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- Saccharomyces cerevisiae Cmr1 Protein Preferentially Binds to UV-Damaged DNA In Vitro
- Do-Hee Choi , Sung-Hun Kwon , Joon-Ho Kim , Sung-Ho Bae
- J. Microbiol. 2012;50(1):112-118. Published online February 27, 2012
- DOI: https://doi.org/10.1007/s12275-012-1597-4
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- 10 Crossref
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Abstract
PDF -
DNA metabolic processes such as DNA replication, recombination,
and repair are fundamentally important for the
maintenance of genome integrity and cell viability. Although
a large number of proteins involved in these pathways have
been extensively studied, many proteins still remain to be
identified. In this study, we isolated DNA-binding proteins
from Saccharomyces cerevisiae using DNA-cellulose columns.
By analyzing the proteins using mass spectrometry, an uncharacterized
protein, Cmr1/YDL156W, was identified. Cmr1
showed sequence homology to human Damaged-DNA binding
protein 2 in its C-terminal WD40 repeats. Consistent
with this finding, the purified recombinant Cmr1 protein
was found to be intrinsically associated with DNA-binding
activity and exhibited higher affinity to UV-damaged DNA
substrates. Chromatin isolation experiments revealed that
Cmr1 localized in both the chromatin and supernatant
fractions, and the level of Cmr1 in the chromatin fraction
increased when yeast cells were irradiated with UV. These
results
suggest that Cmr1 may be involved in DNA-damage responses in yeast. -
Citations
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- An integrated structural model of the DNA damage-responsive H3K4me3 binding WDR76:SPIN1 complex with the nucleosome
Xingyu Liu, Ying Zhang, Zhihui Wen, Yan Hao, Charles A.S. Banks, Joseph Cesare, Saikat Bhattacharya, Shreyas Arvindekar, Jeffrey J. Lange, Yixuan Xie, Benjamin A. Garcia, Brian D. Slaughter, Jay R. Unruh, Shruthi Viswanath, Laurence Florens, Jerry L. Work
Proceedings of the National Academy of Sciences.2024;[Epub] CrossRef - Histone H4 dosage modulates DNA damage response in the pathogenic yeast Candida glabrata via homologous recombination pathway
Kundan Kumar, Romila Moirangthem, Rupinder Kaur, Michael Freitag
PLOS Genetics.2020; 16(3): e1008620. CrossRef - Genome Wide Analysis of WD40 Proteins in Saccharomyces cerevisiae and Their Orthologs in Candida albicans
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The Protein Journal.2019; 38(1): 58. CrossRef - Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome
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Jeffery W. Jones, Priyanka Singh, Chhabi K. Govind, Sukesh R. Bhaumik
PLOS ONE.2016; 11(2): e0148897. CrossRef - Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control
Irene Gallina, Camilla Colding, Peter Henriksen, Petra Beli, Kyosuke Nakamura, Judith Offman, David P. Mathiasen, Sonia Silva, Eva Hoffmann, Anja Groth, Chunaram Choudhary, Michael Lisby
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The EMBO Journal.2015; 34(6): 778. CrossRef - Yeast gene CMR1/YDL156W is consistently co-expressed with genes participating in DNA-metabolic processes in a variety of stringent clustering experiments
Basel Abu-Jamous, Rui Fa, David J. Roberts, Asoke K. Nandi
Journal of The Royal Society Interface.2013; 10(81): 20120990. CrossRef - Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress
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Nature Cell Biology.2012; 14(9): 966. CrossRef
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