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- Synthetic biology strategies for sustainable bioplastic production by yeasts
- Huong-Giang Le, Yongjae Lee, Sun-Mi Lee
- J. Microbiol. 2025;63(3):e2501022. Published online March 28, 2025
- DOI: https://doi.org/10.71150/jm.2501022
- 541 View
- 35 Download
- 1 Crossref
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Abstract
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The increasing environmental concerns regarding conventional plastics have led to a growing demand for sustainable alternatives, such as biodegradable plastics. Yeast cell factories, specifically Saccharomyces cerevisiae and Yarrowia lipolytica, have emerged as promising platforms for bioplastic production due to their scalability, robustness, and ease of manipulation. This review highlights synthetic biology approaches aimed at developing yeast cell factories to produce key biodegradable plastics, including polylactic acid (PLA), polyhydroxyalkanoates (PHAs), and poly (butylene adipate-co-terephthalate) (PBAT). We explore recent advancements in engineered yeast strains that utilize various synthetic biology strategies, such as the incorporation of new genetic elements at the gene, pathway, and cellular system levels. The combined efforts of metabolic engineering, protein engineering, and adaptive evolution have enhanced strain efficiency and maximized product yields. Additionally, this review addresses the importance of integrating computational tools and machine learning into the Design-Build-Test-Learn cycle for strain development. This integration aims to facilitate strain development while minimizing effort and maximizing performance. However, challenges remain in improving strain robustness and scaling up industrial production processes. By combining advanced synthetic biology techniques with computational approaches, yeast cell factories hold significant potential for the sustainable and scalable production of bioplastics, thus contributing to a greener bioeconomy.
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Citations
Citations to this article as recorded by
- Advancing microbial engineering through synthetic biology
Ki Jun Jeong
Journal of Microbiology.2025; 63(3): e2503100. CrossRef
- Advancing microbial engineering through synthetic biology
- Screening of small molecules attenuating biofilm formation of Acinetobacter baumannii by inhibition of ompA promoter activity
- Seok Hyeon Na , Hyejin Jeon , Man Hwan Oh , Yoo Jeong Kim , Je Chul Lee
- J. Microbiol. 2021;59(9):871-878. Published online August 27, 2021
- DOI: https://doi.org/10.1007/s12275-021-1394-z
- 63 View
- 0 Download
- 12 Web of Science
- 12 Crossref
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Abstract
- Anti-virulence therapeutic strategies are promising alternatives against drug-resistant pathogens. Outer membrane protein A (OmpA) plays a versatile role in the pathogenesis and antimicrobial resistance of Acinetobacter baumannii. Therefore, OmpA is an innovative target for anti-virulence therapy against A. baumannii. This study aimed to develop a high-throughput screening (HTS) system to discover small molecules inhibiting the ompA promoter activity of A. baumannii and screen chemical compounds using the bacterial growth-based HTS system. The ompA promoter and open reading frame of nptI fusion plasmids that controlled the expression of nptI encoding resistance to kanamycin by the ompA promoter were constructed and then transformed into A. baumannii ATCC 17978. This reporter strain was applied to screen small molecules inhibiting the ompA promoter activity in a chemical library. Of the 7,520 chemical compounds, 15 exhibited ≥ 70% growth inhibition of the report strain cultured in media containing kanamycin. Three compounds inhibited the expression of ompA and OmpA in the outer membrane of A. baumannii ATCC 17978, which subsequently reduced biofilm formation. In conclusion, our reporter strain is useful for large-scale screening of small molecules inhibiting the ompA expression in A. baumannii. Hit compounds identified by the HTS system are promising scaffolds to develop novel therapeutics against A. baumannii.
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Citations
Citations to this article as recorded by-
A peptide targeting outer membrane protein A of
Acinetobacter baumannii
exhibits antibacterial activity by reducing bacterial pathogenicity
Hui Zhao, Yue Hu, Dan Nie, Na Li, Zhou Chen, Shan Zhou, Mingkai Li, Xiaoyan Xue, James E. Leggett
Antimicrobial Agents and Chemotherapy.2024;[Epub] CrossRef - Acinetobacter baumannii OmpA-like porins: functional characterization of bacterial physiology, antibiotic-resistance, and virulence
Daniela Scribano, Elena Cheri, Arianna Pompilio, Giovanni Di Bonaventura, Manuel Belli, Mario Cristina, Luigi Sansone, Carlo Zagaglia, Meysam Sarshar, Anna Teresa Palamara, Cecilia Ambrosi
Communications Biology.2024;[Epub] CrossRef - Anti-OmpA antibodies as potential inhibitors of Acinetobacter baumannii biofilm formation, adherence to, and proliferation in A549 human alveolar epithelial cells
Hamideh Barati, Zahra Fekrirad, Mohammadreza Jalali Nadoushan, Iraj Rasooli
Microbial Pathogenesis.2024; 186: 106473. CrossRef -
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Critical Reviews in Microbiology.2024; : 1. CrossRef - Understanding the mechanisms of antimicrobial resistance and potential therapeutic approaches against the Gram-negative pathogen Acinetobacter baumannii
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RSC Medicinal Chemistry.2024; 15(12): 3925. CrossRef - Acinetobacter baumannii outer membrane protein A induces autophagy in bone marrow‐derived dendritic cells involving the PI3K/mTOR pathway
Hongyi Tan, Liyan Cao
Immunity, Inflammation and Disease.2023;[Epub] CrossRef - Advances in research on virulence factors ofAcinetobacter baumanniiand their potential as novel therapeutic targets
Jian-Xia Zhou, Ding-Yun Feng, Xia Li, Jia-Xin Zhu, Wen-Bin Wu, Tian-tuo Zhang
Journal of Applied Microbiology.2023;[Epub] CrossRef - Famotidine Enhances Rifampicin Activity against Acinetobacter baumannii by Affecting OmpA
Meng-na Zhang, Xiao-ou Zhao, Qi Cui, Dao-mi Zhu, Muhammad Asif Wisal, Han-dong Yu, Ling-cong Kong, Hong-xia Ma, Laurie E. Comstock
Journal of Bacteriology.2023;[Epub] CrossRef - Factors mediating Acinetobacter baumannii biofilm formation: Opportunities for developing therapeutics
Kirti Upmanyu, Qazi Mohd. Rizwanul Haq, Ruchi Singh
Current Research in Microbial Sciences.2022; 3: 100131. CrossRef - Evaluation the reactivity of a peptide-based monoclonal antibody derived from OmpA with drug resistant pulsotypes of Acinetobacter baumannii as a potential therapeutic approach
Omid Yeganeh, Mahdi Shabani, Parviz Pakzad, Nariman Mosaffa, Ali Hashemi
Annals of Clinical Microbiology and Antimicrobials.2022;[Epub] CrossRef - Therapeutic Effects of Inhibitor of ompA Expression against Carbapenem-Resistant Acinetobacter baumannii Strains
Seok-Hyeon Na, Hyejin Jeon, Man-Hwan Oh, Yoo-Jeong Kim, Mingi Chu, Ill-Young Lee, Je-Chul Lee
International Journal of Molecular Sciences.2021; 22(22): 12257. CrossRef - DksA Modulates Antimicrobial Susceptibility of Acinetobacter baumannii
Nayeong Kim, Joo-Hee Son, Kyeongmin Kim, Hyo-Jeong Kim, Minsang Shin, Je-Chul Lee
Antibiotics.2021; 10(12): 1472. CrossRef
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A peptide targeting outer membrane protein A of
Acinetobacter baumannii
exhibits antibacterial activity by reducing bacterial pathogenicity
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