, Soo-Jin Jung, Md Furkanur Rahaman Mizan, Syeda Roufun Nesa, IkSoon Kang, Sang-Do Ha
    Food Microbiology.2026; 137: 105021.     CrossRef
  • Genomic characterization of APEC phages and evaluation of the efficacy in reducing the loads of APEC O78 infections in chickens
    Qin Lu, Xinxin Jin, Zui Wang, Rongrong Zhang, Yunqing Guo, Qiao Hu, Wenting Zhang, Tengfei Zhang, Qingping Luo
    Frontiers in Microbiology.2026;[Epub]     CrossRef
  • Feed Additives in Aquaculture: Benefits, Risks, and the Need for Robust Regulatory Frameworks
    Ekemini Okon, Matthew Iyobhebhe, Paul Olatunji, Mary Adeleke, Nelson Matekwe, Reuben Okocha
    Fishes.2025; 10(9): 471.     CrossRef
Genomic profiling of soil nitrifying microorganisms enriched on floating membrane filter
Christiana Abiola, Joo-Han Gwak, Ui-Ju Lee, Aderonke Odunayo Adigun, Sung-Keun Rhee
J. Microbiol. 2025;63(4):e2502002.   Published online April 29, 2025
DOI: https://doi.org/10.71150/jm.2502002
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AbstractAbstract PDFSupplementary Material

Recently, floating membrane filter cultivation was adopted to simulate solid surface and enrich surface-adapted soil ammonia-oxidizing archaea (AOA) communities from agricultural soil, as opposed to the conventional liquid medium. Here, we conducted metagenomic sequencing to recover nitrifier bins from the floating membrane filter cultures and reveal their genomic properties. Phylogenomic analysis showed that AOA bins recovered from this study, designated FF_bin01 and FF_bin02, are affiliated with the Nitrososphaeraceae family, while the third bin, FF_bin03, is a nitrite-oxidizing bacterium affiliated with the Nitrospiraceae family. Based on the ANI/AAI analysis, FF_bin01 and FF_bin02 are identified as novel species within the genera “Candidatus Nitrosocosmicus” and Nitrososphaera, respectively, while FF_bin03 represents a novel species within the genus Nitrospira. The pan and core genome analysis for the 29 AOA genomes considered in this study revealed 5,784 orthologous clusters, out of which 653 were core orthologous clusters. Additionally, 90 unique orthologous clusters were conserved among the Nitrososphaeraceae family, suggesting their potential role in enhancing culturability and adaptation to diverse environmental conditions. Intriguingly, FF_bin01 and FF_bin02 harbor a gene encoding manganese catalase and FF_bin03 also possesses a heme catalase gene, which might enhance their growth on the floating membrane filter. Overall, the floating membrane filter cultivation has proven to be a promising approach for isolating distinct soil AOA, and further modifications to this technique could stimulate the growth of a broader range of uncultivated nitrifiers from diverse soil environments.
Whole-genome characterization and global phylogenetic comparison of cefotaxime-resistant Escherichia coli isolated from broiler chickens
Shahana Ahmed, Tridip Das, Chandan Nath, Tahia Ahmed, Keya Ghosh, Pangkaj Kumar Dhar, Ana Herrero-Fresno, Himel Barua, Paritosh Kumar Biswas, Md Zohorul Islam, John Elmerdahl Olsen
J. Microbiol. 2025;63(4):e2412009.   Published online April 29, 2025
DOI: https://doi.org/10.71150/jm.2412009
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AbstractAbstract PDFSupplementary Material

Antimicrobial resistance (AMR) poses a serious threat to public health, with the emergence of extended-spectrum beta-lactamases (ESBLs) in Enterobacteriaceae, particularly Escherichia coli, raising significant concerns. This study aims to elucidate the drivers of antimicrobial resistance, and the global spread of cefotaxime-resistant E. coli (CREC) strains. Whole-genome sequencing (WGS) was performed to explore genome-level characteristics, and phylogenetic analysis was conducted to compare twenty CREC strains from this study, which were isolated from broiler chicken farms in Bangladesh, with a global collection (n = 456) of CREC strains from multiple countries and hosts. The MIC analysis showed over 70% of strains isolated from broiler chickens exhibiting MIC values ≥ 256 mg/L for cefotaxime. Notably, 85% of the studied farms (17/20) tested positive for CREC by the end of the production cycle, with CREC counts increasing from 0.83 ± 1.75 log10 CFU/g feces on day 1 to 5.24 ± 0.72 log10 CFU/g feces by day 28. WGS revealed the presence of multiple resistance genes, including blaCTX-M, which was found in 30% of the strains. Phylogenetic comparison showed that the Bangladeshi strains were closely related to strains from diverse geographical regions and host species. This study provides a comprehensive understanding of the molecular epidemiology of CREC. The close phylogenetic relationships between Bangladeshi and global strains demonstrate the widespread presence of cefotaxime-resistant bacteria and emphasize the importance of monitoring AMR in food-producing animals to mitigate the spread of resistant strains.

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  • ESBL-Producing E. coli in Captive Black Bears: Molecular Characteristics and Risk of Dissemination
    Xin Lei, Mengjie Che, Yuxin Zhou, Shulei Pan, Xue Yang, Siyu Liu, Iram Laghari, Mingyue Wu, Ruilin Han, Xiaoqi Li, Lei Zhou, Guangneng Peng, Haifeng Liu, Ziyao Zhou, Kun Zhang, Zhijun Zhong
    Veterinary Sciences.2025; 12(11): 1085.     CrossRef
Research Articles
Dissimilatory nitrate reductions in soil Neobacillus and Bacillus strains under aerobic condition
Seohyun Ahn, Min Cho, Michael J. Sadowsky, Jeonghwan Jang
J. Microbiol. 2025;63(2):e2411019.   Published online February 27, 2025
DOI: https://doi.org/10.71150/jm.2411019
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  • 4 Web of Science
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AbstractAbstract PDFSupplementary Material

Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) were thought to be carried-out by anaerobic bacteria constrained to anoxic conditions as they use nitrate (NO3-) as a terminal electron acceptor instead of molecular O2. Three soil bacilli, Neobacillus spp. strains PS2-9 and PS3-12 and Bacillus salipaludis PS3-36, were isolated from rice paddy field soil in Korea. The bacterial strains were selected as possible candidates performing aerobic denitrification and DNRA as they were observed to reduce NO3- and produce extracellular NH4+ regardless of oxygen presence at the initial screening. Whole genome sequencing revealed that these strains possessed all the denitrification and DNRA functional genes in their genomes, including the nirK, nosZ, nirB, and nrfA genes, which were simultaneously cotranscribed under aerobic condition. The ratio between the assimilatory and dissimilatory NO3- reduction pathways depended on the availability of a nitrogen source for cell growth, other than NO3-. Based on the phenotypic and transcriptional analyses of the NO3- reductions, all three of the facultative anaerobic strains reduced NO3- likely in both assimilatory and dissimilatory pathways under both aerobic and anoxic conditions. To our knowledge, this is the first report that describes coexistence of NO3- assimilation, denitrification, and DNRA in a Bacillus or Neobacillus strain under aerobic condition. These strains may play a pivotal role in the soil nitrogen cycle.

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  • Biofertilizers Enhance Soil Fertility and Crop Yields Through Microbial Community Modulation
    Xu Zhang, Lei Zhang, Junjie Liu, Zongzuan Shen, Zhuxiu Liu, Haidong Gu, Xiaojing Hu, Zhenhua Yu, Yansheng Li, Jian Jin, Guanghua Wang
    Agronomy.2025; 15(7): 1572.     CrossRef
  • Strategy of nitrate-enhanced natural attenuation for remediation of PAHs-contaminated subsoil
    Xuyang Jiang, Zhen Mao, Zhenqi Hu, Tao Jin, Licun Zhong, Jinbiao Yu
    Journal of Environmental Chemical Engineering.2025; 13(5): 118037.     CrossRef
  • Leveraging iron-rich recovered waste as a co-electron donor in sulfur autotrophic denitrification for simultaneous nitrate and phosphate removal from low C/N hydroponic wastewater
    Sandesh Pandey, Anup Gurung, Choe Earn Choong, Suleman Shahzad, Fida Hussain, Woochang Kang, Syed Ejaz Hussain Mehdi, Aparna Sharma, Min Jang, Sang-Eun Oh
    Journal of Water Process Engineering.2025; 79: 108948.     CrossRef
  • narG, rather than napA, mediates aerobic nitrate reduction process in Pseudomonas putida Y-9
    Yuwen Luo, Luo Luo, Xuejiao Huang, Daihua Jiang, Zhenlun Li
    Water Research X.2025; 29: 100437.     CrossRef
Small molecule kinase inhibitor altiratinib inhibits brain cyst forming bradyzoites of Toxoplasma gondii
Yeong Hoon Kim, Hye-Jin Ahn, Hwa Sun Kim, Ho-Woo Nam
J. Microbiol. 2025;63(2):e2409001.   Published online February 27, 2025
DOI: https://doi.org/10.71150/jm.2409001
  • 1,721 View
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AbstractAbstract PDFSupplementary Material

Chronic toxoplasmosis is caused by Toxoplasma gondii bradyzoites. This study assessed six candidate small molecule kinase inhibitors (SMKIs) against bradyzoites (ME49 strain), the reactivated form of the parasite resulting from the rupture of brain cysts. Bradyzoites were obtained from mouse brain cysts, cultured in ARPE-19 cells, and treated with afatinib and neratinib (HER2/HER4 inhibitors), ACTB-1003 and regorafenib (VEGFR-2 inhibitors), or altiratinib and foretinib (c-MET inhibitors). The effects on the growth of T. gondii were analyzed by western blot and immunofluorescence assay. Changes in the host cells were assessed using markers for cell viability, apoptosis, necrosis, and autophagy. All inhibitors blocked the growth of bradyzoites, although afatinib was less effective. Afatinib enhanced autophagy signals, while ACTB-1003 and neratinib affected mitochondrial biosynthesis and mitophagy. Altiratinib demonstrated an effect against bradyzoites at the lowest concentration with minimal impact on the host cells. It may be effective in blocking the reactivation of brain cysts in immunodeficiency patients caused by bradyzoites.
Lactic acid bacteria from Ethiopian traditional beverage, Tella: technological and metabolic profiles for industrial application
Gashaw Assefa Yehuala, Jaein Choe, Nurelegne Tefera Shibeshi, Kumsa Delessa, Asnake Desalegn, Mi-Kyung Park
J. Microbiol. 2025;63(1):e.2409008.   Published online December 20, 2024
DOI: https://doi.org/10.71150/jm.2409008
  • 2,300 View
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AbstractAbstract PDF

Tella is a traditional beverage widely accepted by consumers, despite the lack of product consistency owing to its reliance on natural fermentation. This study aimed to identify potential industrial lactic acid bacteria (LAB) starter cultures based on their technological properties. Seven LAB strains isolated from Tella were characterized for their carbohydrate utilization, salt content, temperature, and acid tolerances, growth and acidification rates, and metabolite profiles. Most strains efficiently utilized various carbohydrates, with Lactiplantibacillus plantarum TDM41 showing exceptional versatility. The strains exhibited similar growth characteristics. Principal component analysis of stress tolerance properties revealed that L. plantarum TDM41, Pediococcus pentosaceus TAA01, and Leuconostoc mesenteroides TDB22 exhibited superior tolerance ability. Strong acidification properties were detected in the L. plantarum TDM41, P. pentosaceus TAA01, and Leuconostoc mesenteroides TDB22 strains after 24 h incubation at 30°C. L. plantarum TDM41 displayed the fastest acidification rate throughout the analysis period. All LAB strains produced significant amounts of diverse organic acids, including lactic acid, citric acid, acetic acid, malic acid, and succinic acid, with lactic acid being the primary acid produced by each strain. Overall, strains L. plantarum TDM41 and P. pentosaceus TAA01 prove to be potential candidates for Tella industrial starter cultures and similar cereal products owing to their robust technological properties.

Citations

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  • Preparation method and physicochemical characteristics of Tella: an Ethiopian fermented beverage
    Rabira Lemessa Gudeta, Solomon Abera, Hirpha Adugna Areti
    Journal of Ethnic Foods.2025;[Epub]     CrossRef
Journal Articles
Rhodobacteraceae are Prevalent and Ecologically Crucial Bacterial Members in Marine Biofloc Aquaculture
Meora Rajeev, Jang-Cheon Cho
J. Microbiol. 2024;62(11):985-997.   Published online November 15, 2024
DOI: https://doi.org/10.1007/s12275-024-00187-0
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  • 12 Crossref
AbstractAbstract PDF
Bioflocs are microbial aggregates primarily composed of heterotrophic bacteria that play essential ecological roles in maintaining animal health, gut microbiota, and water quality in biofloc aquaculture systems. Despite the global adoption of biofloc aquaculture for shrimp and fish cultivation, our understanding of biofloc microbiota-particularly the dominant bacterial members and their ecological functions-remains limited. In this study, we employed integrated metataxonomic and metagenomic approaches to demonstrate that the family Rhodobacteraceae of Alphaproteobacteria consistently dominates the biofloc microbiota and plays essential ecological roles. We first analyzed a comprehensive metataxonomic dataset consisting of 200 16S rRNA gene amplicons collected across three Asian countries: South Korea, China, and Vietnam. Taxonomic investigation identified Rhodobacteraceae as the dominant and consistent bacterial members across the datasets. The predominance of this taxon was further validated through metagenomics approaches, including read taxonomy and read recruitment analyses. To explore the ecological roles of Rhodobacteraceae, we applied genome-centric metagenomics, reconstructing 45 metagenome-assembled genomes. Functional annotation of these genomes revealed that dominant Rhodobacteraceae genera, such as Marivita, Ruegeria, Dinoroseobacter, and Aliiroseovarius, are involved in vital ecological processes, including complex carbohydrate degradation, aerobic denitrification, assimilatory nitrate reduction, ammonium assimilation, and sulfur oxidation. Overall, our study reveals that the common practice of carbohydrate addition in biofloc aquaculture systems fosters the growth of specific heterotrophic bacterial communities, particularly Rhodobacteraceae. These bacteria contribute to maintaining water quality by removing toxic nitrogen and sulfur compounds and enhance animal health by colonizing gut microbiota and exerting probiotic effects.

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  • Effects of Red Kojic Rice Supplementation on Growth, Immunity, Antioxidant Capacity, and Intestinal Health of Litopenaeus vannamei Fed a Diet with Fish Meal Replacement by Soybean Meal
    Qianping Huang, Hongkai Ye, Zhanzhan Wang, Bo Liu, Min Yang, Xiaobin Chen, Shengli Liu, Chuanpeng Zhou
    Fishes.2026; 11(1): 58.     CrossRef
  • Egg disinfection improves larval survival and shapes the microbial community in snubnose pompano (Trachinotus blochii)
    T. G. Sumithra, S. R. Krupesha Sharma, S. Gayathri, Ambarish P. Gop, K. S. Shravana, Amritha Jagannivasan, Anusree V. Nair, K. S. Sudarsan, B. Santhosh, Sanal Ebeneezar, A. Gopalakrishnan
    Scientific Reports.2026;[Epub]     CrossRef
  • Unifying microorganisms and macrograzers in intertidal rocky shore ecological networks
    Clara Arboleda‐Baena, Claudia Belén Pareja, Javiera Poblete, Eric L. Berlow, Hugo Sarmento, Ramiro Logares, Rodrigo De la Iglesia, Sergio A. Navarrete
    Ecology.2026;[Epub]     CrossRef
  • Ecological disposal of bauxite tailings and red mud: A sustainable strategy for bauxite industrial waste reuse
    Xusheng Jiang, Xuehong Zhang, Xijun Liu, Hui Qiu, Mengting Lin, Guo Yu, Shouhui Zhang, Jie Liu
    Resources, Conservation and Recycling.2025; 218: 108259.     CrossRef
  • Variation of Microorganisms and Water Quality, and Their Impacts on the Production of Penaeus vannamei in Small-Scale Greenhouse Ponds
    Siyu Wu, Haochang Su, Lei Su, Yucheng Cao, Guoliang Wen, Yu Xu, Bin Shen, Shanshan Wu, Yuting Su, Xiaojuan Hu
    Microorganisms.2025; 13(3): 546.     CrossRef
  • Effect of hydraulic retention time of sponge-based trickling filter for shrimp culture recirculating tank
    Penpicha Satanwat, Mami Nagai, Tharin Boonprasertsakul, Akihiro Nagano, Tsutomu Okubo, Nur Adlin, Takahiro Watari, Masashi Hatamoto, Takashi Yamaguchi, Sitthakarn Sitthi, Wiboonluk Pungrasmi, Rapeepun Vanichviriyakit, Sorawit Powtongsook
    Process Safety and Environmental Protection.2025; 198: 107154.     CrossRef
  • Divergence patterns of bacterial communities between larviculture systems of two Penaeus vannamei strains with distinct culture traits
    Heng Chen, Fengdie Zhang, Junqi Yu, Rudan Chen, Demin Zhang, Chen Chen, Kai Wang
    Aquaculture.2025; 606: 742572.     CrossRef
  • Influence of Polyester and Denim Microfibers on the Treatment and Formation of Aerobic Granules in Sequencing Batch Reactors
    Victoria Okhade Onyedibe, Hassan Waseem, Hussain Aqeel, Steven N. Liss, Kimberley A. Gilbride, Roxana Sühring, Rania Hamza
    Processes.2025; 13(7): 2272.     CrossRef
  • Where Have You Been? Backtracking Microplastic to Its Source Using the Biomolecular Composition of the Ecocorona
    Amanda L. Dawson, Utpal Bose, Sophia Escobar-Correas, Kuok Yap, David J. Craik, Cassandra L. Pegg
    Environmental Science & Technology.2025; 59(41): 22227.     CrossRef
  • Effects of butyric acid on the inhibition of Vibrio parahaemolyticus and its impacts on growth performance, intestinal microbiota, and water microbiota in Litopenaeus vannamei
    Minghan Yin, Zhangyi Zheng, Ruiyan Xing, Lefei Jiao, Moussa Gouife, Tinghong Ming, Fei Kong, Shuang Qiu, Jiajie Xu
    Aquaculture Reports.2025; 45: 103078.     CrossRef
  • Evaluating the potential of Rhodopseudomonas faecalis PA2 as a probiotic and biofloc supplement for Nile tilapia (Oreochromis niloticus)
    Wipavee Thaimuangphol, Ranumas Sukkum, Zhongliang Wang, Chewapat Saejung
    Aquaculture Reports.2025; 45: 103201.     CrossRef
  • Valorization of Invasive Aquatic Weed (Eichhornia crassipes) Into a Sustainable Carbon Source in GIFT‐Based Biofloc Systems
    Megha Lovejan, K. M. Mujeeb Rahiman, C. G. Joshy, R. Vipina, Remy Ntakirutimana, Jianguang Qin
    Aquaculture Nutrition.2025;[Epub]     CrossRef
Mammaliicoccus sciuri's Pan-Immune System and the Dynamics of Horizontal Gene Transfer among Staphylococcaceae: a One-Health CRISPR Tale
Allan de Carvalho, Marcia Giambiagi-deMarval, Ciro César Rossi
J. Microbiol. 2024;62(9):775-784.   Published online July 22, 2024
DOI: https://doi.org/10.1007/s12275-024-00156-7
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AbstractAbstract PDF
Recently emancipated from the Staphylococcus genus due to genomic differences, Mammaliicoccus sciuri, previously classified as an occasional pathogen, emerges as a significant player in the landscape of resistance gene dissemination among Staphylococcaceae. Despite its classification, its role remained enigmatic. In this study, we delved into the genomic repertoire of M. sciuri to unravel its contribution to resistance and virulence gene transfer in the context of One Health. Through comprehensive analysis of publicly available genomes, we unveiled a diverse pan-immune system adept at defending against exogenous genetic elements, yet concurrently fostering horizontal gene transfer (HGT). Specifically, exploration of CRISPR-Cas systems, with spacer sequences as molecular signatures, elucidated a global dissemination pattern spanning environmental, animal, and human hosts. Notably, we identified the integration of CRISPR-Cas systems within SCCmecs (Staphylococcal Cassette Chromosome mec), harboring key genes associated with pathogenicity and resistance, especially the methicillin resistance gene mecA, suggesting a strategic adaptation to outcompete other mobile genetic elements. Our findings underscored M. sciuri's active engagement in HGT dynamics and evolutionary trajectories within Staphylococcaceae, emphasizing its central role in shaping microbial communities and highlighting the significance of understanding its implications in the One Health framework, an interdisciplinary approach that recognizes the interconnectedness of human, animal, and environmental health to address global health challenges.

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  • Metagenomic Comparison of Bat Colony Resistomes Across Anthropogenic and Pristine Habitats
    Julio David Soto-López, Omar Velásquez-González, Manuel A. Barrios-Izás, Moncef Belhassen-García, Juan Luis Muñoz-Bellido, Pedro Fernández-Soto, Antonio Muro
    Antibiotics.2026; 15(1): 51.     CrossRef
  • From Farm to Community: Dispersal of Potentially Pathogenic Staphylococcus and Mammaliicoccus Species and Antimicrobial Resistance Across Shared Environments
    Faizan Ahmad, Samuel Sathler Martuchelle, Ana Luisa Andrade-Oliveira, Vitor Emanuel Lanes Viana, Maria Antônia Silva Melo Sousa, Felipe Sicchierolli da Silveira, Marisa Alves Nogueira-Diaz, Monalessa Fábia Pereira, Marcia Giambiagi-deMarval, Ciro César Ro
    Current Microbiology.2025;[Epub]     CrossRef
  • Genomic insights into multidrug and heavy metal resistance in Chryseobacterium sp. BI5 isolated from sewage sludge
    Mrinmoy Patra, Anand Kumar Pandey, Suresh Kumar Dubey
    Total Environment Microbiology.2025; 1(1): 100005.     CrossRef
  • The Arms Race Between Actinobacillus pleuropneumoniae and Its Genetic Environment: A Comprehensive Analysis of Its Defensome and Mobile Genetic Elements
    Giarlã Cunha da Silva, Ciro César Rossi
    Molecular Microbiology.2025; 124(1): 40.     CrossRef
  • Defense systems and mobile elements in Staphylococcus haemolyticus: a genomic view of resistance dissemination
    Giarlã Cunha da Silva, Ciro César Rossi
    Microbial Pathogenesis.2025; 206: 107808.     CrossRef
  • Frequency, Distribution, and Antimicrobial Resistance of Methicillin-Resistant Staphylococci and Mammaliicoccus sciuri Isolated from Dogs and Their Owners in Rio de Janeiro
    Fernanda Cruz Bonnard, Luciana Guimarães, Izabel Mello Teixeira, Sandryelle Mercês Freire, Alessandra Maia, Patrícia Câmara de Castro Abreu Pinto, Thais Veiga Blanchart, Bruno Penna
    Antibiotics.2025; 14(4): 409.     CrossRef
  • From farm effluent to biotechnological potential: pGLS, a novel and resilient temperate bacteriophage with synergistic activity and broad antibiofilm properties against Staphylococcus and Mammaliicoccus
    Vitor Emanuel Lanes Viana, Faizan Ahmad, Samuel Sathler Martuchelle, Sandy de Almada Estanislau, Nohman Rasheed, Marinella Silva Laport, Monalessa Fábia Pereira, Marcia Giambiagi-deMarval, Ciro César Rossi
    Journal of Applied Microbiology.2025;[Epub]     CrossRef
  • Staphylococcus parequorum sp. nov. and Staphylococcus halotolerans sp. nov., isolated from traditional Korean soybean foods
    Ju Hye Baek, Dong Min Han, Dae Gyu Choi, Chae Yeong Moon, Jae Kyeong Lee, Chul-Hong Kim, Jung-Woong Kim, Che Ok Jeon
    Journal of Microbiology.2025; 63(8): e2503003.     CrossRef
  • Discovery of phage CSF, a novel generalist bacteriophage targeting multidrug-resistant and potentially pathogenic Staphylococcus spp. and Mammaliicoccus spp.
    Faizan Ahmad, Vitor Emanuel Lanes Viana, Rafael Reis de Rezende, Samuel Sathler Martuchelle, Anderson Souza Cabral, Ana Luisa Andrade-Oliveira, Isabella Monteiro Carvalho, Sandy de Almada Estanislau, Nohman Rasheed, Poliane Alfenas Zerbini, Monalessa Fábi
    Archives of Virology.2025;[Epub]     CrossRef
  • Characterization of Phylogenetically Distinct Temperate Phages from Kenyan Mammaliicoccus sciuri
    Jérémy D.R. Cherbuin, Jaime Llodrá, Loïc Borcard, Sabine Kaessmeyer, Alban Ramette, Javier Eduardo Fernandez, Theresa Maria Wagner, Sergi Torres-Puig, Peter Kuhnert, Dann Turner, Fabien Labroussaa, Jörg Jores
    PHAGE.2025; 6(4): 259.     CrossRef
  • Human Pathogenic Bacteria Within the Nasal and Rectal Microbiome of Macropus giganteus
    David Arroyo, Amy Peart, Brian Vesely, Andrew Trudgian, Jessica Chellappah
    Tropical Medicine and Infectious Disease.2025; 10(11): 322.     CrossRef
Cultivation of Diverse Novel Marine Bacteria from Deep Ocean Sediment Using Spent Culture Supernatant of Ca. Bathyarchaeia Enrichment
Sidra Erum Ishaq, Tariq Ahmad, Lewen Liang, Ruize Xie, Tiantian Yu, Yinzhao Wang, Fengping Wang
J. Microbiol. 2024;62(8):611-625.   Published online July 10, 2024
DOI: https://doi.org/10.1007/s12275-024-00145-w
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AbstractAbstract PDF
Most microorganisms resist pure cultivation under conventional laboratory conditions. One of the primary issues for this un-culturability is the absence of biologically produced growth-promoting factors in traditionally defined growth media. However, whether cultivating microbes by providing spent culture supernatant of pivotal microbes in the growth medium can be an effective approach to overcome this limitation is still an under-explored area of research. Here, we used the spent culture medium (SCM) method to isolate previously uncultivated marine bacteria and compared the efficiency of this method with the traditional cultivation (TC) method. In the SCM method, Ca. Bathyarchaeia-enriched supernatant (10%) was used along with recalcitrant organic substrates such as lignin, humic acid, and organic carbon mixture. Ca. Bathyarchaeia, a ubiquitous class of archaea, have the capacity to produce metabolites, making their spent culture supernatant a key source to recover new bacterial stains. Both cultivation methods resulted in the recovery of bacterial species from the phyla Pseudomonadota, Bacteroidota, Actinomycetota, and Bacillota. However, our SCM approach also led to the recovery of species from rarely cultivated groups, such as Planctomycetota, Deinococcota, and Balneolota. In terms of the isolation of new taxa, the SCM method resulted in the cultivation of 80 potential new strains, including one at the family, 16 at the genus, and 63 at the species level, with a novelty ratio of ~ 35% (80/219). In contrast, the TC method allowed the isolation of ~ 10% (19/171) novel strains at species level only. These findings suggest that the SCM approach improved the cultivation of novel and diverse bacteria.

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  • Engineering the phycosphere: fundamental concepts and tools for the bottom-up design of microalgal-bacterial consortia
    Austin Semple, Jagroop Pandhal
    Applied Phycology.2025; 6(1): 21.     CrossRef
  • Darkness to Discovery: A Comprehensive Mini-Review on Culturable and Non-Culturable Microbial Diversity from Deep Sea
    Abhay B. Fulke, Nilkanth Sharma, Jayshree Nadekar
    Microbial Ecology.2025;[Epub]     CrossRef
  • The bacterial community of the freshwater bryozoan Cristatella mucedo and its secondary metabolites production potential
    Inmaculada Tocino-Márquez, Martin Zehl, Joana Séneca, Petra Pjevac, Manuel Felkl, Christian F. W. Becker, Alexander Loy, Thomas Rattei, Andrew N. Ostrovsky, Sergey B. Zotchev
    Scientific Reports.2025;[Epub]     CrossRef
  • Uncertainty Analysis of Biogas Generation and Gas Hydrate Accumulations in the Baiyun Sag, South China Sea
    Pibo Su, Jinqiang Liang, Huai Cheng, Yaoyao Lv, Wei Zhang, Zuofei Zhu
    Microorganisms.2024; 13(1): 5.     CrossRef
Enhancing Seed Germination of Cremastra appendiculata: Screening and Identification of Four New Symbiotic Fungi in the Psathyrellaceae Family
Zhangneng Pan, Jing Wang, Shanshan He, Haiyang Zhao, Xinyue Dong, Tao Feng, Yanyan Meng, Xiaojun Li
J. Microbiol. 2024;62(8):671-682.   Published online June 28, 2024
DOI: https://doi.org/10.1007/s12275-024-00148-7
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AbstractAbstract PDF
Several coprinoid fungi have been identified as promotors of Cremastra appendiculata seed germination, while others appear ineffective. This study aimed to discern which genera within the Psathyrellaceae family exhibit this capability and to identify the most effective coprinoid fungi for the cultivation of C. appendiculata. We collected 21 coprinoid fungi from diverse sources and symbiotically cultured them with C. appendiculata seeds. 9 fungi were found to induce seed germination and support seed development, specifically within the genera Coprinellus, Tulosesus, and Candolleomyces. In contrast, fungi that failed to promote germination predominantly belonged to the genera Coprinopsis and Parasola. Notably, four fungi-Coprinellus xanthothrix, Coprinellus pseudodisseminatus, Psathyrella singeri, and Psathyrella candolleana-were documented for the first time as capable of enhancing C. appendiculata seed germination. Strain 218LXJ-10, identified as Coprinellus radians, demonstrated the most significant effect and has been implemented in large-scale production, underscoring its considerable practical value. These findings contribute vital scientific insights for the conservation and sustainable use of C. appendiculata resources.

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  • Non-targeted metabolomic profiling of Cremastra appendiculata providing insights for phytochemical analyses
    Rui Guan, Yuxin Shan, Hamizah Shahirah Hamezah, Somnuk Bunsupa, Hong To Quyen Duong, Yadong Zhou, Rongchun Han, Xiaohui Tong
    PeerJ.2026; 14: e20592.     CrossRef
  • Culture conditions of symbiotic fungus Coprinellus radians and its effects on seedlings of Cremastra appendiculata (D.Don.) Makino (Orchidaceae)
    Liqin Wang, Jingyi Zhang, Qiuyu Lv, Gonggu Lv, Guangquan Lv, Xin Xiao, Fulin Yan, Miao Liu, Mingsheng Zhang
    Canadian Journal of Plant Science.2025; 105: 1.     CrossRef
  • Solid vermicompost and its liquid derivative exhibit strong biocontrol properties against Myzus persicae aphids on sweet pepper
    Mey Jerbi-Elayed, Stefan Brandl, Grégory Sempo, John Riviere, Deborah Lanterbecq, Marie Annet, Leidy Grawez Demoulin, Muriel Quinet, Stanley Lutts, Thierry Hance, François Renoz
    Journal of Pest Science.2025; 98(4): 2265.     CrossRef
Delineating the Acquired Genetic Diversity and Multidrug Resistance in Alcaligenes from Poultry Farms and Nearby Soil
Abhilash Bhattacharjee, Anil Kumar Singh
J. Microbiol. 2024;62(7):511-523.   Published online June 21, 2024
DOI: https://doi.org/10.1007/s12275-024-00129-w
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  • 18 Download
  • 1 Web of Science
  • 1 Crossref
AbstractAbstract PDF
Alcaligenes faecalis is one of the most important and clinically significant environmental pathogens, increasing in importance due to its isolation from soil and nosocomial environments. The Gram-negative soil bacterium is associated with skin endocarditis, bacteremia, dysentery, meningitis, endophthalmitis, urinary tract infections, and pneumonia in patients. With emerging antibiotic resistance in A. faecalis, it has become crucial to understand the origin of such resistance genes within this clinically significant environmental and gut bacterium. In this research, we studied the impact of antibiotic overuse in poultry and its effect on developing resistance in A. faecalis. We sampled soil and faecal materials from five poultry farms, performed whole genome sequencing & analysis and identified four strains of A. faecalis. Furthermore, we characterized the genes in the genomic islands of A. faecalis isolates. We found four multidrug-resistant A. faecalis strains that showed resistance against vancomycin (MIC >1000 μg/ml), ceftazidime (50 μg/ml), colistin (50 μg/ml) and ciprofloxacin (50 μg/ml). From whole genome comparative analysis, we found more than 180 resistance genes compared to the reference sequence. Parts of our assembled contigs were found to be similar to different bacteria which included pbp1A and pbp2 imparting resistance to amoxicillin originally a part of Helicobacter and Bordetella pertussis. We also found the Mycobacterial insertion element IS6110 in the genomic islands of all four genomes. This prominent insertion element can be transferred and induce resistance to other bacterial genomes. The results thus are crucial in understanding the transfer of resistance genes in the environment and can help in developing regimes for antibiotic use in the food and poultry industry.

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  • First Case of Respiratory Infection in Rabbits Caused by Alcaligenes faecalis in Romania
    Vlad Iorgoni, Ionica Iancu, Ionela Popa, Alexandru Gligor, Gabriel Orghici, Bogdan Sicoe, Corina Badea, Cristian Dreghiciu, Călin Pop, Timea Bochiș, Janos Degi, Luminita Costinar, Corina Pascu, Viorel Herman
    Veterinary Sciences.2025; 12(1): 33.     CrossRef
Autotrophy to Heterotrophy: Shift in Bacterial Functions During the Melt Season in Antarctic Cryoconite Holes
Aritri Sanyal, Runa Antony, Gautami Samui, Meloth Thamban
J. Microbiol. 2024;62(8):591-609.   Published online May 30, 2024
DOI: https://doi.org/10.1007/s12275-024-00140-1
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AbstractAbstract PDF
Microbes residing in cryoconite holes (debris, water, and nutrient-rich ecosystems) on the glacier surface actively participate in carbon and nutrient cycling. Not much is known about how these communities and their functions change during the summer melt-season when intense ablation and runoff alter the influx and outflux of nutrients and microbes. Here, we use high-throughput-amplicon sequencing, predictive metabolic tools and Phenotype MicroArray techniques to track changes in bacterial communities and functions in cryoconite holes in a coastal Antarctic site and the surrounding fjord, during the summer season. The bacterial diversity in cryoconite hole meltwater was predominantly composed of heterotrophs (Proteobacteria) throughout the season. The associated functional potentials were related to heterotrophic-assimilatory and -dissimilatory pathways. Autotrophic Cyanobacterial lineages dominated the debris community at the beginning and end of summer, while heterotrophic Bacteroidota- and Proteobacteria-related phyla increased during the peak melt period. Predictive functional analyses based on taxonomy show a shift from predominantly phototrophy-related functions to heterotrophic assimilatory pathways as the melt-season progressed. This shift from autotrophic to heterotrophic communities within cryoconite holes can affect carbon drawdown and nutrient liberation from the glacier surface during the summer. In addition, the flushing out and export of cryoconite hole communities to the fjord could influence the biogeochemical dynamics of the fjord ecosystem.

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  • Biogeography of Cryoconite Bacterial Communities Across Continents
    Qianqian Ge, Zhiyuan Chen, Yeteng Xu, Wei Zhang, Guangxiu Liu, Tuo Chen, Binglin Zhang
    Microorganisms.2026; 14(1): 162.     CrossRef
  • Post-glacial microbial succession and carbon sequestration processes: insights from recent research
    Wenmei Li, Shichang Kang, Wasim Sajjad
    Environmental Sciences Europe.2025;[Epub]     CrossRef
  • Extensive Genomic Rearrangement of Catalase-Less Cyanobloom-Forming Microcystis aeruginosa in Freshwater Ecosystems
    Minkyung Kim, Jaejoon Jung, Wonjae Kim, Yerim Park, Che Ok Jeon, Woojun Park
    Journal of Microbiology.2024; 62(11): 933.     CrossRef
Review
Reverse Zoonotic Transmission of SARS-CoV-2 and Monkeypox Virus: A Comprehensive Review
Chiranjib Chakraborty, Manojit Bhattacharya, Md Aminul Islam, Hatem Zayed, Elijah Ige Ohimain, Sang-Soo Lee, Prosun Bhattacharya, Kuldeep Dhama
J. Microbiol. 2024;62(5):337-354.   Published online May 23, 2024
DOI: https://doi.org/10.1007/s12275-024-00138-9
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AbstractAbstract PDF
Reverse zoonosis reveals the process of transmission of a pathogen through the human-animal interface and the spillback of the zoonotic pathogen. In this article, we methodically demonstrate various aspects of reverse zoonosis, with a comprehensive discussion of SARS-CoV-2 and MPXV reverse zoonosis. First, different components of reverse zoonosis, such as humans, different pathogens, and numerous animals (poultry, livestock, pets, wild animals, and zoo animals), have been demonstrated. Second, it explains the present status of reverse zoonosis with different pathogens during previous occurrences of various outbreaks, epidemics, and pandemics. Here, we present 25 examples from literature. Third, using several examples, we comprehensively illustrate the present status of the reverse zoonosis of SARS-CoV-2 and MPXV. Here, we have provided 17 examples of SARS-CoV-2 reverse zoonosis and two examples of MPXV reverse zoonosis. Fourth, we have described two significant aspects of reverse zoonosis: understanding the fundamental aspects of spillback and awareness. These two aspects are required to prevent reverse zoonosis from the current infection with two significant viruses. Finally, the One Health approach was discussed vividly, where we urge scientists from different areas to work collaboratively to solve the issue of reverse zoonosis.

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  • Phylogenetic analyses of the spread of Clade I MPOX in African and non-African nations
    Chiranjib Chakraborty, Manojit Bhattacharya, Arpita Das, Ali S. Abdelhameed
    Virus Genes.2025; 61(3): 265.     CrossRef
  • Efficient and modular reverse genetics system for rapid generation of recombinant severe acute respiratory syndrome coronavirus 2
    Sojung Bae, Jinjong Myoung
    Journal of Microbiology.2025; 63(7): e2504015.     CrossRef
  • Real-time malaria detection in the Amazon rainforest via drone-collected eDNA and portable qPCR
    Yin Cheong Aden Ip, Luca Montemartini, Jia Jin Marc Chang, Andrea Desiderato, Nicolás D. Franco-Sierra, Christian Geckeler, Mailyn Adriana Gonzalez Herrera, Michele Gregorini, Meret Jucker, Steffen Kirchgeorg, Martina Lüthi, Elvira Mächler, Frederik Bendi
    One Health.2025; 21: 101167.     CrossRef
  • One digital health through wearables: a viewpoint on human–pet integration towards Healthcare 5.0
    Mostafa Haghi, Samira Abani, Soheil Khooyooz, Anice Jahanjoo, Samaneh Rashidibajgan, Nima TaheriNejad, Thomas M. Deserno, Holger Volk
    Frontiers in Digital Health.2025;[Epub]     CrossRef
  • Development of a multiplex real-time PCR for the simultaneous detection of monkeypox virus clades I, II, and goatpox virus
    Yongqiang Lin, Zijing Guo, Jinsong Chen, Xianwen Zhang, Long Zhou, Yanmin Li, Zhidong Zhang
    Frontiers in Veterinary Science.2024;[Epub]     CrossRef
  • Differential Impact of Spike Protein Mutations on SARS-CoV-2 Infectivity and Immune Evasion: Insights from Delta and Kappa Variants
    Tae-Hun Kim, Sojung Bae, Jinjong Myoung
    Journal of Microbiology and Biotechnology.2024; 34(12): 2506.     CrossRef
Journal Article
Medium Chain Length Polyhydroxyalkanoate Production by Engineered Pseudomonas gessardii Using Acetate-formate as Carbon Sources
Woo Young Kim, Seung-Jin Kim, Hye-Rin Seo, Yoonyong Yang, Jong Seok Lee, Moonsuk Hur, Byoung-Hee Lee, Jong-Geol Kim, Min-Kyu Oh
J. Microbiol. 2024;62(7):569-579.   Published online May 3, 2024
DOI: https://doi.org/10.1007/s12275-024-00136-x
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AbstractAbstract PDF
Production of medium chain length polyhydroxyalkanoate (mcl-PHA) was attempted using Pseudomonas gessardii NIBRBAC000509957, which was isolated from Sunchang, Jeollabuk-do, Republic of Korea (35°24'27.7"N, 127°09'13.0"E) and effectively utilized acetate and formate as carbon sources. We first evaluated the utilization of acetate as a carbon source, revealing optimal growth at 5 g/L acetate. Then, formate was supplied to the acetate minimal medium as a carbon source to enhance cell growth. After overexpressing the acetate and formate assimilation pathway enzymes, this strain grew at a significantly higher rate in the medium. As this strain naturally produces PHA, it was further engineered metabolically to enhance mcl-PHA production. The engineered strain produced 0.40 g/L of mcl-PHA with a biomass content of 30.43% in fed-batch fermentation. Overall, this strain can be further developed to convert acetate and formate into valuable products.

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  • A modular single- and dual-gene expression toolkit for Kluyveromyces marxianus
    Zewei Lu, Changhui Lv, Zhuoer Chen, Zhiwei Zhu, Xiaoqiang Ma
    World Journal of Microbiology and Biotechnology.2026;[Epub]     CrossRef
  • Formate-driven photoautotrophic growth and biopolymer storage in anaerobic purple bacteria
    Mohammad Adib Ghazali Abdul Rahman, Bronwyn Laycock, Steven Pratt, Damien J. Batstone
    Bioresource Technology.2025; 434: 132753.     CrossRef
  • Sulphide and oleic acid synergism in accelerating mcl-PHA biopolymer production in Pseudomonas aeruginosa MCC 5300 by modulating electron transport system
    Raghavendra Paduvari, Divyashree Somashekara
    Biochemistry and Biophysics Reports.2025; 44: 102286.     CrossRef
  • Unlocking efficient polyhydroxyalkanoate production by Gram-positive Priestia megaterium using waste-derived feedstocks
    Xinyi Bai, Libo Xu, Kang Li, Guangbao Zhang, Mengjun Zhang, Yi Huang
    Microbial Cell Factories.2025;[Epub]     CrossRef
  • Selective utilization of formic acid and acetic acid in succinic acid fermentation broth to produce single-cell protein using Rhodotorula glutinis
    Fuqiang Liu, Pengfei Wu, Lin Yu, Zitu Lü, Xinying Sun, Jiaxin Li, Lei Liu, Jing Wu, Jianan Zhang
    Bioprocess and Biosystems Engineering.2025;[Epub]     CrossRef
Review
Balancing Act of the Intestinal Antimicrobial Proteins on Gut Microbiota and Health
Ye Eun Ra, Ye‑Ji Bang
J. Microbiol. 2024;62(3):167-179.   Published online April 17, 2024
DOI: https://doi.org/10.1007/s12275-024-00122-3
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  • 27 Download
  • 18 Web of Science
  • 19 Crossref
AbstractAbstract PDF
The human gut houses a diverse and dynamic microbiome critical for digestion, metabolism, and immune development, exerting profound efects on human health. However, these microorganisms pose a potential threat by breaching the gut barrier, entering host tissues, and triggering infections, uncontrolled infammation, and even sepsis. The intestinal epithelial cells form the primary defense, acting as a frontline barrier against microbial invasion. Antimicrobial proteins (AMPs), produced by these cells, serve as innate immune efectors that regulate the gut microbiome by directly killing or inhibiting microbes. Abnormal AMP production, whether insufcient or excessive, can disturb the microbiome equilibrium, contributing to various intestinal diseases. This review delves into the complex interactions between AMPs and the gut microbiota and sheds light on the role of AMPs in governing host-microbiota interactions. We discuss the function and mechanisms of action of AMPs, their regulation by the gut microbiota, microbial evasion strategies, and the consequences of AMP dysregulation in disease. Understanding these complex interactions between AMPs and the gut microbiota is crucial for developing strategies to enhance immune responses and combat infections within the gut microbiota. Ongoing research continues to uncover novel aspects of this intricate relationship, deepening our understanding of the factors shaping gut health. This knowledge has the potential to revolutionize therapeutic interventions, ofering enhanced treatments for a wide range of gut-related diseases.

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  • Microbiota-derived D-amino acids in intestinal homeostasis and inflammatory bowel disease
    Kentaro Miyamoto, Tomohisa Sujino
    Inflammation and Regeneration.2026;[Epub]     CrossRef
  • Natural and Synthetic Peptides as Alternatives to Antibiotics in Intestinal Infections—A Review
    Lala Stepanyan, Monika Israyelyan, Alessandro Gori, Avetis Tsaturyan, Zhaklina Saribekyan, Kristina Hovsepyan, Tatevik Sargsyan, Raffaele Pastore, Antonio De Luca, Giovanni N. Roviello
    Antibiotics.2026; 15(1): 68.     CrossRef
  • Host–Microbiota Interactions in Obesity: Immune Metabolic Dysregulation
    Ning Zhihan, Gou Weiyu, Gan Luo, Yang Hong
    Obesity Reviews.2026;[Epub]     CrossRef
  • Host-directed therapies modulating innate immunity against infection in hematologic malignancies
    Qiong Wang, Kristján Hermannsson, Egill Másson, Peter Bergman, Guðmundur Hrafn Guðmundsson
    Blood Reviews.2025; 70: 101255.     CrossRef
  • Progress in the Identification and Design of Novel Antimicrobial Peptides Against Pathogenic Microorganisms
    Shengwei Sun
    Probiotics and Antimicrobial Proteins.2025; 17(2): 918.     CrossRef
  • Comparison of naturalization mouse model setups uncover distinct effects on intestinal mucosa depending on microbial experience
    Henriette Arnesen, Signe Birkeland, Harriet Stendahl, Klaus Neuhaus, David Masopust, Preben Boysen, Harald Carlsen
    Discovery Immunology.2025;[Epub]     CrossRef
  • Oral administration of LEAP2 enhances immunity against Edwardsiella tarda through regulation of gut bacterial community and metabolite in mudskipper
    Ting-Fang Zhu, Hai-Peng Guo, Li Nie, Jiong Chen
    Fish & Shellfish Immunology.2025; 158: 110128.     CrossRef
  • Pharmacology of Intestinal Inflammation and Repair
    Céline Deraison, Nathalie Vergnolle
    Annual Review of Pharmacology and Toxicology .2025; 65(1): 301.     CrossRef
  • Microbiome dysbiosis in SARS-CoV-2 infection: implication for pathophysiology and management strategies of COVID-19
    Shukur Wasman Smail, Niaz Albarzinji, Rebaz Hamza Salih, Kalthum Othman Taha, Sarah Mousa Hirmiz, Hero M. Ismael, Marwa Fateh Noori, Sarkar Sardar Azeez, Christer Janson
    Frontiers in Cellular and Infection Microbiology.2025;[Epub]     CrossRef
  • Harnessing the Microbiome: CRISPR-Based Gene Editing and Antimicrobial Peptides in Combating Antibiotic Resistance and Cancer
    Radwa A. Amen, Yaser M. Hassan, Rawan A. Essmat, Rana H. Ahmed, Marwan M. Azab, Nadia R. Shehata, Mariam M. Elgazzar, Wael M. El-Sayed
    Probiotics and Antimicrobial Proteins.2025; 17(4): 1938.     CrossRef
  • The Role of Functional Feed in Modulating Fish Gut Microbiome to Enhance Resistance Against Aquaculture Pathogens
    Eswar Marcharla, A Vishnuprasadh, Lalitha Gnanasekaran, Saranya Vinayagam, Thanigaivel Sundaram, Swamynathan Ganesan
    Probiotics and Antimicrobial Proteins.2025;[Epub]     CrossRef
  • Metabolic profiling and genetic tool development in the mucosal bacterium Selenomonas sputigena
    Trinh Thi Nguyen, Yu-Kyung Kim, Trang Vu Thien Nguyen, Junbeom Kwon, Ye-Ji Bang
    Genes & Genomics.2025; 47(10): 997.     CrossRef
  • Impact of the COVID-19 pandemic on vancomycin-resistant Enterococcus bloodstream infections: a 6-year study in Western Greece
    Maria Lagadinou, Christos Michailides, Christodoulos Chatzigrigoriadis, Ioannis Erginousakis, Prodromos Avramidis, Marina Amerali, Fotini Tasouli, Anna Chondroleou, Katerina Skintzi, Anastasia Spiliopoulou, Fevronia Kolonitsiou, Leonidia Leonidou, Stelios
    Frontiers in Microbiology.2025;[Epub]     CrossRef
  • Modeling gut inflammation using intestinal organoids: Advances, challenges, and future perspectives
    Justina Guzauskiene, Deimante Valentelyte, Goda Butaite, Ugne Kulokiene, Viltaute Laukaitiene, Ruta Inciuraite, Jurgita Skieceviciene
    Best Practice & Research Clinical Gastroenterology.2025; 78: 102048.     CrossRef
  • Rational bioengineering of polysaccharide in designing of microbiome modulation
    Jeong Hyun Moon, Kidong Kim, Yubin Kim, Sejin Son
    Trends in Biotechnology.2025;[Epub]     CrossRef
  • Swine-Derived Probiotics and Their Metabolites as an Alternative to Veterinary Antibiotics
    Mengshi Zhao, Bihong Chen, Song Peng, Guiheng Mei, Meiqin Li, Fengqiang Lin, Tiecheng Sun, Zhaolong Li
    Veterinary Sciences.2025; 12(11): 1100.     CrossRef
  • Macrophages and Gut Barrier Function: Guardians of Gastrointestinal Health in Post-Inflammatory and Post-Infection Responses
    Edward Xiangtai Meng, George Nicholas Verne, Qiqi Zhou
    International Journal of Molecular Sciences.2024; 25(17): 9422.     CrossRef
  • Host-Associated Microbiome
    Woo Jun Sul
    Journal of Microbiology.2024; 62(3): 135.     CrossRef
  • Gut Microbiota as Emerging Players in the Development of Alcohol-Related Liver Disease
    Wei Li, Wenkang Gao, Shengqi Yan, Ling Yang, Qingjing Zhu, Huikuan Chu
    Biomedicines.2024; 13(1): 74.     CrossRef
Journal Articles
Antimicrobial Efficacy of Allium cepa and Zingiber officinale Against the Milk‑Borne Pathogen Listeria monocytogenes
Abirami Arasu , Nagaram Prabha , Durga Devi , Praveen Kumar Issac , Khaloud Mohammed Alarjani , Dunia A. Al Farraj , Reem A. Aljeidi , Dina S. Hussein , Magesh Mohan , Jehad Zuhair Tayyeb , Ajay Guru , Jesu Arockiaraj
J. Microbiol. 2023;61(11):993-1011.   Published online December 4, 2023
DOI: https://doi.org/10.1007/s12275-023-00086-w
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  • 3 Download
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AbstractAbstract PDF
Listeria monocytogenes is an important food-borne pathogen that causes listeriosis and has a high case fatality rate despite its low incidence. Medicinal plants and their secondary metabolites have been identified as potential antibacterial substances, serving as replacements for synthetic chemical compounds. The present studies emphasize two significant medicinal plants, Allium cepa and Zingiber officinale, and their efficacy against L. monocytogenes. Firstly, a bacterial isolate was obtained from milk and identified through morphology and biochemical reactions. The species of the isolate were further confirmed through 16S rRNA analysis. Furthermore, polar solvents such as methanol and ethanol were used for the extraction of secondary metabolites from A. cepa and Z. officinale. Crude phytochemical components were identified using phytochemical tests, FTIR, and GC–MS. Moreover, the antibacterial activity of the crude extract and its various concentrations were tested against L. monocytogenes. Among all, A. cepa in methanolic extracts showed significant inhibitory activity. Since, the A. cepa for methanolic crude extract was used to perform autography to assess its bactericidal activity. Subsequently, molecular docking was performed to determine the specific compound inhibition. The docking results revealed that four compounds displayed strong binding affinity with the virulence factor Listeriolysin-O of L. monocytogenes. Based on the above results, it can be concluded that the medicinal plant A. cepa has potential antibacterial effects against L. monocytogenes, particularly targeting its virulence.

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  • Enhancing raw trout fillet quality with Lactiplantibacillus plantarum postbiotics and potassium sorbate
    Amin Abbasi, Hadi Pourjafar, Mahdi Asghari Ozma, Mansoureh Taghizadeh, Hedayat Hosseini
    Letters in Applied Microbiology.2025;[Epub]     CrossRef
  • Cultural Perspectives on the Sustainable Use and Added Value of Plant-Based Food Dyes—A Case Study from Bulgaria
    Mihail Chervenkov, Teodora Ivanova, Yulia Bosseva, Dessislava Dimitrova
    Sustainability.2024; 16(20): 9049.     CrossRef
Quantitative Analysis of RNA Polymerase Slippages for Production of P3N‑PIPO Trans‑frame Fusion Proteins in Potyvirids
Dongjin Choi , Yoonsoo Hahn
J. Microbiol. 2023;61(10):917-927.   Published online October 16, 2023
DOI: https://doi.org/10.1007/s12275-023-00083-z
  • 441 View
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  • 6 Web of Science
  • 6 Crossref
AbstractAbstract PDF
Potyvirids, members of the family Potyviridae, produce the P3N-PIPO protein, which is crucial for the cell-to-cell transport of viral genomic RNAs. The production of P3N-PIPO requires an adenine (A) insertion caused by RNA polymerase slippage at a conserved GAA AAA A ( GA6) sequence preceding the PIPO open reading frame. Presently, the slippage rate of RNA polymerase has been estimated in only a few potyvirids, ranging from 0.8 to 2.1%. In this study, we analyzed publicly available plant RNA-seq data and identified 19 genome contigs from 13 distinct potyvirids. We further investigated the RNA polymerase slippage rates at the GA6 motif. Our analysis revealed that the frequency of the A insertion variant ranges from 0.53 to 4.07% in 11 potyviruses (genus Potyvirus). For the two macluraviruses (genus Macluravirus), the frequency of the A insertion variant was found to be 0.72% and 10.96% respectively. Notably, the estimated RNA polymerase slippage rates for 12 out of the 13 investigated potyvirids were reported for the first time in this study. Our findings underscore the value of plant RNA-seq data for quantitative analysis of potyvirid genome variants, specifically at the GA6 slippage site, and contribute to a more comprehensive understanding of the RNA polymerase slippage phenomenon in potyvirids.

Citations

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  • Discovery of novel tepovirus genomes with a nucleic acid-binding protein homolog by systematic analysis of plant transcriptome data
    Dongjin Choi, Hyerin Park, Seungwoo Baek, Myeung Seok Choi, Sylvain Legay, Gea Guerriero, Jean-François Hausman, Yoonsoo Hahn
    Acta Virologica.2025;[Epub]     CrossRef
  • Expanding the diversity of Celavirus, the most divergent genus in the family Potyviridae
    Myeung Seok Choi, Yoonsoo Hahn
    Virus Genes.2025; 61(6): 758.     CrossRef
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    De-Jie Cheng, Xin-Yang Chen, Carlos Kwesi Tettey, Song-Yu Jiang, Jun Jiang, Cheng-Wu Zou, Hai-Tao Cui, Yan-Ping Tian, Xiang-Dong Li
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    Adrian A. Valli, María Luisa Domingo-Calap, Alfonso González de Prádena, Juan Antonio García, Hongguang Cui, Cécile Desbiez, Juan José López-Moya, Shou-Wei Ding, Andrew Firth
    mBio.2024;[Epub]     CrossRef
Ultrasonic Treatment Enhanced Astaxanthin Production of Haematococcus pluvialis
Yun Hwan Park , Jaewon Park , Jeong Sik Choi , Hyun Soo Kim , Jong Soon Choi , Yoon-E Choi
J. Microbiol. 2023;61(6):633-639.   Published online June 13, 2023
DOI: https://doi.org/10.1007/s12275-023-00053-5
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AbstractAbstract PDF
In this study, effects of ultrasonic treatment on Haematococcus pluvialis (H. pluvialis) were investigated. It has been confirmed that the ultrasonic stimulation acted as stress resources in the red cyst stage H. pluvialis cells containing astaxanthin,
result
ing in additional astaxanthin production. With the increase in production of astaxanthin, the average diameter of H. pluvialis cells increased accordingly. In addition, to determine how ultrasonic stimulation had an effect on the further biosynthesis of astaxanthin, genes related to astaxanthin synthesis and cellular ROS level were measured. As a result, it was confirmed that astaxanthin biosynthesis related genes and cellular ROS levels were increased, and thus ultrasonic stimulation acts as an oxidative stimulus. These results support the notion on the effect of the ultrasonic treatment, and we believe our novel approach based on the ultrasonic treatment would help to enhance the astaxanthin production from H. pluvialis.

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    Federico Ortenzi, Federico Montereali, Saverio Savio, Alberta Di Cave, Alessandro Contaldo, Simonetta Antonaroli, Blasco Morozzo della Rocca, Roberta Congestri
    Bioresource Technology.2025; 434: 132810.     CrossRef
  • Utilization of Microalgae and Duckweed as Sustainable Protein Sources for Food and Feed: Nutritional Potential and Functional Applications
    Yingjie Song, Zhangli Hu, Sizhao Liu, Shasha Luo, Ruimin He, Xinyan Yang, Shuang Li, Xuewei Yang, Yuxing An, Yinglin Lu
    Journal of Agricultural and Food Chemistry.2025; 73(8): 4466.     CrossRef
  • Multidimensional Characterization of the Physiological State of Hematococcuspluvialis Using Scanning Structured Illumination Super-Resolution Microscopy
    Meiting Wang, Yifeng Deng, Yuye Wang, Jiajie Chen, Xinran Li, Peng Du, Xiaomin Zheng, Junle Qu, Bruce Zhi Gao, Xiao Peng, Yonghong Shao
    Analytical Chemistry.2025; 97(8): 4379.     CrossRef
  • Transition between germination and dormancy for non-motile cells of Haematococcus pluvialis: dependence on nitrogen availability through metabolic flux of amino acids and nucleotides
    Jing Li, Litao Zhang, Wenjie Yu, Feng Chen, Anatoly A. Tsygankov, Jianguo Liu
    Bioresource Technology.2025; 434: 132818.     CrossRef
  • Haematococcus pluvialis culture contaminated with chytrids: growth management and astaxanthin production
    Zineb Mansouri, Ibtissam Lijassi, Ana Amorim, Aïcha El Aissami, Laila Rhazi, Imane Wahby
    Antonie van Leeuwenhoek.2025;[Epub]     CrossRef
  • Microalgae-based nutritional supplements: Sustainable applications for high-nutritional-value food production
    Ricky Rajamanickam, Satwika Das, Chandukishore T, Shagun Sharma, Rajesh R O, Ashish A. Prabhu, Sanjukta Banerjee, Nur Izyan Wan Azelee, Sankaran Krishnamoorthy, Rangabhashiyam Selvasembian
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  • Optimization of astaxanthin extraction from red (Gracilaria corticata) and brown (Sargassum polycystum) macroalgae through ultrasonication and microwave processing
    Parisa Feizi, Seid Mahdi Jafari
    Ultrasonics Sonochemistry.2025; 121: 107556.     CrossRef
  • Recent Advances in Astaxanthin as an Antioxidant in Food Applications
    Yimeng Dang, Zhixi Li, Fanqianhui Yu
    Antioxidants.2024; 13(7): 879.     CrossRef
  • Effect of reduced atmospheric pressures on the morphology and astaxanthin biosynthesis of microalga Haematococcus lacustris
    Sangui Kim, Rendi Mahadi, Aditya Lakshmi Narasimhan, Catherine Christabel, Hyoji Yu, Eui-Jin Kim, You-Kwan Oh
    Biotechnology and Bioprocess Engineering.2024; 29(6): 1131.     CrossRef
Ship Hull‑Fouling Diatoms on Korean Research Vessels Revealed by Morphological and Molecular Methods, and Their Environmental Implications
Jaeyeong Park , Taehee Kim , Buhari Lawan Muhammad , Jang-Seu Ki
J. Microbiol. 2023;61(6):615-626.   Published online May 25, 2023
DOI: https://doi.org/10.1007/s12275-023-00055-3
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AbstractAbstract PDF
Ship biofouling is one of the main vectors for the introduction and global spread of non-indigenous organisms. Diatoms were the early colonizers of ship hulls; however, their community composition on ships is poorly understood. Herein, we investigated the diatom community on the hull samples collected from two Korean research vessels Isabu (IRV) and Onnuri (ORV) on September 2 and November 10, 2021, respectively. IRV showed low cell density (345 cells/cm2) compared to ORV (778 cells/cm2). We morphologically identified more than 15 species of diatoms from the two research vessels (RVs). The microalgae in both RVs were identified as Amphora, Cymbella, Caloneis, Halamphora, Navicula, Nitzschia, and Plagiogramma. Of them, the genus Halamphora was found to be predominant. However, both RVs had a varied dominant species with a significant difference in body size; Halamphora oceanica dominated at IRV, and Halamphora sp. at ORV, respectively. Molecular cloning showed similar results to morphological analysis, in which Halamphora species dominated in both RVs. The hull-attached species were distinct from species found in the water column. These results revealed diatoms communities that are associated with ship hull-fouling at an early stage of biofilm formation. Moreover, ships arriving from different regions could show some variation in species composition on their hull surfaces, with the potential for nonindigenous species introduction.

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  • Halamphora navalis sp. nov. (Amphipleuraceae, Bacillariophyta), a new hull-fouling diatom from the research vessel Jangmok No. 1, Korea
    Taehee Kim, Joon Sang Park, Seung Ho Baek, Han-Sol Kim, Jang-Seu Ki
    Diatom Research.2026; : 1.     CrossRef
  • Invasion and ecological impact of the biofouling tube worm Hydroides elegans (Polychaeta: Serpulidae) in Korean coastal waters
    Sang Lyeol Kim, Ok Hwan Yu
    Frontiers in Marine Science.2025;[Epub]     CrossRef
  • Assessment of ship hull-attached marine diatoms: species composition and reattachment
    Jaeyeong Park, Buhari Lawan Muhammad, Taehee Kim, Quynh Thi Nhu Bui, Han-Sol Kim, Kyoungsoon Shin, Bonggil Hyun, Jung-Hoon Kang, Jang-Seu Ki
    Marine Pollution Bulletin.2025; 216: 117962.     CrossRef
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    Buhari Lawan Muhammad, Jinho Chae, Jang-Seu Ki
    Marine Biodiversity.2024;[Epub]     CrossRef
Description of Luteibacter aegosomatis sp. nov., Luteibacter aegosomaticola sp. nov., and Luteibacter aegosomatissinici sp. nov. isolated from the Intestines of Aegosoma sinicum Larvae
Hae-In Joe , Jee-Won Choi , June-Young Lee , Hojun Sung , Su-Won Jeong , Yun-Seok Jeong , Jae-Yun Lee , Jin-Woo Bae
J. Microbiol. 2023;61(6):603-613.   Published online May 5, 2023
DOI: https://doi.org/10.1007/s12275-023-00051-7
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AbstractAbstract PDF
Three novel bacterial strains, 321T, 335T, and 353T, were isolated from the intestines of Aegosoma sinicum larvae collected from Paju-Si, South Korea. The strains were Gram-negative, obligate aerobe and had rod-shaped cells with a single flagellum. The three strains belonged to the genus Luteibacter in the family Rhodanobacteraceae and shared < 99.2% similarity in their 16S rRNA gene sequence and < 83.56% similarity in thier whole genome sequence. Strains 321T, 335T, and 353T formed a monophyletic clade with Luteibacter yeojuensis KACC 11405T, L. anthropi KACC 17855T, and L. rhizovicinus KACC 12830T, with sequence similarities of 98.77–98.91%, 98.44–98.58%, and 97.88–98.02%, respectively. Further genomic analyses, including the construction of the Up-to-date Bacterial Core Gene (UBCG) tree and assessment of other genome-related indices, indicated that these strains were novel species belonging to the genus Luteibacter. All three strains contained ubiquinone Q8 as their major isoprenoid quinone and iso-C15:0 and summed feature 9 ( C16:0 10-methyl and/or iso-C17:1 ω9c) as their major cellular fatty acids. Phosphatidylethanolamine and diphosphatidylglycerol were the major polar lipids in all the strains. The genomic DNA G + C contents of strains 321T, 335T, and 353T were 66.0, 64.5, and 64.5 mol%, respectively. Based on multiphasic classification, strains 321T, 335T, and 353T were classified into the genus Luteibacter as the type strains of novel species, for which the names Luteibacter aegosomatis sp. nov., Luteibacter aegosomaticola sp. nov., and Luteibacter aegosomatissinici sp. nov. are proposed, respectively.

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  • First metagenome- and metatranscriptome dataset of Thecaphora frezzii teliospores, assembly and annotation of a new bacterial genome
    Renee S. Arias, John T. Dobbs, Valerie A. Orner, E. Cinthia Conforto, Alejandro M. Rago, Luis I. Cazon, Victor S. Sobolev, Imana L. Power, Marshall C. Lamb, Alicia N. Massa
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  • Luteibacter sahnii sp. nov., A Novel Yellow-Colored Xanthomonadin Pigment Producing Probiotic Bacterium from Healthy Rice Seed Microbiome
    Gagandeep Jaiswal, Rekha Rana, Praveen Kumar Nayak, Rekha Chouhan, Sumit G. Gandhi, Hitendra K. Patel, Prabhu B. Patil
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    Aharon Oren, Markus Göker
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Vaginal Microbiome Dysbiosis is Associated with the Different Cervical Disease Status
Yingying Ma , Yanpeng Li , Yanmei Liu , Le Cao , Xiao Han , Shujun Gao , Chiyu Zhang
J. Microbiol. 2023;61(4):423-432.   Published online April 3, 2023
DOI: https://doi.org/10.1007/s12275-023-00039-3
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AbstractAbstract PDF
Vaginal microbiome composition was demonstrated to be associated with cervical disease. The colonization characteristics of vaginal microbes and their association with the different cervical disease status, especially cervical cancer (CC), are rarely investigated. In this cross-sectional study, we characterized the vaginal microbiome of women with different status of cervical diseases, including 22 NV + (normal tissue with HPV infection), low-grade squamous intraepithelial lesion (LSIL, n = 45), high-grade squamous intraepithelial lesion (HSIL, n = 36) and CC (n = 27) using bacterial 16S DNA sequencing. Thirty HPV-negative women with normal tissue were used as the control group. We found that higher diversity of microbiome with gradual depletion of Lactobacillus, especially L. crispatus, was associated with the severity of cervical disease. High-risk HPV16 infection was associated with higher microbiome diversity and depletion of Lactobacillus in high-grade cervical diseases (i.e. HSIL and CC). The CC group was characterized by higher levels of Fannyhessea vaginae, Prevotella, Bacteroides, Finegoldia, Vibrio, Veillonella, Peptostreptococcus, and Dialister. Co-occurrence network analyses showed that negative correlations were exclusively observed between Lactobacillus and other bacteria, and almost all non-Lactobacillus bacteria were positively correlated with each other. In particular, the most diverse and complex co-occurrence network of vaginal bacteria, as well as a complete loss of L. crispatus, was observed in women with CC. Logistic regression model identified HPV16 and Lactobacillus as significant risk and protective factors for CC, respectively. These results suggest that specific Lactobacillus species (e.g. L. crispatus and L. iners) can be used as important markers to target prevention measures prioritizing HPV16-infected women and other hrHPV-infected women for test, vaccination and treat initiatives.

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    María del Carmen Lagunas-Cruz, Arturo Valle-Mendiola, Isabel Soto-Cruz
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    Na He, Cunjian Yi, Qingsong Zeng, Wumei Jing, Wenrong He
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    Giosuè Giordano Incognito, Carlo Ronsini, Vittorio Palmara, Paola Romeo, Giuseppe Vizzielli, Stefano Restaino, Marco La Verde, Orazio De Tommasi, Marco Palumbo, Stefano Cianci
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  • Associations of the gut, cervical, and vaginal microbiota with cervical cancer: a systematic review and meta-analysis
    Qin Wen, Shubin Wang, Yalan Min, Xinyi Liu, Jian Fang, Jinyi Lang, Meihua Chen
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    Vivian Heimbecker, Bárbara Pontarollo Dal Santos, Ana Paula Thomaz, Keite da Silva Nogueira, Camila Marconi
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    BMC Infectious Diseases.2025;[Epub]     CrossRef
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    Hrishikesh D Pai, Rashmi Baid, Nandita P Palshetkar, Rishma Pai, Arnav Pai, Rohan Palshetkar
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    Volha P. Lohinava, Natalia I. Shevchenko, Elena L. Gasich
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    Angeliki Gerede, Konstantinos Nikolettos, Eleftherios Vavoulidis, Chrysoula Margioula-Siarkou, Stamatios Petousis, Maria Giourga, Panagiotis Fotinopoulos, Maria Salagianni, Sofoklis Stavros, Konstantinos Dinas, Nikolaos Nikolettos, Ekaterini Domali
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    Marwa Saadaoui, Parul Singh, Osman Ortashi, Souhaila Al Khodor
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Epidemiological Characteristics of Norovirus Outbreaks in Shenyang from 2017 to 2021
Ying Qi , Xinxin Dong , Xiaowei Cheng , Han Xu , Jin Wang , Bing Wang , Ye Chen , Baijun Sun , Linlin Zhang , Yan Yao
J. Microbiol. 2023;61(4):471-478.   Published online March 27, 2023
DOI: https://doi.org/10.1007/s12275-023-00033-9
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AbstractAbstract PDF
Norovirus is one of the leading causes of acute gastroenteritis outbreaks worldwide. This study aimed to identify the epidemiological characteristics of norovirus outbreaks and to provide evidence for public health entities. Specimens and epidemiological survey data were collected to determine if there were differences in the attack rate of norovirus in terms of the year, season, transmission route, exposure setting, and region and to determine whether there were relationships between the reporting interval, the number of illnesses in a single outbreak and the duration of the outbreak. Norovirus outbreaks were reported throughout the year, with seasonal characteristics (i.e., high rates in spring and winter). Among all regions in Shenyang with the exception of Huanggu and Liaozhong, norovirus outbreaks had been reported, and the primary genotype was GII.2[P16]. Vomiting was the most common symptom. The main places of occurrence were childcare institutions and schools. The person-to-person route was the main transmission route. The median duration of norovirus was 3 days (IQR [interquartile range]: 2–6 days), the median reporting interval was 2 days (IQR: 1–4 days), the median number of illnesses in a single outbreak was 16 (IQR: 10–25); there was a positive correlation between these parameters. Norovirus surveillance and genotyping studies still need to be further strengthened to increase knowledge regarding the pathogens and their variant characteristics, to better characterize the patterns of norovirus outbreaks and to provide information for outbreak prevention. Norovirus outbreaks should be detected, reported and handled early. Public health entities and the government should develop corresponding measures for different seasons, transmission routes, exposure settings, and regions.

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    Yuan Tian, Xiqiao Du, Hong Gao, Mingyue Yuan, Yingchen Wang, Lei Shang, Yuhui Pan, Tuo Dong, Zhe Zhang
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    Junshan Gao, Zilei Zhang, Yang Jiao, Yinghuan Xu, Yuanling Chen, Liang Xue
    Science of The Total Environment.2025; 989: 179854.     CrossRef
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    Junhong Ai, Qiliang Li, Ke Xu, Yuxuan Li, Ying Liu, Luci Huang, Wenqi Song, Zhengde Xie
    Virology Journal.2025;[Epub]     CrossRef
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    Euncheol Son, Young-Hoon Kim
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  • Development and Evaluation of a Rapid GII Norovirus Detection Method Based on CRISPR-Cas12a
    Xinyi Hu, Pei He, Tong Jiang, Jilu Shen
    Polish Journal of Microbiology.2024; 73(1): 89.     CrossRef
  • Improving knowledge, attitude and practice on norovirus infection diarrhea among staff of kindergartens and schools: a before-after study
    Hongxin Lyu, Dongmei Liang, Riyan Luo, Yunlong Feng, Lei Liu, Sixia Yang, Fuling Cai, Zhen Zhang, Huawei Xiong
    BMC Public Health.2024;[Epub]     CrossRef
  • Epidemiological and Molecular Genetic Analysis of Outbreaks of Acute Intestinal Infections in the Khabarovsk Krai in 2022
    Elena Yu. Sapega, Liudmila V. Butakova, Olga E. Trotsenko, Tatyana A. Zaitseva, Tatyana N. Karavyanskaya
    ЗДОРОВЬЕ НАСЕЛЕНИЯ И СРЕДА ОБИТАНИЯ - ЗНиСО / PUBLIC HEALTH AND LIFE ENVIRONMENT.2023; : 74.     CrossRef
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Reviews
Membrane Proteins as a Regulator for Antibiotic Persistence in Gram‑Negative Bacteria
Jia Xin Yee , Juhyun Kim , Jinki Yeom
J. Microbiol. 2023;61(3):331-341.   Published online February 17, 2023
DOI: https://doi.org/10.1007/s12275-023-00024-w
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AbstractAbstract PDF
Antibiotic treatment failure threatens our ability to control bacterial infections that can cause chronic diseases. Persister bacteria are a subpopulation of physiological variants that becomes highly tolerant to antibiotics. Membrane proteins play crucial roles in all living organisms to regulate cellular physiology. Although a diverse membrane component involved in persistence can result in antibiotic treatment failure, the regulations of antibiotic persistence by membrane proteins has not been fully understood. In this review, we summarize the recent advances in our understanding with regards to membrane proteins in Gram-negative bacteria as a regulator for antibiotic persistence, highlighting various physiological mechanisms in bacteria.

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    Mabrouk Sobhy, Tamer Elsamahy, Esraa A. Abdelkarim, Ebtihal Khojah, Haiying Cui, Lin Lin
    Microbial Pathogenesis.2025; 205: 107661.     CrossRef
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    Yunzhu Xu, Dahai Hou, Min Zhao, Tong Zhao, Yong Ma, Yafeng Zhang, Yang Guo, Weiwei Tao, Hui Wang
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  • PhoPQ-mediated lipopolysaccharide modification governs intrinsic resistance to tetracycline and glycylcycline antibiotics in Escherichia coli
    Byoung Jun Choi, Umji Choi, Dae-Beom Ryu, Chang-Ro Lee, Mehrad Hamidian, You-Hee Cho
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  • Bacterial Regulatory Mechanisms for the Control of Cellular Processes: Simple Organisms’ Complex Regulation
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The “Cins” of Our Fathers: Rejuvenated Interest in Colicins to Combat Drug Resistance
Sumudu Upatissa , Robert J. Mitchell
J. Microbiol. 2023;61(2):145-158.   Published online February 8, 2023
DOI: https://doi.org/10.1007/s12275-023-00023-x
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AbstractAbstract PDF
With the growing threat of antibiotic resistance, researchers around the globe are seeking alternatives to stem bacterial pathogenesis. One such alternative is bacteriocins, proteins produced by bacterial species to inhibit the growth and viability of related bacterial species. With their diverse mechanisms, which include pore formation and nuclease activities, and narrow spectrum of activities, which limit their impact to only certain bacterial species, unlike many chemical antibiotics, bacteriocins offer intriguing possibilities to selectively control individual bacterial populations. Within this review, therefore, we highlight current research exploring the application of colicins and microcins, a subset of bacteriocins, with an emphasis on their activities against drug-resistant pathogens, both in in vitro and in vivo settings.

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    R. Askarali, Jelin Vilvest, Alex Yagoo, A. Mariya Vaishnika
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    Microorganisms.2023; 11(7): 1800.     CrossRef
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    Sumudu Upatissa, Wonsik Mun, Robert J. Mitchell, Minsu Kim
    Microbiology Spectrum.2023;[Epub]     CrossRef
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    Veronika S. Mihailovskaya, Dmitry A. Sutormin, Marina O. Karipova, Anna B. Trofimova, Victor A. Mamontov, Konstantin Severinov, Marina V. Kuznetsova
    International Journal of Molecular Sciences.2023; 24(16): 12636.     CrossRef
Journal Articles
Chryseobacterium paludis sp. nov. and Chryseobacterium foetidum sp. nov. Isolated from the Aquatic Environment, South Korea
Miryung Kim , Yong Seok Kim , Chang Jun Cha
J. Microbiol. 2023;61(1):37-47.   Published online February 1, 2023
DOI: https://doi.org/10.1007/s12275-022-00008-2
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AbstractAbstract PDF
Two novel bacterial species CJ51T and CJ63T belonging to the genus Chryseobacterium were isolated from the Upo wetland and the Han River, South Korea, respectively. Cells of these strains were Gram-stain-negative, aerobic, non-motile, rodshaped, and catalase- and oxidase-positive. Both strains were shown to grow optimally at 30 °C and pH 7 in the absence of NaCl on tryptic soy agar. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains CJ51T and CJ63T belonged to the genus Chryseobacterium and were most closely related to Chryseobacterium piperi CTMT and Chryseobacterium piscicola VQ-6316sT with 98.47% and 98.46% 16S rRNA sequence similarities, respectively. The average nucleotide identity values of strains CJ51T and CJ63T with its closely related type strains Chryseobacterium piperi CTMT and Chryseobacterium piscicola VQ-6316sT were 81.9% and 82.1%, respectively. The major fatty acids of strains CJ51T and CJ63T were iso-C15:0, iso-C17:0 3-OH and summed feature 9 ( C16:0 10-methyl and/or iso-C17:1ω9c). Menaquinone 6 (MK-6) was identified as the primary respiratory quinone in both strains. The major polar lipids of strains CJ51T and CJ63T were phosphatidylethanolamine and several unidentified amino lipids and lipids. Based on polyphasic taxonomy data, strains CJ51T and CJ63T represent novel species of the genus Chryseobacterium, for which names Chryseobacterium paludis sp. nov. and Chryseobacterium foetidum sp. nov. are proposed respectively. The type strains are CJ51T (= KACC 22749T = JCM 35632T) and CJ63T (= KACC 22750T = JCM 35633T).

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    Hee-Won Bae, Hyeong-Jun Ki, Shin-Yae Choi, You-Hee Cho, Kristin N. Parent
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  • Chryseobacterium cupriresistens sp. nov., a copper-resistant bacterium isolated from soil contaminated with heavy metals in Chapala Basin, Mexico
    Ivan Arroyo-Herrera, Ana Laura Reséndiz-Martínez, Brenda Román-Ponce, Joseph Guevara-Luna, Xiaoxia Zhang, Ayixon Sánchez-Reyes, Paulina Estrada-de los Santos, En Tao Wang, María Soledad Vásquez-Murrieta
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  • Validation List no. 212. Valid publication of new names and new combinations effectively published outside the IJSEM
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Functional analysis of ascP in Aeromonas veronii TH0426 reveals a key role in the regulation of virulence
Yongchao Guan , Meng Zhang , Yingda Wang , Zhongzhuo Liu , Zelin Zhao , Hong Wang , Dingjie An , Aidong Qian , Yuanhuan Kang , Wuwen Sun , Xiaofeng Shan
J. Microbiol. 2022;60(12):1153-1161.   Published online November 10, 2022
DOI: https://doi.org/10.1007/s12275-022-2373-8
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AbstractAbstract PDF
Aeromonas veronii is a pathogen which can induce diseases in humans, animals and aquatic organisms, but its pathogenic mechanism and virulence factors are still elusive. In this study, we successfully constructed a mutant strain (ΔascP) by homologous recombination. The results showed that the deletion of the ascP gene significantly down-regulated the expression of associated effector proteins in A. veronii compared to its wild type. The adhesive and invasive abilities of ΔascP to EPC cells were 0.82-fold lower in contrast to the wild strain. The toxicity of ΔascP to cells was decreased by about 2.91-fold (1 h) and 1.74-fold (2 h). Furthermore, the LD50 of the mutant strain of crucian carp was reduced by 19.94-fold, and the virulence was considerably attenuated. In contrast to the wild strain, the ΔascP content in the liver and spleen was considerably lower. The titers of serum cytokines (IL-8, TNF-α, and IL-1β) in crucian carp after the infection of the ΔascP strain were considerably lower in contrast to the wild strain. Hence, the ascP gene is essential for the etiopathogenesis of A. veronii TH0426.

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  • Complete genome sequence and genome-wide transposon mutagenesis enable the determination of genes required for sodium hypochlorite tolerance and drug resistance in pathogen Aeromonas veronii GD2019
    Yifan Bu, Chengyu Liu, Yabo Liu, Wensong Yu, Tingjin Lv, Yuanxing Zhang, Qiyao Wang, Yue Ma, Shuai Shao
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    Yang Li, Chao Liu, Yuechen Sun, Ruijun Wang, Choufei Wu, Hanqu Zhao, Liqin Zhang, Dawei Song, Quanxin Gao
    International Journal of Biological Macromolecules.2024; 259: 129395.     CrossRef
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    Jin-shuo Gong, Ying-da Wang, Yan-long Jiang, Di Zhang, Ya-nan Cai, Xiao-feng Shan, He Gong, Hao Dong
    Aquaculture.2024; 580: 740361.     CrossRef
Review
[Minireview]Biodegradation of plastics: mining of plastic-degrading microorganisms and enzymes using metagenomics approaches
Dae-Wi Kim , Jae-Hyung Ahn , Chang-Jun Cha
J. Microbiol. 2022;60(10):969-976.   Published online September 27, 2022
DOI: https://doi.org/10.1007/s12275-022-2313-7
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AbstractAbstract PDF
Plastic pollution exacerbated by the excessive use of synthetic plastics and its recalcitrance has been recognized among the most pressing global threats. Microbial degradation of plastics has gained attention as a possible eco-friendly countermeasure, as several studies have shown microbial metabolic capabilities as potential degraders of various synthetic plastics. However, still defined biochemical mechanisms of biodegradation for the most plastics remain elusive, because the widely used culture-dependent approach can access only a very limited amount of the metabolic potential in each microbiome. A culture-independent approach, including metagenomics, is becoming increasingly important in the mining of novel plastic-degrading enzymes, considering its more expanded coverage on the microbial metabolism in microbiomes. Here, we described the advantages and drawbacks associated with four different metagenomics approaches (microbial community analysis, functional metagenomics, targeted gene sequencing, and whole metagenome sequencing) for the mining of plastic-degrading microorganisms and enzymes from the plastisphere. Among these approaches, whole metagenome sequencing has been recognized among the most powerful tools that allow researchers access to the entire metabolic potential of a microbiome. Accordingly, we suggest strategies that will help to identify plastisphere-enriched sequences as de novo plastic-degrading enzymes using the whole metagenome sequencing approach. We anticipate that new strategies for metagenomics approaches will continue to be developed and facilitate to identify novel plastic-degrading microorganisms and enzymes from microbiomes.

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Journal Articles
Deletion of lacD gene affected stress tolerance and virulence of Streptococcus suis serotype 2
Xiaowu Jiang , Lexin Zhu , Dongbo Zhan
J. Microbiol. 2022;60(9):948-959.   Published online August 19, 2022
DOI: https://doi.org/10.1007/s12275-022-2146-4
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AbstractAbstract PDF
Streptococcus suis type 2 (S. suis type 2, SS2), an infectious pathogen which is zoonotic and can induce severely public health concern. Our previous research identified a newly differential secreted effector of tagatose-bisphosphate aldolase (LacD) mediated by VirD4 factor within the putative type IV secretion system of SS2, whereas the functional basis and roles in virulence of LacD remain elusive. Here in this study, the LacD was found enzymatic and can be activated to express under oxidative stress. Gene mutant and its complemental strain (ΔlacD and cΔlacD) were constructed to analyze the phenotypes, virulence and transcriptomic profiles as compared with the parental strain. The lacD gene deletion showed no effect on growth capability and cells morphology of SS2. However, reduced tolerance to oxidative and heat stress conditions, increased antimicrobial susceptibility to ciprofloxacin and kanamycin were found in ΔlacD strain. Further, the LacD deficiency led to weakened invasion and attenuated virulence since an easier phagocytosed and more prone to be cleared of SS2 in macrophages were shown in ΔlacD mutant. Distinctive transcriptional profiling in ΔlacD strain and typical downregulated genes with significant mRNA changes including alcohol dehydrogenase, GTPase, integrative and conjugative elements, and iron ABC transporters which were mainly involved in cell division, stress response, antimicrobial susceptibility and virulence regulation, were examined and confirmed by RNA sequencing and real time qPCR. In summary, the
results
demonstrated for the first time that LacD was a pluripotent protein mediated the metabolic, stress and virulent effect of SS2.

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  • Antimicrobial and antivirulence function of cinnamaldehyde against Streptococcus suis type 2
    Lexin Zhu, Zhishu He, Mengqing Li, Jixin Xu, Wei Ding, Wenzhen Zeng, Xiaowu Jiang, Artem S. Rogovsky
    Microbiology Spectrum.2025;[Epub]     CrossRef
  • Mechanisms Underlying the Effects of Secretory Protein G22 on Biological Characteristics and Virulence of Streptococcus suis
    Shiyue Fan, Yanping Tan, Zhiwei Li, Yanyan Zhang, Jinquan Li, Ye Feng, Yi He, Xiaoling Chen, Xingxing Dong
    Microorganisms.2025; 13(4): 774.     CrossRef
  • Investigation of choline-binding protein of CbpD in the pathogenesis of Streptococcus suis type 2
    Lexin Zhu, Mengqing Li, Guijun Yu, Dongbo Zhan, Wenzhen Zeng, Nanyan Fu, Xiaowu Jiang
    Frontiers in Veterinary Science.2024;[Epub]     CrossRef
Characterization of Marinilongibacter aquaticus gen. nov., sp. nov., a unique marine bacterium harboring four CRISPR-Cas systems in the phylum Bacteroidota
Dao-Feng Zhang , Yu-Fang Yao , Hua-Peng Xue , Zi-Yue Fu , Xiao-Mei Zhang , Zongze Shao
J. Microbiol. 2022;60(9):905-915.   Published online August 1, 2022
DOI: https://doi.org/10.1007/s12275-022-2102-3
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AbstractAbstract PDF
A novel bacterium, designated YYF0007T, was isolated from an agar-degrading co-culture. The strain was found harboring four CRISPR-Cas systems of two classes in the chromosome and subsequently subjected to a study on polyphasic taxonomy. Pairwise analyses of the 16S rRNA gene sequences indicated that strain YYF0007T had highest 16S rRNA gene sequence similarity (92.2%) to Jiulongibacter sediminis JN- 14-9T. The phylogenomic trees based on the 16S rRNA gene and 269 single-copy orthologous gene clusters (OCs) indicated that strain YYF0007T should be recognized as a novel genus of the family Spirosomaceae. The cells were Gramstain- negative, nonmotile, strictly aerobic, and straight long rods with no flagellum. Optimum growth occurred at 28°C and pH 7.0 with the presence of NaCl concentration 1.0–3.0% (w/v). The strain showed oxidase and catalase activities. The major fatty acids were C16:1ω5c, iso-C15:0 and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The predominant isoprenoid quinone was MK-7. The complete genome size was 4.64 Mb with a DNA G + C content of 44.4%. Further typing of CRISPR-Cas systems in the family Spirosomaceae and the phylum Bacteroidota indicated that it was remarkable for strain YYF0007T featured by such a set of CRISPR-Cas systems. This trait highlights the applications of strain YYF- 0007T in studies on the evolutionary dynamics and bacterial autoimmunity of CRISPR-Cas system as a potential model. The name Marinilongibacter aquaticus gen. nov., sp. nov. is proposed, and the type strain is YYF0007T (= MCCC 1K06017T = GDMCC 1.2428T = JCM 34683T).

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    Jiashun Li, Shuaishuai Wu, Kaidian Zhang, Xueqiong Sun, Wenwen Lin, Cong Wang, Senjie Lin
    Microorganisms.2024; 12(1): 118.     CrossRef
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    Yuqi Qi, Jun Zeng, Junshi Tao, Rentao Liu, Renchuan Fu, Chao Yan, Xiao Liu, Na Liu, Yanru Hao
    Chemosphere.2024; 351: 141174.     CrossRef
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    Huibin Lu, Li Chen, Linpei Huang
    International Journal of Systematic and Evolutionary Microbiology .2024;[Epub]     CrossRef
  • Thalassospira aquimaris sp. nov. and Winogradskyella marincola sp. nov. two marine bacteria isolated from an agar-degrading co-culture
    Zi-Yue Fu, Dao-Feng Zhang, Meng-Han Huang, Hong-Chuan Wang, Xiao-Ye Chen, Yu-Fang Yao, Yang Yuan, Wen-Jun Li
    Antonie van Leeuwenhoek.2024;[Epub]     CrossRef
  • Validation List no. 209. Valid publication of new names and new combinations effectively published outside the IJSEM
    Aharon Oren, Markus Göker
    International Journal of Systematic and Evolutionary Microbiology .2023;[Epub]     CrossRef
  • Telluribacter roseus sp. nov., Isolated from the Kumtag Desert Soil
    Chu-Ying Feng, Jia-Rui Han, Chun-Yan Lu, Li Gu, Shuai Li, Wen-Hui Lian, Lei Dong, Wen-Jun Li
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Helicobacter pylori-mediated gastric pathogenesis is attenuated by treatment of 2-deoxyglucose and metformin
Hanfu Su , Eun-Jung Bak , Aeryun Kim , Kavinda Tissera , Jeong-Heon Cha , Sungil Jang
J. Microbiol. 2022;60(8):849-858.   Published online June 22, 2022
DOI: https://doi.org/10.1007/s12275-022-2130-z
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AbstractAbstract PDF
Helicobacter pylori infection causes chronic inflammation in the stomach, which is linked to the development of gastric cancer. The anti-inflammatory and anticancer effects of a glycolysis inhibitor 2-deoxyglucose (2DG) and an antidiabetic medication metformin (Met) have gotten attention. Using a Mongolian gerbil animal model, we investigated H. pylorimediated gastric pathogenesis and how this pathogenesis is influenced by 2DG and Met. Five-week-old male gerbils were infected with H. pylori strain 7.13. After 2 weeks of infection, gerbils were fed 2DG-containing food (0.03% w/w), Met-containing water (0.5% w/v), or both (Combi) for 2 (short-term) or 10 weeks (long-term). Gastric pathogenesis and host response to H. pylori infection were examined by macroscopic and histopathologic analysis of gerbils’ stomach. As a result, indicators of gastric pathogenesis by H. pylori infection including infiltration of polymorphonuclear neutrophils and lymphocytes, intestinal metaplasia, atrophy, and proliferation of gastric epithelial cells were attenuated by short-term administration of 2DG, Met, or Combi. When the infection was sustained for long-term, gastric pathogenesis in drug-treated gerbils was equivalent to that in untreated gerbils, with the exception that the infiltration of neutrophil was reduced by 2DG. Colonization of H. pylori in stomach was unaffected by both short- and long-term treatments. Our findings demonstrate that the progression of gastric pathogenesis induced by H. pylori infection can be attenuated by the shortterm individual or combinational treatment of 2DG and Met, implying that 2DG or Met could be considered as a treatment option for gastric diseases in the early stages of infection.

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    Tai Zhang, Xudong Tang
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    Lingna Li, Jianing Huang, Tongmin Huang, Jie Yao, Yeyuan Zhang, Meiling Chen, Haojie Shentu, Haiying Lou, Flavia Prodam
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Dynamic colonization of gut microbiota and its influencing factors among the breast-feeding infants during the first two years of life
Ping Li , Xuelian Chang , Xiaoyu Chen , Tiantian Tang , Yajing Liu , Yu Shang , Kemin Qi
J. Microbiol. 2022;60(8):780-794.   Published online May 27, 2022
DOI: https://doi.org/10.1007/s12275-022-1641-y
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AbstractAbstract PDF
The maturation of infant gut microbiota has lifelong implications on health, which has been proposed as the major events during the first year of life. However, little is known about their dynamic colonization and influencing elements among the first two-year infancy as well as into the adulthood. So based on the 16S rRNA sequencing data among 30 healthy breast-feeding mother-infant pairs with normal ranges of growth and development indicators from birth to two years old, the dynamic colonization of gut microbiota and its influencing factors were discussed using this birth cohort. Among these, we identified that the diversity of gut microbiota was significantly increased from six-month to two-year subgroups. The significantly dynamic trends of gut microbiota at the phylum (genus) level were that the percents of Firmicutes (Faecalibacterium, Blautia, Enterococcus, Subdoligranulum, Agathobacter, unidentified_Erysipelotrichaceae, Staphylococcus, unidentified_Ruminococcaceae, and Fusicatenibacter), Bacteroidetes and Verrucomicrobia were increased, while Actinobacteria (Bifidobacterium) and Proteobacteria (unidentified- Enterobacteriaceae and Klebsiella) were decreased with the increased ages from six months to two years old, which might simultaneously modulate the host pathways, such as the higher percents of chemoheterotrophy and fermentation, and lower percentages of nitrate_reduction, aerobic_chemoheterotrophy and so on. Furthermore, there were significant associations between maternal (milk microbiota, pre-pregnancy BMI, BMI increment during the pregnancy)/infant characteristics (BMI at birth and BMI gain), and the compositions of gut microbiota. However, no differences of gut microbiota were shown between the different sex and productive mode subgroups. Overall, the colonization of gut microbiota is significantly matured into the adulthood with the increased ages to two-years old and regulated by the above maternal/infant characteristics, which will provide a new direction for the host-gut microbiota interplay during the first two years of life.

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  • Maternal separation during lactation affects recognition memory, emotional behaviors, hippocampus and gut microbiota composition in C57BL6J adolescent female mice
    Zuotian Wu, Lin Zhou, Huikang Fu, Yumeng Xie, Limin Sun, Yixin Li, Ling Xiao, Lei Zhang, Ying Su, Gaohua Wang
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    Eduard Flores Ventura, María Esteban-Torres, Miguel Gueimonde, Douwe van Sinderen, Omry Koren, Lindsay J. Hall, Nicola Segata, Mireia Valles-Colomer, Maria Carmen Collado
    npj Biofilms and Microbiomes.2025;[Epub]     CrossRef
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    Ruizheng Sun, Hao Chen, Siqi Yao, Zheng Yu, Chen Lai, Jing Huang
    Frontiers in Cellular and Infection Microbiology.2023;[Epub]     CrossRef
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Gamma-glutamyltransferase of Helicobacter pylori alters the proliferation, migration, and pluripotency of mesenchymal stem cells by affecting metabolism and methylation status
Zeyu Wang , Weijun Wang , Huiying Shi , Lingjun Meng , Xin Jiang , Suya Pang , Mengke Fan , Rong Lin
J. Microbiol. 2022;60(6):627-639.   Published online April 18, 2022
DOI: https://doi.org/10.1007/s12275-022-1575-4
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AbstractAbstract PDF
Virulence factor gamma-glutamyltransferase (GGT) of H. pylori consumes glutamine (Gln) in the stomach to decrease the tricarboxylic acid metabolite alpha-ketoglutarate (α-kg) and alter the downstream regulation of α-kg as well as cellular biological characteristics. Our previous research indicated that under H. pylori infection, mesenchymal stem cells (MSCs) migrated to the stomach and participated in gastric cancer (GC) development either by differentiating into epithelial cells or promoting angiogenesis. However, how MSCs themselves participate in H. pylori-indicated GC remains unclear. Therefore, a GGT knockout H. pylori strain (Hp- KS-1) was constructed, and downstream histone H3K9 and H3K27 methylation and the PI3K/AKT signaling pathway of α-kg were detected using Western blotting. The biological characteristics of MSCs were also examined. An additive α-kg supplement was also added to H. pylori-treated MSCs to investigate alterations in these aspects. Compared to the control and Hp-KS-1 groups, H. pylori-treated MSCs reduced Gln and α-kg, increased H3K9me3 and H3K27me3, activated the PI3K-AKT signaling pathway, and promoted the proliferation, migration, self-renewal, and pluripotency of MSCs. The addition of α-kg rescued the H. pylori-induced alterations. Injection of MSCs to nude mice resulted in the largest tumors in the H. pylori group and significantly reduced tumor sizes in the Hp-KS-1 and α-kg groups. In summary, GGT of H. pylori affected MSCs by interfering with the metabolite α-kg to increase trimethylation of histone H3K9 and H3K27, activating the PI3K/AKT signaling pathway, and promoting proliferation, migration, self-renewal, and pluripotency in tumorigenesis, elucidating the mechanisms of MSCs in GC development.

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    Héctor A. Tapia, Camila García‐Navarrete, Patricio Silva, Joaquín Lizana, Carla Fonfach, Ignacio Pezoa‐Soto, Tania Flores, Nadia Hernández, Daniel Peña‐Oyarzún, Jorge Toledo, Safka Hernández‐Gutiérrez, Daniela Herrera, Manuel Varas‐Godoy, Denisse Bravo, V
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    玉霞 龚
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    Jiefeng Huang, Shuangmeng Jia, Yitong Ji, Yingjia Zhu, Yishu Lu, Yiming Tang, Jiajie Yang, Guangpeng Liu, Lei Cui, Shuaijun Li
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    Fan Yang, Zhongbo Zhu, Lijuan Shi, Xiping Liu
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    Francesca Benedetti, Camila Summers, Robert C. Gallo, Davide Zella
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    Man Cui, Xiaohui Ji, Fengtao Guan, Guimin Su, Lin Du
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  • Gastric cancer and mesenchymal stem cell-derived exosomes: from pro-tumorigenic effects to anti-cancer vehicles
    Maryam Dolatshahi, Ahmad Reza Bahrami, Qaiser Iftikhar Sheikh, Mohsen Ghanbari, Maryam M. Matin
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  • Mesenchymal Stem Cell-Derived Exosomes Modulate Angiogenesis in Gastric Cancer
    Fawzy Akad, Veronica Mocanu, Sorin Nicolae Peiu, Viorel Scripcariu, Bogdan Filip, Daniel Timofte, Florin Zugun-Eloae, Magdalena Cuciureanu, Monica Hancianu, Teodor Oboroceanu, Laura Condur, Radu Florin Popa
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Randomized Controlled Trial
A split face study on the effect of an anti-acne product containing fermentation products of Enterococcus faecalis CBT SL-5 on skin microbiome modification and acne improvement
Hye Sung Han , Sun Hye Shin , Bo-Yun Choi , Nayeon Koo , Sanghyun Lim , Dooheon Son , Myung Jun Chung , Kui Young Park , Woo Jun Sul
J. Microbiol. 2022;60(5):488-495.   Published online March 14, 2022
DOI: https://doi.org/10.1007/s12275-022-1520-6
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AbstractAbstract PDF
Antibiotic-resistant Cutibacterium acnes and dysbiosis of the skin microbiome are of increasing concern in acne treatment. Enterococcus faecalis, a widely used probiotic, has shown benefits for acne treatment by exerting antimicrobial activity against C. acnes. Therefore, this study aimed to investigate the efficacy and safety of an E. faecalis CBT SL-5-extract-containing lotion in patients with mild-to-moderate acne. Twenty patients were enrolled in this randomized, placebo-controlled, split-face comparative study. Patients were treated with E. faecalis lotion on one side of the face and a vehicle lotion on the other side for 4 weeks. The efficacy outcome measures included improvement in the investigators’ assessment of acne severity, patient satisfaction, changes in skin parameters and diversity of the skin microbiome. The investigators’ assessment score was significantly improved on the test side compared to the control side, after 2 weeks (p = 0.009) and 6 weeks (p < 0.0005). However, TEWL and skin hydration were not significantly different between the two groups. The phylogenetic diversity of the skin microbiota decreased over time in the skin samples of test side. In conclusion, E. faecalis CBT SL-5 extract can be a feasible and well-tolerated option for improving acne severity and skin microbiome dysbiosis in mild-to-moderate acne patients.

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    Valentina Burckhardt-Bravo, Rodrigo Funes-Ferrada, Fernando Valenzuela
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  • Microbiome-Derived Indole-3-Lactic Acid Attenuates Cutibacterium Acnes-Induced Inflammation via the Aryl Hydrocarbon Receptor Pathway
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    Ida Ayu Manik Partha Sutema, Irma Latarissa, I Gusti Ayu Rai Widowati, Cynthia Retna Sartika, Ni Wayan Eka Ciptasari, Keri Lestari
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    Lanfang Zhang, Yuan Cai, Lin Li, Jie Hu, Changsha Jia, Xu Kuang, Yi Zhou, Zhiai Lan, Chunyan Liu, Feng Jiang, Nana Sun, Ni Zeng
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    Zhouna Li, Jianqing Zhang, Ying Zhang, Hong Chen, Yanqiu Bao
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    Cong Huang, Fan Zhuo, Baoquan Han, Wenting Li, Bin Jiang, Kaoyuan Zhang, Xingling Jian, Zhenzhen Chen, Hui Li, Haiyan Huang, Xia Dou, Bo Yu
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Journal Article
Gut microbiota metabolic characteristics in coronary artery disease patients with hyperhomocysteine
Ran Tian , Hong-Hong Liu , Si-Qin Feng , Yi-Fei Wang , Yi-Yang Wang , Yu-Xiong Chen , Hui Wang , Shu-Yang Zhang
J. Microbiol. 2022;60(4):419-428.   Published online March 4, 2022
DOI: https://doi.org/10.1007/s12275-022-1451-2
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AbstractAbstract PDF
Hyperhomocysteine (HHcy) is known as a risk factor for coronary artery disease (CAD). Despite the knowledge that gut microbiota related metabolism pathway shares metabolites with that of Hcy, little has been shown concerning the association between HHcy and gut microbiota. To explore their relationship in the context of CAD, 105 patients and 14 healthy controls were recruited from one single medical center located in Beijing, China. Their serum and fecal samples were collected, with multi-omics analyses performed via LC/MS/ MS and 16S rRNA gene V3-V4 region sequencing, respectively. Participants from the prospective cohort were divided into CAD, CAD & HHcy and healthy controls (HC) groups based on the diagnosis and serum Hcy concentration. The
results
revealed significant different metabolic signatures between CAD and CAD & HHcy groups. CAD patients with HHcy suffered a heavier atherosclerotic burden compared to CAD patients, and the difference was closely associated to betaine-homocysteine S-methyltransferase (BHMT)-related metabolites and trimethylamine N-oxide (TMAO)-related metabolites. Dimethylglycine (DMG) exhibited a strong positive correlation with serum total Hcy (tHcy), and TMAO and trimethylysine (TML) were associated with heavier atherosclerotic burden. Multiple other metabolites were also identified to be related to distinct cardiovascular risk factors. Additionally, Clostridium cluster IV and Butyricimonas were enriched in CAD patients with elevated tHcy. Our study suggested that CAD patients with elevated tHcy were correlated with higher atherosclerotic burden, and the impaired Hcy metabolism and cardiovascular risk were closely associated with BHMT-related metabolites, TMAO-related metabolites and impaired gut microbiota homeostasis.

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  • Homocysteine, Nutrition, and Gut Microbiota: A Comprehensive Review of Current Evidence and Insights
    Deborah Agostini, Alessia Bartolacci, Rossella Rotondo, Maria Francesca De Pandis, Michela Battistelli, Matteo Micucci, Lucia Potenza, Emanuela Polidori, Fabio Ferrini, Davide Sisti, Francesco Pegreffi, Valerio Pazienza, Edy Virgili, Vilberto Stocchi, Sab
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Review
Transmissibility and pathogenicity of SARS-CoV-2 variants in animal models
Young-Il Kim , Mark Anthony B. Casel , Young Ki Choi
J. Microbiol. 2022;60(3):255-267.   Published online March 2, 2022
DOI: https://doi.org/10.1007/s12275-022-2033-z
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AbstractAbstract PDF
As of February 2022, SARS-CoV-2 is still one of the most serious public health threats due to its high mortality rate and rapid spread of novel variants. Since the first outbreak in 2019, general understanding of SARS-CoV-2 has been improved through basic and clinical studies; however, knowledge gaps still exist in our understanding of the emerging novel SARSCoV- 2 variants, which impacts the corresponding development of vaccines and therapeutics. Especially, accumulation of mutations in SARS-CoV-2 and rapid spread in populations with previous immunity has resulted in selection of variants that evade the host immune response. This phenomenon threatens to render current SARS-CoV-2 vaccines ineffective for controlling the pandemic. Proper animal models are essential for detailed investigations into the viral etiology, transmission and pathogenesis mechanisms, as well as evaluation of the efficacy of vaccine candidates against recent SARS-CoV-2 variants. Further, the choice of animal model for each research topic is important for researchers to gain better knowledge of recent SARS-CoV-2 variants. Here, we review the advantages and limitations of each animal model, including mice, hamsters, ferrets, and non-human primates, to elucidate variant SARS-CoV-2 etiology and transmission and to evaluate therapeutic and vaccine efficacy.

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    Jinjong Myoung
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    Miruthula Tamil Selvan, Sachithra Gunasekara, Ping Xiao, Kristen Griffin, Shannon R. Cowan, Sai Narayanan, Akhilesh Ramachandran, Darren E. Hagen, Jerry W. Ritchey, Jennifer M. Rudd, Craig A. Miller
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Journal Articles
Structural and biochemical analysis of the PTPN4 PDZ domain bound to the C-terminal tail of the human papillomavirus E6 oncoprotein
Hye Seon Lee , Hye-Yeoung Yun , Eun-Woo Lee , Ho-Chul Shin , Seung Jun Kim , Bonsu Ku
J. Microbiol. 2022;60(4):395-401.   Published online January 28, 2022
DOI: https://doi.org/10.1007/s12275-022-1606-1
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AbstractAbstract PDF
High-risk genotypes of human papillomaviruses (HPVs) are directly implicated in various abnormalities associated with cellular hyperproliferation, including cervical cancer. E6 is one of two oncoproteins encoded in the HPV genome, which recruits diverse PSD-95/Dlg/ZO-1 (PDZ) domain-containing human proteins through its C-terminal PDZ-binding motif (PBM) to be degraded by means of the proteasome pathway. Among the three PDZ domain-containing protein tyrosine phosphatases, protein tyrosine phosphatase non-receptor type 3 (PTPN3) and PTPN13 were identified to be recognized by HPV E6 in a PBM-dependent manner. However, whether HPV E6 associates with PTPN4, which also has a PDZ domain and functions as an apoptosis regulator, remains undetermined. Herein, we present structural and biochemical evidence demonstrating the direct interaction between the PBM of HPV16 E6 and the PDZ domain of human PTPN4 for the first time. X-ray crystallographic structure determination and binding measurements using isothermal titration calorimetry demonstrated that hydrophobic interactions in which Leu158 of HPV16 E6 plays a key role and a network of intermolecular hydrogen bonds sustain the complex formation between PTPN4 PDZ and the PBM of HPV16 E6. In addition, it was verified that the corresponding motifs from several other highrisk HPV genotypes, including HPV18, HPV31, HPV33, and HPV45, bind to PTPN4 PDZ with comparable affinities, suggesting that PTPN4 is a common target of various pathogenic HPV genotypes.

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  • Mechanistic role of HPV-associated early proteins in cervical cancer: Molecular pathways and targeted therapeutic strategies
    Rahul Bhattacharjee, Sabya Sachi Das, Smruti Sudha Biswal, Arijit Nath, Debangshi Das, Asmita Basu, Sumira Malik, Lamha Kumar, Sulagna Kar, Sandeep Kumar Singh, Vijay Jagdish Upadhye, Danish Iqbal, Suliman Almojam, Shubhadeep Roychoudhury, Shreesh Ojha, J
    Critical Reviews in Oncology/Hematology.2022; 174: 103675.     CrossRef
Changpingibacter yushuensis gen. nov., sp. nov., isolated from fluvial sediment in Qinghai Tibet Plateau of China
Yifan Jiao , Sihui Zhang , Jing Yang , Xin-He Lai , Kui Dong , Yanpeng Cheng , Mingchao Xu , Wentao Zhu , Shan Lu , Dong Jin , Ji Pu , Ying Huang , Liyun Liu , Suping Wang , Jianguo Xu
J. Microbiol. 2022;60(2):147-155.   Published online January 7, 2022
DOI: https://doi.org/10.1007/s12275-022-1199-8
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AbstractAbstract PDF
Two facultatively anaerobic, short rod-shaped, non-motile, Gram-stain-positive, unknown bacterial strains (JY-X040T and JY-X174) were isolated from fluvial sediments of Tongtian River in Yushu Tibetan Autonomous Prefecture, Qinghai province, China. Cells formed translucent, gray, round and convex colonies, with a diameter of less than 0.5 mm after 5 days of incubation at 30°C on brain heart infusion-5% sheep blood agar. The 16S rRNA gene sequence similarity between strain JY-X040T and Fudania jinshanensis 313T is 93.87%. In the four phylogenetic trees constructed based on the 16S rRNA gene and 423 core genes, the two isolates form an independent branch, phylogenetically closest to F. jinshanensis 313T, but could not be classified as a member of the genus Fudania or any other genus of the family Arcanobacteriaceae. The DNA G + C content of strain JY-X040T was 57.8%. Calculation
results
of average nucleotide identity, digital DNADNA hybridization value and amino acid identity between strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%, and 64.1%. The major cellular fatty acids were C16:0 (23%) and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α (L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and four unidentified components. The whole-cell sugars contained rhamnose and ribose. MK-10(H4) was the sole respiratory quinone. The minimum inhibitory concentration of streptomycin was 32 μg/ml. All physiological, biochemical, chemotaxonomic and genomic characteristics support that strains JY-X040T and JY-X174 represent members of a novel species in a new genus, Changpingibacter yushuensis gen. nov., sp. nov. The type strain is JY-X040T (GDMCC 1.1996T = KCTC 49514T).

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  • Description of two novel species of the genus Chryseobacterium: Chryseobacterium chendengshani sp. nov. and Chryseobacterium wangxinyae sp. nov., isolated from soil of the Qinghai–Tibet Plateau
    Yulu Chen, Caiyun Ma, Lijun Zhao, Wenjing Lei, Jing Yang, Qiang Hou, Gui Zhang, Shuo Ning, Dong Jin, Shan Lu, Jianguo Xu
    International Journal of Systematic and Evolutionary Microbiology .2025;[Epub]     CrossRef
  • Metagenomic and Culturomics Analysis of Microbial Communities within Surface Sediments and the Prevalence of Antibiotic Resistance Genes in a Pristine River: The Zaqu River in the Lancang River Source Region, China
    Yi Yan, Jialiang Xu, Wenmin Huang, Yufeng Fan, Zhenpeng Li, Mingkai Tian, Jinsheng Ma, Xin Lu, Jian Liang
    Microorganisms.2024; 12(5): 911.     CrossRef
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    Maximilian Nepel, Veronika E. Mayer, Veronica Barrajon-Santos, Dagmar Woebken
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Direct current exerts electricidal and bioelectric effects on Porphyromonas gingivalis biofilms partially via promoting oxidative stress and antibiotic transport
Peihui Zou , Peng Li , Jia Liu , Pei Cao , Qingxian Luan
J. Microbiol. 2022;60(1):70-78.   Published online November 26, 2021
DOI: https://doi.org/10.1007/s12275-022-1238-5
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AbstractAbstract PDF
Low electric current can inhibit certain microbial biofilms and enhance the efficacy of antimicrobials against them. This study investigated the electricidal and bioelectric effects of direct current (DC) against Porphyromonas gingivalis biofilms as well as the underlying mechanisms. Here, we firstly showed that DC significantly suppressed biofilm formation of P. gingivalis in time- and intensity-dependent manners, and markedly inhibited preformed P. gingivalis biofilms. Moreover, DC enhanced the killing efficacy of metronidazole (MTZ) and amoxicillin with clavulanate potassium (AMC) against the biofilms. Notably, DC-treated biofilms displayed upregulated intracellular ROS and expression of ROS related genes (sod, feoB, and oxyR) as well as porin gene. Interestingly, DC-induced killing of biofilms was partially reversed by ROS scavenger N-dimethylthiourea (DMTU), and the synergistic effect of DC with MTZ/AMC was weakened by small interfering RNA of porin gene (si-Porin). In conclusion, DC can exert electricidal and bioelectric effects against P. gingivalis biofilms partially via promotion of oxidative stress and antibiotic transport, which offers a promising approach for effective management of periodontitis.

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    Xiaofei Xie, Chenhui Zhu, Jing Zhao, Yanru Fan, Huan Lei, Daidi Fan
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    Karthikeyani Ramesh, Sasirekha Venkidusamy, Monica Ramasamy, Rajeswari Murugesan, Arunkumar Chandrasekhar, Jeyanthinath Mayandi
    Chemical Engineering Journal.2025; 519: 164810.     CrossRef
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    X. Liu, Y. Liang, Z. Li, C. Xie, Y. Zhang, W. Wang, S. Ge, J. Li
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    Jianpeng Sun, Jingang Jiang, Zhiyuan Huang, Xuefeng Ma, Tao Shen, Jie Pan, Zhuming Bi
    Materials Today Bio.2025; 35: 102349.     CrossRef
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    Peihui Zou, Jia Liu, Peng Li, Qingxian Luan
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    Carolina Montoya, Lina Roldan, Michelle Yu, Sara Valliani, Christina Ta, Maobin Yang, Santiago Orrego
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    Zhraa F. Faruq, Sami Khalaf Jabar
    Iranian Journal of Medical Microbiology.2023; 17(6): 663.     CrossRef
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    Tao Wang, Erik Jan Cornel, Chang Li, Jianzhong Du
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  • Weak direct current exerts synergistic effect with antibiotics and reduces the antibiotic resistance: An in vitro subgingival plaque biofilm model
    Peihui Zou, Yanfeng Wang, Pei Cao, Peng Li, Jia Liu, Qingxian Luan
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  • Bioelectric device for effective biofilm inflammation management of dental implants
    Jihyun Lee, Young Wook Kim
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  • A Highly Efficacious Electrical Biofilm Treatment System for Combating Chronic Wound Bacterial Infections
    Fan Zhao, Yajuan Su, Junying Wang, Svetlana Romanova, Dominick J. DiMaio, Jingwei Xie, Siwei Zhao
    Advanced Materials.2023;[Epub]     CrossRef
Non-mitochondrial aconitase regulates the expression of iron-uptake genes by controlling the RNA turnover process in fission yeast
Soo-Yeon Cho , Soo-Jin Jung , Kyoung-Dong Kim , Jung-Hye Roe
J. Microbiol. 2021;59(12):1075-1082.   Published online October 26, 2021
DOI: https://doi.org/10.1007/s12275-021-1438-4
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AbstractAbstract PDF
Aconitase, a highly conserved protein across all domains of life, functions in converting citrate to isocitrate in the tricarboxylic acid cycle. Cytosolic aconitase is also known to act as an iron regulatory protein in mammals, binding to the RNA hairpin structures known as iron-responsive elements within the untranslated regions of specific RNAs. Aconitase-2 (Aco2) in fission yeast is a fusion protein consisting of an aconitase and a mitochondrial ribosomal protein, bL21, residing not only in mitochondria but also in cytosol and the nucleus. To investigate the role of Aco2 in the nucleus and cytoplasm of fission yeast, we analyzed the transcriptome of aco2ΔN mutant that is deleted of nuclear localization signal (NLS). RNA sequencing revealed that the aco2ΔN mutation caused increase in mRNAs encoding iron uptake transporters, such as Str1, Str3, and Shu1. The half-lives of mRNAs for these genes were found to be significantly longer in the aco2ΔN mutant than the wild-type strain, suggesting the role of Aco2 in mRNA turnover. The three conserved cysteines required for the catalytic activity of aconitase were not necessary for this role. The UV cross-linking RNA immunoprecipitation analysis revealed that Aco2 directly bound to the mRNAs of iron uptake transporters. Aco2-mediated degradation of iron-uptake mRNAs appears to utilize exoribonuclease pathway that involves Rrp6 as evidenced by genetic interactions. These results reveal a novel role of non-mitochondrial aconitase protein in the mRNA turnover in fission yeast to fine-tune iron homeostasis, independent of regulation by transcriptional repressor Fep1.

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  • Iron-mediated post-transcriptional regulation in Toxoplasma gondii
    Megan A. Sloan, Adam Scott, Dana Aghabi, Lucia Mrvova, Clare R. Harding, Dominique Soldati-Favre
    PLOS Pathogens.2025; 21(2): e1012857.     CrossRef
  • The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System Protect Yarrowia lipolytica Yeast Against pH-Induced Stress
    Tatyana I. Rakhmanova, Natalia N. Gessler, Elena P. Isakova, Olga I. Klein, Yulia I. Deryabina, Tatyana N. Popova
    Journal of Fungi.2024; 10(11): 747.     CrossRef
  • The intricate link between iron, mitochondria and azoles in Candida species
    Wouter Van Genechten, Rudy Vergauwen, Patrick Van Dijck
    The FEBS Journal.2024; 291(16): 3568.     CrossRef
  • Non-Mitochondrial Aconitase-2 Mediates the Transcription of Nuclear-Encoded Electron Transport Chain Genes in Fission Yeast
    Ho-Jung Kim, Soo-Yeon Cho, Soo-Jin Jung, Yong-Jun Cho, Jung-Hye Roe, Kyoung-Dong Kim
    Journal of Microbiology.2024; 62(8): 639.     CrossRef
  • Kinetic and Regulatory Properties of Yarrowia lipolytica Aconitate Hydratase as a Model-Indicator of Cell Redox State under pH Stress
    Tatyana I. Rakhmanova, Varvara Yu. Sekova, Natalya N. Gessler, Elena P. Isakova, Yulia I. Deryabina, Tatyana N. Popova, Yevgeniya I. Shurubor, Boris F. Krasnikov
    International Journal of Molecular Sciences.2023; 24(8): 7670.     CrossRef
A study of P release from Fe-P and Ca-P via the organic acids secreted by Aspergillus niger
Da Tian , Liyan Wang , Jun Hu , Liangliang Zhang , Ningning Zhou , Jingjing Xia , Meiyue Xu , Kianpoor Kalkhajeh Yusef , Shimei Wang , Zhen Li , Hongjian Gao
J. Microbiol. 2021;59(9):819-826.   Published online August 12, 2021
DOI: https://doi.org/10.1007/s12275-021-1178-5
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AbstractAbstract PDF
Phosphate solubilizing fungi (PSF) have been widely applied to dissolve insoluble phosphates (IPs). However, the PSF usually demonstrates a different phosphate solubilizing capacity for various IPs. This study explored the mechanisms of Aspergillus niger for the dissolution of ferric phosphate (FePO4, Fe-P), and tricalcium phosphate (Ca3[PO4]2, Ca-P) regarding the tricarboxylic acid (TCA) cycle. Aspergillus niger has higher phosphorus (P) content released from Ca-P, reached the maximum value of 861 mg/L after seven days of incubation, compared with the 169 mg/L from Fe-P. Oxalic acid promoted the release of P from Ca-P through the formation of calcium oxalate. The presence of Fe-P can stimulate A. niger to secrete large amounts of citric acid, confirmed by the enhancement of citrate synthase (CS) activity. However, citric acid only promotes 0.5% of P released from Fe-P. Meanwhile, although oxalic acid still dominates the release of P from Fe-P, its abundance was significantly declined. In contrast, oxalic acid also shows a higher P release ratio in Ca-P than citric acid, i.e., 36% vs. 22%. This study points to the future usage of A. niger to dissolve IPs in soil required to enhance oxalic acid secretion.

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    Da Tian, Jingjing Xia, Ningning Zhou, Meiyue Xu, Xiang Li, Liangliang Zhang, Shuhua Du, Hongjian Gao
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    Yi Feng, Liangliang Zhang, Xiang Li, Liyan Wang, Kianpoor Kalkhajeh Yusef, Hongjian Gao, Da Tian
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  • Aspergillus nigerEnhances Organic and Inorganic Phosphorus Release from Wheat Straw by Secretion of Degrading Enzymes and Oxalic Acid
    Liyan Wang, Hao Guan, Jun Hu, Yi Feng, Xiang Li, Kianpoor Kalkhajeh Yusef, Hongjian Gao, Da Tian
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  • A Study of Phosphate Solubilizing Capacity by Penicillium Aurantiogriseum under Different Carbon and Nitrogen Resources
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Incomplete autophagy promotes the replication of Mycoplasma hyopneumoniae
Zhaodi Wang† , Yukang Wen† , Bingqian Zhou , Yaqin Tian , Yaru Ning , Honglei Ding
J. Microbiol. 2021;59(8):782-792.   Published online July 5, 2021
DOI: https://doi.org/10.1007/s12275-021-1232-3
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AbstractAbstract PDF
Autophagy is an important cellular homeostatic mechanism for recycling of degradative proteins and damaged organelles. Autophagy has been shown to play an important role in cellular responses to bacteria and bacterial replication. However, the role of autophagy in Mycoplasma hyopneumoniae infection and the pathogenic mechanism is not well characterized. In this study, we showed that M. hyopneumoniae infection significantly increases the number of autophagic vacuoles in host cells. Further, we found significantly enhanced expressions of autophagy marker proteins (LC3-II, ATG5, and Beclin 1) in M. hyopneumoniae-infected cells. Moreover, immunofluorescence analysis showed colocalization of P97 protein with LC3 during M. hyopneumoniae infection. Interestingly, autophagic flux marker, p62, accumulated with the induction of infection. Conversely, the levels of p62 and LC3-II were decreased after treatment with 3-MA, inhibiting the formation of autophagosomes, during infection. In addition, accumulation of autophagosomes promoted the expression of P97 protein and the survival of M. hyopneumoniae in PK- 15 cells, as the replication of M. hyopneumoniae was downregulated by adding 3-MA. Collectively, these findings provide strong evidence that M. hyopneumoniae induces incomplete autophagy, which in turn enhances its reproduction in host cells. These findings provide novel insights into the interaction of M. hyopneumoniae and host.

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    Huijie Zhao, Yihan Yang, Xinya Si, Huiyang Liu, Honggang Wang
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  • Mycoplasma bovis inhibits autophagy in bovine mammary epithelial cells via a PTEN/PI3K-Akt-mTOR-dependent pathway
    Maolin Xu, Yang Liu, Tuerdi Mayinuer, Yushan Lin, Yue Wang, Jian Gao, Dong Wang, John P. Kastelic, Bo Han
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  • Incomplete autophagy promotes the proliferation of Mycoplasma hyopneumoniae through the JNK and Akt pathways in porcine alveolar macrophages
    Yukang Wen, Zhengkun Chen, Yaqin Tian, Mei Yang, Qingshuang Dong, Yujiao Yang, Honglei Ding
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CagL polymorphisms between East Asian and Western Helicobacter pylori are associated with different abilities to induce IL-8 secretion
Yun Hui Choi , Jing Lai , Myeong-A Kim , Aeryun Kim , Jinmoon Kim , Hanfu Su , Linhu Ge , Jeong-Heon Cha
J. Microbiol. 2021;59(8):763-770.   Published online June 1, 2021
DOI: https://doi.org/10.1007/s12275-021-1136-2
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AbstractAbstract PDF
Helicobacter pylori colonizes human gastric mucosa. Its infection is associated with gastric diseases including gastric cancer. CagA is one of the most important toxins produced by H. pylori. It is related to gastric cancer which can be injected into host cells via a type IV secretion system (T4SS). CagL is a structural component of T4SS apparatus, which triggers host cell signaling pathway. It has been reported that CagL polymorphisms may influence the severity of disease development. To explore the contribution of CagL polymorphisms between East Asian and Western H. pylori in pathogenesis, cagL gene in G27 H. pylori was swapped by K74 cagL which is identical to East Asian CagL consensus sequence and by Western 26695 H. pylori, resulting in G27ΔcagL/cagLK74 and G27ΔcagL/cagL26695, respectively. Intriguingly, G27ΔcagL/ cagLK74 showed significantly less ability of IL-8 induction than G27ΔcagL/cagL26695 while displayed similar abilities of CagA phosphorylation, and cell elongation. Taken together, this study suggests that the CagL polymorphism may influence IL-8 induction, and K74 CagL has less ability to induce IL-8 secretion than G27 or 26695 CagL. Further research should address how the different capabilities of IL-8 induction between intraspecies-CagL are associated with the large differences of the incidence of gastric cancer between East Asian and Western countries.

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  • Roles of the components of the cag -pathogenicity island encoded type IV secretion system in Helicobacter pylori
    Lingzhu Gou, Xiaoping Yang, Jianwei Yun, Zenghui Ma, Xiaofeng Zheng, Hongwei Du, Dekui Zhang
    Future Microbiology.2024; 19(14): 1253.     CrossRef
  • Antibacterial and Immunoregulatory Effects of Metformin against Helicobacter pylori Infection in Rat Model
    Hassan Valadbeigi, Saeed Khoshnood, Babak Negahdari, Mohd Azmuddin Abdullah, Mohammad Hossein Haddadi, Abhimanyu Abhimanyu
    BioMed Research International.2023;[Epub]     CrossRef
  • New CagL Amino Acid Polymorphism Patterns of Helicobacter pylori in Peptic Ulcer and Non-Ulcer Dyspepsia
    Reyhan Caliskan, Silva Polat Sari, Bahadir Ercan, Kivanc Derya Peker, Mehtap Omac Sonmez, Ozer Akgul, Burcu Sapmaz, Aliye Soylu, Gokhan Tolga Adas, Yasar Ali Oner, Pelin Yuksel Mayda
    Medicina.2022; 58(12): 1738.     CrossRef
  • Geographic diversity in Helicobacter pylori oipA genotype between Korean and United States isolates
    Aeryun Kim, Jing Lai, D. Scott Merrell, Ji-Hye Kim, Hanfu Su, Jeong-Heon Cha
    Journal of Microbiology.2021; 59(12): 1125.     CrossRef
Type 2 human papillomavirus E7 attenuates E-cadherin expression in human keratinocytes
Ji Young Song , Young Min Park , Soon Yong Choi
J. Microbiol. 2021;59(6):616-625.   Published online March 29, 2021
DOI: https://doi.org/10.1007/s12275-021-0690-y
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AbstractAbstract PDF
Human papillomaviruses (HPVs) are known to utilize the down-regulation of epithelial (E)-cadherin, a major component of adherens junctions of keratinocytes, to evade host immune surveillance in high-risk group. However, the effects of HPV on the function of E-cadherin in low-risk groups remain unknown. We investigated whether type 2 HPV (HPV- 2) E7 could induce alterations in E-cadherin expression in transiently transfected keratinocytes and cell lines expressing HPV-2 E7. To examine the expression pattern of E-cadherin in cutaneous warts and normal skin samples, immunohistochemical analysis was performed. Quantitative real-time polymerase chain reactions, luciferase assays, western blot, immunocytochemistry, and electron microscopy were used to evaluate the mRNA and protein expression levels of Ecadherin in normal human epidermal keratinocytes transfected with HPV-2 E7 plasmid DNA or E7-specific siRNA and in E7-expressing cell lines. E-cadherin expression levels in HPV-2 positive cutaneous warts were significantly decreased compared to those in normal skin (p < 0.05). Similarly, the mRNA and protein expression levels of E-cadherin in E7 transiently transfected cells were significantly decreased compared to those in empty vector-transfected cells. The decreases were restored by transfection with E7-specific siRNA (p < 0.05). Likewise, cell lines expressing E7 showed a decreased expression of E-cadherin. When the cells were cultured in low attachment plates, cell-to-cell aggregation was inhibited. Taken together, our data suggest that HPV-2 E7, the causative agent of cutaneous warts, could mediate the transcriptional repression of E-cadherin.

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  • The NLRP3 inflammasome in viral infection (Review)
    Qiaoli Zheng, Chunting Hua, Qichang Liang, Hao Cheng
    Molecular Medicine Reports.2023;[Epub]     CrossRef
Molecular mechanism of Escherichia coli H10407 induced diarrhoea and its control through immunomodulatory action of bioactives from Simarouba amara (Aubl.)
Hegde Veena , Sandesh K. Gowda , Rajeshwara N. Achur , Nayaka Boramuthi Thippeswamy
J. Microbiol. 2021;59(4):435-447.   Published online February 25, 2021
DOI: https://doi.org/10.1007/s12275-021-0423-2
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AbstractAbstract PDF
Enterotoxigenic Escherichia coli (ETEC) infection is a major cause of death in children under the age of five in developing countries. ETEC (O78:H11:CFA/I:LT+:ST+) mechanism has been studied in detail with either heat labile (LT) or heat stable (ST) toxins using in vitro and in vivo models. However, there is no adequate information on ETEC pathogenesis producing both the toxins (LT, ST) in BALB/c mice model. In this study, female mice have been employed to understand ETEC H10407 infection induced changes in physiology, biochemical and immunological patterns up to seven days post-infection and the antidiarrhoeal effect of Simarouba amara (Aubl.) bark aqueous extract (SAAE) has also been looked into. The results indicate that BALB/c is sensitive to ETEC infection resulting in altered jejunum and ileum histomorphology. Withal, ETEC influenced cAMP, PGE2, and NO production resulting in fluid accumulation with varied Na+, K+, Cl-, and Ca2+ levels. Meanwhile, ETEC subverted expression of IL-1β, intestine alkaline phosphatase (IAP), and myeloperoxidase (MPO) in jejunum and ileum. Our data also indicate the severity of pathogenesis reduction which might be due to attainment of equilibrium after reaching optimum rate of infection. Nevertheless, degree of pathogenesis was highly significant (p < 0.01) in all the studied parameters. Besides that, SAAE was successful in reducing the infectious diarrhoea by inhibiting ETEC H10407 in intestine (jejunum and ileum), and shedding in feces. SAAE decreased cAMP, PGE2, and fluid accumulation effectively and boosted the functional activity of immune system in jejunum and ileum IAP, MPO, IL-1β, and nitric oxide.

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  • Relaxed Cleavage Specificity of Hyperactive Variants of Escherichia coli RNase E on RNA I
    Dayeong Bae, Hana Hyeon, Eunkyoung Shin, Ji-Hyun Yeom, Kangseok Lee
    Journal of Microbiology.2023; 61(2): 211.     CrossRef
  • A systematic antidiarrhoeal evaluation of a vegetable root Begonia roxburghii and its marker flavonoids against nonpathogenic and pathogenic diarrhoea
    Rupali S. Prasad, Nikhil Y. Yenorkar, Suhas R. Dhaswadikar, Saurabh K. Sinha, Nitish Rai, Pravesh Sharma, Onkar Kulkarni, Neeraj Kumar, Mahaveer Dhobi, Damiki Laloo, Shailendra S. Gurav, Prakash R. Itankar, Satyendra K. Prasad
    Food Bioscience.2023; 53: 102672.     CrossRef
Alcohol dehydrogenase 1 and NAD(H)-linked methylglyoxal oxidoreductase reciprocally regulate glutathione-dependent enzyme activities in Candida albicans
Sa-Ouk Kang , Min-Kyu Kwak
J. Microbiol. 2021;59(1):76-91.   Published online December 23, 2020
DOI: https://doi.org/10.1007/s12275-021-0552-7
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AbstractAbstract PDF
Glutathione reductase (Glr1) activity controls cellular glutathione and reactive oxygen species (ROS). We previously demonstrated two predominant methylglyoxal scavengers– NAD(H)-linked methylglyoxal oxidoreductase (Mgd1) and alcohol dehydrogenase 1 (Adh1)–in glutathione-depleted γ- glutamyl cysteinyl synthetase-disrupted Candida albicans. However, experimental evidence for Candida pathophysiology lacking the enzyme activities of Mgd1 and Adh1 on glutathione- dependent redox regulation remains unclear. Herein, we have aimed to demonstrate that glutathione-dependent enzyme activities coupled with cellular ROS changes is regulated by methylglyoxal accumulation in Δmgd1/Δadh1 double disruptants. Δmgd1/Δadh1 showed severe growth defects and G1-phase cell cycle arrest. The observed complementary and reciprocal methylglyoxal-oxidizing and methylglyoxalreducing activities between Δmgd1 and Δadh1 were not always exhibited in Δmgd1/Δadh1. Although intracellular accumulation of methylglyoxal and pyruvate was shown in all disruptants, to a greater or lesser degree, methylglyoxal was particularly accumulated in the Δmgd1/Δadh1 double disruptant. While cellular ROS significantly increased in Δmgd1 and Δadh1 as compared to the wild-type, Δmgd1/Δadh1 underwent a decrease in ROS in contrast to Δadh1. Despite the experimental findings underlining the importance of the undergoing unbalanced redox state of Δmgd1/Δadh1, glutathione- independent antioxidative enzyme activities did not change during proliferation and filamentation. Contrary to the significantly lowered glutathione content and Glr1 enzyme activity, the activity staining-based glutathione peroxidase activities concomitantly increased in this mutant. Additionally, the enhanced GLR1 transcript supported our results in Δmgd1/Δadh1, indicating that deficiencies of both Adh1 and Mgd1 activities stimulate specific glutathione-dependent enzyme activities. This suggests that glutathione-dependent redox regulation is evidently linked to C. albicans pathogenicity under the control of methylglyoxal-scavenging activities.

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  • Roles of alcohol dehydrogenase 1 in the biological activities of Candida albicans
    Ziqi Wang, Qi Zhang, Haoying Zhang, Yuanyuan Lu
    Critical Reviews in Microbiology.2025; 51(3): 484.     CrossRef
  • Antifungal effects of Metformin against Candida albicans by autophagy regulation
    Xiao Zhao, Yang Wang, Qinqin Zhang, Yun Huang, Xin Wei, Daming Wu
    Journal of Microbiology.2025; 63(4): e2411008.     CrossRef
  • Role of methylglyoxal and redox homeostasis in microbe-mediated stress mitigation in plants
    Sampurna Garai, Bidisha Bhowal, Mayank Gupta, Sudhir K Sopory, Sneh L. Singla-Pareek, Ashwani Pareek, Charanpreet Kaur
    Plant Science.2024; 338: 111922.     CrossRef
Description of Vagococcus coleopterorum sp. nov., isolated from the intestine of the diving beetle, Cybister lewisianus, and Vagococcus hydrophili sp. nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus, and emended description of the genus Vagococcus
Dong-Wook Hyun , Euon Jung Tak , Pil Soo Kim , Jin-Woo Bae
J. Microbiol. 2021;59(2):132-141.   Published online December 23, 2020
DOI: https://doi.org/10.1007/s12275-021-0485-1
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AbstractAbstract PDF
A polyphasic taxonomic approach was used to characterize two novel bacterial strains, HDW17AT and HDW17BT, isolated from the intestine of the diving beetle Cybister lewisianus, and the dark diving beetle Hydrophilus acuminatus, respectively. Both strains were Gram-positive and facultative anaerobic cocci forming cream-colored colonies. The isolates grew optimally at 25°C, pH 7, in the presence of 0.3% (wt/vol) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolates were members of the genus Vagococcus, and strain HDW17AT was closely related to Vagococcus fessus CCUG 41755T (98.9% of 16S rRNA gene sequence similarity and 74.3% of average nucleotide identity [ANI]), whereas strain HDW17BT was closely related to Vagococcus fluvialis NCFB 2497T (98.9% of 16S rRNA gene sequence similarity and 76.6% of ANI). Both strains contained C16:0, and C18:1 ω9c as the major cellular fatty acids, but C16:1 ω9c was also observed only in strain HDW17BT as the major cellular fatty acid. The respiratory quinone of the isolates was MK-7. The major polar lipid components were phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. The genomic DNA G + C content of strains HDW17AT and HDW17BT were 36.6 and 34.4%, respectively. Both strains had cell wall peptidoglycan composed of the amino acids L-alanine, glycine, D-glutamic acid, L-tryptophan, L-lysine, and L-aspartic acid, and the sugars ribose, glucose, and galactose. Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW17AT and HDW17BT represent two novel species in the genus Vagococcus. We propose the name Vagococcus coleopterorum sp. nov. for strain HDW17AT (= KACC 21348T = KCTC 49324T = JCM 33674T) and the name Vagococcus hydrophili sp. nov. for strain HDW17BT (= KACC 21349T = KCTC 49325T = JCM 33675T).

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  • Whole-Genome Sequencing and Genomic Features of Vagococcus sp. JNUCC 83 Isolated from Camellia japonica Flowers
    Kyung-A Hyun, Ji-Hyun Kim, Min Nyeong Ko, Chang-Gu Hyun
    Microbiology Research.2026; 17(1): 23.     CrossRef
  • Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged broiler meat
    Per Johansson, Elina Jääskeläinen, Elina Säde, Johanna Björkroth
    International Journal of Systematic and Evolutionary Microbiology .2023;[Epub]     CrossRef
  • A case of Vagococcus fluvialis isolated from the bile of a patient with calculous cholecystitis
    Dan Zhang, Xiaosu Wang, Jingdan Yu, Zheng Dai, Qichao Li, Litao Zhang
    BMC Infectious Diseases.2023;[Epub]     CrossRef
  • Vagococcus luciliae sp. nov., isolated from the common green bottle fly Lucilia sericata
    Juan Guzman, Anja Poehlein, Rolf Daniel, Peter Kämpfer, Andreas Vilcinskas
    International Journal of Systematic and Evolutionary Microbiology .2023;[Epub]     CrossRef
  • Valid publication of new names and new combinations effectively published outside the IJSEM. Validation List no. 203
    Aharon Oren, George M. Garrity
    International Journal of Systematic and Evolutionary Microbiology .2022;[Epub]     CrossRef
  • Effects of different doses of electron beam irradiation on bacterial community of Portunus trituberculatus
    Huijuan Pan, Qi Yu, Chenru Qian, Haitao Shao, Jiajun Han, Yongyong Li, Yongjiang Lou
    Food Bioscience.2021; 42: 101198.     CrossRef
  • Description of Nocardioides piscis sp. nov., Sphingomonas piscis sp. nov. and Sphingomonas sinipercae sp. nov., isolated from the intestine of fish species Odontobutis interrupta (Korean spotted sleeper) and Siniperca scherzeri (leopard mandarin fish)
    Dong-Wook Hyun, Yun-Seok Jeong, Jae-Yun Lee, Hojun Sung, So-Yeon Lee, Jee-Won Choi, Hyun Sik Kim, Pil Soo Kim, Jin-Woo Bae
    Journal of Microbiology.2021; 59(6): 552.     CrossRef
Soil water content as a critical factor for stable bacterial community structure and degradative activity in maritime Antarctic soil
Dockyu Kim , Namyi Chae , Mincheol Kim , Sungjin Nam , Eungbin Kim , Hyoungseok Lee
J. Microbiol. 2020;58(12):1010-1017.   Published online December 2, 2020
DOI: https://doi.org/10.1007/s12275-020-0490-9
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AbstractAbstract PDF
Recent increases in air temperature across the Antarctic Peninsula may prolong the thawing period and directly affect the soil temperature (Ts) and volumetric soil water content (SWC) in maritime tundra. Under an 8°C soil warming scenario, two customized microcosm systems with maritime Antarctic soils were incubated to investigate the differential influence of SWC on the bacterial community and degradation activity of humic substances (HS), the largest constituent of soil organic carbon and a key component of the terrestrial ecosystem. When the microcosm soil (KS1-4Feb) was incubated for 90 days (T = 90) at a constant SWC of ~32%, the initial HS content (167.0 mg/g of dried soil) decreased to 156.0 mg (approximately 6.6% loss, p < 0.05). However, when another microcosm soil (KS1-4Apr) was incubated with SWCs that gradually decreased from 37% to 9% for T = 90, HS degradation was undetected. The low HS degradative activity persisted, even after the SWC was restored to 30% with water supply for an additional T = 30. Overall bacterial community structure remained relatively stable at a constant SWC setting (KS1-4Feb). In contrast, we saw marked shifts in the bacterial community structure with the changing SWC regimen (KS1-4Apr), suggesting that the soil bacterial communities are vulnerable to drying and re-wetting conditions. These microcosm experiments provide new information regarding the effects of constant SWC and higher Ts on bacterial communities for HS degradation in maritime Antarctic tundra soil.

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    Yi-He Zhang, Yong-Qiang Hu, Yin-Xin Zeng, Ting Hu, Wei Han, Yu Du, Zhong Hu, Shan-Shan Meng
    Frontiers in Microbiology.2025;[Epub]     CrossRef
  • Effect of coal mining subsidence on soil microbial communities in mining areas with high groundwater levels
    Ruiping Xu, Junying Li, Xinju Li, Wen Song
    Environmental Geochemistry and Health.2025;[Epub]     CrossRef
  • Influence of Composite Amendments on the Characteristics of Sandy Soil
    Xinrui Sui, Lingyan Wang, Xinyao Lv, Yanan Liu, Yuqi Zhu, Lingyun Fan, Hanxi Wang
    Sustainability.2025; 17(17): 7619.     CrossRef
  • Microbial metabolic responses and CO2 emissions differentiated by soil water content variation in subarctic tundra soils
    Dockyu Kim, Namyi Chae, Mincheol Kim, Sungjin Nam, Tai Kyoung Kim, Ki-Tea Park, Bang Yong Lee, Eungbin Kim, Hyoungseok Lee
    Journal of Microbiology.2022; 60(12): 1130.     CrossRef
  • Temperature sensitivity of Antarctic soil‐humic substance degradation by cold‐adapted bacteria
    Dockyu Kim, Ha Ju Park, Mincheol Kim, Seulah Lee, Soon Gyu Hong, Eungbin Kim, Hyoungseok Lee
    Environmental Microbiology.2022; 24(1): 265.     CrossRef
  • Seasonal patterns of rhizosphere microorganisms suggest carbohydrate-degrading and nitrogen-fixing microbes contribute to the attribute of full-year shooting in woody bamboo Cephalostachyum pingbianense
    Lushuang Li, Tize Xia, Hanqi Yang
    Frontiers in Microbiology.2022;[Epub]     CrossRef
The synergy effect of arbuscular mycorrhizal fungi symbiosis and exogenous calcium on bacterial community composition and growth performance of peanut (Arachis hypogaea L.) in saline alkali soil
Dunwei Ci , Zhaohui Tang , Hong Ding , Li Cui , Guanchu Zhang , Shangxia Li , Liangxiang Dai , Feifei Qin , Zhimeng Zhang , Jishun Yang , Yang Xu
J. Microbiol. 2021;59(1):51-63.   Published online November 17, 2020
DOI: https://doi.org/10.1007/s12275-021-0317-3
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AbstractAbstract PDF
Peanut (Arachis hypogaea. L) is an important oil seed crop. Both arbuscular mycorrhizal fungi (AMF) symbiosis and calcium (Ca2+) application can ameliorate the impact of saline soil on peanut production, and the rhizosphere bacterial communities are also closely correlated with peanut salt tolerance; however, whether AMF and Ca2+ can withstand high-salinity through or partially through modulating rhizosphere bacterial communities is unclear. Here, we used the rhizosphere bacterial DNA from saline alkali soil treated with AMF and Ca2+ alone or together to perform high-throughput sequencing of 16S rRNA genes. Taxonomic analysis revealed that AMF and Ca2+ treatment increased the abundance of Proteobacteria and Firmicutes at the phylum level. The nitrogenfixing bacterium Sphingomonas was the dominant genus in these soils at the genus level, and the soil invertase and urease activities were also increased after AMF and Ca2+ treatment, implying that AMF and Ca2+ effectively improved the living environment of plants under salt stress. Moreover, AMF combined with Ca2+ was better than AMF or Ca2+ alone at altering the bacterial structure and improving peanut growth in saline alkali soil. Together, AMF and Ca2+ applications are conducive to peanut salt adaption by regulating the bacterial community in saline alkali soil.

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    Rhizosphere.2026; 37: 101238.     CrossRef
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    Plant Physiology and Biochemistry.2026; 230: 110849.     CrossRef
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    BMC Plant Biology.2022;[Epub]     CrossRef
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    International Journal of Molecular Sciences.2022; 23(10): 5309.     CrossRef
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